scholarly journals Detection of Peach latent mosaic viroid by PCR

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 249-251
Author(s):  
P. Ryšánek ◽  
M. Zouhar ◽  
M. Hassan

Peach latent mosaic viroid (PLMVd) is widespread in peach all over the world. It has never been reported from the Czech Republic. That is why we adapted specific and sensitive method for its detection, PCR, to be able to prove its possible occurrence and for certification purposes. Primers PLMVdR, PLMVdF1 and PLMVdF2 were designed on the basis of published RNA sequences. Products of amplification are 208 and 114 bp long for PLMVdF1 and PLMVdF2, respectively. Four PLMVd isolates from Dr Di Serio (CNR Bari) were used as standards. Potato spindle tuber viroid and Hop latent viroid infected plant material and also healthy material were used to check detection specifity. Both RNA extraction from plant material and PCR were optimalized so that this method of PLMVd detection can also be used for certification purposes.

2020 ◽  
Vol 168 ◽  
pp. 00007
Author(s):  
Jan Fiala ◽  
Miroslav Lapka ◽  
Jan Fiala jun ◽  
Milan Mikolas

First technological process, which ceramic producers of ceramic products use for their future ceramic product is mining process. Mining methods, Transport methods and processing methods are basic technological processes. The process of drying, firing and shaping of brick products are specific. Babylonian gardens, Hagia Sofia, are one of the most beautiful churches that has ever been built. The great Wall of China, medieval castle Malbork from northern Poland not far from Gdansk`s bay which resembles small town, Skyscraper Chrysler Building in New York are the outstanding brick creations in the world. All of these buildings have one thing in common and that is material from which they were built. The material is brick, one of the simplest, the most beautiful, the most universal and the oldest building product which is known for moreover than 10 000 years [1]. Brick association of Czech and Moravia, nowadays unites 6 regular members with 19 plants where are created fired building materials. Furthermore it unites 7 associated members who ensure various services for brickmakers and 7 honorary members. Producers, members of brick association represent 92 % of Mason material capacity in the Czech Republic and 100 % of fired roofing tile capacity.


2013 ◽  
Vol 59 (No. 4) ◽  
pp. 183-193 ◽  
Author(s):  
L. Smutka ◽  
J. Burianová

World trade underwent a significant shock within the recent years, which caused a decline in the world economy primarily in the year 2009. Within the following years (2010 and 2011), the high rate of growth from the years preceding the crisis could not be restored. The crisis had an impact on all segments of the merchandise trade, whereby the trade in agricultural and food products was affected the least by the crisis. In the case of the Czech Republic, the crisis of the global and national economy was reflected in the case of agricultural trade primarily by the way of a decline in the rate of the growth of export, which was very high in the period prior to the crisis. As far as the territorial structure and commodity structure of agricultural trade is concerned, their development in the years 2008–2011 was not affected in any largely significant manner. In relation to the main objective of this article, which was to identify the effects of the crisis on the competitiveness of Czech agricultural trade, it may be stated that the crisis itself did not worsen the competitiveness of agricultural trade in any significant manner.  


Author(s):  
M. Hejl ◽  
Martin Mohapl ◽  
Lukáš Bříza

This article wants to introduce modular green roof systems and their usage in the world as well as in the Czech Republic, because green roofs are now used more than they have been constructed in any era in the history. Their construction is almost same as it was in previous centuries, so the obvious solution for their improvement is in the construction of themselves. Especially in the improvement of their construction time which should be lower with usage of modular system than at classical layer system of the green roofs. This research also wants to speak about construction system for individual types of green roof. Where the purpose of this article is to set optimal solution for modular green roof panel which will be economical and environmentally friendly.


2011 ◽  
Vol 61 (3) ◽  
pp. 489-497 ◽  
Author(s):  
J. Adriko ◽  
V. Aritua ◽  
C. N. Mortensen ◽  
W. K. Tushemereirwe ◽  
J. Kubiriba ◽  
...  

Author(s):  
Libor Žídek

Development of international trade is (in our view) one of the greatest achievements of the transformation process in Eastern Europe. In the paper we illustrate this achievement by the example of the Czech Republic. We conclude that the economy has enormously changed from closed one with backward production and orientation towards the Eastern markets to one that is among the most open economies in the world, with nearly entire orientation towards the developed markets and with relatively advanced production. This development was partly a result of quick (and in our view brave) liberalization at the beginning of the transformation process. At the same time, we have to conclude that the level of dependency has enormously increased in the course of the respective period and the Czech economy is now highly dependent on the economic development of the main trading partners – foremost the other European countries. This dependency is largely positive but can bring negative aspects in the periods of economic decline.


Ergo ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 3-11
Author(s):  
Zdeněk Kučera ◽  
Tomáš Vondrák

Abstract The Czech Republic is at the forefront of the EU in the contribution of the manufacturing to the total gross value added. The Czech industry contributes significantly to the positive balance of trade of the country. The overall international competitiveness of the Czech Republic will depend upon the innovation performance of the manufacturing sector and its competitiveness on the foreign markets. A necessary condition for extending the share on the foreign markets is the effective harnessing of the national R&D capacity and a strong qualified local workforce. This contribution uses a bibliometric and a patent analysis for the assessment of the R&D carried out by the Czech higher education institutions (HEIs) oriented towards the engineering fields, and selected more general universities, and compares them with similar HEIs in a selected group of developed countries. The analysis indicates that the Czech publication activity in the field of engineering grows. The Czech publications in engineering are cited below the world average which indicates that their impact (and to some extent the quality of the underlying R&D) lags behind the world. The patent activity of the Czech HEIs is on the international scale rather weak. The patenting activity of the Czech HEIs strongly grows since the middle of the last decade. This may by a side effect of the new evaluation methodology which takes into account also the patents. The number of patents registered with the Czech Industrial Property Office increases but on the other hand the international patenting activities tend to stagnate. This gives rise to questions about the real value of the produced patents from the point of their importance for the export competitiveness of the Czech industry.


Author(s):  
Олександр Володимирович Києвич

Nowadays, when we still see the impact of COVID not only in the Czech Republic, but all over the world, when the value of money is constantly decreasing due to inflation and negative trends in the economy, people usually try to save their savings where they are confident that they will not lose value. The purpose of the article is to characterize the policy of the Czech National Bank in relation to the real estate market. Research hypothesis. The population of the Czech Republic now perceives housing as a safe haven and protects their savings by buying real estate. That is why, according to practicing economists, the great interest of Czechs in investing in real estate will continue in the coming years. Presentation of the main material. Wealthy people in the Czech Republic are now investing their money in apartments to protect their savings from inflation, which was largely fueled by covid restrictions. Rising inflation and volatility in world currencies is a serious blow to those who keep their savings in cash, so people want to own any asset that has any hope of going up. Originality and practical significance of the research. It has been proven that overheated markets sometimes collapse with dire consequences for a country's economy. And this is the responsibility of the regulators, who must anticipate and prevent such trends in the markets. Conclusions and prospects for further research. The current situation with the pandemic has not affected the real estate market, which is perhaps surprising. The population of the Czech Republic now perceives housing as a safe haven and protects their savings by buying real estate. The main task today of all financial market regulators, not only in the Czech Republic, but all over the world, is and will be the task of preventing a sharp collapse of the formed bubbles, including the real estate market.


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