scholarly journals Feeding of cats with hyper-immune eggs: an oral immunization protocol v1 (protocols.io.bjnnkmde)

protocols.io ◽  
2020 ◽  
Author(s):  
Angel Justiz
Keyword(s):  
Oral Immunization ◽  
Immunization Protocol

scholarly journals Oral immunization against enterotoxigenic colibacillosis in weaned piglets by non-pathogenic Escherichia colistrain with K88 (F4) colonizing factors 

2012 ◽  
Vol 50 (No. 7) ◽  
pp. 315-320 ◽  
Author(s):  
P. Alexa ◽  
J. Hamrik ◽  
K. Stouracova ◽  
E. Salajka
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Oral Immunization ◽  
Coli Strain ◽  
Intramuscular Administration ◽  
Weaned Piglets ◽  
E Coli ◽  
Immunization Protocol ◽  
The Third ◽  
Live Culture

Experiments were focused on the prevention of diarrhoea in weaned piglets by means of enterotoxigenic strains of Escherichia coli (ETEC) with colonizing factors K88 (F4). The process of immunization consisted of intramuscular administration of ETEC strain bacterin one day prior to weaning and oral administration of a live culture of non-pathogenic E. coli strain containing colonizing factors (O149:K88; STa–, LT–) in 3 hours after weaning. The shedding of the K88 positive E. coli strains was monitored for 3 weeks after weaning by the culture of rectal swabs. The efficacy of such immunization protocol was tested by challenge exposure to enterotoxigenic E. coli O149:K88, LT+ strain on the third or the tenth day after weaning. Following the oral administration of non-pathogenic E. coli strain containing colonizing factors K88 to piglets, the shedding of the administered strain continued for 9 days. No or very small protection against diarrhoea following the challenge exposure to enterotoxigenic E. coli was found in immunized piglets.

METHOD OF DETERMINING THE IMMUNOGENIC ACTIVITY OF LIVE VACCINE AGAINST RABIES IN WILD ANIMALS IN MICE

2020 ◽  
pp. 53-57
Author(s):  
V. A. Babak ◽  
A. A. Gusev ◽  
I. A. Puntus ◽  
A. S. Smailova
Keyword(s):  
Infection Control ◽  
Rabies Virus ◽  
Experimental Infection ◽  
Oral Immunization ◽  
Live Vaccine ◽  
Wild Animals ◽  
Method Of Evaluation

The results of alternative studies on the immunogenic activity of live rabies vaccines for oral immunization of wild carnivorous animals are presented. The method of evaluation of immunogenicity using a model of oral immunization in mice with experimental infection control rabies virus CVS in the dose of 10–100 MLD50/0,03 ml. Once entered immunizing dose for white mice, weighing 12–14 g were 56.200 MLD50, the titers of VNA ranged from 1:6 to 1:16 (3,0–4,0 log2) and above.

Oral Immunization

BMJ ◽  
1948 ◽  
Vol 2 (4585) ◽  
pp. 906-906
Author(s):  
L. P. Garrod
Keyword(s):  
Oral Immunization

scholarly journals Induction of specific transplantation immunity by oral immunization with allogeneic cells

Immunology ◽  
2000 ◽  
Vol 101 (3) ◽  
pp. 404-411 ◽  
Author(s):  
V. Holan ◽  
A. Zajicova ◽  
M. Krulova ◽  
J. Plskova ◽  
J. Fric ◽  
...  
Keyword(s):  
Oral Immunization ◽  
Transplantation Immunity

scholarly journals Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg

2005 ◽  
Vol 86 (3) ◽  
pp. 601-610 ◽  
Author(s):  
Xiao-Wen He ◽  
Fang Wang ◽  
Lei Jiang ◽  
Jun Li ◽  
Shan-kui Liu ◽  
...  
Keyword(s):  
Single Dose ◽  
Plasmid Dna ◽  
Dna Vaccines ◽  
Oral Immunization ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Pcr Analysis ◽  
Target Antigen ◽  
Dna Encoding ◽  
Plga Microparticles

The purpose of this work was to assess the ability of plasmid DNA encoding hepatitis B virus (HBV) HBsAg encapsulated in poly(dl-lactide-co-glycolic acid) (PLGA) microparticles to induce local and systemic HBsAg-specific immunity following a single dose of oral immunization. RT-PCR analysis demonstrated prolonged transcription of plasmid DNA, consistent with the sustained expression and presentation of target antigen observed by confocal laser scanning microscopy, in gut-associated lymphocyte tissue (GALT) from mice immunized orally with plasmid DNA encapsulated into PLGA microparticles. Oral administration of PLGA-DNA microparticles induced a long-lasting and stable antigen-specific antibody response, both serum total antibody and intestinal IgA, in BALB/c mice. Mice immunized orally exhibited antigen-specific gamma interferon production and cytotoxic T lymphocyte responses in spleen and GALT after restimulation in vitro with HBsAg or tumour cells stably expressing HBsAg. In contrast, naked DNA vaccines given by intramuscular injection induced only systemic cellular and humoral responses to HBsAg, which were much lower than the responses elicited by oral DNA encapsulated in PLGA microparticles at equivalent doses. The results are encouraging with regard to obtaining good compliance and vaccination coverage with candidate plasmid DNA vaccines, especially in developing countries.

scholarly journals Mice Orally Immunized with a Transgenic Plant Expressing the Glycoprotein of Crimean-Congo Hemorrhagic Fever Virus

2011 ◽  
Vol 18 (12) ◽  
pp. 2031-2037 ◽  
Author(s):  
S. M. Ghiasi ◽  
A. H. Salmanian ◽  
S. Chinikar ◽  
S. Zakeri
Keyword(s):  
Transgenic Plants ◽  
Transgenic Plant ◽  
Disease Transmission ◽  
Clinical Symptoms ◽  
Hemorrhagic Fever ◽  
Oral Immunization ◽  
Negative Control ◽  
Significant Rise ◽  
Knockout Mouse Model ◽  
Crimean Congo Hemorrhagic Fever

ABSTRACTWhile Crimean-Congo hemorrhagic fever (CCHF) has a high mortality rate in humans, the associated virus (CCHFV) does not induce clinical symptoms in animals, but animals play an important role in disease transmission to humans. Our aim in this study was to examine the immunogenicity of the CCHFV glycoprotein when expressed in the root and leaf of transgenic plants via hairy roots and stable transformation of tobacco plants, respectively. After confirmatory analyses of transgenic plant lines and quantification of the expressed glycoprotein, mice were either fed with the transgenic leaves or roots, fed the transgenic plant material and injected subcutaneously with the plant-made CCHFV glycoprotein (fed/boosted), vaccinated with an attenuated CCHF vaccine (positive control), or received no treatment (negative control). All immunized groups had a consistent rise in anti-glycoprotein IgG and IgA antibodies in their serum and feces, respectively. The mice in the fed/boosted group showed a significant rise in specific IgG antibodies after a single boost. Our results imply that oral immunization of animals with edible materials from transgenic plants is feasible, and further assessments are under way. In addition, while the study of CCHF is challenging, our protocol should be further used to study CCHFV infection in the knockout mouse model and virus neutralization assays in biosafety level 4 laboratories.

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