scholarly journals Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig 

2012 ◽  
Vol 50 (No. 3) ◽  
pp. 119-130 ◽  
Author(s):  
M. Klimczuk ◽  
J. Kaleczyc ◽  
A. Franke-Radowiecka ◽  
K. Czaja ◽  
P. Podlasz ◽  
...  
Keyword(s):  
Vasoactive Intestinal Polypeptide ◽  
Sensory Nerve ◽  
Biologically Active ◽  
Cholinergic Innervation ◽  
Vesicular Acetylcholine Transporter ◽  
Nerve Terminals ◽  
Nerve Fibres ◽  
Cholinergic Nerve ◽  
Active Substances ◽  
Immunohistochemical Characterisation

Our previous immunohistochemical investigations revealed three major populations of nerve fibres supplying the porcine accessory genital glands (AGG) including noradrenergic, non-noradrenergic putative cholinergic and sensory nerve terminals (Kaleczyc et al., 1997). However, it is still unclear whether the non-noradrenergic nerve fibres are cholinergic in nature. The knowledge of the population of cholinergic nerve fibres in mammalian AGG based upon vesicular acetylcholine transporter (VAChT) immunohistochemistry is very limited. Therefore, the aim of the present investigation was to disclose the occurrence and colocalization patterns of VAChT, dopamineâ-hydroxylase (DβH) and some neuropeptides including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and somatostatin (SOM) within nerve fibres supplying the porcine AGG. Double-immunohistochemical labellings showed that VAChT-positive nerve terminals were non-adrenergic (DâH-negative), however, many of them contained immunoreactivities to VIP, NPY and/or SOM. The coexistence patterns of these biologically active substances in nerve fibres supplying particular glands are similar but the density of cholinergic innervation varies between the organs. The innervation of the seminal vesicle and prostatic body is better developed than that of the disseminated part of the prostate and bulbourethral glands. The majority of cholinergic nerve fibres associated with blood vessels supplying the glands contain VIP and NPY and, to a lesser degree, SOM. The possible function and origin of the cholinergic nerve fibre population are discussed.

scholarly journals Neurochemical characteristics of paracervical ganglion in the pig

2008 ◽  
Vol 53 (No. 3) ◽  
pp. 135-146 ◽  
Author(s):  
P. Podlasz ◽  
K. Wasowicz
Keyword(s):  
Biologically Active ◽  
Small Cells ◽  
Vesicular Acetylcholine Transporter ◽  
Rt Pcr ◽  
Nerve Fibres ◽  
Sif Cells ◽  
Pituitary Adenylate Cyclase ◽  
Immunohistochemical Methods ◽  
Oxide Synthase ◽  
Active Substances

A study on the presence of the selected biologically active substances in nerve structures of the paracervical ganglion in the pig was performed with the use of immunofluorescence and RT-PCR. Immunohistochemical methods revealed that 23% of paracervical ganglion (PCG) neurons contain both tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DβH) and that the remaining 77% contain choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT). 73% of TH/DβH neurons contained neuropeptide Y (NPY) and 8% contained somatostatin (Som). All ChAT/VAChT positive neurons contained vasoactive intestinal polypeptide (VIP), 87% of them contained Som, 76% contained NPY and 32% contained neuronal nitric oxide synthase (nNOS). Galanin (Gal) was found only in small cells, which were thought to be SIF cells. No pituitary adenylate cyclase-activating polypeptide (PACAP)- or substance P (SP)-positive neurons were found in PCG. Some areas of PCG contained dense plexuses of ChAT- and VAChT-positive nerve fibres. In the ganglion small number of TH-, nNOS-, NPY-, VIP-, Gal-, PACAP-, Som- and SP-positive nerve fibres was also visible. RT-PCR detected the presence of mRNA for TH, ChAT, nNOS, NPY, VIP, Gal and Som, which were visualised as clearly discernible bands on a gel. In cases of PACAP and SP only weak bands were observed.

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

2006 ◽  
Vol 54 (1) ◽  
pp. 71-83 ◽  
Author(s):  
M. B. Arciszewski ◽  
K. Wąsowicz
Keyword(s):  
Tyrosine Hydroxylase ◽  
Distribution Patterns ◽  
Cholinergic Innervation ◽  
Vesicular Acetylcholine Transporter ◽  
Nerve Fibres ◽  
Similar Frequency ◽  
Muscular Layer ◽  
Noradrenergic Innervation ◽  
Sexual Glands ◽  
Male Sheep

Immunohistochemistry was applied to determine the distribution patterns of nerve fibres containing tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and vesicular acetylcholine transporter (VAChT) in the prostate, seminal vesicle (SV) and bulbourethral glands (BU) of male sheep. In all organs studied, cholinergic innervation was more developed than noradrenergic innervation. Numerous VAChT-immunoreactive (IR) nerve fibres were found in the muscular layer and mucosa of the SV and BU as well as in the prostate. A similar abundance of noradrenergic nerve fibres (showing immunoreactivity both to TH and DβH) was also found in both layers of the SV and BU (but not in the prostate). In the prostate a moderate density of VIP-IR nerve fibres was present but only very scarce NPY-IR nerve fibres were shown. All the studied accessory sexual glands (ASG) of male sheep contained VIP-IR nerve fibres in a similar frequency. Double immunohistochemistry revealed that the vast majority of noradrenergic nerve fibres also contained NPY. None of the noradrenergic nerve fibres showed the presence of VAChT or VIP. The possible functional significance of these findings is discussed.

Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

2017 ◽  
Vol 20 (1) ◽  
pp. 173-183 ◽  
Author(s):  
A. Zacharko-Siembida ◽  
M. Matysek ◽  
R. Szalak ◽  
A. Radlińska ◽  
K. Obszańska ◽  
...  
Keyword(s):  
Distribution Patterns ◽  
Neuroendocrine Cells ◽  
Biologically Active ◽  
Nerve Terminals ◽  
Nerve Fibres ◽  
Sif Cells ◽  
Pituitary Adenylate Cyclase ◽  
Close Proximity ◽  
The Brain ◽  
Pelvic Ganglia

Abstract Cocaine- and amphetamine-regulated transcript (CART) peptides are widely expressed not only in the brain but also in numerous endocrine/neuroendocrine cells as well as in neurons of the peripheral nervous system. The present study investigated the distribution patterns of CART-like immunoreactivity in the pelvic plexus (PP) of the female pig. The co-expression of CART with principal neurotransmitter markers: choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), serotonin (5-HT) or biologically active neuropeptides: pituitary adenylate cyclase-activating polypeptide (PACAP), substance P (SP), calbindin was analyzed using double immunohistochemical stainings. Amongst neurons immunopositive to Hu C/D panneuronal marker as many as 4.1 ± 1.2% in right and 4.4 ± 1.6% in left pelvic ganglia were found to express CART. The vast majority of CART-IR ganglionic neurons were predominantly small in size and were evenly scattered throughout particular ganglia. Immunoreactivity to CART was also detected in numerous nerve terminals (which frequently formed pericellular formations around CART-negative perikarya) as well as in numerous nerve fibres within nerve branches interconnecting the unilateral pelvic ganglia. Immunohistochemistry revealed that virtually all CART-IR neurons were cholinergic in nature and CART-IR basket-like formations frequently encircled TH-positive/CART-negative perikarya. None of CART-IR ganglionic neurons showed immunoreactivity to SP, PACAP, 5-HT or calbindin. CART-IR nerve fibres ran in a close vicinity to serotonin-containing cells or faintly labelled SP-expressing neurons. On the other hand, PACAP-IR, SP-IR (but not 5-HT-positive) nerve terminals were found to run in close proximity to CART-IR neurons. Our results indicate that: 1) CART present in PP may influence the activity of pelvic ganglionic neurons/SIF cells, 2) PP should be considered as a potential source of CART-like supply to pelvic viscera and 3) functional interactions between CART and SP or PACAP are possible at the periphery.

Innervation of papilla vallata in diabetic rats

2013 ◽  
Vol 154 (12) ◽  
pp. 443-448 ◽  
Author(s):  
Helén Hevér ◽  
Károly Altdorfer ◽  
Tivadar Zelles ◽  
Bayarchimeg Batbayar ◽  
Erzsébet Fehér
Keyword(s):  
Mast Cells ◽  
Substance P ◽  
Neuropeptide Y ◽  
Vasoactive Intestinal Polypeptide ◽  
Insulin Treatment ◽  
Diabetic Rats ◽  
Taste Buds ◽  
Nerve Terminals ◽  
Nerve Fibres ◽  
Intestinal Polypeptide

Introduction: Abnormal sensations such as pain and impairment of taste are symptoms of approximately 10% of patients having diabetes mellitus. Aim: The aim of the study was to investigate and quantify the different neuropeptide containing nerve fibres in the vallate papilla of the diabetic rat. Methods: Immunohistochemical methods were used to study the changes of the number of different neuropeptide containing nerve terminals located in the vallate papillae in diabetic rats. Diabetes was induced in the rats with streptozotocin. Results: Two weeks after streptozotocin treatment the number of the substance P, galanin, vasoactive intestinal polypeptide and neuropeptide Y immunoreactive nerve terminals was significantly increased (p<0.05) in the tunica mucosa of the tongue. The number of the lymphocytes and mast cells was also increased significantly. Some of the immunoreactive nerve terminals were located in the lingual epithelium both intragemmally and extragemmally and were seen to comprise dense bundles in the lamina propria just beneath the epithelium. No taste cells were immunoreactive for any of the investigated peptides. Vasoactive intestinal polypeptide and neuropeptide Y immunoreactive nerve fibres were not detected in the taste buds. For weeks after streptozotocin administration the number of the substance P, calcitonin gene related peptide and galanin immunoreactive nerve terminals was decreased both intragemmally and intergemmally. In case of immediate insulin treatment, the number of the immunoreactive nerve terminals was similar to that of the controls, however, insulin treatment given 1 week later to diabetic rats produced a decreased number of nerve fibers. Morphometry revealed no significant difference in papilla size between the control and diabetic groups, but there were fewer taste buds (per papilla). Conclusions: Increased number of immunoreactive nerve terminals and mast cells 2 weeks after the development of diabetes was the consequence of neurogenic inflammation which might cause vasoconstriction and lesions of the oral mucosa. Taste impairment, which developed 4 weeks after streptozotocin treatment could be caused by neuropathic defects and degeneration or morphological changes in the taste buds and nerve fibres. Orv. Hetil., 2013, 154, 443–448.

scholarly journals Localization of immunoreactivities for neuropeptides and neurotransmitter-synthesizing enzymes in the pterygopalatine ganglion of the pig

2012 ◽  
Vol 48 (No. 4) ◽  
pp. 99-107
Author(s):  
P. Podlasz ◽  
K. Wąsowicz ◽  
J. Kaleczyc ◽  
M. Łakomy ◽  
R. Bukowski
Keyword(s):  
Nerve Fibers ◽  
Biologically Active ◽  
Rt Pcr ◽  
Nerve Fibres ◽  
Single Nerve Cell ◽  
Weak Staining ◽  
Single Nerve ◽  
Pterygopalatine Ganglion ◽  
Strong Immunoreactivity ◽  
Active Substances

Study on the presence of the selected biologically active substances in nerve structures of the porcine pterygopalatine ganglion was performed with the use of immunofluorescence and RT-PCR. All neurons in the ganglion were ChAT-, VAChT-, NOS- and VIP- positive. However, some neurons displayed strong immunoreactivity, while in other neurons, immunoreactivity was moderate, or weak. Somatostatin (SOM) was present in approx. 11% of neurons. Tyrosine hydroxylase-positive (TH-positive) neurons were not detected, although in single nerve cell bodies, TH antibody revealed very weak staining which could be attributed to some residual TH immunoreactivity. Immunoreactivity to NPY was found in 25% of all neuronal perikarya while PACAP was present only in 2&ndash;3% of them. More numerous neurons (6%) contained immunoreactivity to GAL. No neurons stained for SP or CGRP. Numerous ChAT-, VAChT-, NOS-, VIP-, and PACAP-positive, scarce SP and CGRP-positive, single SOM-, NPY- and GAL-positive nerve fibers were observed throughout the ganglion. No TH immunoreactivity was found in the nerve fibres. RT-PCR detected strong signal of the transcripts of ChAT, SOM, NOS, VIP, NPY, PACAP, and GAL. Only very weak signal was observed in case of TH, SP and CGRP. No RT-PCR was performed for VAChT message.

Neutral Lipids of Oats Fruit (Avena Sativa L.)

2020 ◽  
Vol 9 (4) ◽  
pp. 40-43
Author(s):  
N. K. Yuldasheva ◽  
S. D. Gusakova ◽  
D. Kh. Nurullaeva ◽  
N. T. Farmanova ◽  
R. P. Zakirova ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Neutral Lipids ◽  
The Body ◽  
Biologically Active ◽  
Absorption Bands ◽  
Vital Functions ◽  
The Republic ◽  
Living Organisms ◽  
Main Components ◽  
Active Substances

Introduction. Lipids are a widespread group of biologically active substances in nature, making up the bulk of the organic substances of all living organisms. They accumulate in plants in seeds, as well as in fruits and perform a number of vital functions: they are the main components of cell membranes and the energy reserve for the body.Aim. Study of neutral lipids of sown oats (Avena sativa L.).Materials and methods. The objects of the study were fruits (grains) of oats of the sown variety "Tashkent 1," harvested in the Republic of Uzbekistan. Results and discussions. Neutral lipids of oat grains have been found to contain 13 fatty acids with a predominance of the sum of oleic, linolenic and linoleic acids. The total degree of unsaturation was almost 78%. Absorption bands characteristic of these substances were observed in the IR spectrum of MEGC.Conclusion. According to the results of the NL analysis, oat grains consisted of triacylglycerides and free LCDs, which were accompanied by hydrocarbons, phytosterols, triterpenoids and tocopherols.

Microscopic fungi of lithobiont communities of Argentine Islands Region: Data from the 22nd Ukrainian Antarctic Expedition

2020 ◽  
Vol 62 (1-2) ◽  
pp. 49-68
Author(s):  
T. O. Kondratiuk ◽  
T. V. Beregova ◽  
I. Yu. Parnikoza ◽  
S. Y. Kondratyuk ◽  
A. Thell
Keyword(s):  
Filamentous Fungi ◽  
Mucor Circinelloides ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Microscopic Fungi ◽  
Rhodotorula Rubra ◽  
Yeast Fungi ◽  
Antarctic Expedition ◽  
Geomyces Pannorum ◽  
Active Substances

The identification of the diversity of microscopic fungi of lithobiont communities of the Argentine Islands in specimens collected during the 22nd Ukrainian Antarctic Expedition was the purpose of this work. Samples of rock, soil, mosses and lichens of rock micro-habitats of “Crustose lichen sub-formation and fruticose lichen and moss cushion sub-formation” were used in the work. These samples were used for extracting and cultivation of filamentous fungi on dense nutrient media. Determination of physiological and biochemical characteristics and identification of yeast-like fungi were performed using a microbiological analyser ‘Vitek-2’ (‘Bio Merieux’, France). Cultivation of microorganisms was carried out at temperatures from +2 to +37 °C. In results cultures of microscopic fungi of Zygomycota (Mucor circinelloides), Ascomycota (species of the genera cf. Tlielebolus, Talaromyces), representatives of the Anamorphic fungi group (Geomyces pannorum, species of the genera Alternaria, Acremonium, Aspergillus, Penicillium, and Cladosporium) were isolated from Antarctic samples. Microscopic fungi Penicillium spp. were dominated after the frequency in the studied samples (54.5%). Rhodotorula rubra and Candida sp. among isolated yeast fungi, and dark pigmented fungi represented by Aureobasidium pulhdans and Exophiala spp. were identified. The biological properties of a number of isolated fungi (the potential ability to synthesise important biologically active substances: melanins, carotenoids, lipids) are characterised. Mycobiota of rock communities of Argentine Islands is rich on filamentous and yeast fungi similarly to other regions of Antarctica. A number of fungi investigated are potentially able to synthesise biologically active substances. The dark pigmented species of the genera Cladosporium, Exophiala, Aureobasidium pulhdans, capable of melanin synthesis; ‘red’ yeast Rhodotorula rubra (carotenoid producers and resistant to toxic metals); Mucor circinelloides and Geomyces pannorum, lipid producers, are among these fungi. Yeast-like fungi assimilated a wide range of carbohydrates, which will allow them to be further used for cultivation in laboratory and process conditions. The collection of technologically promising strains of microorganisms, part of the Culture Collection of Fungi at Taras Shevchenko National University of Kyiv (Ukraine), is updated with isolated species (strains) of filamentous fungi and yeast – potential producers of biologically active substances, obtained within this study.

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