scholarly journals Evaluation of the glutathione concentration in whole blood of dairy Holstein cows

Author(s):  
A Necasova ◽  
A Pechova ◽  
R Bodor ◽  
M Masar

The objective of this study was to evaluate the concentrations of the total (T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in the blood of dairy cows, assess the relationships of the phase and number of lactation, milk production, body condition score (BCS) and selected biochemical parameters on its concentrations. We analysed 79 samples of whole blood from dairy Holstein cows. The concentration of glutathione was assessed by the spectrophotometric enzymatic method. The whole dataset shows the mean concentration of T-GSH, GSSG and GSH of 803 ± 22 µmol/l, 23 ± 2.5 µmol/l and 757 ± 27 µmol/l, respectively. The GSH/GSSG ratio was 95 ± 17. The phase of the lactation had a significant impact on the levels of T-GSH and GSH, but the number of lactation and BCS had no effect. Dry cows had higher levels of T-GSH (938 ± 44 µmol/l) than the fresh (713 ± 46 µmol/l) and peak lactation (785 ± 45 µmol/l) cows. The fresh cows had significantly lower concentrations of GSH (618 ± 44 µmol/l) than the peak lactation (719 ± 46 µmol/l) and dry cows (827 ± 43 µmol/l). On the basis of a regression analysis, blood glutathione was affected mainly by the liver function and energy metabolism. Glutathione as the marker of oxidation stress seems to be a promising tool in monitoring the health and welfare of the herd, yet intensive research in this field remains necessary.

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1674
Author(s):  
Ilona Strączek ◽  
Krzysztof Młynek ◽  
Agata Danielewicz

A significant factor in improving the performance of dairy cows is their physiological ability to correct a negative energy balance (NEB). This study, using Simmental (SIM) and Holstein-Friesian (HF) cows, aimed to assess changes in NEB (non-esterified fatty acid; body condition score; and C16:0, C18:0, and C18:1) and its effect on the metabolic efficiency of the liver (β-hydroxybutyrate and urea). The effects of NEB on daily yield, production at peak lactation and its duration, and changes in selected milk components were assessed during complete lactation. Up to peak lactation, the loss of the body condition score was similar in both breeds. Subsequently, SIM cows more efficiently restored their BCS. HF cows reached peak lactation faster and with a higher milk yield, but they were less able to correct NEB. During lactation, their non-esterified fatty acid, β-hydroxybutyrate, C16:0, C18:0, C18:1, and urea levels were persistently higher, which may indicate less efficient liver function during NEB. The dynamics of NEB were linked to levels of leptin, which has anorectic effects. Its content was usually higher in HF cows and during intensive lactogenesis. An effective response to NEB may be exploited to improve the production and nutritional properties of milk. In the long term, it may extend dairy cows’ productive life and increase lifetime yield.


2011 ◽  
Vol 24 (5) ◽  
pp. 610-615 ◽  
Author(s):  
Takeshi Yamazaki ◽  
Hisato Takeda ◽  
Akiko Nishiura ◽  
Youji Sasai ◽  
Naoko Sugawara ◽  
...  

2009 ◽  
Vol 49 (12) ◽  
pp. 1086 ◽  
Author(s):  
A. K. Esmailizadeh ◽  
O. Dayani ◽  
M. S. Mokhtari

The objective of this study was to investigate to what extent fertility and lambing season of fat-tailed ewes raised under an extensive production system are related to liveweight, body condition and changes around mating. Ewe liveweight and body condition score (BSC) were recorded in June, July and August over a period of 3 years (1999–2001) in 11 flocks (eight flocks of Kurdi breed and three flocks of Kurdi × Sanjabi crossbred). Both Kurdi and Sanjabi are native fat-tailed breeds in western Iran. In total, 3278 lambing records relating to 1592 ewes and data on mating date, liveweight and BCS of 1930 ewes (3975 records) were used for statistical analyses. The statistical model for bodyweight and body condition data included fixed effects of breed group, flock nested within breed group, year, the future lambing status following summer breeding (barren, autumn lambing and winter lambing), ewe age and all two-way interactions. Breed group had a significant effect on ewe liveweight (P < 0.01). However, there was no significant difference between BCS of the two breed groups around mating. Ewe liveweights measured in June, July and August had significant effects on fertility and lambing season (P < 0.01). Ewes that produced lambs were generally heavier at mating than barren ewes (P < 0.01). Autumn-lambing ewes were heavier than winter-lambing ewes in June and July (P < 0.01). Effects of changes in liveweight on fertility and lambing season were significant (P < 0.01). The effects of BCS in June, July and August (P < 0.01) and changes in BCS during the mating period (P < 0.05) on fertility and lambing season were also significant. The mean BCS (in June and July) of autumn-lambing ewes was greater than that of the winter-lambing and barren ewes (P < 0.05). The proportion of autumn-lambing ewes significantly increased as BCS in July increased, whereas the proportion of barren ewes decreased (P < 0.01). Fertility was associated with age so that as the age of the ewes increased from 2 to 7 years, the proportion of barren ewes significantly decreased from 29 to 5% (P < 0.01). There was a relatively high proportion of very thin ewes of 2 and 3 years of age. These findings imply that low body condition is a particular problem in younger ewes suggesting the importance of giving special nutritional treatment to younger ewes to reduce the proportion of barren ewes.


1994 ◽  
Vol 45 (4) ◽  
pp. 795 ◽  
Author(s):  
H Hearnshaw ◽  
PF Arthur ◽  
R Barlow ◽  
PJ Kohun ◽  
RE Darnell

Post-weaning growth and body condition, puberty and pelvic size of 197 heifers comprising straightbred Hereford (HxH) and Brahman (BxB), first-cross (BxH) and back-cross (HxBH and BxBH) heifers were evaluated. The heifers were born over a 3 year period, and grazed improved and semi-improved pastures following weaning at Grafton, New South Wales. Prior to weaning, heifers had been reared by dams on three pasture systems (high, medium and low quality pastures). Heifers from low quality pre-weaning pasture had higher (P < 0.05) post-weaning liveweight gain than those from high and medium quality pastures. BxH heifers gained 71 g/day more (P < 0.05) than the mean gain of their contemporaries of the other genotypes, whose gains were similar, from weaning to either 26 or to 30 months of age. Liveweight at all ages was influenced by genotype x pre-weaning pasture system interaction. At 30 months of age, BxH heifers from high and medium pre-weaning pastures were the heaviest. At the same age, but from low quality pre-weaning pasture, heifers with crossbred dams (HxBH and BxBH) were the heaviest. Wither height depended significantly (P < 0.05) on the proportion of Bos indicus genes, increasing from 113.8 cm in the HxH heifers to 124.4 cm in the BxB heifers at 30 months of age. BxH heifers had a higher (P < 0.05) body condition score than their contemporaries of the other genotypes, which were in similar condition, at 26 and 30 months of age. On average (across pre-weaning pasture system), 9% of BxB heifers had reached puberty by 22 months of age compared to 62, 95, 82 and 64% (s.e.= 9) for HxH, HxBH, BxH and BxBH heifers respectively. No significant genotype differences were obtained in the height, width and size of the pelvic opening of the heifers, measured just prior to the beginning of the mating season at 26 months of age.


Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1408
Author(s):  
Melissa Dorn ◽  
Anja Becher-Deichsel ◽  
Barbara Bockstahler ◽  
Christian Peham ◽  
Gilles Dupré

Laparoscopy is a growing field in veterinary medicine, although guidelines are lacking. The objective of this study was to evaluate the pressure–volume curve during capnoperitoneum in cats. A total of 59 female cats were scheduled for routine laparoscopy. Pressure and volume data were recorded and processed, and the yield point of the curve was calculated using a method based on a capacitor discharging function. For the remaining 40 cats, a linear-like pressure–volume curve was observed until a yield point with a mean cutoff pressure (COP) of 6.44 ± 1.7 mmHg (SD) (range, 2.72–13.00 mmHg) and a mean cutoff volume (COV) of 387 ± 144.35 mL (SD) (range, 178.84–968.43 mL) was reached. The mean mL/kg CO2 value in cats was 208 ± 34.69 mL/kg (range, 100.00–288.46 mL/kg). The COV correlated with COP and body weight but not with body condition score (BCS). COP correlated only with the COV. This study suggests that feline patients have a pressure–volume curve similar to that of canine patients, and the same pressure limit recommendations can be used for both species. After a yield point of 6.44 mmHg is reached, the increment in volume decreases exponentially as the intra-abdominal pressure (IAP) increases.


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