Mycological analysis of homemade and industrialized peanut seeds (Arachis hypogaea) sold in Fortaleza, Ceará

2016 ◽  
Vol 2 ◽  
pp. 134-140
Author(s):  
Larissa Nobre Veras ◽  
Erivan de Olivindo Cavalcante ◽  
Jacqueline Moura Barbosa ◽  
Thyra Pimentel Alves ◽  
José Mauro da Silva Alves ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Arachis Hypogaea ◽  
Aspergillus Flavus ◽  
Aspergillus Terreus ◽  
Penicillium Sp ◽  
Rhizopus Sp

O amendoim é uma oleaginosa originária da América do Sul. A espécie utilizada na alimentação humana, Arachis hypogaea, é bastante comercializada na forma industrializada ou caseira. Este estudo visou avaliar a contaminação micológica de amostras de amendoim industrializados e caseiros, determinando as possíveis interferências e os malefícios para a saúde humana. Para a realização do presente estudo, obteve-se a coleta de três amostras industrializadas, comercializadas em supermercados, e três amostras caseiras, vendidas por ambulantes, ambas encontradas na cidade de Fortaleza, Ceará. Elas foram levadas ao Laboratório de Microbiologia na Universidade Estadual do Ceará e os procedimentos para análise micológica foram realizados pelo método de plaqueamento. Todas as amostras analisadas em triplicada, tanto caseiras quanto industrializadas, apresentaram crescimento fúngico, constatando-se, em geral, a presença dos seguintes gêneros e espécies: Aspergillus flavus, Aspergillus terreus, Aspergillus niger, Rhizopus sp. e Penicillium sp. Os achados fúngicos podem estar relacionados a micoses, além da produção de aflatoxinas prejudiciais à saúde humana. Concluiu-se que os amendoins analisados apresentaram uma ampla contaminação fúngica, podendo ser responsáveis pelo comprometimento da saúde dos consumidores. Consequentemente, aconselha-se um maior controle nos processos de manipulação e armazenamento de amendoins, objetivando a garantia da segurança alimentar da população.

Mycoflora in Commercial Pet Foods

2001 ◽  
Vol 64 (5) ◽  
pp. 741-743 ◽  
Author(s):  
DANTE J. BUENO ◽  
JULIO O. SILVA ◽  
GUILLERMO OLIVER
Keyword(s):  
Aspergillus Niger ◽  
Moisture Content ◽  
Aspergillus Flavus ◽  
Aspergillus Terreus ◽  
Animal Health ◽  
Mold Growth ◽  
Mucor Racemosus ◽  
Isolation Frequency ◽  
Mycelia Sterilia ◽  
Made In

This article reports on the identification of mycoflora of 21 dry pet foods (12 belonging to dogs and 9 to cats) that corresponded to 8 commercial brands made in Argentina and imported. The isolation frequency and relative density of the prevalent fungal genera are compared too. Ten genera and fungi classified as Mycelia sterilia were identified. The predominant genera were Aspergillus (62%), Rhizopus (48%), and Mucor (38%). The most prevalent among Aspergillus was Aspergillus flavus followed by Aspergillus niger and Aspergillus terreus. The predominant Mucor was Mucor racemosus followed by Mucor plumbeus and Mucor globosus. The moisture content of these foods ranged from 5.6 to 10.0% and from 7.2 to 9.9% for dog and cat foods, respectively. A greater moisture content in food for the senior category (9.5 ± 0.2) was observed only in comparison to adult and kitten/puppy. If the moisture content can be maintained at these levels, mold growth would be prevented or at least it would remain at an insignificant level. Some genera and species isolated and identified from the foods analyzed are potentially producing toxins, which are known as mycotoxins. This involves a risk for animal health.

scholarly journals Hongos asociados al material particulado, en Ocaña Norte de Santander

2017 ◽  
Vol 12 (1) ◽  
pp. 75-83
Author(s):  
Jose Arnoldo Granadillo-Cuello ◽  
Alexander Armesto-Arenas ◽  
Juan Carlos Hernández-Criado ◽  
Jorge Andrés Duarte ◽  
Mayerly Pedraza-Felizola
Keyword(s):  
Aspergillus Niger ◽  
Penicillium Sp ◽  
Rhizopus Sp ◽  
La Red

El objetivo de este trabajo fue caracterizar los hongos presentes en el material particulado como indicador de riesgo de enfermedades respiratorias en Ocaña norte de Santander.  Para esto se muestrearon tres filtros extraídos de cada una de las estaciones de la red de monitoreo UFPSO-CORPONOR, se tomaron 5 puntos al azar dentro de cada filtro con dimensiones de 1cm2 y  se inocularon en medio de cultivo agar papa dextrosa (PDA) a 20°C durante 15 días, Los resultados mostraron la presencia de   3  géneros de hongos microscópicos: Penicillium sp, Aspergillus níger, Rhizopus sp y hongos unicelulares como las levaduras, que tienen una gran incidencia en enfermedades respiratorias  tales como: neumonías,  micosis sistémicas, hipersensibilidad y micotoxicosis.

scholarly journals Detection the antifungal effect of zirconium oxide nanoparticles on mold which isolated from domestic's bathroom

2018 ◽  
Vol 29 (1) ◽  
pp. 15
Author(s):  
Dina Yousif Mohamed
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Zirconium Oxide ◽  
Fungal Growth ◽  
Oxide Nanoparticles ◽  
Curvularia Lunata ◽  
Antifungal Effect ◽  
Aspergillus Brasiliensis ◽  
Rhizopus Sp ◽  
Zirconium Oxide Nanoparticles

The aim of this study is to detection the antifungal effect of Zirconium oxide nanoparticles on mold which isolated from domestic's bathroom during April 2017 in Baghdad City. Twenty species were isolated from one hundred samples which were Aspergillus niger, Aspergillus. flavus, , Aspergillus duricaulis, Aspergillus nidulans Aspergillus. parasiticus ,Aspergillus fumigatus, Aspergillus. brasiliensis ,Aspergillus heteromorphus , Curvularia lunata, Penicillium sp., Fusarium oxysporum, , Alternaria alternate, Cladosporium sp. Trichoderma sp., Mucor, Rhizopus sp., Rhodotorula mucilaginosa, , Stachybotrys and yeast. Among the isolated species Aspergillus niger, was the most abundant (14.92%) followed by Aspergillus flavus (10.14%), while less abundant (0.95 & 0.63 %) Cladosporium sp. &, Mucor respectively. The higher demoralization of fungal growth was recorded at 8mg/ml concentration of Zirconium oxide nanoparticles which was (3.8cm) in molds Aspergillus niger, Aspergillus. flavus, , & Aspergillus. brasiliensis

scholarly journals DETECTION AND IN-VITRO MANAGEMENT OF SEED BORNE MYCOFLORA ASSOCIATED WITH SUNFLOWER AND ZINNIA

2017 ◽  
Vol 29 (1) ◽  
pp. 07
Author(s):  
Gulshan Irshad ◽  
Hira Gazal ◽  
Farah Naz ◽  
Imran Hassan ◽  
Amir Bashir ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Fusarium Solani ◽  
Fusarium Moniliforme ◽  
Germination Percentage ◽  
Seedling Mortality ◽  
Control Seed ◽  
Blotter Paper ◽  
Rhizopus Sp

The present study was conducted to detect seed borne mycoflora associated with seven cultivars of sunflower (Helianthus annuus L.) and five cultivars of Zinnia (Zinnia elegans L.) by using Agar and Blotter paper methods. A total 12 genera of fungi were detected including; Alternaria alternata, Aspergillus flavus, Aspergillus niger, Cladosporium sp., Stemphylium helianthi, Penicillium sp., Fusarium oxysporum, Mucor sp., Fusarium moniliforme, Fusarium solani, Rhizocotonia solani and Rhizopus sp. The detected fungi depleted germination  10-30%, 10-20% and seedling mortality 10-17%, 10-12%. in sunflower and Zinnia respectively. A comparative study was designed to evaluate three  fungicides to control  seed mycoflora at the rate of of 1.5g/kg, 2.0g/kg, 2.5g/kg, 3.0g/kg, Maximum germination percentage was attained in case of seed dressing with Topsin M-70  followed by Bayton and Dithane M-45 at the rate of 3.0g/kg. 

scholarly journals ATIVIDADE “IN VITRO” DE TRÊS AGENTES QUÍMICOS FRENTE A DIFERENTES ESPÉCIES DE ASPERGILLUS

2007 ◽  
Vol 74 (1) ◽  
pp. 49-53
Author(s):  
M.O. Xavier ◽  
I.M. Madrid ◽  
A.R.M. Meinerz ◽  
M.B. Cleff ◽  
L.F.D. Schuch ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Aspergillus Terreus

RESUMO A aspergilose é causada por fungos ubíquos e oportunistas do gênero Aspergillus, que liberam milhares de conídios no ar, contaminando o ambiente, sendo de extrema importância a utilização de filtros de ar e programas corretos de anti-sepsia e desinfecção para prevenção da enfermidade. Este estudo avaliou a eficácia “in vitro” dos agentes químicos, iodóforo, amônia quaternária e clorexidina, frente a isolados de Aspergillus fumigatus (8), Aspergillus niger (8), Aspergillus flavus (6) e Aspergillus terreus (1). Para o teste foram preparadas diluições sucessivas dos desinfetantes/antisépticos (log2) em caldo RPMI, e os inóculos foram ajustados até uma concentração final de 5 x 104 UFC/mL. Foi realizada a técnica de microdiluição em caldo de acordo com NCCLS M-38, adaptada para agentes químicos, com incubação das microplacas a 35° C em agitação constante. A leitura visual dos resultados foi realizada após 96 horas, e os isolados de Aspergillus spp. utilizados foram resistentes ao iodóforo nas concentrações testadas. A amônia quaternária e a clorexidina mostraram-se eficazes contra os isolados de Aspergillus spp., com exceção de um A. fumigatus e um A. terreus. Com estes resultados indica-se a utilização da amônia quaternária e da clorexidina na prevenção da aspergilose, questionando-se o uso de iodóforos para este fim.

scholarly journals Sanity and Germination of Treated with Clove Extract Jurema-Preta and Faveleira Seeds

2021 ◽  
pp. 33-42
Author(s):  
Gilvanete Silva Henrique ◽  
Gilvan José Campelo Dos Santos ◽  
Jaltiery Bezerra De Souza ◽  
Mellina Nicácio Luz ◽  
Maria Alaine Cunha Lima ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Common Species ◽  
Germination Percentage ◽  
Dormancy Breaking ◽  
Forage Production ◽  
Hydroalcoholic Extract ◽  
Clove Extract ◽  
Rhizopus Sp ◽  
Aspergillus Candidus

The Mimosa tenuiflora (jurema-preta) and Cnidoscolus quercifolius (faveleira) are quite common species in the Caatinga biome, being used from forage production to energy generation and in the recovery of degraded areas for reforestation purposes, among other uses. Considering the need and importance of studies related to forest seeds health, especially native seeds and taking into account the scarcity of studies in the literature regarding the association of pathogens to the seeds of the species studied, this work aimed to evaluate the efficiency of the hydroalcoholic extract of clove on germination and incidence of fungi associated with seeds of jurema-preta and faveleira. The experiment was conducted in the Laboratory of Forest Pathology, Center of Health and Rural Technology, Federal University of Campina Grande, Patos, Paraíba, Brasil. For the germination test we performed the dormancy breaking of seeds that were then treated with clove plant extract, and as substrate, washed and sterilized sand was used. Germination percentage and Twinning speed index (SVI) were evaluated. The sanity test was performed using the filter paper method "Blotter Test" for the development of microorganisms. The treatments consisted of: 0%, 25%, 50%, 75% and 100% of clove extract, with 4 repetitions of 25 seeds. The design used was entirely randomized and the means were compared using Tukey's test at 5% probability. The clove extract, in higher concentrations, provided an increase in germination and SVI of the species. It was identified in the seeds of jurema-preta, the fungi Aspergillus niger, Aspergillus flavus, Aspergillus glaucous, Rhizopus sp, the genus Phoma sp. In the seeds of C. quercifolius the microflorea was composed by fungi Aspergillus niger, Aspergillus flavus, Aspergillus glaucous, Rhizopus sp, Aspergillus alutaceous and Aspergillus candidus.

scholarly journals Fungal Organisms Associated with Post-harvest Rot of Frafra Potatoes [Solenostemon rotundifolius (Poir.)] in Bongo-Soe, Upper East Region, Ghana

2019 ◽  
pp. 1-5
Author(s):  
S. Apuri ◽  
C. Kwoseh ◽  
E. A. Seweh ◽  
I. O. Tutu
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Colletotrichum Gloeosporioides ◽  
Curvularia Lunata ◽  
Pathology Laboratory ◽  
Post Harvest ◽  
Penicillium Sp ◽  
East Region ◽  
Upper East Region ◽  
Percentage Incidence

Aims: The study aims to identifying the microorganisms associated with post-harvest rot of frafra potatoes in Bongo-soe, Upper east region of Ghana. Place and Duration of Study: Department of Horticulture and the Pathology laboratory of the Faculty of Agriculture, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. The Solenostemon rotundifolius tubers were stored at the Horticulture Department laboratory whiles rot identification was carried out at the Pathology laboratory of the Faculty of Agriculture. The Tubers were stored from 2nd November, 2012 to 22nd March 2013. Methodology: Four hundred (400) tubers of black cultivar and four hundred (400) tubers of a brown cultivar of Solenostemon rotundifolius tubers showing visible signs of rot during the storage were collected. Pieces of diseased tissues from the margin of the necrotic collected and immersed in 10% commercial bleach solution for sterilisation, for one minute. These were then blotted dry and plated on Potato Dextrose Agar PDA. The plates were sealed with a cellotape until growth occurred. Results: The microorganisms identified to be responsible for causing rot in Solenostemon rotundifolius tubers were six in number. Colletotrichum gloeosporioides was identified to be responsible for 30.76% of rots observed, followed by Aspergillus niger, 23.07%, Curvularia lunata, 19.23%, Aspergillus flavus, 11.54%, Trichoderma sp and Penicillium sp both recorded 7.70% of rots observed. The percentage incidence of Aspergillus niger (15.38%), Curvularia lunata (11.54%) and Aspergillus flavus (7.69%) was higher in the black cultivar as compared with the brown cultivar which had percentage incidence of 7.69%, 7.69% and 3.85% respectively. Also, the percentage incidence of Colletotrichum gloeosporioides (15.38%) and Penicillium sp (3.85%) was the same in both the black and brown cultivars of Solenostemon rotundifolius tubers used in this study. Conclusion: The activities of the damaging microorganisms can be reduced by controlling mechanical injury during harvesting, transportation and storage of Solenostemon rotundifolius tubers should be prevented or reduced because they pave the way for tuber infection by the rot causing microorganisms.

scholarly journals Microorganismos potencialmente fitopatógenos en aguas de riego proveniente de la cuenca media del rio Bogotá

Nova ◽  
2018 ◽  
Vol 16 (29) ◽  
pp. 71-89
Author(s):  
Lucía Constanza Corrales Ramírez ◽  
Ligia Consuelo Sánchez Leal ◽  
Melco Esteban Quimbayo Salamanca
Keyword(s):  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Penicillium Sp ◽  
Fusarium Sp ◽  
Rhizopus Sp ◽  
Del Rio

Objetivo. Identificar microorganismos fitopatógenos presentes en las aguas utilizadas para riego en la cuenca media del rio Bogotá. Métodos. Las muestras fueron tomadas en doce fincas de la cuenca media y analizadas con la técnica de filtración por membrana. Las bacterias se identificaron por bioquímica y los hongos por claves dicotomas. Resultados. El agua de riego analizada contenía bacterias como: E.coli, P. vulgaris, A. urinae, E. cloacae, B.cereus, S. marcescens, B. brevis, y en cuanto a los hongos se encontraron: Aspergillus fumigatus, Aspergillus flavus., Mucor sp., Rhizopus sp., Penicillium sp. y Fusarium sp. La presencia de estos potenciales fitopatógenos en el agua indican un alto riesgo de contaminación y dispersión de éstos en los cultivos.

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