scholarly journals A Metal Impregnation Method of Biological Specimens for Scanning Electron Microscopy

1973 ◽  
Vol 35 (4) ◽  
pp. 323-326 ◽  
Author(s):  
Takuro MURAKAMI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Impregnation Method ◽  
Metal Impregnation ◽  
Scanning Electron

Surface Morphology and the Adsorptive Behavior of Coconut Active Carbon

2013 ◽  
Vol 690-693 ◽  
pp. 1029-1032
Author(s):  
Xiao Qiang Li ◽  
Li Juan Lan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Hydrochloric Acid ◽  
Active Carbon ◽  
Adsorption Process ◽  
Hydrochloric Acid Concentration ◽  
Impregnation Method ◽  
Adsorption Experiment ◽  
Adsorptive Behavior ◽  
Scanning Electron

Active carbon modified by hydrochloric acid was prepared with impregnation method. The synthesized material was characterized by scanning electron microscopy. It was proved that plenty of pores with diameter of about 5-10μm were exist on the surface of active carbon. The adsorption process with toluene was performed over the modified active carbon to investigate the adsorptive efficiency. For comparison, other porous materials were also performed the adsorption experiment. The results showed that active carbon modified by hydrochloric acid has high adsorptive efficiency for toluene. The results proved that adsorptive performance of active carbon increased with the hydrochloric acid concentration.

Application of Molybdenum ZSM-5 for Catalytic Oxidation of Wastewater Containing Methylene Blue

2020 ◽  
Vol 7 (3) ◽  
pp. 256-264
Author(s):  
Debarpita Ghosal ◽  
D. Kissan Achary ◽  
Sandil Nayak ◽  
Bikash Kumar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Methylene Blue ◽  
Catalytic Oxidation ◽  
Surface Area ◽  
Crystalline Structure ◽  
Energy Dispersive ◽  
X Ray ◽  
Metal Impregnation ◽  
Scanning Electron

Background:: A vital section of water pollution mostly comes from the dying industry, which contaminates the water bodies by discharging the effluents into them. Naturally, it is carcinogenic as it contains harmful chemicals and minerals. To prevent this , many researchers have studied the issue and came to an inference that Methylene blue should be removed from wastewater. Many researchers and scientists proposed that Zeolite with little modifications could be one of the feasible options for catalytic oxidation of dyes in wastewater. Our focus is mostly based on Molybdenum impregnated H-ZSM-5 to catalytically oxidize methylene blue present in wastewater. Objective:: This method examined the Catalytic oxidation of wastewater containing Methylene Blue by the application of Mo-ZSM-5. Methods:: Raw H-ZSM-5 was activated and impregnated with previously prepared MoCl5 and allowed to dry and calcine at the required temperature. The product was characterized by Scanning Electron Microscopy, Energy Dispersive X-ray Spectroscopy and BET Surface Area Analyser methods. Catalytic oxidation reactions were carried out at room temperature using hydrogen peroxide as oxidant. The effect of each parameter was investigated vividly. Results:: From the Energy Dispersive X-ray Spectroscopy method, it was observed that the percentage for Molybdenum over H-ZSM-5 was 9%. Surface area analysis suggested that the value for the surface area of unimpregnated H-ZSM-5 was 511 m2/g for 5% impregnation and 307 m2/g for 10% metal impregnation. A sharp decrease in the surface area was observed. Scanning Electron Microscopy images depict that the crystalline structure of raw H-ZSM-5 would not be damaged due to metal impregnation. Its shape and size were unaltered. In the images, the porous surface was observed. Conclusion:: Zeolites are an important catalyst in active phases for acidic/basic/redox catalysed reactions. Its activity and selectivity affected by the crystalline structure as well as morphological properties. Molybdenum impregnated H-ZSM-5 catalyst is best popularly known for its shape selectivity property. It promotes faster decomposition of H2O2 to non-reactive O2, which shows poor oxidation activity.

scholarly journals Biomass Wastes as Precursor For The Synthesis of Carbon Nanoparticle

2021 ◽  
Vol 945 (1) ◽  
pp. 012035
Author(s):  
Brenda Lim Ai Lian ◽  
Steven Lim ◽  
Pang Ling Yean ◽  
Siew Hoong Shuit ◽  
Huei Wong Kam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Energy Storage ◽  
Oil Palm ◽  
Electrode Material ◽  
Impregnation Method ◽  
Carbon Nanoparticle ◽  
Hierarchical Porous ◽  
Bet Analysis ◽  
Scanning Electron

Abstract The development of technology and the growth in human population had resulted in a surge in energy demand and biomass waste production. Metal-air battery (MAB) is a potential energy storage technology with high theoretical energy density and safety. However, the conventional air cathode material synthesized from carbon nanotube (CNT) is rather costly. In this study, several biomass wastes such as oil palm empty fruit bunch (OPEFB), garlic peel (GP) and oil palm frond (OPF) were investigated to identify a suitable greener and efficient precursor to syntheelesize carbon nanoparticle as air electrode material for MAB. The carbon materials were synthesized through carbonization of precursor at different temperatures of 450 °C, 600 °C, and 700 °C before activation with potassium hydroxide (KOH) through wet impregnation method. The materials synthesized were evaluated based on its chemical and physical properties through characterization using thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy with energy dispersion x-ray (SEM-EDX), field emission scanning electron microscopy (FESEM) and Brunauer-Emmet-Teller (BET) analysis. Based on the experiments conducted, it was found that the suitable precursor was the OPF as it displayed a better tunability for enhanced electrical conductivity as it was able to achieve smaller sized particles with higher specific surface area of 548.26 m2/g and hierarchical porous structure at 700 °C compared to OPEFB and GP. This study proved that OPF could be a promising alternative to CNT as an electrode material which is more sustainable and cost efficient for energy storage application such as MAB.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

Microbulldozing and Microchiseling

1969 ◽  
Vol 27 ◽  
pp. 134-135
Author(s):  
J.N. Ramsey ◽  
D.P. Cameron ◽  
F.W. Schneider
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Material Handling ◽  
Microprobe Analysis ◽  
Foreign Matter ◽  
Specific Location ◽  
Potential Problems ◽  
Knoop Indenter ◽  
Scanning Electron ◽  
Microhardness Tester

As computer components become smaller the analytical methods used to examine them and the material handling techniques must become more sensitive, and more sophisticated. We have used microbulldozing and microchiseling in conjunction with scanning electron microscopy, replica electron microscopy, and microprobe analysis for studying actual and potential problems with developmental and pilot line devices. Foreign matter, corrosion, etc, in specific locations are mechanically loosened from their substrates and removed by “extraction replication,” and examined in the appropriate instrument. The mechanical loosening is done in a controlled manner by using a microhardness tester—we use the attachment designed for our Reichert metallograph. The working tool is a pyramid shaped diamond (a Knoop indenter) which can be pushed into the specimen with a controlled pressure and in a specific location.

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