Caseous Lymphadenitis (CLA) Cases in Boer Goats

Background: Caseous lymphadenitis (CLA) is a chronic infectious bacterial disease of sheep and goats caused by corynebacterium pseudotuberculosis. Purpose: To determine the percentage of cases and procedures for treating Caseous lymphadenitis in Boer goats in Edufarm Kambing Burja. Methods: Data obtained from observations of 120 Boer goats and interviews with livestock owners. The data obtained were analyzed according to the reference literature. Result: The percentage of Caseous lymphadenitis in Boer goats was 8.33%. Conclusion: Procedure for handling and controlling Caseous lymphadenitis in Boer goats using abscess drainage procedures and vaccination.

Download Full-text

Activity of disinfectants and biofilm production of Corynebacterium pseudotuberculosis

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2013001100006 ◽

2013 ◽

Vol 33 (11) ◽

pp. 1319-1324 ◽

Cited By ~ 8

Author(s):

Maria da C.A. Sá ◽

Josir L.A. Veschi ◽

Grace B. Santos ◽

Evandro S. Amanso ◽

Samily A.S. Oliveira ◽

...

Keyword(s):

Quaternary Ammonium ◽

Biofilm Formation ◽

Corynebacterium Pseudotuberculosis ◽

Penicillin G ◽

Caseous Lymphadenitis ◽

Antimicrobial Drugs ◽

Sheep And Goats ◽

Biofilm Production ◽

And Control

To verify the occurrence of caseous lymphadenitis in sheep and goats on farms of Pernambuco, Brazil, and in animals slaughtered in two Brazilian cities (Petrolina/PE and Juazeiro/BA), and to characterize the susceptibility profile of Corynebacterium pseudotuberculosis to disinfectants and antimicrobials, and its relationship with biofilm production were the objectives of this study. 398 samples were tested for sensitivity to antimicrobial drugs, disinfectants, and biofilm production. Among the 108 samples collected on the properties, 75% were positive for C. pseudotuberculosis. Slaughterhouse samples indicated an occurrence of caseous lymphadenitis in 15.66% and 6.31% for animals slaughtered in Petrolina and Juazeiro respectively. With respect to antimicrobials, the sensitivity obtained was 100% for florfenicol and tetracycline; 99.25% for enrofloxacin, ciprofloxacin and lincomycin; 98.99% for cephalothin; 98.74% for norfloxacin and sulfazotrim; 97.74% for gentamicin; 94.22% for ampicillin; 91.71% for amoxicillin; 91.21% for penicillin G; 89.19% for neomycin and 0% for novobiocin. In analyzes with disinfectants, the efficiency for chlorhexidine was 100%, 97.20% for quaternary ammonium, 87.40% for chlorine and 84.40% for iodine. 75% of the isolates were weak or non-biofilm producers. For the consolidated biofilm, found that iodine decreased biofilm formation in 13 isolates and quaternary ammonia in 11 isolates. The reduction of the biofilm formation was observed for iodine and quaternary ammonium in consolidated biofilm formation in 33% and 28% of the isolates, respectively. The results of this study highlight the importance of establishing measures to prevent and control the disease.

Download Full-text

Molecular Characterization and Antibiotic Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep and Goats Suffering from Caseous Lymphadenitis

Zagazig Veterinary Journal ◽

10.21608/zvjz.2016.7826 ◽

2016 ◽

Vol 44 (1) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Abdelazeem Algammal

Keyword(s):

Molecular Characterization ◽

Antibiotic Susceptibility ◽

Corynebacterium Pseudotuberculosis ◽

Caseous Lymphadenitis ◽

Sheep And Goats

Download Full-text

Corynebacterium Pseudotuberculosis Secreted Antigen-Induced Specific Gamma-Interferon Production by Peripheral Blood Leukocytes: Potential Diagnostic Marker for Caseous Lymphadenitis in Sheep and Goats

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063871102300204 ◽

2011 ◽

Vol 23 (2) ◽

pp. 213-220 ◽

Cited By ~ 14

Author(s):

Miriam F. Rebouças ◽

Ricardo W. Portela ◽

Danielle D. Lima ◽

Dan Loureiro ◽

Bruno L. Bastos ◽

...

Keyword(s):

Peripheral Blood ◽

Diagnostic Marker ◽

Gamma Interferon ◽

Corynebacterium Pseudotuberculosis ◽

Caseous Lymphadenitis ◽

Interferon Production ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Sheep And Goats

Download Full-text

Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020

PLoS ONE ◽

10.1371/journal.pone.0252893 ◽

2021 ◽

Vol 16 (6) ◽

pp. e0252893

Author(s):

Abdelnasir Mohammed Adam Terab ◽

Ghada El Derdiri Abdel Wahab ◽

Hassan Zackaria Ali Ishag ◽

Nasereldien Altaib Hussein Khalil ◽

El Tigani Ahmed El Tigani-Asil ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Fibrous Tissue ◽

Postmortem Examination ◽

Camelus Dromedarius ◽

Abu Dhabi ◽

Corynebacterium Pseudotuberculosis ◽

Rrna Gene ◽

Bacterial Disease ◽

Caseous Lymphadenitis

Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by Corynebacterium pseudotuberculosis, which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in Camelus dromedarius. A total of 12 camels with suspected CLA in three regions of Abu Dhabi Emirate (Abu Dhabi, Al Ain and Al Dhafra), United Arab Emirate (UAE) were subjected to clinical and postmortem examinations from January 2015 to December 2020. Clinically, camels were emaciated and showed the presence of external caseous abscesses suggestive of CLA. Postmortem examination showed multiple abscesses of variable sizes with caseous material encapsulated by fibrous tissue in the liver, lungs, muscle, and lymph nodes. Following clinical and postmortem examination, blood, pus and different tissue samples were collected for subsequent analysis. Histopathological examination of all organs stained with Hematoxylin and Eosin (H&E) indicated a central caseo-necrotic core that was admixed with bacterial colonies and infiltration of chronic inflammatory cells, surrounded by a pyogenic membrane, and an outer fibrous connective tissue capsule. Bacterial culture identified the isolates of Corynebacterium pseudotuberculosis biotype ovis strain, and these isolates were shown to be sensitive to all antibiotics tested (penicillin, ampicillin, Co-trimoxazole, enrofloxacin and tetracycline). Moreover, the identity of the isolates was confirmed by partial sequencing of the 16S rRNA gene which showed a 100% identity to Corynebacterium pseudotuberculosis. Phylogenetic analysis based on 16S rRNA gene sequence clearly differentiates Corynebacterium pseudotuberculosis from other species of Corynebacterium. Briefly, this study provided the basic information for infection of Corynebacterium pseudotuberculosis in Camels and will help in controlling of this pathogen in the region.

Download Full-text

Virulence Potential, Antimicrobial Susceptibility and Phylogenetic Analysis of Corynebacterium pseudotuberculosis Isolated from Caseous Lymphadenitis in Sheep and Goats in Duhok City, Iraq

Advances in Animal and Veterinary Sciences ◽

10.17582/journal.aavs/2021/9.6.919.925 ◽

2021 ◽

Vol 9 (6) ◽

Author(s):

Rezheen F. Abdulrahman

Keyword(s):

Phylogenetic Analysis ◽

Antimicrobial Susceptibility ◽

Corynebacterium Pseudotuberculosis ◽

Caseous Lymphadenitis ◽

Sheep And Goats ◽

Virulence Potential

Download Full-text

Resistance of Corynebacterium pseudotuberculosis in the Brazilian semiarid environment

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-4960 ◽

2018 ◽

Vol 38 (6) ◽

pp. 1091-1096 ◽

Cited By ~ 2

Author(s):

Maria C.A. Sá ◽

Samily A.S. Oliveira ◽

Edmilson M. Dantas Jr ◽

Gisele V. Gouveia ◽

João J.S. Gouveia ◽

...

Keyword(s):

Survival Rates ◽

Control Measures ◽

Corynebacterium Pseudotuberculosis ◽

Maximum Temperature ◽

Caseous Lymphadenitis ◽

Biofilm Production ◽

Brazilian Semiarid ◽

Higher Temperature ◽

Inoculum Survival

ABSTRACT: The semiarid northeast of Brazil contains a unique biome known as caatinga, with a maximum temperature of 40 ºC and a relativity humidity of 56%. The caatinga is characterized by a variety of plants, including Cereus jamacaru Dc (mandacaru), Poincianella microphylla Mart. ex G. Don (catingueira), Pilosocereus gounellei FAC Weber (xique-xique) and Mimosa tenuiflora (Willd.) Poir (jurema preta). Sheep and goat industries are economically strong in that region, despite the fact that caseous lymphadenitis is highly prevalent. The aim of the present study was to assess the survival and biofilm production of Corynebacterium pseudotuberculosis isolates in the environment and under controlled temperatures (28°C, 37°C and 42°C) under different surfaces (plants, soil, wood, wire and thorns). In addition, we investigated the effects of applying the disinfectants chlorhexidine, hypochlorite and quaternary ammonia in soil, tiles, wood and vegetation cover. Four strains of C. pseudotuberculosis were selected (two from goats and two from sheep) for inoculation according to their in vitro biofilm production. Adherence to microplates was used to assess the biofilm-forming ability of the bacteria. Lower survival rates were observed when isolates of C. pseudotuberculosis were subjected to a temperature of 42°C. In terms of caatinga biome plants, contamination of jurema-preta plants resulted in the lowest survival rates. The disinfectant quaternary ammonia promoted a lower inoculum survival in all surfaces. The disinfectants and the higher temperature contributed to the reduction of biofilm production in isolates of C. pseudotuberculosis. knowledge of these patterns is important for the establishment of disease control measures, given the questionable efficacy of the treatment and the immuno-prophylaxis of caseous lymphadenitis.

Download Full-text