scholarly journals  Infection in a female Komodo dragon (Varanus komodoensis) caused by Mycobacterium intracellulare: a case report

2012 ◽  
Vol 57 (No. 3) ◽  
pp. 163-168 ◽  
Author(s):  
M. Skoric ◽  
V. Mrlik ◽  
J. Svobodova ◽  
V. Beran ◽  
M. Slany ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Plasma Cells ◽  
Histopathological Examination ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Mycobacterium Intracellulare ◽  
Granulomatous Pneumonia ◽  
Variable Layer

In early 2002, a bean-like whitish nodule 2 × 1 × 1 cm in size was diagnosed on the tongue of a female Komodo dragon (Varanus komodoensis) kept in a zoological garden in the Czech Republic. The nodule was removed at surgery and histopathological examination revealed a specific granulomatous inflammation. The granuloma contained a necrotic mass surrounded by a variable layer of palisading epithelioid macrophages and multinucleated giant cells with a variable admixture of lymphocytes and plasma cells. Signs of mineralisation within the granuloma were not observed. Using Ziehl-Neelsen (ZN) staining the presence of acid-fast rods (AFR) was determined and the presence of mycobacteria was confirmed by PCR. Four years later, this female died after multiple injuries caused by a male during mating. Necropsy and histopathological examinations revealed granulomatous pneumonia and myocarditis with ZN-positive AFR within granulomas. Mycobacteria were cultured from 13 of 19 tissue samples: Mycobacterium intracellulare was confirmed by sequencing of isolates from multiple affected organs including the respiratory tract, tail muscle, inguinal lymph nodes and blood; Mycobacterium sp. were also isolated from the tongue. In addition, mycobacteria were detected in 15 (46.9%) of 32 environmental samples examined in both years. M. intracellulare was detected in water sediment from the female’s terrarium in 2002, and in faeces and peat from the terrarium and in water sediment from the male terrarium in 2006. Except for M. intracellulare (n = 4), M. smegmatis (n = 1), M. a. hominissuis (n = 3), M. fortuitum (n = 2), M. interjectum (n = 1), M. peregrinum/alvei/septicum (n = 1) and Mycobacterium sp. (n = 2) were also isolated from different environmental samples.    

scholarly journals  Avian tuberculosis in a captured Ruppell's griffon vulture (Gyps ruppellii): a case report

2010 ◽  
Vol 55 (No. 7) ◽  
pp. 348-352 ◽  
Author(s):  
M. Skoric ◽  
P. Fictum ◽  
L. Frgelecova ◽  
P. Kriz ◽  
I. Slana ◽  
...  
Keyword(s):  
Plasma Cells ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Body Cavity ◽  
The Body ◽  
Multinucleated Giant Cells ◽  
Quantitative Real Time Pcr ◽  
Griffon Vulture ◽  
Avium Infection ◽  
Variable Layer

Avian tuberculosis was diagnosed in a captured female Ruppell's griffon vulture (Gyps ruppellii) with granulomatous splenitis and hepatitis. At necropsy, whitish to yellow nodules of various sizes were found in the spleen and liver, and fibrinous coelomitis was present in the body cavity. Histopathologically, the granulomas appeared to be typical of avian tuberculosis. In some granulomas, necrotic centres surrounded by a variable layer of palisading epithelioid macrophages and multinucleated giant cells with variable admixture of lymphocytes and plasma cells were present. Signs of mineralization in granulomas were not observed. Using Ziehl-Neelsen staining the presence of acid-fast bacilli was demonstrated in organs affected by granulomatous inflammation. A diagnosis of Mycobacterium avium subsp. avium infection was confirmed by culture and quantitative Real-Time PCR examination for the presence of specific insertion sequences for avian tuberculosis (IS901 and IS1245) in the liver and spleen.  

scholarly journals Xanthogranulomatous Endometritis: A Challenging Imitator of Endometrial Carcinoma

2007 ◽  
Vol 2007 ◽  
pp. 1-3 ◽  
Author(s):  
A. Işın Doğan-Ekici ◽  
Alp Usubütün ◽  
Türkan Küçükali ◽  
Ali Ayhan
Keyword(s):  
Endometrial Carcinoma ◽  
Genital Tract ◽  
Plasma Cells ◽  
Histopathological Examination ◽  
Female Genital Tract ◽  
Giant Cells ◽  
Polymorphonuclear Leucocytes ◽  
Xanthogranulomatous Inflammation ◽  
Post Menopausal ◽  
Female Genital

Xanthogranulomatous inflammation is a distinguished histopathological entity affecting several organs, predominantly the kidney and gallbladder. So far, only a small number of cases of xanthogranulomatous inflammation occurring in female genital tract have been described, most frequently affecting the endometrium and histologically characterized by replacement of endometrium by xanthogranulomatous inflammation composed of abundant foamy histiocytes, siderophages, giant cells, fibrosis, calcification and accompanying polymorphonuclear leucocytes, plasma cells and lymphocytes of polyclonal origin. We present a case of a 69-year-old female complained of post menopausal bleeding and weight loss. Clinical preliminary diagnoses were endometrial carcinoma or hyperplasia and ultrasound was supposed to be endometrial malignancy, hyperplasia or pyometra by radiologist. Histopathological examination of uterus revealed xanthogranulomatous endometritis. Since xanthogranulomatous endometritis may mimic endometrial malignancy clinically and pathologically as a result of the replacement of the endometrium and occasionally invasion of the myometrium by friable yellowish tissue composed of histiocytes, knowledge of this unusual inflammatory disease is needed for both clinicians and pathologists.

scholarly journals A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2869
Author(s):  
Verónica Molina-Hernández ◽  
María T. Ruiz-Campillo ◽  
Francisco J. Martínez-Moreno ◽  
Leandro Buffoni ◽  
Álvaro Martínez-Moreno ◽  
...  
Keyword(s):  
Bile Ducts ◽  
Plasma Cells ◽  
Fasciola Hepatica ◽  
Vaccine Trial ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Control Group ◽  
Infected Control Group ◽  
Hepatic Lesions ◽  
Host Parasite

Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial.

scholarly journals  A case of systemic mycosis in a Hovawart dog due to Candida albicans

2011 ◽  
Vol 56 (No. 5) ◽  
pp. 260-264 ◽  
Author(s):  
M. Skoric ◽  
P. Fictum ◽  
I. Slana ◽  
P. Kriz ◽  
I. Pavlik
Keyword(s):  
Candida Albicans ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Abdominal Cavity ◽  
Giant Cells ◽  
Health Condition ◽  
Periodic Acid Schiff ◽  
Tissue Samples ◽  
Generalized Lymphadenopathy ◽  
Systemic Infections

Candida albicans is reported as the etiological agent of multi-systemic infections in dogs. A two-year-old female Hovawart dog was presented with marked alteration in its health condition characterised by weakness, fever, anorexia, abdominal pain, cachexy and generalized lymphadenopathy. A radiograph of the abdominal cavity showed several non-specific nodular lesions in the mesentery, ranging in size up to 10 cm in diameter. At necropsy, extensive enlargement of lymph nodes and the presence of numerous whitish to grey nodules of different sizes in several organs were evident. Histopathological examination revealed pyogranulomatous inflammation characterized by large areas of necrosis surrounded by neutrophilic granulocytes, macrophages, multinucleated giant cells, and a variable admixture of lymphocytes and fungi-like organismsin in all affected organs. Numerous branching hyphae, subsequently identified by mycological cultivation as Candida albicans, were observed. A periodic acid Schiff (PAS) reaction to prove the presence of fungi in tissues was positive. Examination of tissue samples of affected organs using polymerase chain reaction (quantitative Real-Time PCR) and cultivation was negative for the presence of all members of the Mycobacterium tuberculosis complex, M. avium subsp. avium and M. avium subsp. hominissuis.

A 62-Year-Old African American Woman With Stable Lymphadenopathy Over 5 Years

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S110-S110
Author(s):  
Hanadi El Achi ◽  
William Glass ◽  
Miriam Velazquez ◽  
Zhihong Hu
Keyword(s):  
African American ◽  
Plasma Cells ◽  
African American Woman ◽  
American Woman ◽  
Granulomatous Inflammation ◽  
Chronic Fatigue ◽  
Giant Cells ◽  
Castleman Disease ◽  
African American Female ◽  
Mantle Zone

Abstract The patient is a 62-year-old African American female with medical history of Plummer Vinson syndrome and CT scan in 2014 showing lymphadenopathy of the porta hepatis. Needle core biopsy of enlarged lymph nodes revealed sheets of polytypic plasma cells with no aberrant B or T cells or granulomas or hyaline-like depositions identified. The findings were suspicious for unicentric Castleman disease. In 2019, the patient presented with chronic fatigue without other systemic symptoms. Contrast CT revealed persistent retroperitoneal lymphadenopathy and calcified granulomas in the liver, spleen, and lung. Excision of portal and celiac nodes showed preserved nodal architecture with mild mantle zone expansion and marked hyaline-like deposition in the follicles. Sheets of mature plasma cells and focally increased vasculatures were seen in the interfollicular areas. Focally frequent nonnecrotizing granulomas were noted. Occasional giant cells and rare asteroid bodies were identified in the granulomas. Immunohistochemistry stain workup revealed normal distribution of the B and T lymphocytes. CD138 stain highlighted marked increased plasma cells and HHV8 was negative. Immunofluorescence microscopy for kappa and lambda did not show monoclonality; IgA was positive in <10% of plasma cells; special stain for Congo red and serum amyloid associated protein (SSA) IHC stain were strongly positive in the follicles. The overall findings are consistent with reactive lymphadenopathy with interfollicular polytypic plasmacytosis, noncaseating granuloma formation, and reactive amyloidosis. The features of mantle zone hyperplasia and polytypic plasmacytosis are suggestive of HHV8-negative/idiopathic unicentric Castleman disease. This case possibly represents an example of concurrent amyloidosis, sarcoidosis, and idiopathic unicentric Castleman disease. Given that SSA was recently reported to have a pathogenic role in granulomatous inflammation of sarcoidosis through sustained inflammatory reaction, the possibility of increased SSA triggering sarcoidoisis in this patient can be considered. However, the exact pathogenesis of these concurrent findings needs further investigation and clarification.

Equine Granulomatous Enteritis

1974 ◽  
Vol 11 (6) ◽  
pp. 535-547 ◽  
Author(s):  
R. E. Cimprich
Keyword(s):  
Weight Loss ◽  
Mycobacterium Tuberculosis ◽  
Plasma Cells ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Gut Content ◽  
Epithelioid Cells ◽  
History Of ◽  
Microscopic Change ◽  
Granulomatous Enteritis

The gross abnormalities in the intestines of 10 horses with a history of chronic weight loss varied greatly. The principal microscopic change was granulomatous inflammation characterized by lymphocytes, plasma cells, macrophages, epithelioid cells and giant cells. Mycobacterium tuberculosis, avian type, was isolated from the gut content of one horse, but no cause was found in the others. Horses previously reported to have tuberculosis had lesions similar to those described here. These lesions are also similar to those in Crohn's disease of man.

scholarly journals Clinicopathological study of granulomatous lobular mastitis

2019 ◽  
Vol 6 (3) ◽  
pp. 881
Author(s):  
Sapna Goel ◽  
Kanwar Singh Goel
Keyword(s):  
Histopathological Examination ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Contraceptive Pills ◽  
Mammary Duct ◽  
Uncommon Disease ◽  
Oral Steroids ◽  
Cystic Changes ◽  
Age Range

Background: Granulomatous lobular mastitis is an uncommon disease. This condition may resemble malignancy and tuberculosis. It is characterised by granulomatous inflammation with multinucleated giant cells, epithelioid histiocytes. The inflammation is centred on lobules. The purpose of this study is to review the clinical and pathological aspects in 8 cases of granulomatous lobular mastitis and review of pertinent literature.Methods: This is a prospective observational study. A total of 8 patients were studied. Investigations done. Patient were treated by erythromycin 250mg qid, tinidazole 500mg bid, for 7 days and steroids for two months. The lumps were excised, sinuses were excised and abscesses were drained, and oral steroids were given. Follow-up was done for 6 months.Results: Most of the patients were multiparous and lactating, with age range from 18 to 36 years. Clinically the lesion was felt as malignant in 3 patients and benign in 5 patients. Most patients were using contraceptive pills. There was suppuration in 3 patients and sinuses were found in 2 patients. Histopathological examination revealed granulomatous inflammation centred on lobules.Conclusions: The diagnosis of granulomatous lobular mastitis should be made very carefully to avoid any confusion with malignancy, tuberculosis, fungal infection, sarcoidosis, mammary duct ectasia, cystic changes in breast with over palpation and puerperal mastitis with over palpation. Though it is rare disease, but complete understanding by pathologist and surgeon is required for improving its identification.

scholarly journals Gastric lesions associated with the presence of Anisakis spp. Dujardin, 1845 (Nematoda: Anisakidae) in Cetaceans stranded on the coast of Ceara, Brazil

2008 ◽  
Vol 8 (2) ◽  
pp. 91-95 ◽  
Author(s):  
Monica Regina Alves Motta ◽  
Diana Célia Sousa Nunes Pinheiro ◽  
Vitor Luz Carvalho ◽  
Daniel de Araújo Viana ◽  
Ana Carolina Paulo Vicente ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Similar Distribution ◽  
Northeast Brazil ◽  
Gastric Lesions ◽  
Tissue Samples ◽  
Gastric Lumen ◽  
Anisakis Spp

The gastric compartments of ten cetaceans stranded on the coast of Ceará State, Northeast Brazil were analyzed in this study. Gastric Anisakis spp. was diagnosed in all individuals involved in this study. Parasites and tissue samples were collected during necropsy. The presence of Anisakis parasites showed similar distribution across the three gastric compartments and the majority was free within the gastric lumen. Macroscopically, the lesions were predominantly characterized by the presence of ulcers (60%, 6/10) within the gastric mucosa, occasionally associated with edema and hemorrhage (30%, 3/10). Eight cetaceans (8/10 - 80%) presented gastric microscopic alterations and in 75% (6/8) of these animals, chronic lymphoplasmocytic gastritis was observed with varying degrees of distribution and severity. Additionally, eosinophilic and granulomatous inflammation with giant cells, hemosiderosis, fibrosis and areas of necrosis were associated with location of parasites within the gastric mucosa. In this study, it was shown that the majority of cetaceans with the presence of Anisakis parasites presented macro and microscopic gastric alterations. These nematodes are probably associated with the development of these alterations; however, more pathological approaches are still required.

scholarly journals Variations in contamination by mercury, cadmium and lead on swine farms in the district of Hodonin in 1994 to 1999

2001 ◽  
Vol 46 (No. 5) ◽  
pp. 132-139 ◽  
Author(s):  
R. Ulrich ◽  
J. Raszyk ◽  
A. Nápravník
Keyword(s):  
Czech Republic ◽  
Environmental Samples ◽  
Animal Tissue ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Swine Farms ◽  
Liver And Kidney ◽  
The Mean ◽  
Cadmium And Lead ◽  
Feed Samples

Environmental samples (n&nbsp;= 254) were collected at regular intervals from 1994 to 1999 on three swine farms in the area of the district town Hodon&iacute;n, Czech Republic. The samples of feed mixtures (n&nbsp;= 85), barn dust sediments (n&nbsp;= 44), and muscular (n&nbsp;= 42), hepatic (n&nbsp;= 41), and renal (n&nbsp;= 42) tissues of feeder pigs were analysed for the concentrations of mercury, cadmium and lead. The concentrations of mercury showed decreasing tendencies in animal tissue samples (1994&ndash;1996). Compared with the mean for the Czech Republic, the concentration of mercury was markedly higher in liver and kidney samples (0.064 mg/kg and 0.114 mg/kg, respectively) collected in 1998, the concentration of cadmium was moderately higher in feed, muscle, liver, and kidney samples collected in 1996&ndash;1998. Statutory limits were exceeded in two liver and two kidney samples and one feed sample only. The concentration of lead in muscle, kidney, and feed samples (1994&ndash;1996) and barn dust samples (1996&ndash;1998) showed also a decreasing tendency. However, the limit was exceeded in 16 muscle samples (0.11 to 0.23&nbsp;mg/kg) collected in 1994&ndash;1999 and two kidney samples collected in 1996 (0.74 mg/kg) and 1999 (0.77 mg/kg). Heavy metals present in dust sediments apparently did not contribute significantly to their content in animal tissue. While the health risk resulting from the contents of mercury and cadmium can be classified as moderate, the contents of lead must be regarded as markedly more dangerous.

Superficial Granulomatous Pyoderma Gangrenosum Involving the Face: A Case Series of Five Patients and a Review of the Literature

2021 ◽  
pp. 120347542098886
Author(s):  
Eran Shavit ◽  
Michael Cecchini ◽  
James J. Limacher ◽  
Scott Walsh ◽  
Ashely Wentworth ◽  
...  
Keyword(s):  
Pyoderma Gangrenosum ◽  
Plasma Cells ◽  
Relevant Literature ◽  
Case Series ◽  
Granulomatous Inflammation ◽  
Final Diagnosis ◽  
Giant Cells ◽  
Neutrophilic Dermatosis ◽  
The Face ◽  
Inflammatory Infiltrates

Background Pyoderma gangrenosum (PG) is a rare neutrophilic dermatosis characterized by painful and ulcerating lesions on the skin. It rarely involves the face and is often difficult to diagnose. There are few cases reported in the literature of PG involving the face. Aim To share our experience with 5 patients in whom the final diagnosis was PG involving the face, and to review the literature. Methods We report a series of 5 patients with a final diagnosis of PG involving the face and reviewed relevant literature. We searched through PubMed and EMBASE using keywords such as “face” and “pyoderma gangrenosum,” “blastomycosis-like pyoderma gangrenosum, vegetative pyoderma gangrenosum and granulomatous pyoderma gangrenosum.” Results We report 5 patients (4 females) with pyoderma gangrenosum involving the face. All 5 had a final diagnosis of superficial granulomatous PG. All cases presented with nonhealing facial ulcer most commonly on cheeks and a common histopathology of mixed inflammatory infiltrates, multinucleated giant cells, and plasma cells with some granulomatous inflammation. Conclusions PG can involve the face, and all 5 of our patients had the superficial granulomatous as the most common form.

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