scholarly journals Biocontrol Potential of Some Entomopathogenic Fungal Strains Against Bean Aphid Megoura japonica (Matsumura)

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 114
Author(s):  
Duy Nam Trinh ◽  
Thi Kim Lien Ha ◽  
Dewen Qiu
Keyword(s):  
Beauveria Bassiana ◽  
Culture Filtrate ◽  
Post Treatment ◽  
Verticillium Lecanii ◽  
Fungal Strains ◽  
Virulence Potential ◽  
Conidial Concentration ◽  
Biocontrol Potential ◽  
Binary Combination

This research reported the in vitro pathogenicity of Verticillium lecanii strains, L2 and L5, and Beauveria bassiana strains, B76 and B252, against Megoura japonica using leaf-dip method. The virulence potential of these four entomopathogenic fungal strains of V. lecanii and of B. bassiana were compared between fungi conidia (concentrations 1 × 106, 1 × 107, and 1 × 108 conidia mL−1) and culture filtrate. Moreover, binary combination of four different fungal strains (L2 + B76, B76 + L5, L2 + B252, and L2 + B76 + B252 + L5) were evaluated against M. japonica under control condition. Aphid mortality was recorded after two, four, six, and eight days of post-treatment. In the conidial concentration bioassay, strain B76 showed maximal mortality (85.3%) against bean aphid, and strain L5 showed the lowest effect (60.0%) at the highest concentration (1 × 108 conidia mL−1) at eight days post-treatment. Whereas, binary combinations of B76 and L2 strains showed the highest effect against M. japonica (90.5%) than other combinations. Moreover, in comparison with the effect of filtrate and conidia bioassay, 91.4% and 84.1% were achieved in strain B76, and the lowest effect (63.8% and 55.1%) was recorded in strain L5.

scholarly journals In vitro Efficacy of Verticillium lecanii and Beauveria bassiana of Commercial Source Against Cattle Tick, Rhipicephalus (Boophilus) annulatus

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Shawky Mohamed Aboelhadid ◽  
Samar Mahmoud Ibrahium ◽  
Walid Mahmoud Arafa ◽  
Lilian Nagy Maahrous ◽  
Abdel-Azeem Shaban Abdel-Baki ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Cattle Tick ◽  
Verticillium Lecanii ◽  
Boophilus Annulatus ◽  
Commercial Source

scholarly journals The influence of chitin synthesis inhibitors on growth of entomopathogenic fungi in vitro

2014 ◽  
Vol 27 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Anna Sapieha ◽  
Ryszard Miętkiewski
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Verticillium Lecanii ◽  
Chitin Synthesis ◽  
Chitin Synthesis Inhibitors ◽  
Paecilomyces Farinosus

<i>Beauveria bassiana, Paecilomyces farinosus, Verticillium lecanii, Conidiobolus thnmboides, Erynia pieris, E. radicans</i> were examined on medium to which were added three chitin synthesis inhibitors.

scholarly journals Late effects of Beauveria bassiana on larval stages of Aedes aegypti Linneo, 1762 (Diptera: Culicidae)

2022 ◽  
Vol 82 ◽  
Author(s):  
I. Quintero-Zapata ◽  
M. S. Flores-González ◽  
E. J. Luna-Santillana ◽  
N. Arroyo-González ◽  
F. L. Gandarilla-Pacheco
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Post Treatment ◽  
World Population ◽  
Larval Stages ◽  
Significant Difference ◽  
Third Stage ◽  
Close Relationship ◽  
Conidial Concentration ◽  
Plastic Containers

Abstract Aedes aegypti is a culicide that has gained relevance over the years due to its ability to transmit various viruses that cause diseases in humans that all the years cause high mortality rates in the world population. The main problem is that Ae. aegypti has managed to establish and maintain a close relationship with humans and their habitat, which is why the search for alternatives to control vector populations becomes imperative. The objective of the present work was to study the effects of two Beauveria bassiana strains on Aedes aegypti. Third instar larvae of Ae. aegypti in 250 mL plastic containers were inoculated with the GHA and NB3 strains at different concentrations (1.5 × 104, 1.5× 105, 1.5 × 106 and 1.5 × 107 conidia/mL). The NB3 strain presented highest mortality values with 63% in the highest concentration i.e., 1.5 × 107, while for the GHA strain the highest mortality value was 30.7% at the same concentration. The results showed significant difference in mortality with respect to the strain and days post treatment (P = 0.0001), but not with respect to the conidial concentration (P = 0.634). The average mortality of larvae per day for the NB3 for different concentrations ranged from 20 to 25 larvae per day, while for the GHA daily mortality ranged from 5 to 12 larvae. In post-treatment mortality, the highest mortality was recorded in the third stage larvae for the NB3, while for GHA the highest percentage mortality was observed in individuals who managed to reach the adult state. The findings of the current research depicted the noteworthy role of B. bassiana for the management of an important vector of human disease.

scholarly journals Evaluating the biocontrol potential of Canadian strain Bacillus velezensis 1B-23 via its surfactin production at various pHs and temperatures

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Michelle S. M. Li ◽  
David A. Piccoli ◽  
Tim McDowell ◽  
Jacqueline MacDonald ◽  
Justin Renaud ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Control Plant ◽  
Bacterial Disease ◽  
Bacterial Strains ◽  
Bacillus Velezensis ◽  
Fungal Strains ◽  
Biocontrol Potential ◽  
Surfactin Production

Abstract Background Microorganisms, including Bacillus species are used to help control plant pathogens, thereby reducing reliance on synthetic pesticides in agriculture. Bacillus velezensis strain 1B-23 has been shown to reduce symptoms of bacterial disease caused by Clavibacter michiganensis subsp. michiganensis in greenhouse-grown tomatoes, with in vitro studies implicating the lipopeptide surfactin as a key antimicrobial. While surfactin is known to be effective against many bacterial pathogens, it is inhibitory to a smaller proportion of fungi which nonetheless cause the majority of crop diseases. In addition, knowledge of optimal conditions for surfactin production in B. velezensis is lacking. Results Here, B. velezensis 1B-23 was shown to inhibit in vitro growth of 10 fungal strains including Candida albicans, Cochliobolus carbonum, Cryptococcus neoformans, Cylindrocarpon destructans Fusarium oxysporum, Fusarium solani, Monilinia fructicola, and Rhizoctonia solani, as well as two strains of C. michiganensis michiganensis. Three of the fungal strains (C. carbonum, C. neoformans, and M. fructicola) and the bacterial strains were also inhibited by purified surfactin (surfactin C, or [Leu7] surfactin C15) from B. velezensis 1B-23. Optimal surfactin production occurred in vitro at a relatively low temperature (16 °C) and a slightly acidic pH of 6.0. In addition to surfactin, B. velenzensis also produced macrolactins, cyclic dipeptides and minor amounts of iturins which could be responsible for the bioactivity against fungal strains which were not inhibited by purified surfactin C. Conclusions Our study indicates that B. velezensis 1B-23 has potential as a biocontrol agent against both bacterial and fungal pathogens, and may be particularly useful in slightly acidic soils of cooler climates.

scholarly journals In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by Trichoderma longibrachiatum

2021 ◽  
Vol 7 (6) ◽  
pp. 428
Author(s):  
Men Thi Ngo ◽  
Minh Van Nguyen ◽  
Jae Woo Han ◽  
Myung Soo Park ◽  
Hun Kim ◽  
...  
Keyword(s):  
Late Blight ◽  
Culture Filtrate ◽  
Fungal Pathogens ◽  
Gray Mold ◽  
Trichoderma Longibrachiatum ◽  
Chemical Structures ◽  
Natural Agents ◽  
Blight Disease

In the search for antifungal agents from marine resources, we recently found that the culture filtrate of Trichoderma longibrachiatum SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successively extracted with ethyl acetate and n-butanol to identify the fungicidal metabolites. Consequently, a new compound, spirosorbicillinol D (1), and a new natural compound, 2′,3′-dihydro-epoxysorbicillinol (2), together with 11 known compounds (3–13), were obtained from the solvent extracts. The chemical structures were determined by spectroscopic analyses and comparison with literature values. The results of the in vitro antifungal assay showed that of the tested fungal pathogens, Phytophthora infestans was the fungus most sensitive to the isolated compounds, with MIC values ranging from 6.3 to 400 µg/mL, except for trichotetronine (9) and trichodimerol (10). When tomato plants were treated with the representative compounds (4, 6, 7, and 11), bisvertinolone (6) strongly reduced the development of tomato late blight disease compared to the untreated control. Taken together, our results revealed that the culture filtrate of T. longibrachiatum SFC100166 and its metabolites could be useful sources for the development of new natural agents to control late blight caused by P. infestans.

scholarly journals Virulence of Aerial Conidia of Beauveria bassiana Produced under LED Light to Ctenocephalides felis (Cat Flea)

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Sarayut Pittarate ◽  
Malee Thungrabeab ◽  
Supamit Mekchay ◽  
Patcharin Krutmuang
Keyword(s):  
Beauveria Bassiana ◽  
Adult Mortality ◽  
Ctenocephalides Felis ◽  
Fluorescent Light ◽  
Malt Extract ◽  
Led Light ◽  
Red Led ◽  
Aerial Conidia ◽  
Different Color

Ctenocephalides felis is an ectoparasitic flea species commonly found on dogs and cats. The current study verified the in vitro virulence of conidia of the entomopathogenic fungus Beauveria bassiana produced under different color LED light (red, blue, purple, green, yellow, and white) to adults of C. felis. The fungal isolates were cultivated on malt extract agar (MEA). Bioassay treatments used aerial conidia in test tubes. Adult fleas were obtained from a house cat in Chiang Mai province, Thailand. The experiments were composed of one control and eleven treatment groups. All of the treatments with B. bassiana conidia caused adult mortality after an exposure of 12 h. Among the conditions used in this study, B. bassiana cultured under red LED and fluorescent light were the most effective in causing mortality (100 %) in adult fleas after 36 h. The experimental results indicate that these aerial conidia of B. bassiana have promising potential for use in control of C. felis adult stages.

scholarly journals Non-invasive plasma glycomic and metabolic biomarkers of post-treatment control of HIV

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Leila B. Giron ◽  
Clovis S. Palmer ◽  
Qin Liu ◽  
Xiangfan Yin ◽  
Emmanouil Papasavvas ◽  
...  
Keyword(s):  
Treatment Interruption ◽  
Post Treatment ◽  
Machine Learning Algorithms ◽  
Virologic Suppression ◽  
Hiv Latency ◽  
Viral Rebound ◽  
Treatment Control ◽  
Non Invasive ◽  
Metabolic Biomarkers

AbstractNon-invasive biomarkers that predict HIV remission after antiretroviral therapy (ART) interruption are urgently needed. Such biomarkers can improve the safety of analytic treatment interruption (ATI) and provide mechanistic insights into the host pathways involved in post-ART HIV control. Here we report plasma glycomic and metabolic signatures of time-to-viral-rebound and probability-of-viral-remission using samples from two independent cohorts. These samples include a large number of post-treatment controllers, a rare population demonstrating sustained virologic suppression after ART-cessation. These signatures remain significant after adjusting for key demographic and clinical confounders. We also report mechanistic links between some of these biomarkers and HIV latency reactivation and/or myeloid inflammation in vitro. Finally, machine learning algorithms, based on selected sets of these biomarkers, predict time-to-viral-rebound with 74% capacity and probability-of-viral-remission with 97.5% capacity. In summary, we report non-invasive plasma biomarkers, with potential functional significance, that predict both the duration and probability of HIV remission after treatment interruption.

In vitro transcriptomes analysis identifies some special genes involved in pathogenicity difference of the Beauveria bassiana against different insect hosts

2021 ◽  
Vol 154 ◽  
pp. 104824
Author(s):  
Jing Liu ◽  
Ziqi Ling ◽  
Jingjie Wang ◽  
Tingting Xiang ◽  
Liang Xu ◽  
...  
Keyword(s):  
Beauveria Bassiana
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