scholarly journals Comparison of organic matter digestibility determined by in vivo and in vitro methods

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 47-53 ◽  
Author(s):  
J. Forejtová ◽  
F. Lád ◽  
J. Třináctý ◽  
M. Richter ◽  
L. Gruber ◽  
...  
Keyword(s):  
Organic Matter ◽  
In Vitro Digestibility ◽  
Regression Equations ◽  
Two Stage ◽  
In Vitro Techniques ◽  
In Vitro Methods ◽  
Organic Matter Digestibility

A total of 36 samples of feed &ndash; 19 samples of hays and 17 samples of silages were used for estimation of in vivo and in vitro digestibility. The organic matter digestibility (OMD) was estimated by in vivo and two in vitro techniques (method of Tilley and Terry and two-stage pepsin-cellulase method (Pepcel)). The regression equations were calculated from the results obtained in the experiment. Tilley and Terry method provided consistent results of OMD estimation for both the groups of roughages: in vivo OMD = 14.7 + 0.782 &times; [Tilley and Terry] (n = 18; R<sup>2</sup> = 0.76); in vivo OMD = 36.3 + 0.513 &times; [Tilley and Terry] (n = 16; R<sup>2</sup> = 0.75) for hays and silages, respectively. Different accuracy was found out in Pepcel method: in vivo OMD = 37.0 + 0.478 &times; [Pepcel] (n = 19; R<sup>2</sup> = 0.49); in vivo OMD = 37.8 + 0.484 &times; [Pepcel] (n = 17; R<sup>2</sup> = 0.87) for hays and silages, respectively. &nbsp; &nbsp;

scholarly journals Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants

2009 ◽  
pp. 79-86 ◽  
Author(s):  
Dragan Palic ◽  
Klaas-Jan Leeuw
Keyword(s):  
Organic Matter ◽  
Prediction Equation ◽  
Gas Production ◽  
Two Stage ◽  
In Vitro Methods ◽  
The Mean ◽  
Organic Matter Digestibility ◽  
Analytical Laboratories

In this study, the organic matter digestibility (OMD) of six complete diets for ruminants has been determined in-vivo in trials with sheep and in-vitro using two-stage Tilley and Terry (T&T) method, gas production (GP) technique and multi-enzyme incubation (EDOM) procedures. The mean OMD values obtained in vivo and using T&T, GP and EDOM techniques were 684, 716, 685 and 710 g OM/kgDM respectively and did not differ significantly (P>0.05). The obtained in vitro results were regressed against determined in-vivo values to derive prediction equations. Using the T&T technique, the prediction equation OMD (in_vivo) = -17.36 + 0.98 x OMD (in_vitro_T&T), (R2 = 0.75; RMSE = 37.59) has been obtained. The equation OMD (in_vivo) = 198.98 + 0.71 x OMD (in_vitro_GP), (R2 = 0.21; RMSE = 66.36) has been derived for Gas production procedure, while the equation OMD (in_vivo) = 102 + 0.82 x OMD (in_vitro_EDOM), (R2 = 0.86; RMSE = 27.30) has been generated for multi-enzyme incubation technique. The results of this study showed that the OMD of complete diets for ruminants can be successfully determined, and in-vivo values predicted, using multi-enzyme incubation procedure, which is important because of the fact that rumen liquor, needed for the in-vitro twostage T&T and GP techniques is not always available to analytical laboratories.

Laboratory analyses of the nutritional value of western Queensland browse feeds

1979 ◽  
Vol 19 (98) ◽  
pp. 344 ◽  
Author(s):  
WJF McDonald ◽  
JH Ternouth
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Crude Fibre ◽  
Dry Matter Digestibility ◽  
Nitrogen Free Extract ◽  
Organic Matter Digestibility

Seventy samples of browse feeds, collected during late autumn and spring of 1971 from 42 western Queensland shrubs and trees, were subjected to laboratory analyses of the proximate fractions and in vitro digestibility. Crude protein, crude fibre and nitrogen-free-extract fractions ranged between 65 and 242, 55 and 353 and 425 and 71 1 g kg-1 dry matter, repectively. The in vitro dry matter digestibility coefficients ranged between 0.22 and 0.86. Comparisons of 15 browse feeds collected in April-May and October indicated a higher crude protein content (P < 0.05) and in vitro digestibility (P .= 0.01) and lower crude fibre (P < 0.01) and ash (P < 0.01 ) content in spring samples for most species. Nine browse feeds of known in vivo dry matter and organic matter digestibility were subjected to in vitro digestibility analysis. Whilst the in vitro and in vivo dry matter digestibilities were similar (0.43 and 0.45, respectively) the mean in vitro organic matter digestibility was substantially lower than the corresponding in vivo digestibility (0.40 and 0.46, respectively).

A comparison of two enzymatic in vitro methods to predict in vivo organic matter digestibility of perennial ryegrass

2015 ◽  
Vol 177 ◽  
pp. 33-42 ◽  
Author(s):  
M. Beecher ◽  
R. Baumont ◽  
J Aufrère ◽  
T.M. Boland ◽  
M.O’ Donovan ◽  
...  
Keyword(s):  
Organic Matter ◽  
Perennial Ryegrass ◽  
In Vitro Methods ◽  
Organic Matter Digestibility

scholarly journals In Vivo and in Vitro Digestibility of Four Tropical Grass Species Growing at Ibadan

2021 ◽  
Vol 2 (1) ◽  
pp. 89-94
Author(s):  
F. O. Olubajo ◽  
V. O. Tenabe
Keyword(s):  
Organic Matter ◽  
Pennisetum Purpureum ◽  
Grass Species ◽  
In Vitro Digestibility ◽  
Organic Matter Digestibility ◽  
Cynodon Nlemfuensis ◽  
Positive Correlations ◽  
Tropical Grass

Digestibility trials carried out in November and December, 1971 with twelve West African dwarf ram fed Cynodon nlemfuensis var. robustus; Cynodon nlemfuensis var. nlemfuensis: Pennisetum purpureum Schum; and Panieum maximum, Jacq, each at 4 weeks and 8 weeks of regrowth showed that the differences that may exist between methods and among treatments in organic matter digestibility were not statistically significant (P> 0.05). Similarly, analysis showed no significant differences among animals or between periods within each treatment except for Cynodon  nlemfuensis  var. nlemfuensis where differences between period were significant (P <0.01). There were high positive correlations between in vitro and in vivo organic matter digestibilities as well as between in vitro organic matter digestibility and Total Digestible Nutrients (TDN).  It is suggested that in vitro organic matter digestibility can be used to predict the digestibility of these tropical grass species and, may be, other similar grass species.

The prediction of digestibility by rumen fluid and enzymatic methods

1996 ◽  
Vol 1996 ◽  
pp. 211-211
Author(s):  
Peter Young ◽  
F. P. O'Mara ◽  
M. Rath ◽  
P. J. Caffrey
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Organic Matter Digestibility ◽  
Enzymatic Methods

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

The prediction of digestibility by rumen fluid and enzymatic methods

1996 ◽  
Vol 1996 ◽  
pp. 211-211
Author(s):  
Peter Young ◽  
F. P. O'Mara ◽  
M. Rath ◽  
P. J. Caffrey
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Organic Matter Digestibility ◽  
Enzymatic Methods

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

scholarly journals In vitro techniques for the assessment of the nutritive value of feed grains for pigs: a review

1999 ◽  
Vol 50 (5) ◽  
pp. 871 ◽  
Author(s):  
Paul J. Moughan
Keyword(s):  
Nutritive Value ◽  
Protein Digestibility ◽  
In Vitro Assays ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Ileal Digestibility ◽  
Plant Fibre ◽  
Gross Energy

The philosophy inherent in developing in vitro digestibility assays for dietary energy and protein is reviewed and an historical account is given of the development of such assays for the pig. General principles to be considered in the development of in vitro digestibility assays are discussed, as are limitations of the in vitro approach. The importance of choosing the most appropriate in vivo measures of digestibility for the evaluation of in vitro assays is stressed. For protein sources that do not contain anti-nutritional factors or plant fibre, ‘true’ ileal digestibility should be the in vivo baseline, while plant proteins should be tested against ‘real’ ileal digestibility. There is a dearth of adequately conducted validation studies for in vitro digestibility assays. It appears that the 3-step (pepsin, pancreatin, Viscozyme) closed in vitro system to allow prediction of organic matter and gross energy digestibility in the pig has particular promise for practical feed evaluation. Similarly based protein digestibility assays may require further development before they can be applied with confidence.

Repeatability of in vitro digestibility assays of forages when using fresh, frozen or freeze-dried cow faeces instead of sheep rumen liquor as sources of micro-organisms

1995 ◽  
Vol 1995 ◽  
pp. 110-110 ◽  
Author(s):  
S Akhter ◽  
E Owen ◽  
M K Theodorou ◽  
S L Tembo ◽  
E R Deaville
Keyword(s):  
Freeze Drying ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
Two Stage ◽  
Freeze Dried ◽  
Fresh Frozen ◽  
Sheep Rumen ◽  
Micro Organisms

Previous studies (El Shaer, Omed and Axford, 1987; Akhter, Owen, Fall, O'Donovan and Theodorou, 1994) with the two-stage in vitro procedure of Tilley and Terry (1963) have shown a high correlation between digestibilities of forages as determined using either sheep rumen liquor, sheep faeces or cow faeces as the microbial inoculum. In the first study of the of the present investigation one objective was to examine the repeatability of these digestibility measurements when made on different occasions. A second objective was to assess whether the correlations between faecal and rumen fluid based inocula could be improved if microorganisms were obtained from pairs rather than individual animals. The objective in the second study using forages of known in vivo digestibility, was to investigate the effect of freezing or freeze-drying of faeces on the repeatability of digestibilities of forages determined in vitro using micro-organisms from cow faeces.

scholarly journals Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum

2016 ◽  
Vol 56 (11) ◽  
pp. 1867 ◽  
Author(s):  
Erika C. Lara ◽  
Fernanda C. Basso ◽  
Flávia B. de Assis ◽  
Fernando A. Souza ◽  
Telma T. Berchielli ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacillus Subtilis ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Corn Silage ◽  
In Vitro Digestibility ◽  
Aerobic Stability ◽  
Organic Matter Digestibility ◽  
Corn Plants

Chemical composition, fermentation characteristics, in vitro digestibility and aerobic stability were evaluated in corn silage inoculated with microbial additives in two different experiments. Inoculant treatments (untreated, Bacillus subtilis and B. subtilis combined with Lactobacillus plantarum) were applied to fresh forages. Chopped corn plants (2B655 Hx) were ensiled in laboratory silos for periods of 7, 14, 21 and 63 days to evaluate the fermentation parameters. The experimental silos were weighed to determine gas losses. After the ensiling period, the silage was sampled to determine chemical composition and in vitro organic matter digestibility. To evaluate aerobic stability, chopped corn plants (AG‐1051) were ensiled in laboratory silos that were opened after 96 days of ensiling. The silage was placed in different buckets containing data loggers. The silage was sampled after 0, 4, 8 and 12 days of exposure to air to evaluate the microbial populations and pH. The data were analysed as a completely randomised design using a mixed repeated-measures model in the MIXED procedure of SAS. To evaluate each treatment relative to the fermentation times, a regression analysis using the PROC REG procedure of SAS was applied. A significance level of P < 0.05 was used. Inoculation with both strains increased lactic acid concentration, whereas the use of B. subtilis alone or combined with L. plantarum improved in vitro apparent organic matter digestibility. In the B. subtilis and B. subtilis combined with L. plantarum silages, moulds and yeasts decreased, and aerobic stability was improved. Inoculation with B. subtilis alone or combined with L. plantarum improved the nutritional value and aerobic stability of corn silage.

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