Virulence Potential, Antimicrobial Susceptibility and Phylogenetic Analysis of Corynebacterium pseudotuberculosis Isolated from Caseous Lymphadenitis in Sheep and Goats in Duhok City, Iraq

Background: Caseous lymphadenitis (CLA) is a chronic infectious bacterial disease of sheep and goats caused by corynebacterium pseudotuberculosis. Purpose: To determine the percentage of cases and procedures for treating Caseous lymphadenitis in Boer goats in Edufarm Kambing Burja. Methods: Data obtained from observations of 120 Boer goats and interviews with livestock owners. The data obtained were analyzed according to the reference literature. Result: The percentage of Caseous lymphadenitis in Boer goats was 8.33%. Conclusion: Procedure for handling and controlling Caseous lymphadenitis in Boer goats using abscess drainage procedures and vaccination.

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In Vitro Antimicrobial Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep with Caseous Lymphadenitis

Kocatepe Veterinary Journal ◽

10.30607/kvj.738662 ◽

2020 ◽

pp. 1-1

Author(s):

Ziya İLHAN

Keyword(s):

Antimicrobial Susceptibility ◽

Corynebacterium Pseudotuberculosis ◽

Caseous Lymphadenitis

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Activity of disinfectants and biofilm production of Corynebacterium pseudotuberculosis

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2013001100006 ◽

2013 ◽

Vol 33 (11) ◽

pp. 1319-1324 ◽

Cited By ~ 8

Author(s):

Maria da C.A. Sá ◽

Josir L.A. Veschi ◽

Grace B. Santos ◽

Evandro S. Amanso ◽

Samily A.S. Oliveira ◽

...

Keyword(s):

Quaternary Ammonium ◽

Biofilm Formation ◽

Corynebacterium Pseudotuberculosis ◽

Penicillin G ◽

Caseous Lymphadenitis ◽

Antimicrobial Drugs ◽

Sheep And Goats ◽

Biofilm Production ◽

And Control

To verify the occurrence of caseous lymphadenitis in sheep and goats on farms of Pernambuco, Brazil, and in animals slaughtered in two Brazilian cities (Petrolina/PE and Juazeiro/BA), and to characterize the susceptibility profile of Corynebacterium pseudotuberculosis to disinfectants and antimicrobials, and its relationship with biofilm production were the objectives of this study. 398 samples were tested for sensitivity to antimicrobial drugs, disinfectants, and biofilm production. Among the 108 samples collected on the properties, 75% were positive for C. pseudotuberculosis. Slaughterhouse samples indicated an occurrence of caseous lymphadenitis in 15.66% and 6.31% for animals slaughtered in Petrolina and Juazeiro respectively. With respect to antimicrobials, the sensitivity obtained was 100% for florfenicol and tetracycline; 99.25% for enrofloxacin, ciprofloxacin and lincomycin; 98.99% for cephalothin; 98.74% for norfloxacin and sulfazotrim; 97.74% for gentamicin; 94.22% for ampicillin; 91.71% for amoxicillin; 91.21% for penicillin G; 89.19% for neomycin and 0% for novobiocin. In analyzes with disinfectants, the efficiency for chlorhexidine was 100%, 97.20% for quaternary ammonium, 87.40% for chlorine and 84.40% for iodine. 75% of the isolates were weak or non-biofilm producers. For the consolidated biofilm, found that iodine decreased biofilm formation in 13 isolates and quaternary ammonia in 11 isolates. The reduction of the biofilm formation was observed for iodine and quaternary ammonium in consolidated biofilm formation in 33% and 28% of the isolates, respectively. The results of this study highlight the importance of establishing measures to prevent and control the disease.

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Molecular Characterization and Antibiotic Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep and Goats Suffering from Caseous Lymphadenitis

Zagazig Veterinary Journal ◽

10.21608/zvjz.2016.7826 ◽

2016 ◽

Vol 44 (1) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Abdelazeem Algammal

Keyword(s):

Molecular Characterization ◽

Antibiotic Susceptibility ◽

Corynebacterium Pseudotuberculosis ◽

Caseous Lymphadenitis ◽

Sheep And Goats

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Corynebacterium Pseudotuberculosis Secreted Antigen-Induced Specific Gamma-Interferon Production by Peripheral Blood Leukocytes: Potential Diagnostic Marker for Caseous Lymphadenitis in Sheep and Goats

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063871102300204 ◽

2011 ◽

Vol 23 (2) ◽

pp. 213-220 ◽

Cited By ~ 14

Author(s):

Miriam F. Rebouças ◽

Ricardo W. Portela ◽

Danielle D. Lima ◽

Dan Loureiro ◽

Bruno L. Bastos ◽

...

Keyword(s):

Peripheral Blood ◽

Diagnostic Marker ◽

Gamma Interferon ◽

Corynebacterium Pseudotuberculosis ◽

Caseous Lymphadenitis ◽

Interferon Production ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Sheep And Goats

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Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia

Scientific Reports ◽

10.1038/s41598-021-87428-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Nurul Diana Dzaraly ◽

Mohd Nasir Mohd Desa ◽

AbdulRahman Muthanna ◽

Siti Norbaya Masri ◽

Niazlin Mohd Taib ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Antimicrobial Susceptibility ◽

East Coast ◽

Tertiary Hospital ◽

Multidrug Resistant ◽

Peninsular Malaysia ◽

Clinical Isolates ◽

Serotype Distribution ◽

Antimicrobial Susceptibility Test ◽

Conjugate Vaccines

AbstractPilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline (37%), and trimethoprim-sulfamethoxazole (24%). Piliated isolates occurred in a proportion of 19%; 47.3% of them were multidrug-resistant (MDR) and a majority had serotype 19F. This study showed ST236 was the most predominant sequence type (ST) among piliated isolates, which was related to PMEN clone Taiwan19F-14 (CC271). In the phylogenetic analysis, the piliated isolates were grouped into three major clades supported with 100% bootstrap values. Most piliated isolates belonged to internationally disseminated clones of S. pneumoniae, but pneumococcal conjugate vaccines (PCVs) have the potential to control them.

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Resistance of Corynebacterium pseudotuberculosis in the Brazilian semiarid environment

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-4960 ◽

2018 ◽

Vol 38 (6) ◽

pp. 1091-1096 ◽

Cited By ~ 2

Author(s):

Maria C.A. Sá ◽

Samily A.S. Oliveira ◽

Edmilson M. Dantas Jr ◽

Gisele V. Gouveia ◽

João J.S. Gouveia ◽

...

Keyword(s):

Survival Rates ◽

Control Measures ◽

Corynebacterium Pseudotuberculosis ◽

Maximum Temperature ◽

Caseous Lymphadenitis ◽

Biofilm Production ◽

Brazilian Semiarid ◽

Higher Temperature ◽

Inoculum Survival

ABSTRACT: The semiarid northeast of Brazil contains a unique biome known as caatinga, with a maximum temperature of 40 ºC and a relativity humidity of 56%. The caatinga is characterized by a variety of plants, including Cereus jamacaru Dc (mandacaru), Poincianella microphylla Mart. ex G. Don (catingueira), Pilosocereus gounellei FAC Weber (xique-xique) and Mimosa tenuiflora (Willd.) Poir (jurema preta). Sheep and goat industries are economically strong in that region, despite the fact that caseous lymphadenitis is highly prevalent. The aim of the present study was to assess the survival and biofilm production of Corynebacterium pseudotuberculosis isolates in the environment and under controlled temperatures (28°C, 37°C and 42°C) under different surfaces (plants, soil, wood, wire and thorns). In addition, we investigated the effects of applying the disinfectants chlorhexidine, hypochlorite and quaternary ammonia in soil, tiles, wood and vegetation cover. Four strains of C. pseudotuberculosis were selected (two from goats and two from sheep) for inoculation according to their in vitro biofilm production. Adherence to microplates was used to assess the biofilm-forming ability of the bacteria. Lower survival rates were observed when isolates of C. pseudotuberculosis were subjected to a temperature of 42°C. In terms of caatinga biome plants, contamination of jurema-preta plants resulted in the lowest survival rates. The disinfectant quaternary ammonia promoted a lower inoculum survival in all surfaces. The disinfectants and the higher temperature contributed to the reduction of biofilm production in isolates of C. pseudotuberculosis. knowledge of these patterns is important for the establishment of disease control measures, given the questionable efficacy of the treatment and the immuno-prophylaxis of caseous lymphadenitis.

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