scholarly journals Changes of Prooxidant-Antioxidant Systems in Experimental Acute Pancreatitis

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
Viktoria Cherkasova ◽  
Luibomyr Zaiats
Keyword(s):  
Lipid Peroxidation ◽  
Acute Pancreatitis ◽  
White Male ◽  
Thiobarbituric Acid ◽  
Activity Level ◽  
Male Rats ◽  
Antioxidant Systems ◽  
Diene Conjugates ◽  
Pronounced Increase ◽  
Oxidation Rates

Mortality in acute destructive pancreatitis, despite the development and introduction of new methods of treatment, remains stable high and in severe forms reaches 25-85%. Activation of neutrophils and macrophages in acute pancreatitis leads to an "oxygen burst", which is closely linked with the activation of lipid peroxidation.Goals. The purpose is to establish dynamic changes in the indexes of prooxidant-antioxidant systems in acute L-arginine-induced pancreatitis.Materials and methods. The study was performed on 62 white male rats of Wistar line weighing 180-220g, with modeled acute pancreatitis. Blood for analysis have been taken: the blood serum on 12, 24, 48 and 72 hours of experiment to determine the activity level of thiobarbituric acid products, diene conjugates, catalase and lactate for assessment of the intensity of oxidative stress and antioxidant systems.Results. The obtained results of the study showed that acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation processes (LPO). Revealed that the most pronounced increase in all blood parameters is observed 24 hours after the beginning of the study. A significant increase in the active products of tiobarbituric acid (TBA-AP) and diene conjugates (DC) was detected - 1.98 and 2.7 times, respectively, and 2.2 times the growth of catalase (CT). At the next stage of the experiment there is a slowdown in the rate of LPO, as evidenced by the following values. Thus, for 48 years in the 3rd group: TBA-AP - they increased by 5.1% (p> 0.05), DC - by 3.3% (p> 0.05), and the level of CT - by 43.4% (P <0.05), compared with data for 24 hours. It is important to note that at 72 hours, the CT level decreased by 23.3% (p> 0.05), which may indicate an exhaustion of antioxidant systems. Indicators of LPO on 72 hours compared with 48 hours in group III: TBA-AP - increased by 1.7% (p> 0.05), DC - by 5.7% (p> 0.05).Conclusions. Acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation-oxidation processes that can potentiate the development of multiple organ failure in pancreatic inflammation. The most pronounced changes in lipid peroxidation-oxidation rates are observed for 24 hours of study.

scholarly journals Effect of erythropoietin on the content of lipid peroxidation products in lymphocytes in experimental thermal injury

2015 ◽  
Vol 96 (5) ◽  
pp. 849-853 ◽  
Author(s):  
M V Osikov ◽  
E V Simonyan ◽  
O T Saedgalina
Keyword(s):  
Lipid Peroxidation ◽  
Peripheral Blood ◽  
Thermal Injury ◽  
White Male ◽  
Peripheral Blood Lymphocytes ◽  
Lipid Extract ◽  
Male Rats ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
Blood Lymphocytes

Aim. To investigate the effect of different concentrations of erythropoietin on the content of lipid peroxidation products in lymphocytes isolated from the blood of rats with thermal injury. Methods. The study was performed on 22 white male rats. Thermal injury of IIIA degree on 4% of body surface area was simulated by immersion in water at a temperature of 98-99 °C. After 24 hours, blood lymphocytes were isolated and the content of the primary (diene conjugates), secondary (ketodienes and conjugated trienes) and final products (Schiff bases) of lipid peroxidation were determined spectrophotometrically. Erythropoietin was added to lymphocytes at concentrations of 0.01; 0.1 and 1 IU/ml. Results. It was found that 24 hours after thermal injury there were the accumulation of primary, secondary and final products of lipid peroxidation in isopropanol fraction of lipid extracts of peripheral blood lymphocytes. Addition of erythropoietin to the rat lymphocytes resulted in a controversial change in the content of lipid peroxidation products: an increase in the heptane fraction, decrease - in the isopropanol fraction of lipid extract of lymphocytes. In the heptane fraction erythropoietin (at concentrations of 0.01, 0.1, and 1 IU/ml) increased the content of primary, end (at a concentration of 0.1 IU/ml) and secondary (at a concentration of 1 IU/ml) lipid peroxidation products. In isopropanol fraction erythropoietin reduced the content of primary (at concentrations of 0.01, 0.1, and 1 IU/ml), final (at concentrations of 0.01 and 0.1 IU/ml) and secondary (at concentrations of 0.01 and 1 IU/ml) products of lipid peroxidation. Conclusion. It was found that there is an accumulation of lipid peroxidation products in the isopropanol fraction of lipid extract of lymphocytes isolated from peripheral blood of rats with thermal injury; erythropoietin application at concentrations of 0.01; 0.1 and 1 IU/ml increases the content of lipid peroxidation products in heptane fraction and decrease in the isopropanol fraction of lipid extract of lymphocytes.

Expression Analysis of 4-Hydroxynonenal Modified Proteins in Schizophrenia Brain; Relevance to Involvement in Redox Dysregulation

2021 ◽  
Vol 18 ◽  
Author(s):  
Sobia Manzoor ◽  
Ayesha Khan ◽  
Beena Hasan ◽  
Shamim Mushtaq ◽  
Nikhat Ahmed
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Brain Regions ◽  
Protein Adducts ◽  
Antioxidant Systems ◽  
Control Group ◽  
Tbars Level ◽  
Elevated Expression ◽  
Modified Proteins

Background: Oxidative damage contributes to the pathophysiology of schizophrenia (SZ). Redox imbalance may lead to increased lipid peroxidation, which produces toxic aldehydes like 4-hydroxynonenal (4-HNE) ultimately leading to oxidative stress. Conversely, implications of oxidative stress points towards an alteration in HNE-protein adducts and activities of enzymatic and antioxidant systems in schizophrenia. Objectives: Present study focuses on identification of HNE-protein adducts and its related molecular consequences in schizophrenia pathology due to oxidative stress, particularly lipid peroxidation. Material and Methods: Oxyblotting was performed on seven autopsied brain samples each from cortex and hippocampus region of schizophrenia patients and their respective normal healthy controls. Additionally, thiobarbituric acid substances (TBARS), reduced glutathione (GSH) levels and catalase (CAT) activities associated with oxidative stress, were also estimated. Results: Obtained results indicates substantially higher levels of oxidative stress in schizophrenia patients than healthy control group represented by elevated expression of HNE-protein adducts. Interestingly, hippocampus region of schizophrenia brain shows increased HNE protein adducts compared to cortex. An increase in catalase activity (4.8876 ± 1.7123) whereas decrease in antioxidant GSH levels (0.213 ± 0.015µmol/ml) have been observed in SZ brain. Elevated TBARS level (0.3801 ± 0.0532ug/ml) were obtained in brain regions SZ patients compared with their controls that reflects an increased lipid peroxidation (LPO). Conclusion: Conclusion: We propose the role of HNE modified proteins possibly associated with the pathology of schizophrenia. Our data revealed increase lipid peroxidation as a consequence of increased TBARS production. Furthermore, altered cellular antioxidants pathways related to GSH and CAT also highlight the involvement of oxidative stress in schizophrenia pathology.

scholarly journals Organ-Specific Oxidative Events under Restrictive Versus Full Reperfusion Following Hemorrhagic Traumatic Shock in Rats

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2195
Author(s):  
Carina Penzenstadler ◽  
Anna Zifko ◽  
Mohammad Jafarmadar ◽  
Janin Schulte ◽  
Joachim Struck ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Thiobarbituric Acid ◽  
Traumatic Shock ◽  
Male Rats ◽  
Resonance Spectroscopy ◽  
Stress Reactions ◽  
Paramagnetic Resonance ◽  
Early Reperfusion ◽  
Ros Formation

Background aim: Reperfusion after hemorrhagic traumatic shock (HTS) is often associated with complications that are partly ascribed to the formation of reactive oxygen species (ROS). The aim of our study was to compare the effects of restrictive reperfusion (RR) to rapid full reperfusion (FR) on ROS formation and/or oxidative events. Materials and methods: Anesthetized male rats were randomly subjected to HTS followed by FR (75 mL/kg/h) or RR (30 mL/kg/h for 40 min, followed by 75 mL/kg/h) with Ringer’s solution (n = 8/group). Compartment-specific ROS formation was determined by infusion of ROS scavenger 1-hydroxy-3-carboxy-2,2,5,5-tetramethyl-pyrrolidine hydrochloride (CP-H) during resuscitation, followed by electron paramagnetic resonance spectroscopy. Sham-operated animals (n = 8) served as controls. The experiment was terminated 100 min post-shock. Results: Mean arterial pressure was significantly higher in the FR compared to the RR group during early reperfusion. Only RR animals, not FR animals, showed significantly higher ROS concentrations in erythrocytes (1951 ± 420 vs. 724 ± 75 AU) and in liver (474 ± 57 vs. 261 ± 21 AU) compared to sham controls. This was accompanied by elevated alanine aminotransferase and creatinine levels in RR animals compared to both shams and FR animals, while lipid peroxidation products (thiobarbituric acid reactive substances) were significantly increased only in the kidney in the FR group (p < 0.05). RR animals showed significantly higher plasma peroxiredoxin-4 values when compared to the FR group (20 ± 2 vs. 14 ± 0.5 RLU). Conclusion: Restrictive reperfusion after HTS is associated with increased ROS formation in erythrocytes and liver compared to sham controls. Moreover, the restrictive reperfusion is associated with a more pronounced injury to the liver and kidney, which is likely mediated by other than lipid peroxidation process and/or oxidative stress reactions.

scholarly journals Gender Peculiarities of Protein and Lipid Peroxidation of the Oral Liquid Under the Conditions of Preclinical Iodine and Iron Deficiency

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Olga Zayats
Keyword(s):  
Lipid Peroxidation ◽  
Iron Deficiency ◽  
Oral Fluid ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Endogenous Factors ◽  
Dental Pathology ◽  
Oral Liquid

The prevalence of somatic pathology in children increases significantly with each passing year, which is associated with socio-economic, environmental conditions, the complex interaction of exogenous and endogenous factors. This problem may be related to micronutrient imbalances, among which iodine deficiency and sideropenia are quite common. Objective. The aim of the study is to learn the peculiarities of peroxidation of proteins and lipids, the system of antioxidant defence of oral fluid in conditions of preclinical iodine and iron deficiency. Materials and Methods. The study included 115 children aged 6-18 years. The level of peroxidation of proteins was characterized by the content of oxidative modification of protein products (aldehyde derivatives of the main nature) in the oral fluid. Peroxidation of lipids of oral fluid was assessed by the accumulation of diene conjugates (DCs) of polyunsaturated fatty acids and products responding to thiobarbituric acid (TBA-RP). Results. It has been determined that in case of iodine and iron deficiencies, their combination causes significant activation of protein and lipid peroxidation processes in the oral fluid of children, accompanied by an imbalance of the prooxidant-antioxidant system of the oral cavity. Conclusion. Such changes can be predictors of the development of dental pathology

scholarly journals INFLUENCE OF SEMAX ON THE INTENSITY OF LIPID PEROXIDATION IN IMMUNOCOMPETENT ORGANS IN THE CONDITIONS OF “SOCIAL” STRESS

2019 ◽  
Vol 19 (1S) ◽  
pp. 188-191
Author(s):  
M A Samotrueva ◽  
A L Yasenyavskaya ◽  
V Kh Murtalieva ◽  
N F Myasoedov ◽  
L A Andreeva
Keyword(s):  
Lipid Peroxidation ◽  
Social Stress ◽  
Initial Level ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
Sensory Contact ◽  
Corrective Effect ◽  
Immunocompetent Organs ◽  
Thymus Tissue ◽  
Tissue Homogenates

This study is devoted to an experimental study of the effect of Semax (Met-Glu-His-Phe-Pro-Gly-Pro) on the intensity of lipid peroxidation of the thymus and spleen lipids of male rats under conditions of “social” stress. “Social” stress in animals was modeled by the formation of aggressive and submissive behavior of males in the conditions of distant sensory contact. The intensity of lipid peroxidation (LPO) in the thymus and spleen was determined by spectrophotometry using three indicators: the initial level of products reacting with thiobarbituric acid (TBA-reactive products), the rate of spontaneous and induced by ascorbate and iron ions of LPO. “Social” stress is accompanied by an increase in peroxidation processes in immunocompetent organs, which contributes to the development of stress-induced functional disorders of the immune system. Against the background of Semax administration under “social” stress, its pronounced corrective effect on lipid peroxidation rates is observed, as evidenced by a decrease in spleen and thymus tissue homogenates of male rats in the initial level of TBA-reactive products, as well as spontaneous and ascorbate-dependent levels of lipid peroxidation.

scholarly journals Relationship between lipid peroxidation and antioxidants in rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes

2021 ◽  
Vol 11 (6) ◽  
pp. 249-258
Author(s):  
Bоhdana Verveha
Keyword(s):  
Lipid Peroxidation ◽  
Abdominal Cavity ◽  
White Male ◽  
Male Rats ◽  
Laboratory Animals ◽  
Lipid Hydroperoxides ◽  
Tbars Level ◽  
Sod Activity ◽  
Generalized Peritonitis ◽  
Streptozotocin Induced Diabetes

Introduction. There are evidence that oxidative stress is involved in the pathogenesis of the acute generalized peritonitis and diabetes mellitus.The aim of study was to examine levels of the products of lipid peroxidation and the antioxidants in rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes.Material and methods. The experiment was performed on 66 adult white male rats weighing 220-300 g. Diabetes mellitus in experimental animals was simulated by a single intraperitoneal injection of streptozotocin (Sigma) at the dose of 60 mg/kg. Acute generalized peritonitis was induced with 10% faecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method. The thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOHs), superoxide dismutase (SOD) activity, catalase (CAT) and ceruloplasmin (CP) were estimated and compared.Results. Our results showed that during all stages of development of acute generalized peritonitis on the background of streptozotocin-induced diabetes there is the LOOHs decrease and the TBARS increase. We observed statistically significant inverse correlations between the level of LOOHs and SOD in the animals of subgroup 1 (r = -0.88; p <0.05) and inverse correlations between the TBARS level and the SOD level in the subgroup 3 of animals (r = -0.74; p <0.05). We also found a statistically significant inverse correlation between the CAT level and the TBARS level (r = -0.86; p <0.05) and between the CP level and the TBARS level in the blood of animals in the subgroup 3 (r = -0.87; p <0.05).Conclusion. Negative statistically significant correlations between the TBARS level and the antioxidants (SOD, CAT, CP) in the blood of animals with acute generalized peritonitis on the background of streptozotocin-induced diabetes indicate a predictor role of lipid peroxidation processes in the depletion of antioxidant resources

scholarly journals Показники вільнорадикального окисненняв щурів за умови “пасивного тютюнокуріння” на тлі тривалого введення глутамату натрію у статевому і віковому аспектах

2018 ◽  
Author(s):  
A. V. Rutska ◽  
I. Ya. Krynytska ◽  
M. I. Marushchak
Keyword(s):  
Lipid Peroxidation ◽  
Lung Tissue ◽  
Tobacco Smoking ◽  
Monosodium Glutamate ◽  
Nutritional Supplements ◽  
Female Rats ◽  
Male Rats ◽  
Tissue Homogenate ◽  
Prolonged Administration ◽  
Pronounced Increase

Introduction. The widespread prevalence of tobacco smoking is a global problem of humanity, the solution of which is directed at the efforts of many scientists and professionals. At the same time, the distinctive feature of modern food technologies is the use of nutritional supplements. One of the most common nutritional supplements in Ukraine and in Europe is glutamate sodium (E621), which is not always safe for human health.The aim of the study – to investigate indices of free radical oxidation in rats during “passive tobacco smoking” on the basis of prolonged administration of monosodium glutamate in the sex and age aspects.Research Methods. The study was conducted on 160 white, non-linear, sexually mature and sexually immature rats of both sexes. Each group of animals was divided into four subgroups: I – control; ІІ – rats, which were modeled “passive tobacco smoking”; ІІІ – rats, which were given glutamate of sodium; IV – rats, which were modeled "passive tobacco smoking" against the background of the introduction of glutamate sodium.Results and Discussion. Under the condition of «passive tobacco smoking» there is a pronounced increase in the TBА-active products (TBА-AP)content both in serum and in the lung tissue homogenate. In male-rats, this indicator has significantly increased by 3.3 and 4.2 times, respectively, and in female-rats – by 3.9 and 5.2 times, respectively. Given the prolonged administration of sodium glutamate in male-rats, this indicator significantly increased by 1.6 and 2.1 times, and in females-rats by 19.6 % and 53.9% respectively. In animals, which were modeled «passive tobacco smoking» and administered glutamate sodium, the most pronounced increase in the content of TBА-AP was established. In male-rats, this indicator has increased significantly in 4.9 and 6.8 times, and in female-rats – by 6.5 and 8.9 times, respectively.Conclusions. Under the condition of «passive tobacco smoking» intensification of peroxide oxidation of lipids (increase in the content of TBА-active products) in blood and lung tissue is observed, more pronounced under the condition of “passive tobacco smoking” against the background of the introduction of glutamate sodium. In the sexual comparison of changes in the system of lipid peroxidation, their reliable prevalence in female-rats was revealed. With the age-old comparison of changes in the system of lipid peroxidation, their reliable prevalence in immature animals has been established.

scholarly journals Role of lipid peroxidation in pathogenesis of senile cataract

2009 ◽  
Vol 66 (5) ◽  
pp. 371-375 ◽  
Author(s):  
Bojana Kisic ◽  
Dijana Miric ◽  
Lepsa Zoric ◽  
Ilija Dragojevic ◽  
Aleksandra Stolic
Keyword(s):  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
Mature Cataract ◽  
Structure Changes ◽  
Biochemical Research ◽  
Maturity Degree ◽  
Optical Change

Background /Aim. Cataract is a structural, biochemical and optical change in the eye lens, which changes transmission and refraction of light rays reducing keenness and clarity of a figure on the retina. Its occurrence is highest in older people, over the age of 65 (45.9%), thus a certain degree of opacification exists practically in all people over the 70. Our research was directed to measuring of lipid peroxidation products in cataract lenses involved in early stages of cataractogenesis through oxidative stress and in the development of mature cataract. Methods. Clinical and biochemical research was carried out in 101 patients with cataract, 46 women and 55 men. The average age of the group was 72.47 (? = 7.98). According to the cataract maturity degree the patients were classified into two groups as follows: cataracta senilis incipiens (n = 41) and cataracta senilis matura (n = 60). Measuring of diene conjugates was carried out by spectrophotometer. Fluorescent lipid peroxidation products were measured by a spectrofluorophotometer, and malondialdehyde (MDA) concentration was measured by colorimeter as a product of a reaction with thiobarbituric acid (TBA). Result. Significantly higher diene conjugated concentration in lenses was measured in the patients with the diagnosis cataracta senilis incipiens (p < 0.001) as well as the intensity of fluorescent iminopropens (p < 0.001). Significantly higher MDA concentration in lens (p < 0.001) was measured in the patients with cataracta senilis matura. Conclusion. The lens structure changes caused by lipid peroxidation can, with other risk factors present, influence the occurrence and development of mature cataract. Some cataract types show different lipid peroxidation intensity with the most distinct changes in cataract which started as corticonuclear.

scholarly journals α-Tocopherol Protects the Heart, Muscles, and Testes from Lipid Peroxidation in Growing Male Rats Subjected to Physical Efforts

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Magdalena Górnicka ◽  
Anna Ciecierska ◽  
Jadwiga Hamulka ◽  
Małgorzata E. Drywień ◽  
Joanna Frackiewicz ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Experimental Period ◽  
Treadmill Running ◽  
Male Rats ◽  
Rat Tissues ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Tocopherol Concentration

The effect of α-tocopherol supplementation on adaptation to training is still equivocal. The aim of the study was to determine the effect of training and α-tocopherol supplementation on α-tocopherol and thiobarbituric acid reactive substance (TBARS) concentration in the rat liver, heart, muscles, and testes. Male Wistar rats (n=32) were divided into four groups (nonsupplemented, not trained—C; nonsupplemented, trained—CT; supplemented, not trained—E; supplemented and trained—ET). During the 14-day experimental period, 2 mg/d of vitamin E as α-tocopherol acetate was administered to the animals (groups E and ET). Rats in the training group (CT and ET) were subjected to 15 minutes of treadmill running each day. The α-tocopherol levels in rat tissues were assessed using high-performance liquid chromatography (HPLC). Lipid peroxides were determined by TBARS spectrophotometric method. α-Tocopherol had a significant impact on α-tocopherol concentration in all tissues. Training increased the α-tocopherol concentration in the heart and muscles but reduced it in the liver. Training also caused increased lipid peroxidation in the muscles, heart, and testes; but a higher α-tocopherol content in tissues reduced the TBARS level. The main finding of the study is that impaired α-tocopherol status and its adequate intake is needed to maintain optimal status to prevent damage to the skeletal and cardiac muscles as well as the testes in growing individuals.

scholarly journals Reperfusion endotoxin eye damage in experimental study

2015 ◽  
Vol 96 (5) ◽  
pp. 811-814 ◽  
Author(s):  
G A Drozdova ◽  
A F Samigullina ◽  
E A Nurgaleeva ◽  
A A Sorokin
Keyword(s):  
Lipid Peroxidation ◽  
Circulatory Arrest ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
Integral Index ◽  
Radical Oxidation ◽  
Endogenous Intoxication ◽  
Eye Tissues ◽  
Peroxidation Processes

Aim. To evaluate the level of endogenous intoxication in the blood and the status of the lipid peroxidation processes in eye tissues of experimental animals after acute circulatory arrest. Methods. Chronic (5 weeks) pathophysiological experiments were performed in 72 nonlinear mature male rats, in which acute circulatory arrest was modeled by neurovascular heart bundle compression, followed by resuscitation. Endogenous intoxication intensity was assessed using endogenous intoxication integral index based on the detection of oligopeptides and low and medium molecular weight substances. Free radical oxidation in eye tissues was assessed by determining the level of 2’-thiobarbituric acid-reactive products, as well as by chemiluminescence. The condition of antioxidant system was investigated by catalase activity features and reduced glutathione level. Results. There was a significant two-phased increase of endogenous intoxication index during the whole postoperative period. Fe2+-induced chemiluminescence with subsequent accumulation of lipid peroxidation secondary metabolites, as well as increased activity of catalase protective system both in early stages (days 1-3) and on the second week were revealed in eye tissues. A positive correlation of endogenous intoxication integral index and the level of 2’-thiobarbituric acid-reactive products on the first day after resuscitation was revealed. Conclusion. Endogenous intoxication syndrome is formed after the acute circulatory arrest with maximal levels of endogenous toxins in the systemic circulation on 1-3rd and on 10-14th day after resuscitation, as well as with significant intensification of lipid peroxidation processes in eye tissues at the same period.

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