scholarly journals Effect of erythropoietin on the content of lipid peroxidation products in lymphocytes in experimental thermal injury

2015 ◽  
Vol 96 (5) ◽  
pp. 849-853 ◽  
Author(s):  
M V Osikov ◽  
E V Simonyan ◽  
O T Saedgalina
Keyword(s):  
Lipid Peroxidation ◽  
Peripheral Blood ◽  
Thermal Injury ◽  
White Male ◽  
Peripheral Blood Lymphocytes ◽  
Lipid Extract ◽  
Male Rats ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
Blood Lymphocytes

Aim. To investigate the effect of different concentrations of erythropoietin on the content of lipid peroxidation products in lymphocytes isolated from the blood of rats with thermal injury. Methods. The study was performed on 22 white male rats. Thermal injury of IIIA degree on 4% of body surface area was simulated by immersion in water at a temperature of 98-99 °C. After 24 hours, blood lymphocytes were isolated and the content of the primary (diene conjugates), secondary (ketodienes and conjugated trienes) and final products (Schiff bases) of lipid peroxidation were determined spectrophotometrically. Erythropoietin was added to lymphocytes at concentrations of 0.01; 0.1 and 1 IU/ml. Results. It was found that 24 hours after thermal injury there were the accumulation of primary, secondary and final products of lipid peroxidation in isopropanol fraction of lipid extracts of peripheral blood lymphocytes. Addition of erythropoietin to the rat lymphocytes resulted in a controversial change in the content of lipid peroxidation products: an increase in the heptane fraction, decrease - in the isopropanol fraction of lipid extract of lymphocytes. In the heptane fraction erythropoietin (at concentrations of 0.01, 0.1, and 1 IU/ml) increased the content of primary, end (at a concentration of 0.1 IU/ml) and secondary (at a concentration of 1 IU/ml) lipid peroxidation products. In isopropanol fraction erythropoietin reduced the content of primary (at concentrations of 0.01, 0.1, and 1 IU/ml), final (at concentrations of 0.01 and 0.1 IU/ml) and secondary (at concentrations of 0.01 and 1 IU/ml) products of lipid peroxidation. Conclusion. It was found that there is an accumulation of lipid peroxidation products in the isopropanol fraction of lipid extract of lymphocytes isolated from peripheral blood of rats with thermal injury; erythropoietin application at concentrations of 0.01; 0.1 and 1 IU/ml increases the content of lipid peroxidation products in heptane fraction and decrease in the isopropanol fraction of lipid extract of lymphocytes.

scholarly journals Changes of Prooxidant-Antioxidant Systems in Experimental Acute Pancreatitis

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
Viktoria Cherkasova ◽  
Luibomyr Zaiats
Keyword(s):  
Lipid Peroxidation ◽  
Acute Pancreatitis ◽  
White Male ◽  
Thiobarbituric Acid ◽  
Activity Level ◽  
Male Rats ◽  
Antioxidant Systems ◽  
Diene Conjugates ◽  
Pronounced Increase ◽  
Oxidation Rates

Mortality in acute destructive pancreatitis, despite the development and introduction of new methods of treatment, remains stable high and in severe forms reaches 25-85%. Activation of neutrophils and macrophages in acute pancreatitis leads to an "oxygen burst", which is closely linked with the activation of lipid peroxidation.Goals. The purpose is to establish dynamic changes in the indexes of prooxidant-antioxidant systems in acute L-arginine-induced pancreatitis.Materials and methods. The study was performed on 62 white male rats of Wistar line weighing 180-220g, with modeled acute pancreatitis. Blood for analysis have been taken: the blood serum on 12, 24, 48 and 72 hours of experiment to determine the activity level of thiobarbituric acid products, diene conjugates, catalase and lactate for assessment of the intensity of oxidative stress and antioxidant systems.Results. The obtained results of the study showed that acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation processes (LPO). Revealed that the most pronounced increase in all blood parameters is observed 24 hours after the beginning of the study. A significant increase in the active products of tiobarbituric acid (TBA-AP) and diene conjugates (DC) was detected - 1.98 and 2.7 times, respectively, and 2.2 times the growth of catalase (CT). At the next stage of the experiment there is a slowdown in the rate of LPO, as evidenced by the following values. Thus, for 48 years in the 3rd group: TBA-AP - they increased by 5.1% (p> 0.05), DC - by 3.3% (p> 0.05), and the level of CT - by 43.4% (P <0.05), compared with data for 24 hours. It is important to note that at 72 hours, the CT level decreased by 23.3% (p> 0.05), which may indicate an exhaustion of antioxidant systems. Indicators of LPO on 72 hours compared with 48 hours in group III: TBA-AP - increased by 1.7% (p> 0.05), DC - by 5.7% (p> 0.05).Conclusions. Acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation-oxidation processes that can potentiate the development of multiple organ failure in pancreatic inflammation. The most pronounced changes in lipid peroxidation-oxidation rates are observed for 24 hours of study.

scholarly journals CHANGE OF IMMUNOLOGICAL RESISTANCE AND BIOCHEMICAL PROCESSES IN PERIPHERAL BLOOD LYMPHOCYTES IN ACTION STRESS

2021 ◽  
Vol 4 ◽  
pp. 8-15
Author(s):  
А. Kydyrmoldina ◽  
B. Zhetpisbayev ◽  
A. Utegenova ◽  
E. Omarkhanova ◽  
M. Malik ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Energy Metabolism ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
The State ◽  
Phase Changes ◽  
Antioxidant Systems ◽  
Biochemical Processes ◽  
Blood Lymphocytes ◽  
Muscle Stress

The aim of the study was to study changes in immunological reactivity and biochemical processes in peripheral blood lymphocytes in Pakistani students under the action of muscle stress. To achieve this goal, the states of the immune, lipid peroxidation and antioxidant systems and energy metabolism were studied in 30 students from South Asia under the influence of muscle stress. The state of cellular, humoral and nonspecific phagocytic immunity was determined in all students before and 1, 2 and 3 days after the stress of muscle load. The research results show that the state of the immune system after muscle stress was characterized by phase changes in the parameters of cellular, humoral and nonspecific phagocytic immunity with a restoration or increase in indicators at the end of the observation. An increase in the activity of enzymes of energy metabolism in peripheral blood lymphocytes after stress of muscular load on days 2 and 3 after ensures the processes of successful adaptation. The state of lipid peroxidation and the antioxidant system in peripheral blood lymphocytes under muscle stress reflects the stress of the organism's adaptive reactions with the development of lipid hyperperoxidation under conditions of increased energy costs.

Cytotoxic Effects of Curcumin At Various Concentrations and Role of Curcumin on Lipid Peroxidation and Activities of Antioxidant Enzymes of the Rat Peripheral Blood Lymphocytes

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4933-4933 ◽  
Author(s):  
Nisarg Desai ◽  
G T Finosh ◽  
N G Panicker ◽  
R Ramachandran ◽  
Alex Varghese
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Cell Viability ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Ros Scavenging ◽  
Cytotoxic Effects ◽  
Blood Lymphocytes ◽  
Scavenging Effect ◽  
Antioxidant Effects

Abstract Abstract 4933 Background: Curcumin (diferuloylmethane) is an active ingredient of turmeric and has been suggested to have antiproliferative, pro-oxidant and cytotoxic effects (Das R, Apoptosis 2008). Curcumin has also been shown to have antioxidant effects (Rastogi M, Free Radic Res 2008). Our aim was to assess the cytotoxic, pro-oxidant and antioxidant effects of curcumin on rat peripheral blood lymphocytes (RPL) in culture. Methods: Different concentrations of curcumin (0.325 μM, 0.65 μM, 1.3 μM) were analyzed for cytotoxic effects on rat peripheral lymphocytes. The cytotoxic effect of curcumin was assessed by measuring cell viability using Trypan blue exclusion assay. DNA damage by curcumin (at 0.65 μM) was confirmed by the COMET assay. The effect of curcumin (at 0.65 μM) on oxidation was assessed by measuring the activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and lipid peroxidation (LP) by spectrophotometry. RPLs were cultured for 72 hr in vitro. The pro-oxidant effect of curcumin was determined by comparing LP induction in control (PBS treated) vs curcumin treated RPLs. Parameters were measured at 0, 24 and 48 hrs of curcumin exposure. Reactive oxygen species (ROS) generated by treatment with FeCl3 (10 mM) and H2O2 (5 mM) (Fe/H) were used to demonstrate the ROS scavenging effect of curcumin. Lymphocyte cultures were treated with either Fe/H alone or in combination with Curcumin. Oxidation levels were assessed as described above at 0, 24, 48 hrs following exposure. Results: Cytotoxic effects were seen at different curcumin concentrations (Table 1). Viability of RPLs were 42% at 0.64 μM concentration vs 72% at 0.325 μM. 30% decrease in cell viability of RPLs were seen at 0.65 μM concentration compared to 0.325 μM. Hence, 0.65 μM concentration was used for further experiments. There was a significant increase in DNA damage in curcumin exposed lymphocytes at 48hr (table 2). There was a significant increase in LP in curcumin exposed RPLs compared to control lymphocytes. By contrast, there was no significant change in LP values in presence of Fe/H at all time points compared to control. There was a significant decrease in LP when RPL cultures were incubated with curcumin for 48 hr compared to 0 hr of incubation. There was significant increase in activities of SOD, catalase and GPx in presence of curcumin and Fe/H (table 3 and 4). Conclusions: Dose dependent cytotoxic and DNA damage effects of curcumin are seen in RPLs. Treatment with curcumin alone increases lipid peroxidation, However in the presence of oxidative stress, curcumin may act as an antioxidant (ROS scavenging effect) by stimulating activities of antioxidant enzyme and preventing lipid peroxidation. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Lipid peroxidation in rat cartilage under experimental osteoarthritis and administration of multiprobiotic

2020 ◽  
Vol 80 (1) ◽  
pp. 41-44
Author(s):  
O. Korotkyi ◽  
L. Kot ◽  
K. Dvorshchenko
Keyword(s):  
Lipid Peroxidation ◽  
Drinking Water ◽  
Schiff Bases ◽  
Male Rats ◽  
Intragastric Administration ◽  
Nacl Solution ◽  
Diene Conjugates ◽  
Active Compounds ◽  
Knee Ligament ◽  
Lipid Peroxidation Products

The aim of the study was to investigate the effect of multiprobiotic on the content of lipid peroxidation products in rat cartilage during monoiodoacetate-induced osteoarthritis. The study was carried out on white non-linear, sexually mature male rats (weight 180-240g), according to general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group – Control: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The second group – Multiprobiotic: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic Symbiter® (Prolisok ", Ukraine) diluted in 1 ml of drinking water per 1 kg of animal weight. The third group, MIA-induced OA: animals got injection into knee ligament 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The fourth group – MIA-induced OA + Multiprobiotic: animals got injection into knee ligament 0.05 ml of 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic diluted in 1 ml of drinking water per 1 kg of animal weight. All animals were killed on day 30 of the experiment, according to the protocol of the ethics committee with rapid blood sampling. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-dinitrophenylhydrazine. The content of diene conjugates was determined in the heptane-isopropanol extract by the spectrophotometric method, and of Schiff bases – by the fluorimetric method. The content of TBK-active compounds was determined by reaction with thiobarbituric acid. It has been established that MIA-induced OA the content of lipid peroxidation products (diene conjugates, TBK-active compounds, schiff bases) increases in the cartilage. It was shown that with the administration of multiprobiotic in animals with MIA-induced OA, the above indicators were restored.

scholarly journals Ameliorative Effect of Eugenol and Anethole on Arsenic Induced Oxidative DNA Damage in Cultured Human Peripheral Blood Lymphocytes

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Anita Yadav ◽  
Surbhi Bal ◽  
Neha Verma ◽  
Neeraj K. Aggarwal ◽  
Ranjan Gupta
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Peripheral Blood ◽  
Oxidative Dna Damage ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Tail Moment ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

Arsenic contamination is one of the major health concerns all over the world and associated with various types of cancer and pathological effects. The production of reactive oxygen species (ROS) plays a crucial role in arsenic mediated toxicity. Several studies have shown that population constantly exposed to arsenic have substantial oxidative stress that, in turn, induces DNA damage. In the present work eugenol and anethole were investigated for their protective effect against arsenic mediated oxidative DNA damage in peripheral blood lymphocytes. Comet assay and lipid peroxidation was used as biomarker of genotoxicity and oxidative stress respectively. A dose dependent increase in tail moment and lipid peroxidation was observed when lymphocytes were treated with sodium arsenite. Treatment of arsenic (50?M) along with eugenol (20?M) and anethole (50?M) showed a significant decrease in the tail moment and lipid peroxidation in cultured peripheral blood lymphocytes. The decrease in the tail moment and lipid peroxidation was significantly higher in combined supplementation of eugenol and anethole as compared to individual administration. The results of the present study suggests ameliorative role of eugenol and anethole against arsenic induced genotoxic and oxidative damage in cultured human peripheral blood lymphocytes.

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