INFLUENCE OF DIFFERENT SUBSTRATES ON IN VITRO INITIATION OF SOME EARLY AND LATE CULTIVARS OF GLOBE ARTICHOKE [CYNARA CARDUNCULUS L. SUBSP. SCOLYMUS (L.) HAYEK]

2007 ◽  
pp. 107-112 ◽  
Author(s):  
V. Castiglione ◽  
V. Cavallaro ◽  
I. Di Silvestro ◽  
M.G. Melilli
Keyword(s):  
Globe Artichoke ◽  
Cynara Cardunculus

scholarly journals Callogenesis optimization of some globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars based on in vivo and in vitro leaf explants

2020 ◽  
Vol 48 (4) ◽  
pp. 1873-1884
Author(s):  
Tugce OZSAN ◽  
Ahmet N. ONUS
Keyword(s):  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Raw Material ◽  
Naphthaleneacetic Acid ◽  
Globe Artichoke ◽  
Cynara Cardunculus ◽  
In Vitro Techniques

Globe artichoke’s [Cynara cardunculus var. scolymus (L.) Fiori] leaves are rich in polyphenols and due to health-promoting properties artichoke growing has been gaining interest. Optimization and development of valuable bioactive components, which are not in the standard amount in raw material can be achieved and increased with the assistance of in vitro techniques such as callus and subsequently cell suspension cultures. Therefore, in the present study in vitro callogenesis optimization of three globe artichoke cultivars was studied by using 29 different media combinations, based on basic Gamborg B5 medium supplemented with various concentrations of 1-Naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), and Kinetin. Comparisons were made on the basis of using in vivo and in vitro leaves as explant material. In the experiment several parameters such as leaf explants development (%), callus formation (%), and callus weight (g) were assessed for each related cultivar. Results revealed that having auxin: cytokinin concentrations together at enough and well-balanced, having equal amounts or 10:1 concentrations of auxin: cytokinin, concentrations in media combinations are indispensable for stimulating the callogenesis in globe artichoke. The findings of the present study clearly revealed that, there were differences among cultivars regarding callus induction by using in vivo and in vitro leaf explants while in vivo leaf explants came into prominence regarding callus formation and weights. It is assumed that the findings of the present study may play a complementary and auxiliary role in several areas such as pharmaceutical engineering of globe artichoke.

scholarly journals Comparative study on in vitro micropropagation response of seven globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars: open-pollinated cultivars vs F1 hybrids

2020 ◽  
Vol 48 (3) ◽  
pp. 1210-1220
Author(s):  
Tugce OZSAN ◽  
Ahmet N. ONUS
Keyword(s):  
In Vitro Rooting ◽  
Cultivar Differences ◽  
F1 Hybrids ◽  
Globe Artichoke ◽  
Ms Medium ◽  
Cynara Cardunculus ◽  
Mass Propagation ◽  
F1 Hybrid ◽  
In Vitro Micropropagation

Globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] growing has gained commercial importance in recent years due to its consumption as food. It has also started to attract attention in pharmaceutics. Due to globe artichoke’s stated importance, growers need large amount of pathogen-free, healthy starting materials for production. Stated material will maximize the yield while minimizing the costs. Hybrid cultivars have uniform in height and maturity and could be harvested concurrently; on the other hand, an open-pollinated cultivar would have useful potential that could be smoothly produced locally at a lower cost. In vitro micropropagation enabling these goals as it serves large scale, fast, reliable and realistic alternative method to classic propagation via offshoots. The aim of the present study was to comparatively evaluate the micropropagation efficiency of two important local open-pollinated (OP) cultivars (‘Bayrampaşa’, ‘Sakız’) and five F1 hybrid cultivars (‘Olympus’, ‘Madrigal’, ‘Sambo’, ‘Green Globe’, ‘Imparator’), on the basis of total subcultures they were subjected to. Various plant growth regulators at various combinations were assessed for in vitro micropropagation and subsequent in vitro rooting. 3/4 basic MS medium supplemented with 0.05 mg  L-1 BA + 0.005 mg L-1 IBA was determined as the best media combination for in vitro micropropagation, while 10.0 mg L-1 IAA + 1.0 g L-1 activated charcoal adding to 1/2 basic MS medium had positive effects on in vitro rooting. According to results, the micropropagation efficiency varied based on cultivar differences and number of subcultures regardless of being OP or F1 hybrid. The present study demonstrated that in vitro propagation of globe artichoke could be a valuable process for assessing mass propagation regardless of using F1 or OP cultivars. Considering the OP cultivars are cheap in terms of price in a comparison to F1 hybrid cultivars, OP cultivars could be also recommended to be used for in vitro mass propagation. 

scholarly journals The Effects of Bergamot Polyphenolic Fraction, Cynara cardunculus, and Olea europea L. Extract on Doxorubicin-Induced Cardiotoxicity

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2158
Author(s):  
Jessica Maiuolo ◽  
Irene Bava ◽  
Cristina Carresi ◽  
Micaela Gliozzi ◽  
Vincenzo Musolino ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Apoptotic Cell ◽  
Natural Compounds ◽  
Cynara Cardunculus ◽  
Unfolded Protein ◽  
Wide Range ◽  
Vitro Model ◽  
The Unfolded Protein Response ◽  
Protein Response

Doxorubicin is an anthracycline that is commonly used as a chemotherapy drug due to its cytotoxic effects. The clinical use of doxorubicin is limited due to its known cardiotoxic effects. Treatment with anthracyclines causes heart failure in 15–17% of patients, resulting in mitochondrial dysfunction, the accumulation of reactive oxygen species, intracellular calcium dysregulation, the deterioration of the cardiomyocyte structure, and apoptotic cell death. Polyphenols have a wide range of beneficial properties, and particular importance is given to Bergamot Polyphenolic Fraction; Oleuropein, one of the main polyphenolic compounds of olive oil; and Cynara cardunculus extract. These natural compounds have particular beneficial characteristics, owing to their high polyphenol contents. Among these, their antioxidant and antoproliferative properties are the most important. The aim of this paper was to investigate the effects of these three plant derivatives using an in vitro model of cardiotoxicity induced by the treatment of rat embryonic cardiomyoblasts (H9c2) with doxorubicin. The biological mechanisms involved and the crosstalk existing between the mitochondria and the endoplasmic reticulum were examined. Bergamot Polyphenolic Fraction, Oleuropein, and Cynara cardunculus extract were able to decrease the damage induced by exposure to doxorubicin. In particular, these natural compounds were found to reduce cell mortality and oxidative damage, increase the lipid content, and decrease the concentration of calcium ions that escaped from the endoplasmic reticulum. In addition, the direct involvement of this cellular organelle was demonstrated by silencing the ATF6 arm of the Unfolded Protein Response, which was activated after treatment with doxorubicin.

scholarly journals A double-blind, placebo-controlled, cross-over study to establish the bifidogenic effect of a very-long-chain inulin extracted from globe artichoke (Cynara scolymus) in healthy human subjects

2010 ◽  
Vol 104 (7) ◽  
pp. 1007-1017 ◽  
Author(s):  
Adele Costabile ◽  
Sofia Kolida ◽  
Annett Klinder ◽  
Eva Gietl ◽  
Michael Bäuerlein ◽  
...  
Keyword(s):  
Gastrointestinal Symptoms ◽  
Gas Production ◽  
Globe Artichoke ◽  
Daily Consumption ◽  
Healthy Human ◽  
Double Blind ◽  
Cynara Scolymus ◽  
The Impact ◽  
Long Chain

There is growing interest in the use of inulins as substrates for the selective growth of beneficial gut bacteria such as bifidobacteria and lactobacilli because recent studies have established that their prebiotic effect is linked to several health benefits. In the present study, the impact of a very-long-chain inulin (VLCI), derived from globe artichoke (Cynara scolymus), on the human intestinal microbiota compared with maltodextrin was determined. A double-blind, cross-over study was carried out in thirty-two healthy adults who were randomised into two groups and consumed 10 g/d of either VLCI or maltodextrin, for two 3-week study periods, separated by a 3-week washout period. Numbers of faecal bifidobacteria and lactobacilli were significantly higher upon VLCI ingestion compared with the placebo. Additionally, levels ofAtopobiumgroup significantly increased, whileBacteroides–Prevotellanumbers were significantly reduced. No significant changes in faecal SCFA concentrations were observed. There were no adverse gastrointestinal symptoms apart from a significant increase in mild and moderate bloating upon VLCI ingestion. These observations were also confirmed byin vitrogas production measurements. In conclusion, daily consumption of VLCI extracted from globe artichoke exerted a pronounced prebiotic effect on the human faecal microbiota composition and was well tolerated by all volunteers.

Development and characterization of microsatellite markers in Cynara cardunculus L.

Genome ◽  
2005 ◽  
Vol 48 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Alberto Acquadro ◽  
Ezio Portis ◽  
David Lee ◽  
Paolo Donini ◽  
Sergio Lanteri
Keyword(s):  
Molecular Markers ◽  
Fold Increase ◽  
The United States ◽  
Enriched Library ◽  
Globe Artichoke ◽  
Cynara Cardunculus ◽  
Principal Coordinates Analysis ◽  
Principal Coordinates ◽  
Novel Approach ◽  
Wild Cardoon

Cynara cardunculus L. is a species native to the Mediterranean basin that comprises 2 crops, globe artichoke (var. scolymus L.) and cultivated cardoon (var. altilis DC), as well as wild cardoon (var. sylvestris (Lamk) Fiori). Globe artichoke represents an important component of the South European agricultural economy but is also cultivated in North Africa, the Near East, South America, the United States, and China. Breeding activities and molecular marker studies have been, to date, extremely limited. Better knowledge of the genome of the species might be gained by developing a range of molecular markers. Here, we report on the development of 14 microsatellites (simple sequence repeats (SSRs)) through a novel approach that we have defined as the microsatellite amplified library (MAL). The approach represents a combination of amplified fragment length polymorphism and a primer extension based enriched library, is rapid, and requires no hybridization enrichment steps. The technique provided a ~40-fold increase in the efficiency of SSR identification compared with conventional library procedures. The developed SSRs were applied for genotyping 36 accessions of C. cardunculus, including a core of 27 varietal types of globe artichoke, 3 accessions of cultivated cardoon, and 6 Sicilian accessions of wild cardoon. Principal coordinates analysis made it possible to differentiate both cultivated and wild forms from each other.Key words: globe artichoke, wild and cultivated cardoon, molecular markers, AFLP, MAL (microsatellite amplified library).

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