scholarly journals Tuberculous lesions in pig lymph nodes caused by kaolin fed as a supplement

2012 ◽  
Vol 49 (No. 10) ◽  
pp. 379-388 ◽  
Author(s):  
L. Matlova ◽  
L. Dvorska ◽  
M. Bartos ◽  
J. Docekal ◽  
M. Trckova ◽  
...  
Keyword(s):  
Surface Water ◽  
Lymph Nodes ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Three Samples ◽  
Potential Sources ◽  
Sources Of Infection ◽  
On Farm ◽  
Slaughtered Pigs

An increased incidence of tuberculous lesions in head and mesenteric lymph nodes from slaughtered pigs weighing about 115 kg was recorded in a herd of pigs kept in two farms A and B in the CzechRepublic. Tuberculous lesions were more frequently (P < 0.01) diagnosed in pigs from Farm A (10.4%) than from Farm B (1.1%). The follow-up investigation of potential sources of infection on Farm A revealed that the piglets were fed kaolin from a nearby mine as a supplement. Among 20 samples from the pigs’ environment, atypical conditionally pathogenic mycobacteria (ACPM) were detected in four samples as follows: dust (n = 2), pig faeces (n = 1) and kaolin fed as a supplement (n = 1). Mycobacterium avium subsp. hominissuis of genotype IS901– and IS1245+ and serotype 8 was isolated from kaolin and pig lymph nodes, M. fortuitum from stable dust and pig faeces and other ACPM from stable dust. When kaolin feeding to piglets ceased, the incidence of tuberculous lesions in these pigs at slaughter 5–6 months later decreased from 16.1% to 3.4%. No ACPM was detected on Farm B in 27 samples from the environment. An investigation of surface kaolin mines did not detect mycobacteria in non-extracted kaolin. However, surface water (three isolates among 13 samples) from the pond used for kaolin levigation and 23 batches of the final product of kaolin (10 samples from each batch, i.e. a total of 230 samples were examined) were contaminated with ACPM. Among the latter, ACPM were isolated from three samples originating from three different batches. ACPM were likely to survive during transport of the kaolin, as a suspension through the pipeline, and during its further processing to the final product (sedimentation, addition of colloid substances, drying and other procedures).

Mycobacterium avium subsp. avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

2014 ◽  
Vol 77 (1) ◽  
pp. 141-144 ◽  
Author(s):  
PETR KRIZ ◽  
MARIJA KAEVSKA ◽  
IVA SLANA ◽  
IVA BARTEJSOVA ◽  
IVO PAVLIK
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Mesenteric Lymph ◽  
Qpcr Method ◽  
Pig Carcasses ◽  
Slaughtered Pigs

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)–like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (102 to 103 cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.

scholarly journals Bacteriologicae Studies on Tuberculous Lesions in the Mesenteric Lymph Nodes of slaughtered pigs

1972 ◽  
Vol 25 (2) ◽  
pp. 76-84 ◽  
Author(s):  
Tsukasa MIYASHITA ◽  
S YACHIDA ◽  
H NARA ◽  
K SHIMIZU ◽  
T ONO
Keyword(s):  
Lymph Nodes ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Slaughtered Pigs

scholarly journals Mesenteric lymphadenopathy in children with recurrent abdominal pain

2017 ◽  
Vol 4 (4) ◽  
pp. 1525
Author(s):  
Saransh Sabal ◽  
Lakhan Poswal ◽  
Sandip Gediya ◽  
Suresh Goyal
Keyword(s):  
Abdominal Pain ◽  
Lymph Nodes ◽  
Recurrent Abdominal Pain ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymphadenitis ◽  
Medical College ◽  
Mesenteric Lymph ◽  
Pain Abdomen ◽  
Group 5

Background: Enlarged mesenteric lymph nodes (MLN) are frequently seen in children with abdominal pain and in the absence of other disorders, have been attributed to primary mesenteric lymphadenitis. Mesenteric lymphadenitis is commonly reported in children with acute, chronic or recurrent abdominal pain and no evidence of other pathologies. The purpose of this study was to find out the association of USG finding of enlarged mesenteric lymph nodes with causation of recurrent abdominal pain and outcome of these patients on follow up.Methods: This hospital based prospective study was done on 82 patients aged 5 to 15 years, attending Bal Chikitsalaya, RNT Medical college, Udaipur, fulfilling Apley’s criteria of RAP. Detailed history, physical examination, relevant investigations and USG abdomen was done in all patients.Results: Eighty-two children were enrolled; out of which majority (62.19%) were in age group 5-8 years with mean age 7.9±2.69 years. Mesenteric lymphadenopathy was found in 14 (17.07%) cases. All patients with mesenteric lymphadenopathy 14 (100%) had periumbilical pain and diurnal variation of pain. Follow up of 14 patients who had mesenteric lymphadenopathy showed that in 12 (85.71%) patients, the mesenteric lymphadenopathy resolved on USG despite that pain abdomen persisted in 7 (58.33%).Conclusions: The presence of mesenteric lymphadenopathy can’t be attributed to recurrent abdominal pain.

scholarly journals Prevalence of Salmonellae and their resistance to antibiotics in slaughtered pigs in the Czech Republic

2011 ◽  
Vol 22 (No. 6) ◽  
pp. 230-236 ◽  
Author(s):  
F. Šišák ◽  
H. Havlíčková ◽  
R. Karpíšková ◽  
I. Rychlík
Keyword(s):  
Czech Republic ◽  
Lymph Nodes ◽  
Resistance Genes ◽  
The Other ◽  
Mesenteric Lymph Nodes ◽  
The Czech Republic ◽  
Resistance To Antibiotics ◽  
Mesenteric Lymph ◽  
Frequent Site ◽  
Slaughtered Pigs

Salmonella prevalence was assessed in 816 pigs from fifteen herds which were slaughtered in ten slaughterhouses from June 2001 to December 2002. No Salmonellae were isolated in pigs from eight herds in four slaughterhouses. Salmonella prevalence in pigs originating from the other seven herds ranged from 2.0% to 12.0%. The most frequent site of Salmonella isolation was caecum (2.45%). This finding is statistically significant (P &lt; 0.01) as compared to those obtained with mesenteric lymph nodes (0.73%) and carcass swabs (0.12%). Salmonellae were not found in samples from the environments (n = 197). A total of 27 Salmonella isolates were classified into serotypes S. infantis (n = 8), S. typhimurium (n = 5), S. agona (n = 4), S. kaapstad (n = 4), S. derby (n = 3), S. bredeney (n = 2), and S. london (n = 1). All five S. typhimurium DT 104 were resistant to the phenotype ACSSuT. Resistance genes bla<sub>PSE-1</sub>, floR, aadA2, sul1, and tetG were identified in all pentaresistant strains. One strain of S. derby was resistant to gentamicin, streptomycin and sulphonamides. The other Salmonella isolates were sensitive to all antibiotics tested. &nbsp;

In situ demonstration of migration of dendritic cells released from rat small intestine to mesenteric lymph nodes

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Intestine ◽  
Lymph Nodes ◽  
Rat Small Intestine ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

scholarly journals The microbiota is dispensable for the early stages of peripheral regulatory T cell induction within mesenteric lymph nodes

2021 ◽  
Author(s):  
Carolin Wiechers ◽  
Mangge Zou ◽  
Eric Galvez ◽  
Michael Beckstette ◽  
Maria Ebel ◽  
...  
Keyword(s):  
T Cell ◽  
Lymph Nodes ◽  
Regulatory T Cell ◽  
De Novo ◽  
Bacterial Species ◽  
Short Chain Fatty Acids ◽  
Mesenteric Lymph Nodes ◽  
Direct Role ◽  
Early Stages ◽  
Mesenteric Lymph

AbstractIntestinal Foxp3+ regulatory T cell (Treg) subsets are crucial players in tolerance to microbiota-derived and food-borne antigens, and compelling evidence suggests that the intestinal microbiota modulates their generation, functional specialization, and maintenance. Selected bacterial species and microbiota-derived metabolites, such as short-chain fatty acids (SCFAs), have been reported to promote Treg homeostasis in the intestinal lamina propria. Furthermore, gut-draining mesenteric lymph nodes (mLNs) are particularly efficient sites for the generation of peripherally induced Tregs (pTregs). Despite this knowledge, the direct role of the microbiota and their metabolites in the early stages of pTreg induction within mLNs is not fully elucidated. Here, using an adoptive transfer-based pTreg induction system, we demonstrate that neither transfer of a dysbiotic microbiota nor dietary SCFA supplementation modulated the pTreg induction capacity of mLNs. Even mice housed under germ-free (GF) conditions displayed equivalent pTreg induction within mLNs. Further molecular characterization of these de novo induced pTregs from mLNs by dissection of their transcriptomes and accessible chromatin regions revealed that the microbiota indeed has a limited impact and does not contribute to the initialization of the Treg-specific epigenetic landscape. Overall, our data suggest that the microbiota is dispensable for the early stages of pTreg induction within mLNs.

Sign in / Sign up

Export Citation Format

Share Document