ASSESSMENT OF ANTIGENIC LOAD OF PIGS DURING PREGNANCY AND IDENTIFICATION OF SIGNS OF ISOIMMUNIZATION IN THE RECEIVED OFFSIDANCE

2020 ◽  
Vol 3 ◽  
pp. 95-99
Author(s):  
A.V. Agarkov ◽  
A.F. Dmitriev ◽  
A.N. Kvochko ◽  
N.V. Agarkov ◽  
A.R. Onishchenko
Keyword(s):  
Antigenic Load

scholarly journals FREQUENCY OF DETECTION OF LPS O-ANTIGENS OF DIFFERENT CAUSATIVE AGENTS IN PATIENTS WITH ACUTE DIARRHEAL DISEASES

2017 ◽  
Vol 22 (2) ◽  
pp. 75-79
Author(s):  
O. F Belaia ◽  
O. A Paevskaya ◽  
S. N Zuevskaya ◽  
E. G Korogodskaia ◽  
K. T Umbetova ◽  
...  
Keyword(s):  
High Frequency ◽  
Blood Donors ◽  
Clinical Course ◽  
Control Group ◽  
O Antigen ◽  
Intestinal Infections ◽  
Causative Agents ◽  
Elevated Rate ◽  
O Antigens ◽  
Antigenic Load

The aim is to determine the frequency and dynamics of detection of specific lipopolysaccharide (LPS) O-antigens LPS/O-antigens of causative agents in hospitalized diarrhea patients. Materials and methods. A total of 146 hospitalized diarrhea patients were examined with the use of an immunological method of the reaction of coagglutination (RCA) for the presence of LPS O-antigens of Shigella, Salmonella, Yersinia and Campylobacter in feces as markers of major intestinal infections pathogens. The control group was consisted of 40 blood donors. Results. In acute diarrhea patients there was detected the predominance of Yersinia and Salmonella LPS O-antigens over Shigella and Campylobacter; the high frequency of intestinal bacterial mixt-infection in total (68%), as well as elevated rate incidence of Shigella, Salmonella, Campylobacter, Yersinia antigens in mixt-infections in comparison with those in monoinfections (24%). The total O-antigen "load" in patients with mixt-infection was 3 times higher than in patients with monoinfections; with the increasing of LPS/O-antigen "load" the rate of Salmonella inoculation declines. Conclusion. Under the same severity of the clinical course of the disease, the decline of Salmonella inoculation rate in cases of high antigenic "load" may indicate to the presence of the phenomenon of summation of toxic effects of LPS O-antigens when the concentration of each pathogen in the feces is insufficient (for inoculation) for bacteriological examination.

LOCAL IMMUNE STATUS AND ETIOLOGICAL FACTORS IN PARATONSILLITIS

2019 ◽  
Vol 25 (3) ◽  
pp. 78-86
Author(s):  
T.V. Zolotova ◽  
◽  
A.G. Volkov ◽  
P.A. Kondrashov ◽  
◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Immunoglobulin A ◽  
Secretory Iga ◽  
Control Group ◽  
Local Immunity ◽  
Immunomodulatory Agents ◽  
Etiological Factors ◽  
Streptolysin O ◽  
Bacterial Lysates ◽  
Antigenic Load

Paratonsillitis is one of the most common purulent inflammatory diseases, considered as a manifestation of chronic tonsillitis. The aim of the work is to study the state of local immunity by the level of immunoglobulin A in saliva in patients with paratonsillitis and its changes in the treatment process, to determine the level of antistreptolysin-O in serum, as well as to study the etiological factors of paratonsillites in the microbiological assessment of the species composition of flora in smears from the cavity of paratonsillar abscess. Under our observation there were 152: 32 healthy people and 120 patients with paratonsillitis under abecedarian or abscess stage. It was found that in patients with paratonsillitis there is a significant (p<0.001) decrease in the level of secretory immunoglobulin A in saliva, which indicates a violation of local immunity and requires correction. The level of antibodies to streptolysin-O in the serum of patients with paratonsillitis was significantly - 10.25 times higher than in healthy individuals in the control group, which confirms the high streptococcal antigenic load. The results of microbiological examination of smears from the abscess cavity indicated the release of the most frequent pathogens of paratonsillites - Streptococcus β-haemolythicus, Streptococcus Pneumoniae and Staphylococcus Aureus, with bacterial associations prevailing (63.5%). After a course of antimicrobial therapy, supplemented with immunomodulatory agents in the form of bacterial lysates, there was an increase in the level of secretory IgA in saliva in 10-30 days in 3.3-4.5 times. At the same time, the level of antistreptolysin-O significantly decreased. In patients receiving immunomodulatory therapy in the form of bacterial lysates in the complex treatment of paratonsillitis, for the period of observation of 6 months, there was a decrease in the frequency of relapses of the disease by 2 times.

scholarly journals ANTI M. leprae IgM ANTIBODY DETERMINATION BY ULTRAMICROIMMUNOENZYMATIC (UMELISA HANSEN) FOR THE DIAGNOSIS AND MONITORING LEPROSY

1998 ◽  
Vol 40 (3) ◽  
pp. 177-182 ◽  
Author(s):  
Adriana TORRELLA ◽  
Rosa L. SOLIS ◽  
Esther PEREZ ◽  
Yadira MEDINA ◽  
Carlos KERGUELEN ◽  
...  
Keyword(s):  
Antibody Response ◽  
Clinical Improvement ◽  
Diagnostic Techniques ◽  
Igm Antibody ◽  
Smear Examination ◽  
Early Signal ◽  
Significant Clinical Improvement ◽  
Antibody Levels ◽  
The Relationship ◽  
Antigenic Load

The relationship between the IgM antibody response, antigenic load as well as the clinical improvement after chemotherapy was studied in order to obtain useful data for the early diagnosis and monitoring leprosy. A level of 82% (94/115) agreement was obtained between IgM UMELISA HANSEN and slitskin smear examination. Discrepant results were observed in 16 patients who showed positive IgM response despite negative by the skin smear examination. In these patients, the IgM response was seen to be associated to the early signal for bacilli recurrence in the skin. In one of these patients the presence of bacilli was demonstrated in the skin, two months after IgM antibodies being detected by UMELISA HANSEN. Also in one of the treated patients positive by both diagnostic techniques, a remarkable decrease in the IgM antibody levels was seen, correlating with a significant clinical improvement. Moreover it was found a direct relationship between the IgM antibody response and bacterial antigenic load, regardless the time elapsed in the disease's evolution.

scholarly journals Enhancing mucosal immunity by transient microbiota depletion

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Simone Becattini ◽  
Eric R. Littmann ◽  
Ruth Seok ◽  
Luigi Amoretti ◽  
Emily Fontana ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Infectious Agents ◽  
Colonization Resistance ◽  
Mucosal Vaccination ◽  
Mucosal Surfaces ◽  
Pathogen Entry ◽  
Portal Of Entry ◽  
Optimal Protection ◽  
Antigenic Load ◽  
Chemotactic Gradient

Abstract Tissue resident memory CD8+ T cells (Trm) are poised for immediate reactivation at sites of pathogen entry and provide optimal protection of mucosal surfaces. The intestinal tract represents a portal of entry for many infectious agents; however, to date specific strategies to enhance Trm responses at this site are lacking. Here, we present TMDI (Transient Microbiota Depletion-boosted Immunization), an approach that leverages antibiotic treatment to temporarily restrain microbiota-mediated colonization resistance, and favor intestinal expansion to high densities of an orally-delivered Listeria monocytogenes strain carrying an antigen of choice. By augmenting the local chemotactic gradient as well as the antigenic load, this procedure generates a highly expanded pool of functional, antigen-specific intestinal Trm, ultimately enhancing protection against infectious re-challenge in mice. We propose that TMDI is a useful model to dissect the requirements for optimal Trm responses in the intestine, and also a potential platform to devise novel mucosal vaccination approaches.

scholarly journals The Management of two Cases of “Spontaneous” Factor VIII Inhibitors with high Dose Cyclophosphamide

1977 ◽  
Author(s):  
E.D. Gomperts
Keyword(s):  
Factor Viii ◽  
White Male ◽  
Rational Approach ◽  
White Female ◽  
High Dose ◽  
Excessive Bleeding ◽  
Antigenic Stimulus ◽  
Inhibitor Level ◽  
Fresh Plasma ◽  
Antigenic Load

Two patients with excessive bleeding associated with “spontaneous” Factor VIII inhibitors were studied. The first, a White male aged 52 years, was treated with high dosage cyclophosphamide and steroids together with plasmapheresis. An antigenic stimulus via concentrate and fresh plasma was given together with the stat dose of cyclophosphamide (1.6gm) together with prednisolone 60mg/day. Repeat plasmopheresis was carried out on two subsequent occasions shortly thereafter. The inhibitor level dropped progressively from 6.6 u/ml to almost unrecordable levels. However, escape from control was associated with the onset of hepatitis. Further therapy with an identical form of treatment failed to subsequently modify the inhibitor level which rose progressively to very high levels. In the second case, a White female aged 79 years, plasmapheresis was not carried out (inhibitor level 2.0 u/ml). Cyclophosphamide and prednisone were given in doses of 20 mg/Kg and 60 mg/day respectively. A Factor VIII antigenic load was given 24 hours before the cyclosphosphamide. Two subsequent cyclophosphamide pulses of similar dosage were given at approximately 10 day intervals without an antigenic stimulus. The patient was then maintained on a small daily dose of cyclophosphamide (50 mg/day). The inhibitor level responded to this therapy resulting in disappearance of the inhibitor and a progressive rise in the Factor VIII to levels greater than normal (170%). Our experience with these two cases suggests that a more rational approach to immunotherapy in the second case resulted in a sustained satisfactory immunosuppressive response.

scholarly journals Complex T Cell Memory Repertoires Participate in Recall Responses at Extremes of Antigenic Load

2006 ◽  
Vol 177 (3) ◽  
pp. 2006-2014 ◽  
Author(s):  
Yuri N. Naumov ◽  
Elena N. Naumova ◽  
Shalyn C. Clute ◽  
Levi B. Watkin ◽  
Kalyani Kota ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Memory ◽  
Cell Memory ◽  
Antigenic Load

scholarly journals Chronic Helicobacter pylori Infection Does Not Significantly Alter the Microbiota of the Murine Stomach

2006 ◽  
Vol 73 (3) ◽  
pp. 1010-1013 ◽  
Author(s):  
Mai Ping Tan ◽  
Maria Kaparakis ◽  
Maja Galic ◽  
John Pedersen ◽  
Martin Pearse ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Microbial Composition ◽  
H Pylori ◽  
The Impact ◽  
Gastric Microbiota ◽  
Antigenic Load ◽  
Restriction Fragment Length

ABSTRACT We examined the impact of Helicobacter pylori infection on the murine gastric microbiota by culture and terminal-restriction fragment length polymorphism and found that neither acute nor chronic H. pylori infection substantially affected the gastric microbial composition. Interestingly, the total H. pylori burden detected by real-time PCR was significantly higher than that revealed by viable counts, suggesting that the antigenic load sustaining H. pylori-induced gastritis could be considerably higher than previously believed.

Antigenic load and peripheral chimeric levels in entire and partial liver allograft recipients

Microsurgery ◽  
2001 ◽  
Vol 21 (4) ◽  
pp. 183-187 ◽  
Author(s):  
Junhong Sun ◽  
Guang Qin ◽  
Lujia Wu ◽  
Chuanmin Wang ◽  
A.G. Ross Sheil
Keyword(s):  
Liver Allograft ◽  
Antigenic Load

scholarly journals Inflamm-ageing and lifelong antigenic load as major determinants of ageing rate and longevity

FEBS Letters ◽  
2005 ◽  
Vol 579 (10) ◽  
pp. 2035-2039 ◽  
Author(s):  
Massimo De Martinis ◽  
Claudio Franceschi ◽  
Daniela Monti ◽  
Lia Ginaldi
Keyword(s):  
Ageing Rate ◽  
Antigenic Load
Sign in / Sign up

Export Citation Format

Share Document