About a case of macro-FSH analytical interference

2021 ◽  
Vol 79 (4) ◽  
pp. 353-355
Author(s):  
Claire Guibet ◽  
Cindy Lauro ◽  
Virginie Grouthier ◽  
Jean-Benoît Corcuff ◽  
Alizée Violin ◽  
...  
Keyword(s):  
Analytical Interference

scholarly journals Falsely diagnosed thyrotoxicosis caused by anti-streptavidin antibodies and pre-wash procedures

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Takuya Ishikawa ◽  
Hiroyuki Sakai ◽  
Tokutaro Itaya ◽  
Suwanai Hirotsugu ◽  
Jumpei Shikuma ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Magnetic Particle ◽  
Thyroid Function Tests ◽  
Case Presentation ◽  
Analytical Interference ◽  
Function Tests ◽  
Inappropriate Treatment ◽  
Published Report ◽  
Old Japanese ◽  
Polyethylene Glycol Precipitation

Abstract Background Anti-streptavidin antibodies are causal determinants of analytical interference during Thyroid function tests, and numerous reports have detailed such interference, with anti-streptavidin antibodies attracting attention. Case presentation We conducted a straightforward investigation of interference due to anti-streptavidin antibodies, with a case of a 60-year-old Japanese man who consulted our department for inconsistencies between his clinical course and Thyroid function tests. Experiments were conducted using Cobas8000 e602, which employs assay procedures with pre-wash to evaluate FT4 and FT3 levels. Conclusions To our knowledge, this is the first published report to clearly investigate such interferences using a combination of polyethylene glycol precipitation, heterophilic blocking tube precipitation, streptavidin-coated magnetic particle precipitation, and different instruments with or without pre-wash. Clinicians should consider that interferences caused by anti-streptavidin antibodies could lead to a misdiagnosis of thyrotoxicosis. Moreover, discussions between laboratory specialists, clinicians, and manufacturers are required to identify interferences and avoid unnecessary examinations and inappropriate treatment.

Analytical interference testing analysis of multi-segment horizontal well

2018 ◽  
Vol 171 ◽  
pp. 919-927 ◽  
Author(s):  
Youwei He ◽  
Shiqing Cheng ◽  
Jiazheng Qin ◽  
Zhi Chai ◽  
Yang Wang ◽  
...  
Keyword(s):  
Horizontal Well ◽  
Analytical Interference

scholarly journals Analytical Interference Leading to a Diagnosis of Lymphoproliferative Disorder

2017 ◽  
Vol 30 (1) ◽  
pp. 55-57
Author(s):  
Irena Kurfürstová ◽  
Tomáš Šálek ◽  
Eva Kadlčková ◽  
Marek Pšenčík
Keyword(s):  
Lymphoproliferative Disorder ◽  
Analytical Interference

scholarly journals Pregnancy, malignancy or mother nature? Persistence of high hCG levels in a perimenopausal woman

2019 ◽  
Vol 12 (1) ◽  
pp. bcr-2018-227203
Author(s):  
Ayşe Y Demir ◽  
Ruben EA Musson ◽  
Willem A Schöls ◽  
Jitze M Duk
Keyword(s):  
Laboratory Tests ◽  
Transvaginal Ultrasound ◽  
Hormone Replacement ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Repeated Testing ◽  
Analytical Interference ◽  
Enormous Amount ◽  
Suppression Test ◽  
Menopausal State

Despite the fact that a small percentage of peri en postmenopausal women have mild elevations in human chorionic gonadotrophin (hCG) concentrations (<14 IU/L) besides high levels of gonadotrophins, a considerable number of clinicians are not aware of this phenomenon. We report a case of a 53-year-old woman with an unusually high hCG concentration (>40 IU/L) given her menopausal state. Although a pregnancy or a malignancy was unlikely on the basis of stable hCG levels, elevated gonadotrophins and a negative transvaginal ultrasound, her physicians were uncertain and chose an expectant approach by repeated testing. Ultimately, after consulting the laboratory, analytical interference was ruled out and pituitary origin of unusual high hCG level could be confirmed after conduction of a suppression test by oestrogen–progesterone hormone replacement therapy. Until that time, the patient had undergone a vast amount of laboratory tests and gynaecology consultations, resulting in an enormous amount of confusion, anxiety and overdiagnosis.

scholarly journals Erroneously High Lactate: A Guide to Diagnosing Ethylene Glycol Poisoning

2019 ◽  
Vol 4 (2) ◽  
pp. 1-3
Author(s):  
Mayanka Kamboj ◽  
Harini Bejjanki ◽  
Saraswathi Gopal ◽  
Rupam Ruchi
Keyword(s):  
Lactic Acid ◽  
Lactic Acidosis ◽  
Ethylene Glycol ◽  
Critically Ill Patients ◽  
Poor Prognosis ◽  
Acid Level ◽  
Lactic Acid Level ◽  
Analytical Interference ◽  
Ethylene Glycol Poisoning ◽  
High Lactate

A high lactic acid level in critically ill patients is a marker of poor prognosis. However, lactic acidosis in ethylene glycol (EG) poisoning should be interpreted cautiously as analytical interference is observed with EG metabolites.

Interference of anti-streptavidin antibodies in immunoassays: a very rare phenomenon or a more common finding?

2020 ◽  
Vol 58 (10) ◽  
pp. 1673-1680
Author(s):  
Nick Verougstraete ◽  
Mario Berth ◽  
Mario Vaneechoutte ◽  
Joris Delanghe ◽  
Nico Callewaert
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Thyroid Stimulating Hormone ◽  
Common Finding ◽  
Analytical Interference ◽  
Random Samples ◽  
Sds Page ◽  
Before And After ◽  
Multiple Samples ◽  
The Relationship

AbstractBackgroundAnti-streptavidin antibodies (ASA) may cause analytical interference on certain immunoassay platforms. Streptavidin is purified from the non-pathogenic Streptomyces avidinii soil bacterium. In contrast to interference with biotin, ASA interference is supposed to be much rarer. In-depth studies on this topic are lacking. Therefore, we carried out an analysis toward the prevalence and the possible underlying cause of this interference.MethodsAnti-streptavidin (AS)-immunoglobulin G (IgG) and AS-IgM concentrations were determined on multiple samples from two patients with ASA interference and on 500 random samples. On a subset of 100 samples, thyroid-stimulating hormone (TSH) was measured on a Cobas analyzer before and after performing a neutralization protocol which removes ASA. The relationship between the ratio of TSH after neutralization/TSH before neutralization and the ASA concentration was evaluated. Subsequently, an extract of S. avidinii colonies was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting.ResultsA positive correlation between AS-IgM concentrations and TSH ratio was obtained. Eight samples out of 500 exceeded the calculated AS-IgM cut-off value. In comparison to the AS-IgM concentrations in the population, titers from the two described cases clearly stood out. The isolated cases represent the end of a broader spectrum as there is a continuum of AS-IgM reactivity in the general population. We could not observe any differences in the immunoblot patterns between the cases and controls, which may indicate the general presence of ASA in the population.ConclusionsInterference due to ASA is more prevalent than initially thought and is caused by IgM antibodies.

An X-ray diffraction study of filters used for atmospheric aerosol sampling

1996 ◽  
Vol 11 (3) ◽  
pp. 230-234 ◽  
Author(s):  
M. D. Santos ◽  
J. R. Matos ◽  
L. R. F. Carvalho ◽  
L. M. Sant’Agostino ◽  
M. Korn
Keyword(s):  
Atmospheric Particulate Matter ◽  
Aerosol Sampling ◽  
X Ray Diffraction ◽  
Analytical Strategy ◽  
X Ray ◽  
Analytical Interference ◽  
Diffraction Patterns ◽  
Artifact Formation ◽  
Thermally Treated

Characterization of some crystalline species present in atmospheric particulate matter can be investigated by an X-ray diffractometric technique. According to the analytical strategy, filtering media suitable for collecting airborne particles must be selected. In order to recognize the X-ray diffraction patterns and consequently the inherent analytical interference of filtering media, a systematic X-ray diffraction evaluation of several substrates was performed. Although artifact formation during ambient sampling can occur on quartz and glass fiber filters, these filters were also included in the diffractometric characterization. In this work, commercial filters were thermally treated and submitted to X-ray diffraction scanning. Results have shown pronounced variations in the diffractometric profiles of each thermally treated substrate. The selection criteria for choosing the filtering media was established by considering their chemical and physical properties and also the crystalline species to be collected on them.

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