scholarly journals Prospects for predicting the course of nodular sclerosis Hodgkin lymphoma by morphometric analysis of CD163-positive macrophages

2021 ◽  
Vol 16 (1) ◽  
pp. 47-53
Author(s):  
M. S. Minaev ◽  
E. A. Perfilova ◽  
D. A. Diakonov ◽  
A. A. Kuzmin ◽  
N. B. Pavlova ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Morphological Analysis ◽  
Risk Groups ◽  
Test Material ◽  
Disease Course ◽  
Nodular Sclerosis ◽  
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Background. In present days, much attention is paid to the study of the interrelation between the macrophage/hystiocytic microenvironment and the tumor substrate in lymphoproliferative disorders.Objective. The article is devoted to the morphometric and morphological assessment of CD163-positive macrophages in nodular sclerosis Hodgkin lymphoma.Materials and methods. Formalin fixed, paraffin-embedded (FFPE) lymph node samples of 45 patients were used for the study. To identify and visualize CD163-positive cells in the test material, an immunohistochemical staining method was used.Results. The study shows that the morphometric and morphological analysis of CD163-positive cells can be an effective and promising criterion for representing them as potential predictors of the disease course. Immunohistochemical study of 45 cases using the CD163 marker revealed a difference in the nature of macrophages localization in the lymph nodes nodules. The dependence of CD163-expressing cells number on the disease course was determined.Conclusion. The data obtained can be used to stratify patients with nodular sclerosis of classical Hodgkin lymphoma into risk groups and to determine personalized approaches to treatment. Immunohistochemical determination of the CD163 marker can be used in the complex diagnosis of the causes of refractoriness to the first and subsequent lines of therapy.

Determination of ABO genotypes with DNA extracted from formalin-fixed, paraffin-embedded tissues

1994 ◽  
Vol 106 (6) ◽  
pp. 285-287 ◽  
Author(s):  
Mitsuko Yamada ◽  
Yoshio Yamamoto ◽  
Akio Tanegashima ◽  
Masateru Kane ◽  
Yuzuru Ikehara ◽  
...  
Keyword(s):  
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Abo Genotypes

scholarly journals Determination of Ki-67 defined growth fraction by monoclonal antibody MIB-i in formalin-fixed, paraffin-embedded prostatic cancer tissues

The Prostate ◽  
1995 ◽  
Vol 27 (3) ◽  
pp. 154-159 ◽  
Author(s):  
Marinus A. Noordzij ◽  
Theodorus H. van Der Kwast ◽  
Gert Jan van Steenbrugge ◽  
Wytske M. van Weerden ◽  
Maria H. A. Oomen ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Prostatic Cancer ◽  
Growth Fraction ◽  
Ki 67 ◽  
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Cancer Tissues ◽  
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scholarly journals Technical Validation of a Reverse-Transcription Quantitative Polymerase Chain Reaction In Vitro Diagnostic Test for the Determination of MiR-31-3p Expression Levels in Formalin-Fixed Paraffin-Embedded Metastatic Colorectal Cancer Tumor Specimens

2018 ◽  
Vol 13 ◽  
pp. 117727191876335 ◽  
Author(s):  
Lucas Ramon ◽  
Catherine David ◽  
Karine Fontaine ◽  
Elodie Lallet ◽  
Charles Marcaillou ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
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MiR-31-3p expression has been shown to be a predictive biomarker for response to anti-epithelial growth factor receptor therapy in patients with RAS wild-type metastatic colorectal cancer (mCRC). To aid in the quantification of miR-31-3p expression in formalin-fixed paraffin-embedded (FFPE) primary tumor samples from patients with mCRC, a reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay was developed and validated. Assay development included the identification of a microRNA reference standard and the determination of an appropriate relative quantification cutoff for differentiating low versus high miR-31-3p expression. Sample specimens for the validation studies included both FFPE slides and shavings. Polymerase chain reaction (PCR) efficiency and linearity, analytical sensitivity and specificity, assay robustness, reproducibility, and accuracy were demonstrated across a number of test conditions and differing quantitative PCR platforms. The data from this study provide evidence as to the feasibility of quantifying the expression of miR-31-3p from FFPE tumor tissue using a standardized RT-qPCR assay.

Diagnosis of Helicobacter pylori infection and determination of clarithromycin resistance by fluorescence in situ hybridization from formalin-fixed, paraffin-embedded gastric biopsy specimens

2005 ◽  
Vol 51 (7) ◽  
pp. 569-573 ◽  
Author(s):  
Fusun Can ◽  
Zerrin Yilmaz ◽  
Muge Demirbilek ◽  
Banu Bilezikci ◽  
Ganiye Kunefeci ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Test Method ◽  
Biopsy Specimens ◽  
Clarithromycin Resistance ◽  
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H Pylori ◽  
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A reliable diagnostic test for Helicobacter pylori is important in clinical practice and research. The ideal diagnostic test for H. pylori should be sensitive, specific, and cost-effective. Helicobacter pylori resistance to clarithromycin is a common reason for failure of eradication therapy. The aim of this study was to evaluate the fluorescent in situ hybridization (FISH) method to detect H. pylori and determine clarithromycin resistance in formalin-fixed, paraffin-embedded gastric biopsy specimens. One hundred seventeen gastric biopsy specimens from patients with dyspepsia were examined for the presence of H. pylori by conventional culture, FISH, and histopathological methods. A set of fluorescent-labeled oligonucleotide probes binding to either H. pylori 16S rRNA or 23S rRNA sequences were used for FISH analysis. Phenotypic antibiotic susceptibilities of the isolates were tested using the Epsilometer test method (E test). Helicobacter pylori was detected in 70 of 117 biopsy specimens by histopathological examination and FISH, whereas it was detected in 47 specimens by culturing. Histopathology and FISH techniques failed to identify H. pylori in 1 biopsy sample isolated by culture. Clarithromycin resistance was found in 11 of 46 H. pylori isolates using the E test method. All of the phenotypic resistance measurements of isolates were correlated with genotypic clarithromycin resistance. Eleven clarithromycin-resistant strains were identified by FISH. The diagnosis of H. pylori infection and the determination of clarithromycin resistance in formalin-fixed, paraffin-embedded specimens using FISH is promising because it provides a rapid, reliable, and culture-independent diagnosis.Key words: Helicobacter pylori, clarithromycin resistance, FISH.

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