scholarly journals Antimicrobial, Antioxidative, Elastase and Tyrosinase Inhibitory Effect of Supercritical and Hydrothermal Halopteris scoparia Extract

2021 ◽  
Vol 12 (5) ◽  
pp. 407-413
Author(s):  
Soo Hyeon Heo, Su In Park, Jinseo Lee, Sung Won Jung,Moon Sam Shin
Keyword(s):  
Propionibacterium Acnes ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Halopteris Scoparia ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

To find out a good candidate of cosmetic source and screened for antimicrobial, antioxidant and whitening activities. In the antioxidant experiment, supercritical Halopteris scoparia extraction (SHE) and hydrothermal Halopteris scoparia extraction (HHE) were slightly different depending on the experimental method (Total polyphenol content measurement, DPPH radical scavenging ability, ABTS radical scavenging ability, and SOD-like activity) and in the enzyme experiment (Elastase, Tyrosinase inhibition activity), the supercritical extract was more effective. In supercritical fluid, Halopteris scoparia extracts showed good antimicrobial activity against Propionibacterium acnes, Staphylococcus aureus, Bacillus subtilis and Escherichia coli but hydrothermal extract had no effect about the rest of the strains. Therefore, it can be expected that the supercritical Halopteris scoparia extract with excellent antibacterial activity can be used as a cosmetic material.

scholarly journals The Galloyl Catechins Contributing to Main Antioxidant Capacity of Tea Made fromCamellia sinensisin China

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Chunjian Zhao ◽  
Chunying Li ◽  
Shuaihua Liu ◽  
Lei Yang
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Epigallocatechin Gallate ◽  
Dpph Radical ◽  
Polyphenol Content ◽  
Epicatechin Gallate ◽  
Total Polyphenol ◽  
Total Polyphenol Content ◽  
Antioxidant Capacities ◽  
Dpph Radical Scavenging

Total polyphenol content, catechins content, and antioxidant capacities of green, dark, oolong, and black teas made fromCamellia sinensisin China were evaluated. The total polyphenol content of 20 samples of tea was in the range of 7.82–32.36%. Total catechins content was in the range of 4.34–24.27%. The antioxidant capacity of tea extract was determined by the oxygen radical absorbance capacity (ORAC) test and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging test. Total polyphenol content, catechins content, and antioxidant capacity decreased in the following order: green > oolong > black > dark tea. A positive correlation existed between the antioxidant capacity and total polyphenol content or catechins content (R2=0.67–0.87). The antioxidant capacities of five major catechins (epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epicatechin, epigallocatechin, and catechin) were determined by online HPLC DPPH radical-scavenging; the antioxidant activity of tea was mainly attributed to the esterified catechins (EGCG or ECG).

scholarly journals Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 592 ◽  
Author(s):  
Chengli Hou ◽  
Liguo Wu ◽  
Zhenyu Wang ◽  
Elena Saguer ◽  
Dequan Zhang
Keyword(s):  
Liquid Chromatography ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Amino Acid Sequences ◽  
Dpph Radical ◽  
Antioxidant Peptides ◽  
Animal Blood ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

scholarly journals THE CHARACTERISTICS AND ANTIOXIDANT ACTIVITIES OF CHABA MAPLE (HIBISCUS ACETOSELLA) HOMEMADE JAM

2019 ◽  
pp. 52-55
Author(s):  
SURADWADEE THUNGMUNGMEE ◽  
NAKUNTWALAI WISIDSRI ◽  
WARACHATE KHOBJAI ◽  
THISAKORN DUMRONGPHUTTIDECHA ◽  
KHEMJIRA JAMKOM ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Sugar Content ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Objective: This study aimed to characterize physicochemical and chemical characteristics of Chaba maple (Hibiscus acetosella) homemade jam (CHJ)and determine its autoxidation ability.Methods: The physicochemical and chemical characteristics of CHJ were investigated. The color, viscosity, and pH were observed as physicochemicaldata while chemical properties were obtained from sugar content and total polyphenol content (TPC), determined using high-performance liquidchromatography refractometer and Folin–Ciocalteu assay, respectively. The antioxidant activities of CHJ were identified using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and nitric oxide (NO) radical scavenging ability methods.Results: The color and viscosity of CHJ were purple-red and 34,483.33±152.75 cP, respectively. The pH was at 3.78. The total sugar was not detectedin CHJ. The TPC of CHJ showed the highest (47.18±1.80 mg gallic acid equivalent [GAE]/g of jam) followed by Streamline (SL) (23.66±0.32 mgGAE/g of jam), Doikham (DK) (21.99±0.50 mg GAE/g of jam), and Best food (BF) (9.75±0.38 mg GAE/g of jam), respectively. Antioxidant activitiesof CHJ with %1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging of 100.00±1.39% and FRAP value of 1690.70±8.26 uM. Both of activitiesexhibited the highest activity and significantly different when compared with other jams. The %NO scavenging activity of CHJ and SL was 72.43±1.93%and 73.82±1.66%, respectively, which higher than DK and BF.Conclusion: This study shows good in both physicochemical and chemical characteristics of CHJ. The CHJ presents the highest TPC as well asantioxidant activities. Thus, a homemade jam of Chaba maple may be considered as a good source of antioxidants and functional foods.

scholarly journals Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux

2012 ◽  
Vol 48 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Ariana Costa-Mugica ◽  
Ana Elsa Batista- Gonzalez ◽  
Diadelis Mondejar ◽  
Yosdel Soto-López ◽  
Victor Brito-Navarro ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Ldl Oxidation ◽  
Dpph Radical ◽  
Polyphenol Content ◽  
Reducing Activity ◽  
Dpph Radical Scavenging ◽  
And Oxidative Stress

LDL oxidation and oxidative stress are closely related to atherosclerosis. Therefore, natural antioxidants have been studied as promising candidates. In the present study, the LDL oxidation inhibition activity of bioactive compounds from Halimeda incrassata seaweed. associated to antioxidant capacity, was evaluated in vitro. Experimental work was conducted with lyophilized aqueous extract and phenolic-rich fractions of the seaweed and their effect on LDL oxidation was evaluated using heparin-precipitated LDL (hep-LDL) with exposure to Cu2+ ions and AAPH as the free radical generator. H. incrassata had a protective effect for hep-LDL in both systems and the presence of phenolic compounds contributed to the activity where phenolic-rich fractions showed significant capacity for inhibition of oxidation mediated by Cu2+ ions. The observed effect could be related to the antioxidant potential of polar fractions evidenced by reducing activity and DPPH• radical scavenging. The results obtained in vitro further support the antioxidant and LDL oxidation inhibition properties of H. incrassata and further knowledge toward future phytotherapeutic application of the seaweed.

scholarly journals Usefulness of Urea as a Means of Improving the Solubility of Poorly Water-Soluble Ascorbyl Palmitate

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Yutaka Inoue ◽  
Daichi Niiyama ◽  
Isamu Murata ◽  
Ikuo Kanamoto
Keyword(s):  
Radical Scavenging ◽  
Ascorbyl Palmitate ◽  
Water Soluble ◽  
Mushroom Tyrosinase ◽  
Inhibition Assay ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Solubility Test ◽  
Crystal System ◽  
Dpph Radical Scavenging

The aim of this study was to evaluate complexes of L-ascorbyl palmitate (ASCP) and urea (UR). This evaluation involved differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), scanning electron microscopy (SEM), near-infrared spectroscopy (NIR), a solubility test, a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test, and a mushroom tyrosinase inhibition assay. Physicochemical evaluation revealed that ASCP/UR complexes form at a molar ratio of 1/12. The solubility test revealed that ASCP/UR complexes had increased solubility compared to ASCP. The DPPH radical scavenging test and mushroom tyrosinase inhibition assay revealed that the activity of ASCP/UR complexes was not impaired by complex formation. These results are probably due to the tetragonal crystal system of UR changing to a hexagonal crystal system and interaction with the alkyl group of ASCP.

scholarly journals A Study on the Biological Activity of Commonly Consumed Spring Herbs in Korea

2021 ◽  
Vol 19 (4) ◽  
pp. 665-678
Author(s):  
Hee-Jae Chung ◽  
Ae-Jung Kim
Keyword(s):  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Dpph Radical Scavenging Activity ◽  
Polyphenol Content ◽  
Third Place ◽  
Total Polyphenol ◽  
Dpph Radical Scavenging

Purpose: This study was conducted to study the superiority of Korean spring herbs by selecting eight spring herbs that are commonly consumed in Korea and evaluating their biological activity.Methods: Antioxidant (total polyphenol content, total flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and 2,2'-azino-bis-3-ethylbenzothiazoline–6-sulfonic acid (ABTS) radical scavenging activity) and enzyme activities (α-glucosidase inhibitory activity and angiotensin converting enzyme (ACE) inhibitory activity) were measured using the hot-water extract of the selected spring herbs.Results: In case of total polyphenol content, the shepherd’s purse, daylily, and gomchwi took first, second, and third place. In case of total flavonoid content, rape, shepherd’s purse and mugwort took first, second, and third place, and in case of DPPH radical scavenging activity, shepherd’s purse, daylily, and rape took first, second, and third place. In case of ABTS radical scavenging activity, the shepherd’s purse, mugwort and rape took first, second, and third place. In case of α-glucosidase inhibitory activity, mugwort, gomchwi, and wild garlic were classified in the first, second, and third place. Lastly, Korean angelica tree, rape, and wild garlic were classified in the first, second, and third, in case of ACE inhibitory activity.Conclusion: From these results, among the selected spring herbs, the shepherd’s purse and rape showed exceptional effects in antioxidant activity, mugwort in antidiabetic activity, and Korean angelica tree sprout in antihypertensive activity, respectively. Therefore, the present study is expected to provide basic data for future development of foods using spring herbs to remedy lifestyle-related diseases.

scholarly journals Chemical Properties and DPPH Radical Scavenging Ability of Sword Bean (Canavalia gladiata) Extract

2012 ◽  
Vol 44 (4) ◽  
pp. 441-446 ◽  
Author(s):  
Jong-Pil Kim ◽  
Yong-Shik Yang ◽  
Jin-Hee Kim ◽  
Hyang-Hee Lee ◽  
Eun-Sun Kim ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Chemical Properties ◽  
Dpph Radical ◽  
Canavalia Gladiata ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

scholarly journals Phenolic Composition and Antioxidant Activities of Opuntia ficus Indica L. Cladodes Related to Extraction Method

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Kechebar M. S. A. ◽  
Karoune S. ◽  
Laroussi K. ◽  
Djellouli A.
Keyword(s):  
Extraction Method ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Dpph Radical ◽  
Total Polyphenol ◽  
Opuntia Ficus Indica ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
Better Than

We studied the richness of phenolic compounds and the antioxidant activities of Opuntia ficus indica L. cladodes by comparing two types of extraction, maceration and soxhlet made with methanol / water (8 : 2, v/v). The results showed that the extraction by maceration is better than that by soxhlet with a total polyphenol concentration of 36.7 mg GAE/ g DW, a total antioxidant activity of 18.5 mg GAE/ g DW and an IC50 of 77.5 μg/ml against DPPH radical scavenging. These results are very vaforable for a sustainable valorisation of the prickly pear cladodes for medicinal, pharmacological and alimentary fields.

scholarly journals Enhancement of quercetin water solubility with steviol glucosides and the studies of biological properties

2015 ◽  
Vol 5 (12) ◽  
pp. 437 ◽  
Author(s):  
Thi Thanh Hanh Nguyen ◽  
Shin-Hye Yu ◽  
Jiyoun Kim ◽  
Eunbae An ◽  
Kyeonghwan Hwang ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Mushroom Tyrosinase ◽  
Human Beings ◽  
Inhibition Activity ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Human Intestinal Maltase ◽  
Dpph Radical Scavenging

Background: Quercetin, a flavonol contained in various vegetables and fruits, has various biological activities including anticancer, antiviral, anti-diabetic, and anti-oxidative. However, it has low oral bioavailability due to insolubility in water. Thus, the bioavailability of quercetin administered to human beings in a capsule form, was reported to be less than 1%, with only a small percentage of ingested quercetin getting absorbed in the blood. This leads to certain difficulties in creating highly effective medicinesMethods: Quercetin-rubusoside and quercetin-rebaudioside were prepared. The antioxidant activities of quercetin and Q-rubusoside were evaluated by DPPH radical scavenging method. Inhibition activities of quercetin and Quercetin-rubusoside were determined by measuring the remaining activity of 3CLpro with 200 μM inhibitor. The inhibition activity of quercetin, rubusoside and quercetin-rubusoside were determined by measuring the activity of human maltase which remains at 100 μM rubusoside or quercetin-rubusoside. The mushroom tyrosinase inhibition was assayed with the reaction mixture contained 3.3 mM L-DOPA in 50 mM potassium phosphate buffer (pH 6.8), and 10 U mushroom tyrosinase/ml with or without quercetin or quercetin-rubusoside. Results: With 10% rubusoside treatment, quercetin showed solubility of 7.7 mg/ml in water, and its solubility increased as the concentration of rubusoside increased; the quercetin solubility in water increased to 0.83 mg/mlas rubusoside concentration increased to 1 mg/ml. Quercetin solubilized in rubusoside solution showed DPPH radical-scavenging activity and mushroom tyrosinase inhibition activity, similar to that of quercetin solubilized in dimethyl-sulfoxide. Quercetin-rubusoside also showed 1.2 and 1.9 folds higher inhibition activity against 3CLpro of SARS and human intestinal maltase, respectively, than those of quercetin in DMSO.Conclusions: Quercetin can be solubilized in water with rebaudioside or rubusoside treatment. As Ru concentration increases, the solubility of quercetin in water increases. The solubilization of quercetin in Ru solution did not reduce its biological functions such as the DPPH radical-scavenging and mushroom tyrosinase activity; also, quercetin-rubusoside increased the inhibition activity against the 3CLpro of SARS and human intestinal maltase, when compared with the activity of quercetin in DMSO. Thus, rubusoside and rebaudioside are promising compounds which enhance the solubility of poorly water soluble compounds.Keywords: rubusoside, rebaudioside, flavonol, quercetin, human maltase, 3CLpro

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