scholarly journals Detection of human papillomavirus type 18 oncogen E7 in patients with prostate cancer

2019 ◽  
Vol 18 (1) ◽  
pp. 37-41 ◽  
Author(s):  
G. M. Volgareva ◽  
V. D. Ermilova
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Incidence Rates ◽  
Human Papillomaviruses ◽  
Chain Reaction ◽  
National Medical ◽  
Homogeneous Cell ◽  
The Subject ◽  
Polymerase Chain ◽  
The Given

Introduction . High prostate cancer (PC) incidence rates testify to importance of research into genesis of the given disease and means of its prevention. The item of oncogenic human papillomaviruses (HPVs) participance in PC origination is still being the subject of debates. If association of PC with HPV is proven prophylaxis of the given disease becomes possible by means of inoculating boys with the vaccines made for cervical cancer (CC) prevention.Objective: to test whether prostate tissues surgically removed from PC patients harbor oncogen E7 of HPV18 – the second most common HPV type responsible for CC.Materials and methods . The study was carried out on prostate glands of 17 PC patients surgically treated in N.N. Blokhin National Medical Research Center of Oncology. Detection of HPV18 oncogen E7 was done by polymerase chain reaction. To elevate polymerase chain reaction sensitivity DNA was isolated from homogeneous cell populations collected by means of microdissections from cryopreserved PC specimens.Results . Amplification products corresponding to HPV18 oncogen E7 were registered in tests from 2 PC patients.Conclusion . HPV18 oncogen E7 was detected in surgically removed prostate tissues of 2 PC patients. In the aggregate with our previous result (7 HPV16-positive PC cases in the same group of 17 patients) the given result enables one to presume participance of oncogenic HPVs in PC genesis. The problem deserves further study.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of human papillomavirus type 16 oncogen E7 in surgical materials from Russian prostate cancer patients

2017 ◽  
Vol 16 (3) ◽  
pp. 59-62 ◽  
Author(s):  
G. M. Volgareva ◽  
V. D. Ermilova ◽  
A. V. Khachaturyan ◽  
V. V. Tatarskiy ◽  
L. S. Pavlova
Keyword(s):  
Prostate Cancer ◽  
Cervical Cancer ◽  
High Speed ◽  
Human Papillomaviruses ◽  
Chain Reaction ◽  
Dna Preservation ◽  
Human Papillomavirus Type 16 ◽  
Incidence And Mortality ◽  
Polymerase Chain ◽  
E7 Oncogene

Introduction. High indices of prostate cancer (PC) incidence and mortality as well as high speed of growth of these figures testify to urgency of research into PC origin as well as means of its prophylaxis. The problem of possible PC association with oncogenic human papillomaviruses (HPV) is still being disputable. Objective: to test whether surgical materials from PC patients in Russia harbour E7 oncogene of HPV type 16 (HPV16), the main HPV type responsible for cervical cancer. Materials and methods. Prostate tissues excised in the course of radical prostatectomy from 17 PC patients were tested by polymerase chain reaction. For better DNA preservation cryopreserved tumor specimens not treated with either formalin or paraffin were used. The PC typical multifocal type of growth was taken into account by microdissecting of cryostate cuts to accumulate homogeneous cells (cancerous, dysplastic or normal). Results. HPV16 E7 was registered in prostate tissues of 7 patients out of 17 examined including all those 5 cases for which DNA had been isolated from homogeneous sites of cancer cells. Conclusion. The result obtained enables one to admit that HPV16 may be harbored in prostates of Russian PC patients not infrequently.

Detection of legionella bacteria in sewage by polymerase chain reaction and standard culture method

1995 ◽  
Vol 31 (5-6) ◽  
pp. 409-416 ◽  
Author(s):  
Bruce M. Roll ◽  
Roger S. Fujioka
Keyword(s):  
Polymerase Chain Reaction ◽  
Sewage Treatment ◽  
Culture Method ◽  
Chain Reaction ◽  
Standard Culture ◽  
Pontiac Fever ◽  
Legionella Species ◽  
Legionnaires Disease ◽  
The Subject ◽  
Polymerase Chain

Legionella bacteria are ubiquitous in environmental waters. Only a few species of Legionella , especially, L. pneumophila are pathogenic to humans and cause a sometimes fatal Legionnaires disease as well as a less fatal disease called Pontiac fever. The presence of Legionella in sewage and aerosolized sewage is the subject of this investigation because reuse of sewage may involve the exposure of people to aerosolization, the mode of transmission of Legionella bacteria. The objective of this study was to determine the prevalence of Legionella species and L. pneumophila in wastewater and their fate after various stages of treatment. The polymerase chain reaction (PCR) and standard culture method were utilized to detect Legionella species and L. pneumophila. PCR results indicated that Legionella species were present at levels > 103 cells / ml during all phases of sewage treatment including chlorinated effluents. Culture results indicated levels at least one log lower than seen with PCR. Legionella species were also recovered from air samples collected from secondary aeration basins at levels < 103 cells/ml. PCR was shown to be the most rapid and sensitive method for detecting Legionella in sewage.

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