MOLECULAR AND PHYTOCHEMICAL STUDIES OF PAPAVER SOMNIFERUM IN THE CONTEXT OF INFRASPECIFIC CLASSIFICATION

DNA barcoding of ITS and psbA-trnH regions, histochemistry as well as thin layer chromatography (TLC) of Morinda brevipes S.Y. Hu were analyzed. Transverse section of root revealed the presence of cortex, xylem, cork cell, stone cells, and calcium oxalate sandy crystal. The lower epidermis cells showed many stoma in paracytic or inequality type. Spiral vessel and tiny calcium oxalate needle crystal usually appeared in the powder. TLC showed the presence of emodin in M. brevipes. Phytochemical studies revealed the existence of carbohydrates, saponins, tannins, flavones, anthraquinones, alkaloids and volatile oils. The ITS and psbAtrnH sequences were found for the first time which were submitted to NCBI to obtain the GenBank registration number. This study might play an important role in the identification, and utilization of M. brevipes for various purposes.

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COMBINING ABILITY FOR INHERITANCE OF ECONOMIC TRAITS IN OPIUM POPPY (Papaver somniferum L.)

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v24i2.17000 ◽

2011 ◽

Vol 24 (2) ◽

pp. 01-08

Author(s):

B. Kumar ◽

N. K. Patra

Keyword(s):

Combining Ability ◽

Diallel Cross ◽

Papaver Somniferum ◽

Opium Poppy ◽

Economic Traits ◽

Degree Of Dominance ◽

Parental Material ◽

The Mean ◽

Per Se ◽

Important Medicinal Plant

Opium poppy (Papaver somniferum L.) is an important medicinal plant produces more than 80 alkaloids obtained from the capsules and straw of the plant. The estimate of combining ability gives an indication of the genetic behaviour of the parental material. It is therefore, desirable to select the parents for hybridization on the basis of their per se performance and combining ability effects. The F1, F2 and their reciprocals of an eight parent diallel cross in opium poppy were studied for combining ability of seven economic traits. The mean sum of squares due to GCA, SCA and reciprocals were significant for all the traits. The magnitude of GCA variances were invariably higher than those of SCA and thus indicating the preponderance of non-additive genetic variances, which was further affirmed by the measure of average degree of dominance i.e. ?(?2s/? 2g). Among the parents VN35I for plant height, Sanchita and VG26 for capsules per plant, VN23, VN35I and Vivek for capsule index, VG26 and Sanchita for seed and straw yield per plant, and VN35I and VG20 for morphine content were found good general combiners. Earliness being a desired trait, parent VG20 having significant negative GCA estimates coupled with per se performance can be considered as good general combiner for early flowering. Inclusion of good general combiners in a multiple crossing program or an inter-mating population involving all possible crosses among them subjected to bi-parental mating may be expected to offer maximum promise in breeding for economic traits.DOI: http://dx.doi.org/10.3329/bjpbg.v24i2.17000

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Phytochemical Studies And Antibacterial Investigations on Gliricidia Sepium

PARIPEX-INDIAN JOURNAL OF RESEARCH ◽

10.15373/22501991/jan2014/3 ◽

2012 ◽

Vol 3 (1) ◽

pp. 12-13 ◽

Cited By ~ 1

Author(s):

D. Sukumar D. Sukumar ◽

◽

C. Aparna C. Aparna

Keyword(s):

Gliricidia Sepium ◽

Phytochemical Studies

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Comparative Pharmacognostic, Physicochemical and Phytochemical Studies of Different Samples of Plumbago zeylanica L. Roots

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2017.090601 ◽

2017 ◽

Vol 9 (06) ◽

Author(s):

Neha Jain ◽

Mohan Lal Kori

Keyword(s):

Phytochemical Analysis ◽

Local Market ◽

Herbal Drugs ◽

Disease Treatment ◽

Organoleptic Evaluation ◽

Plumbago Zeylanica ◽

Qualitative And Quantitative ◽

Crude Drugs ◽

Phytochemical Studies

The objective of the present study is to evaluate the quality of the marketed and self collected samples of Plumbago zeylanica L. roots on the standardization parameters. This study is planned mainly to confirm changes with quality of drug. Now a day’s more demand of herbal drugs for disease treatment, lack of knowledge of proper methodology and availability are promoting the practices of adulteration and substitution. Thus, the standardization of the plant crude drugs is necessary to maintain their therapeutic efficacy. Comparative studies were carried out to evaluate the standards of P. zeylanica L. with emphasis on organoleptic evaluation, physicochemical and phytochemical analysis. Samples were procured from local market and self collected to determine the qualitative and quantitative variations. The result indicates that self collected sample showed significant results with comparison to marketed sample.

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Pharmacognostical fingerprinting and selective bioactivity studies of Solanum glaucophyllum Desf.

Journal of Traditional and Folk Practices ◽

10.25173/jtfp.2018.6.1.87 ◽

2018 ◽

Vol 6 (1) ◽

Author(s):

Anindya Sundar Ray ◽

Suman Kalyan Mandal ◽

Chowdhury Habibur Rahaman

Keyword(s):

Medicinal Plant ◽

Radical Scavenging ◽

Ethanol Extract ◽

Inhibition Zone ◽

Phytochemical Analysis ◽

Antimicrobial Studies ◽

Maximum Inhibition ◽

Crude Drugs ◽

Phytochemical Studies ◽

Dpph Radical Scavenging

Solanum glaucophyllum Desf. (Solanaceae) is traditionally used for curing several health conditions in both human and domesticated animals. There is no data available regarding pharmacognostic standardization and pharmacological activity of this less known medicinal plant. Therefore, the aim of the present work is to prepare pharmacognostic fingerprints of crude drugs obtained from the leaf and stem parts of S. glaucophyllum. Apart from this detailed phytochemical analysis, antioxidant and antimicrobial studies have also been carried out. Pharmacognostic study revealed that the leaves are amphistomatic and stomata are strictly of anisocytic type. Values of stomatal index, palisade ratio, ash content of the investigated parts are found distinct and can be used as pharmacognostic standards for evaluation of crude drugs of this medicinal plant. Phytochemical studies of the leaf and stem parts indicated that phenolics, flavonoids, tannins and alkaloids are present in quite impressible amount. Curcumin content in stem was found very high through HPLC analysis. In DPPH radical scavenging assay, the stem part showed significant antioxidant potential. Ethanol extract of stem produced maximum inhibition zone (11mm) against Pseudomonas aeruginosa at the concentration of 50 mg/ml among five test microorganisms. Some pharmacognostic features recorded in this study as diagnostic ones will help in proper identification of this ethnomedicinal plant in its fresh as well as dried form. On the other hand preliminary phytochemical, antioxidant and antimicrobial studies highlight the S. glaucophyllum as a promising candidate for bioprospecting.

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