scholarly journals Antioxidant and antimicrobial potential of artichoke (Cynara scolymus L.) extract in beef patties

2018 ◽  
Vol 36 (No. 2) ◽  
pp. 154-162 ◽  
Author(s):  
Ergezer Haluk ◽  
Kaya Halil Ibrahim ◽  
Şimşek Ömer
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Coliform Bacteria ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antimicrobial Potential ◽  
Beef Patties ◽  
Antioxidant And Antimicrobial Activity

The antioxidant and antimicrobial potential of artichoke extract (AE) in raw beef patties (RBPs) was evaluated during the storage. The RBPs were prepared with the addition of 500 and 1000 ppm AE. Also, Escherichia coli ATCC25922 and Listeria monocytogenes ATCC19118 were inoculated to each RBP to follow the antimicrobial activity. An evaluation of the instrumental colour, pH, total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, TBARS and microbiological properties was carried out during storage. The evaluation revealed that the a* value decreased significantly, so that the reduction level of the AE500 and AE1000 samples during storage was  35, 57, and 56%, respectively, in the control. AE addition slightly decreased the pH of RBPs, which varied between 5.72 and 5.96. The TPC and DPPH values of samples with added 500 and 1000 ppm AE were 2 to 3-fold and 3 to 7-fold higher than in the control, respectively. Also, the TBARS values were determined as 43 and 54% lower than in the control at the end of storage when 500 and 1000 ppm AE were used. The AE in RBP inhibited the viability of total aerobic psychrophilic bacteria, coliform bacteria and yeast-mould in a concentration-dependent manner. AE prevented the growth of E. coli ATCC25922 and L. monocytogenes ATCC19118 inoculated to the RBPs. In conclusion, 1000 ppm AE was sufficient for antioxidant and antimicrobial activity in RBP. To our knowledge, this is the first study that presents the antioxidant and antimicrobial activity of AE used in a food model system.

Antioxidant Activities of Xiao-Yan-Hua-Jie-San and Identification of the Active Components

2013 ◽  
Vol 781-784 ◽  
pp. 1020-1026
Author(s):  
Yu Ping Li ◽  
Li Hua Yao ◽  
Guang Jie Wu ◽  
Fu Quan Jiang ◽  
Xiang Yuan Xiong ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Model Systems ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner

Xiao-Yan-Hua-Jie-San (XYHJS), a traditional Chinese prescription, is used as a medication recipe to clinically treat inflammation and hepatitis. In previous study, we reported the hepatoprotective effects of XYHJS by increasing an antioxidant enzyme activity in mice. In the present study, the main bioactive components of XYHJS and their antioxidant activity were further investigated by using different model systems in vitro. The total phenolics content in the extract of XYHJS was determined by the Folin-Ciocalteu method. Analysis of the major phenolic compounds in the extract of XYHYS was carried out by high-performance liquid chromatography (HPLC) and thin layer chromatographic (TLC) method. The total phenolic content of the extract was 2.84 ± 0.06 mg gallic acid equivalent (GAE)/g extract powder. The phenolic acid in XYHJS was found to be gallic acid (GA). The content of GA was 2.80 mg/ml by HPLC (n=5, RSD=1.26%). Furthermore, the antioxidant activity of XYHJS extract was determined by 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity and hydroxyl free radicals (·OH) scavenging activity assay. The radical scavenging activity of XYHJS increased significantly in a concentration-dependent manner. At a concentration of 2.5 mg/ml, the DPPH and ·OH scavenging activity was 89.94% and 91.53%, respectively. It can be concluded that the content of GA in XYHJS is very high and it is the main contributor to the antioxidant activity of XYHJS. Our study indicates that XYHJS prescription could be considered to be an effective agent in the prevention of various liver diseases associated with oxidative stress.

Bioaccessibility, antioxidant and antibacterial potentials of phenolic compounds ultrasonically extracted from acerola Malpighiaglabra DC coproduct

2021 ◽  
Vol 17 ◽  
Author(s):  
Jorgiane da Silva Severino Lima ◽  
Larissa Morais Ribeiro da Silva ◽  
Thatyane Vidal Fonteles ◽  
EvâniaAltina Teixeira de Figueiredo ◽  
Ana Erbênia Pereira Mendes ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Green Technology ◽  
Total Phenolic ◽  
Power Intensity ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activity

Background: This study assessed the effects of solvent proportion, time, and power intensity of ultrasound processing on the extraction,bioaccessibility, antioxidant and antimicrobial activity of phenolic compounds (PC) from acerola (Malpighia glabra DC) coproduct (ACP). Methods: The ultrasound process with water as a solvent in a ratio of 1:2, power intensity of 75 W/cm2 during 10 min was selected as the optimal condition to provide higher extraction of the total phenolic compounds (4126 mg gallic acid.100 g-1 with total antioxidant activity of 98.62 μMTrolox.g-1). Power intensity and solid: solvent proportion were the parameters that increased the total PC quantification. The main phenolic compounds from the ACP tentatively identified by UPLC-ESI-QTOF-MS/MS were rutin, luteolin, and quercetin. Ultrasound extraction also improved antimicrobial activity against Listeria monocytogenes and Staphylococcus aureus(40 mg.L -1). Results: These results indicate that antioxidant and antimicrobial activities in the ACP phenolic extracts are quite potent and implicate the presence of compounds with potent free-radical-scavenging activity. Conclusion: It is possible to extract phenolic compounds from acerola coproducts using sustainable “green” technology, only using water as a solvent and ultrasound processing.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

scholarly journals Radical scavenging activity of crude polysaccharides from Camellia sinensis

2011 ◽  
Vol 63 (3) ◽  
pp. 717-721
Author(s):  
Fan Yang ◽  
Zhiwei Yang ◽  
Jianbo Xiao
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Camellia Sinensis ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Molecular Weights

A preparation of crude polysaccharides (TPS) was isolated from Camellia sinensis by precipitation and ultrafiltration. TPS1, TPS2, and TPS3 had molecular weights of 240, 21.4, and 2.46 kDa, respectively. The radical scavenging activities of TPS were evaluated by DPPH free radical, hydroxyl radical and superoxide radical scavenging. These results revealed that TPS exhibited strong radical scavenging activity in a concentration-dependent manner. TPS3 with lowest molecular weight showed a higher radical scavenging activity.

scholarly journals Antimicrobial Activity and Ellagitannins from Terminalia Ferdinandiana

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 81
Author(s):  
Akter ◽  
Hong ◽  
Netzel ◽  
Tinggi ◽  
Osborne ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Free Radical ◽  
Morphological Changes ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Agar Disc ◽  
Kakadu Plum ◽  
Terminalia Ferdinandiana

Extracts were prepared from Terminalia ferdinandiana (Kakadu plum) fruits, leaves, seedcoats, and barks using accelerated solvent extraction with methanol, ethanol, water, acetone, and hexane. Antioxidant activity was assessed using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and by determining the total phenolic content (TPC). Methanol fruit extracts had the strongest free radical scavenging activity and the highest TPC (12 g/100 g DW). The antimicrobial activity of all extracts (except hexane) was analysed by agar disc diffusion. Fruits and leaves showed larger inhibition zones against foodborne bacteria Listeria monocytogenes, Bacillus cereus, methicillin resistant Staphylococcus aureus, and clinical isolates of Pseudomonas aeruginosa compared to seedcoats and barks. The minimum inhibitory and minimum bactericidal concentration of the extracts ranged from 1.0 to 3.0 mg/mL against the tested microorganisms. Scanning electron microscopy images of bacteria treated with the extracts showed morphological changes consistent with cell death. A new UHPLC-MS/MS method for the determination of punicalagin and castalagin, bioactive ellagitannins, was developed and validated. Punicalagin contents in the fruits and leaves were 74 and 49 mg/100 g DW whilst castalagin contents were 1.1 and 0.8 mg/100 g DW. To the best of our knowledge, this is the first report about punicalagin and castalagin concentrations in Kakadu plum fruits and leaves. These initial findings are very promising in regards to the potential use of Kakadu plum fruit and leaf extracts as natural preservatives in the food industry.

scholarly journals In Vitro Antioxidant Activity of Dibenz[b,f]azepine and its Analogues

2008 ◽  
Vol 5 (s2) ◽  
pp. 1123-1132 ◽  
Author(s):  
H. Vijay Kumar ◽  
C. R. Gnanendra ◽  
Nagaraja Naik ◽  
D. Channe Gowda
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Antioxidant Compounds ◽  
Dependent Manner ◽  
Concentration Dependent Manner

Dibenz[b,f]azepine and its five derivatives bearing different functional groups were synthesized by known methods. The compounds thus synthesized were evaluated for antioxidant potential through different in vitro models such as (DPPH) free radical scavenging activity,ß-carotene-linoleic acid model system, reducing power assay and phosphomolybdenum method. Under our experimental condition among the synthesized compounds dibenz[b,f]azepine (a) and 10-methoxy-5H-dibenz[b,f]azepine (d) exhibited potent antioxidant activity in concentration dependent manner in all the above four methods. Butylated hydroxyl anisole (BHA) and ascorbic acid (AA) were used as the reference antioxidant compounds. The most active compounds like dibenz[b,f]azepine and its methoxy group substituent have shown more promising antioxidant and radical scavengers compared to the standards like BHA and ascorbic acid. It is conceivable from the studies that the tricyclic amines,i.e. dibenz[b, f]azepine and some of its derivatives are effective in their antioxidant activity properties.

scholarly journals DNA Damage Protecting Activity and Free Radical Scavenging Activity of Anthocyanins from Red Sorghum (Sorghum bicolor) Bran

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
P. Suganya Devi ◽  
M. Saravana Kumar ◽  
S. Mohan Das
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Food ◽  
Anthocyanin Content ◽  
Dependent Manner ◽  
Total Anthocyanin ◽  
Methanol Extracts ◽  
Sorghum Bran

There is increasing interest in natural food colorants like carotenoids and anthocyanins with functional properties. Red sorghum bran is known as a rich source for anthocyanins. The anthocyanin contents extracted from red sorghum bran were evaluated by biochemical analysis. Among the three solvent system used, the acidified methanol extract showed a highest anthocyanin content (4.7 mg/g of sorghum bran) followed by methanol (1.95 mg/g) and acetone (1 mg/g). Similarly, the highest total flavonoids (143 mg/g) and total phenolic contents (0.93 mg/g) were obtained in acidified methanol extracts than methanol and acetone extracts. To study the health benefits of anthocyanin from red sorghum bran, the total antioxidant activity was evaluated by biochemical and molecular methods. The highest antioxidant activity was observed in acidified methanol extracts of anthocyanin in dose-dependent manner. The antioxidant activity of the red sorghum bran was directly related to the total anthocyanin found in red sorghum bran.

scholarly journals Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7290
Author(s):  
Sergey Popov ◽  
Vasily Smirnov ◽  
Elizaveta Kvashninova ◽  
Victor Khlopin ◽  
Fedor Vityazev ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Exchange Chromatography ◽  
Enzyme Hydrolysis ◽  
Dependent Manner ◽  
Apple Pectin ◽  
Pectic Polysaccharides ◽  
Concentration Dependent Manner ◽  
Epilobium Angustifolium

The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively. EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17–133 μg/mL, whereas commercial apple pectin scavenged at 0.5–2 mg/mL. The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50—0.050, 0.109 and 1.961 mg/mL), respectively. Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively. Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains. The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.

scholarly journals Antioxidant and Antimicrobial Properties of Moringa oleifera Leaves and Pods Extracts in Pork Meatballs During Cold Storage

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Patcharee Prasajak ◽  
Phanida Renumarn ◽  
Wichien Sriwichai ◽  
Pakkawat Detchewa
Keyword(s):  
Aqueous Extract ◽  
Cold Storage ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Concentration Dependent Manner

Effects of M. oleifera leaves and pods extracts on physicochemical properties, free radical scavenging properties, antimicrobial activities and sensory attributes of pork meatballs were evaluated during cold storage at 4°C for 15 days. The preparation of pork meatballs was divided into eight treatments as control, 0.02% butylated hydroxytoluene (BHT), 0.2% leaves and pods aqueous extract, 0.4% leaves and pods aqueous extract, 0.8% leaves and pods aqueous extract. Aqueous leaves extract showed highest level of total phenolic (67.18 mg GAE/g extract) and flavonoid contents (5.60 mg CE/g extract) compared to those observed in aqueous pods extract as 55.17 mg GAE/g extract and 3.54 mg CE/g extract, respectively. The leaves extract had strongest antioxidant activity against DPPH radicals with IC50 49.85 μg/ml while the pods extract exhibited IC50 99.31 μg/ml. According to pork meatballs analysis, meatballs samples with addition of aqueous leaves extract exerted higher antioxidant activities in a concentration-dependent manner that were performed by higher DPPH scavenging activity and lower TBARs values in comparison with aqueous pods extract. Conversely, M.oleifera pods extract showed highest antibacterial activity against all testedfoodborne bacterial strains including Staphylococcus aureus (TISTR 1466),Bacillus cereus (TISTR 678), Escherichia coli (TISTR 780), Salmonellatyphimurium (ATCC 13311) with lowest MIC (1.56 mg/ml) and MBC (3.13 mg/ml)in agreement with the decrease of total microbial counts as compared to controland BHT samples. The meatballs with pods extract possessed higher sensoryattributes scores than those added with the leaves extract. In conclusion, 0.8%pods extracts effectively retarded lipid oxidation as well as decreased microbialgrowth in pork meatballs during cold storage. However, it was point out thatinferior sensory scores were affected by increasing additional the extract in themeatballs. Therefore, the use of Moringa extracts should be carefully applied inthe meatballs for avoidance of lowering consumer acceptance.

scholarly journals Saussurea lappa Clarke extract exhibits potent antioxidant effect and attenuates neuroinflammatory responses in lipopolysaccharide-stimulated microglial cells

2020 ◽  
Vol 19 (9) ◽  
pp. 1911-1917
Author(s):  
Sung-Gyu Lee ◽  
Hyun Kang
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enzyme Linked Immunosorbent Assay ◽  
No Production ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Saussurea Lappa ◽  
Tnf Α

Purpose: To investigate the antioxidant and anti-neuroinflammatory potential of Saussurea lappa Clarke (SLC-EA) extract in LPS-stimulated BV-2 microglial cells.Methods: Cell viability was measured by using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay while antioxidant activity was evaluated by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Lipopolysaccharide (LPS) was used to stimulate BV-2 microglia. Griess assay was employed to assess nitric oxide (NO) production. iNOS (inducible NO synthase) expression and TNF-α (tumor necrosis factor-alpha) cytokine production were measured by ELISA (enzyme-linked immunosorbent assay) and immuno blot analysis, respectively.Results: Pretreatment of 100 mg/ml of SLC-EA (p < 0.001) was inhibited Nitric Oxide (NO) by 1 ug/ml of LPS-treated murine BV-2 cells. The expression of iNOS and TNF-α were reduced by SLC-EA concentration dependent manner (p < 0.001 at 100 mg/ml). SLC-EA were scavenged 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner with an IC50 value of approximately 51.4 μg/ml.Conclusion: The results indicate that SLC-EA extract exhibits strong antioxidant properties and inhibits excessive pro-inflammatory cytokine due probably to the antioxidant phenolic compounds present in SLC-EA extract. Further work in exploring the in-depth mechanisms of SLC-EA extract in regulating inflammatory signaling pathways in treating neuroinflammatory diseases is necessary. Keywords: Saussurea lappa, Antioxidant, Neuroinflammation, Microglia, TNF-α, iNOS

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