Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksana Batang Benalu (Dendrophthoe falcata (L.f) Ettingsh)

ABSTRAK Penelitian ini adalah penelitian eksplorasi yang bertujuan untuk mengisolasi senyawa metabolit sekunder yang terdapat dalam ekstrak n- heksana batang benalu pohon kakao Dendrophthoe falcata (L.f) Ettingsh, yang berasal dari Desa Cendana, Kec. Burau, Kab. Luwu Timur Makassar, Sulawesi Selatan. Isolasi dilakukan dalam beberapa tahap yaitu maserasi, partisi dengan n-heksana, fraksinasi, uji kemurnian dan identifikasi. Hasil penelitian berupa isolat murni berbentuk kristal jarum berwarna putih dengan titik leleh 1400C. Hasil uji dengan pereaksi wagner membentuk endapan cokelat. Identifikasi dengan spektrum infra merah yang menunjukkan bilangan gelombang (cm-1) yakni: 3550,95 (N-H); 1696,36 (C=O); 1303,88 (CN), 2945,30 dan 2856,58 (CH alifatik), 883,40 (C-H aromatik), 1454,33 (C=C). Berdasarkan hasil IR dan Uji warna menunjukkan bahwa isolat diduga senyawa golongan alkaloid. Kata kunci : Isolasi Dendrophthoe falcata (L.f) Ettingsh, senyawa alkaloid ABSTRACT This study is exploratory research that aimed to isolate and identify the secondary metabolite compound contained in the n-hexane extract of D.falcata (L.f) Ettingsh from Cendana village, Burau district, Luwu Timur Regency of Makassar. Isolation is done in several stages; maceration, partitioning with n-hexane, fractionation, purification and dentification. The result was in pure needle crystal shape with a melting point of 140,2°C. The test result with the Wagner reagent test showed the formation of a brown precipited. Analyzing of IR Spectrum of Isolate showed Several wave numbers; 3550,95 (N-H); 1696,36 (C=O); 1303,88 (CN), 2945,30 dan 2856,58 (CH alifatik), 883,40 (C-H aromatik), 1454,33 (C=C). Based on the result of IR datas and reagen test it is suggest that the isolate is alkaloid compound. Keywords : Isolation, Dendrophthoe falcata (L.f) Ettingsh, alkaloid compound

Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksana Daun Kayu Jawa (Lannea coromandelica Houtt Merr.)

ABSTRAK Penelitian ini adalah penelitian eksplorasi yang bertujuan untuk mengisolasi senyawa metabolit sekunder dalam ekstrak n-heksana Daun Lannea coromandelica Houtt Merr. yang berasal dari kecamatan Cina, kabupaten Bone, Sulawesi Selatan. Isolasi dilakukan dengan beberapa tahap yaitu maserasi, partisi dengan n-heksana, fraksinasi, pemurnian dan identifikasi. Hasil penelitian diperoleh isolat murni berupa kristal jarum berwarna putih dengan titik leleh 140,20C. Hasil uji dengan pereaksi Meyer menghasilkan endapan putih dan Wagner menghasilkan endapan coklat menunjukkan positif alkaloid. Identifikasi dengan spektroskopi infra merah memberikan serapan pada bilangan gelombang (cm-1) : 3502,73 ; 2954,99 ; 2868,15 ; 1687,71; 1463,97; 1382,96; 1330,88; dan 1037,70 yang menunjukkan adanya gugus fungsi -N-H, -C-H alifatik (CH2 dan CH3), -C=O, -C=C, dan -C-N. Kata kunci : Daun L.coromandelica Houtt Merr., Isolasi, Alkaloid. ABSTRACT This study is exploratory research that aims to isolate the secondary metabolite compound contained in n-hexane extract of Lannea coromandelica Houtt Merr. leaves from Cina district, Bone Regency, South Sulawesi. The isolation was done through several stages, maceration, partitioning with n-hexane, fractionation, purification and identification. The results was obtained in pure needle crystal shape with a melting point 140,2°C. The test result with the Meyer reagent showed the formation of a white precipitated and the Wagner test showed the formation of a brown precipitated, there for it can be categorized as positive alkaloid. Identification with an infrared spectroscopy giving absorbance at the wave number (cm-1): 3502,73 ; 2954,99 ; 2868,15 ; 1687,71; 1463,97; 1382,96; 1330,88; and 1037,70 refers of function groups -N-H, -C-H aliphatic (CH2 and CH3), -C=O, -C=C, and -C-N. Keywords : L.coromandelica Houtt Merr., Leaves, Isolation, Alkaloid.

Pharmacognostic studies of Morinda brevipes S. Y. Hu

DNA barcoding of ITS and psbA-trnH regions, histochemistry as well as thin layer chromatography (TLC) of Morinda brevipes S.Y. Hu were analyzed. Transverse section of root revealed the presence of cortex, xylem, cork cell, stone cells, and calcium oxalate sandy crystal. The lower epidermis cells showed many stoma in paracytic or inequality type. Spiral vessel and tiny calcium oxalate needle crystal usually appeared in the powder. TLC showed the presence of emodin in M. brevipes. Phytochemical studies revealed the existence of carbohydrates, saponins, tannins, flavones, anthraquinones, alkaloids and volatile oils. The ITS and psbAtrnH sequences were found for the first time which were submitted to NCBI to obtain the GenBank registration number. This study might play an important role in the identification, and utilization of M. brevipes for various purposes.

Amplified Emission and Field-Effect Transistor Characteristics of One-Dimensionally Structured 2,5-Bis(4-biphenylyl)thiophene Crystals

One-dimensional (1D) structures of 2,5-bis(4-biphenylyl)thiophene (BP1T) crystals are fabricated for light amplification and field-effect transistor (FET) measurements. A strip-shaped 1D structure (10 m width) made by photolitography of a vapor-deposited polycrystalline film shows amplified spontaneous emission and lasing oscillations under optical pumping. An FET fabricated with this 1D structure exhibits hole-conduction with a mobility of h = 8.0×10−3 cm2/Vs. Another 1D-structured FET is fabricated with epitaxially grown needle-like crystals of BP1T. This needle-crystal FET exhibits higher mobility of h 034 cm2/Vs. This improved hole mobility is attributed to the single-crystal channel of epitaxial needles while the grain boudaries in the polycrystalline 1D-structure decrease the carrier transport.

ISOLASI KUMARIN DARI BIJI PINANG (Areca catechu L.)

ABSTRACT Coumarin has been isolated from seed of Areca catechu L. an ethyl acetate fraction which needle crystal products at 184 –185°C. Based on thin layer chromatography analysis using n-hexane : acetone (4 : 6) as eluent got Rf 0.6. From elemental analysis, mass spectra, ultraviolet spectra, infra red spectra, nuclear magnetic resonance spectra ( H and C NMR) ,concluded this compound was 7-hidroksi-4-metil coumarine. Keywords : Areca catechu, 7-hidroksi-4-metil kumarin.