STATISTICAL MULTIVARIATE ANALYSIS OF MELON SHAPE: A CASE STUDY USING NEAR ISOGENIC LINES

Subject. This article explores the issues of business tourism clustering in Greater Moscow. Objectives. The article intends to justify the need to create a business tourism cluster in Greater Moscow to improve the investment climate in the region. Methods. For the study, I used a multivariate analysis, forecasting, and extrapolation. Results. The article shows a certain relationship between the efficient functioning of the business tourism cluster and the economy's development. Conclusions and Relevance. Certain types of tourist clusters can serve as platforms for attracting investors and implementing marketing plans. The business tourism cluster is a link between buyers and sellers in various industries. The results of the study can be used to improve the effectiveness of the cluster initiative in business tourism, as well as find ways of cooperation between the State and private investors when creating the business tourism cluster in Greater Moscow.

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Algorithm of combining chromatography mass spectrometry-untargeted profiling and multivariate analysis for identification of marker-substances in samples of complex composition

Industrial laboratory Diagnostics of materials ◽

10.26896/1028-6861-2020-86-7-12-19 ◽

2020 ◽

Vol 86 (7) ◽

pp. 12-19

Author(s):

I. V. Plyushchenko ◽

D. G. Shakhmatov ◽

I. A. Rodin

Keyword(s):

Mass Spectrometry ◽

Multivariate Analysis ◽

Large Scale ◽

Complex Composition ◽

Unified Protocol ◽

Chromatography Mass Spectrometry ◽

Marker Substances ◽

Selection Testing ◽

Untargeted Profiling

A viral development of statistical data processing, computing capabilities, chromatography-mass spectrometry, and omics technologies (technologies based on the achievements of genomics, transcriptomics, proteomics, metabolomics) in recent decades has not led to formation of a unified protocol for untargeted profiling. Systematic errors reduce the reproducibility and reliability of the obtained results, and at the same time hinder consolidation and analysis of data gained in large-scale multi-day experiments. We propose an algorithm for conducting omics profiling to identify potential markers in the samples of complex composition and present the case study of urine samples obtained from different clinical groups of patients. Profiling was carried out by the method of liquid chromatography mass spectrometry. The markers were selected using methods of multivariate analysis including machine learning and feature selection. Testing of the approach was performed using an independent dataset by clustering and projection on principal components.

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Major Quantitative Trait Loci Mapping for Tassel Branch Number and Construction of qTBN5 Near-isogenic Lines in Maize (Zea mays L.)

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2018.01127 ◽

2018 ◽

Vol 44 (8) ◽

pp. 1127

Author(s):

Zi-Ju DAI ◽

Xin-Tao WANG ◽

Qing YANG ◽

Yan WANG ◽

Ying-Ying ZHANG ◽

...

Keyword(s):

Zea Mays ◽

Quantitative Trait Loci ◽

Quantitative Trait ◽

Zea Mays L ◽

Quantitative Trait Loci Mapping ◽

Near Isogenic Lines ◽

Branch Number ◽

Isogenic Lines ◽

Tassel Branch Number ◽

Trait Loci

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Inheritance of Stripe Rust Resistance among Near‐Isogenic Lines of Spring Wheat

Crop Science ◽

10.2135/cropsci1988.0011183x002800010012x ◽

1988 ◽

Vol 28 (1) ◽

pp. 48-54 ◽

Cited By ~ 4

Author(s):

C. A. Griffey ◽

R. E. Allan

Keyword(s):

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Near Isogenic Lines ◽

Isogenic Lines

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Normal vs. Okra Leaf Yield Interactions in Cotton. I. Performance of Near‐Isogenic Lines from Bulk Populations 1

Crop Science ◽

10.2135/cropsci1986.0011183x002600020001x ◽

1986 ◽

Vol 26 (2) ◽

pp. 219-222 ◽

Cited By ~ 5

Author(s):

William R. Meredith ◽

Randy Welles

Keyword(s):

Near Isogenic Lines ◽

Isogenic Lines ◽

Leaf Yield

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Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean

The Crop Journal ◽

10.1016/j.cj.2019.04.002 ◽

2019 ◽

Vol 7 (5) ◽

pp. 651-659 ◽

Cited By ~ 1

Author(s):

Jie Wang ◽

Pengfei Zhou ◽

Xiaolei Shi ◽

Na Yang ◽

Long Yan ◽

...

Keyword(s):

Seed Protein ◽

Near Isogenic Lines ◽

Primary Metabolite ◽

Isogenic Lines

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Comparative analysis of genetic background in eight near-isogenic wheat lines with different H genes conferring resistance to Hessian fly

Genome ◽

10.1139/g10-095 ◽

2011 ◽

Vol 54 (1) ◽

pp. 81-89 ◽

Cited By ~ 8

Author(s):

S. S. Xu ◽

C. G. Chu ◽

M. O. Harris ◽

C. E. Williams

Keyword(s):

Genetic Background ◽

Genetic Similarity ◽

Triticum Aestivum L ◽

Hessian Fly ◽

Pedigree Analysis ◽

Recurrent Parent ◽

Linkage Drag ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

H Gene

Near-isogenic lines (NILs) are useful for plant genetic and genomic studies. However, the strength of conclusions from such studies depends on the similarity of the NILs’ genetic backgrounds. In this study, we investigated the genetic similarity for a set of NILs developed in the 1990s to study gene-for-gene interactions between wheat ( Triticum aestivum L.) and the Hessian fly ( Mayetiola destructor (Say)), an important pest of wheat. Each of the eight NILs carries a single H resistance gene and was created by successive backcrossing for two to six generations to susceptible T. aestivum ‘Newton’. We generated 256 target region amplification polymorphism (TRAP) markers and used them to calculate genetic similarity, expressed by the Nei and Li (NL) coefficient. Six of the NILs (H3, H5, H6, H9, H11, and H13) had the highly uniform genetic background of Newton, with NL coefficients from 0.97 to 0.99. However, genotypes with H10 or H12 were less similar to Newton, with NL coefficients of 0.86 and 0.93, respectively. Cluster analysis based on NL coefficients and pedigree analysis showed that the genetic similarity between each of the NILs and Newton was affected by both the number of backcrosses and the genetic similarity between Newton and the H gene donors. We thus generated an equation to predict the number of required backcrosses, given varying similarity of donor and recurrent parent. We also investigated whether the genetic residues of the donor parents that remained in the NILs were related to linkage drag. By using a complete set of ‘Chinese Spring’ nullisomic-tetrasomic lines, one third of the TRAP markers that showed polymorphism between the NILs and Newton were assigned to a specific chromosome. All of the assigned markers were located on chromosomes other than the chromosome carrying the H gene, suggesting that the genetic residues detected in this study were not due to linkage drag. Results will aid in the development and use of near-isogenic lines for studies of the functional genomics of wheat.

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The Impact of Climate Change on the Resistance of Rice Near-Isogenic Lines with Resistance Genes Against Brown Planthopper

Rice ◽

10.1186/s12284-021-00508-6 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Yun-Hung Kuang ◽

Yu-Fu Fang ◽

Shau-Ching Lin ◽

Shin-Fu Tsai ◽

Zhi-Wei Yang ◽

...

Keyword(s):

Climate Change ◽

Resistance Genes ◽

Environmental Changes ◽

Gene Pyramiding ◽

Brown Planthopper ◽

Inhibitory Effect ◽

Near Isogenic Lines ◽

Impact Of Climate Change ◽

Isogenic Lines ◽

The Impact

Abstract Background The impact of climate change on insect resistance genes is elusive. Hence, we investigated the responses of rice near-isogenic lines (NILs) that carry resistance genes against brown planthopper (BPH) under different environmental conditions. Results We tested these NILs under three environmental settings (the atmospheric temperature with corresponding carbon dioxide at the ambient, year 2050 and year 2100) based on the Intergovernmental Panel on Climate Change prediction. Comparing between different environments, two of nine NILs that carried a single BPH-resistant gene maintained their resistance under the environmental changes, whereas two of three NILs showed gene pyramiding with two maintained BPH resistance genes despite the environmental changes. In addition, two NILs (NIL-BPH17 and NIL-BPH20) were examined in their antibiosis and antixenosis effects under these environmental changes. BPH showed different responses to these two NILs, where the inhibitory effect of NIL-BPH17 on the BPH growth and development was unaffected, while NIL-BPH20 may have lost its resistance during the environmental changes. Conclusion Our results indicate that BPH resistance genes could be affected by climate change. NIL-BPH17 has a strong inhibitory effect on BPH feeding on phloem and would be unaffected by environmental changes, while NIL-BPH20 would lose its ability during the environmental changes.

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Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Agriculture ◽

10.3390/agriculture11010064 ◽

2021 ◽

Vol 11 (1) ◽

pp. 64

Author(s):

Priyanka Dwivedi ◽

Naleeni Ramawat ◽

Gaurav Dhawan ◽

Subbaiyan Gopala Krishnan ◽

Kunnummal Kurungara Vinod ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Grain Quality ◽

Reproductive Stage ◽

Recurrent Parent ◽

Near Isogenic Lines ◽

Improvement Project ◽

Isogenic Lines ◽

Drought Tolerant ◽

Rice Improvement

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

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