Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

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THE CHARACTERISTICS AND ANTIOXIDANT ACTIVITIES OF CHABA MAPLE (HIBISCUS ACETOSELLA) HOMEMADE JAM

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s5.t0048 ◽

2019 ◽

pp. 52-55

Author(s):

SURADWADEE THUNGMUNGMEE ◽

NAKUNTWALAI WISIDSRI ◽

WARACHATE KHOBJAI ◽

THISAKORN DUMRONGPHUTTIDECHA ◽

KHEMJIRA JAMKOM ◽

...

Keyword(s):

High Performance ◽

Antioxidant Activities ◽

Sugar Content ◽

Radical Scavenging ◽

Chemical Properties ◽

Chemical Characteristics ◽

Dpph Radical ◽

Antioxidant Power ◽

Radical Scavenging Ability ◽

Dpph Radical Scavenging

Objective: This study aimed to characterize physicochemical and chemical characteristics of Chaba maple (Hibiscus acetosella) homemade jam (CHJ)and determine its autoxidation ability.Methods: The physicochemical and chemical characteristics of CHJ were investigated. The color, viscosity, and pH were observed as physicochemicaldata while chemical properties were obtained from sugar content and total polyphenol content (TPC), determined using high-performance liquidchromatography refractometer and Folin–Ciocalteu assay, respectively. The antioxidant activities of CHJ were identified using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and nitric oxide (NO) radical scavenging ability methods.Results: The color and viscosity of CHJ were purple-red and 34,483.33±152.75 cP, respectively. The pH was at 3.78. The total sugar was not detectedin CHJ. The TPC of CHJ showed the highest (47.18±1.80 mg gallic acid equivalent [GAE]/g of jam) followed by Streamline (SL) (23.66±0.32 mgGAE/g of jam), Doikham (DK) (21.99±0.50 mg GAE/g of jam), and Best food (BF) (9.75±0.38 mg GAE/g of jam), respectively. Antioxidant activitiesof CHJ with %1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging of 100.00±1.39% and FRAP value of 1690.70±8.26 uM. Both of activitiesexhibited the highest activity and significantly different when compared with other jams. The %NO scavenging activity of CHJ and SL was 72.43±1.93%and 73.82±1.66%, respectively, which higher than DK and BF.Conclusion: This study shows good in both physicochemical and chemical characteristics of CHJ. The CHJ presents the highest TPC as well asantioxidant activities. Thus, a homemade jam of Chaba maple may be considered as a good source of antioxidants and functional foods.

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Physicochemical and Antioxidant Properties of Iranian Commercial Honeys

Current Nutrition & Food Science ◽

10.2174/1573401314666180606090908 ◽

2019 ◽

Vol 15 (7) ◽

pp. 700-706

Author(s):

Mehdi Zarei ◽

Ali Fazlara ◽

Maedeh S. Hamedani

Keyword(s):

Reducing Sugars ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Dpph Radical Scavenging

Background: All over the world, the quality of commercial honeys varies greatly depending on many factors. Hence, this study was conducted to evaluate the physicochemical and antioxidant properties of Iranian commercial honeys to confirm their nutritional and economical quality. Methods: Fifty three honey samples from six different types, including alfalfa, milkvetch, lotus, thyme, coriander and multifloral honeys, were analyzed for pH, free acidity, electrical conductivity, Ash, moisture, hydroxymethylfurfural, reducing sugars, glucose, fructose, proline, color, total phenolic content, ferric reducing antioxidant power and DPPH radical-scavenging activity. Results: : In total, physicochemical properties of different honey types were in the range of 4.3 ± 0.49 to 5.2 ± 0.49 for pH, 13.81 ± 2.5 to 26.22 ± 2.8 for free acidity (meq/kg), 15.95 ± 0.45 to 17.98 ± 0.97 for moisture (%), 282.2 ± 50.3 to 578 ± 47 for electrical conductivity (µS/cm), 0.14 ± 0.04 to 0.38 ± 0.03 for ash (g/100 g), 68.66 ± 4.3 to 72.09 ± 6.4 for reducing sugars (g/100 g), 34.67 ± 2.6 to 36.7 ± 2.2 for glucose (g/100 g), 31.9 ± 4.75 to 37.04 ± 4.0 for fructose (g/100 g), 6.1 ± 5.8 to 22.85 ± 10.9 for hydroxymethylfurfural (mg/kg), 426.7 ± 53.91 to 593.9 ± 108.6 for proline (mg/kg), 46.27 ± 4.3 to 96.04 ± 14.4 for color (mm) and 245.4 ± 48.45 to 573.9 ± 69.3 for total phenolic (mg/kg) content. In terms of antioxidant capacity, lotus and thyme honeys were preferable, while the highest level of DPPH radical scavenging activity and ferric reducing antioxidant power were observed in lotus and thyme honeys. Conclusion: These results confirmed that the Iranian commercial honeys meet the quality standards established by national and international regulatory agencies.

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Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill bark

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2017-0063 ◽

2018 ◽

Vol 29 (2) ◽

pp. 217-224 ◽

Cited By ~ 8

Author(s):

Oluwafemi Adeleke Ojo ◽

Adebola Busola Ojo ◽

Basiru Olaitan Ajiboye ◽

Babatunji Emmanuel Oyinloye ◽

Ayodele Jacob Akinyemi ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Stem Bark ◽

Dependent Manner ◽

Dpph Radical ◽

Irvingia Gabonensis ◽

Radical Scavenging Ability ◽

Dpph Radical Scavenging ◽

Phenolic Extracts

AbstractBackground:Irvingia gabonensisstem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis. This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts fromI. gabonensis(Aubry-Lecomte ex O‘Rorke) Baill bark.Methods:Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability. Inhibitory activities on AChE and BChE were evaluated.Results:The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin). The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+to Fe2+) (5.94 mg ascorbic acid equivalent/100 g). Extracts ofI. gabonensisinhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.Conclusions:Hence, possible mechanism through which the stem bark executes their anti-Alzheimer’s disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.

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Antioxidant Peptides from Collagen Hydrolysate of Redlip Croaker (Pseudosciaena polyactis) Scales: Preparation, Characterization, and Cytoprotective Effects on H2O2-Damaged HepG2 Cells

Marine Drugs ◽

10.3390/md18030156 ◽

2020 ◽

Vol 18 (3) ◽

pp. 156 ◽

Cited By ~ 3

Author(s):

Wan-Yi Wang ◽

Yu-Qin Zhao ◽

Guo-Xu Zhao ◽

Chang-Feng Chi ◽

Bin Wang

Keyword(s):

Hepg2 Cells ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Pharmaceutical Products ◽

Amino Acid Sequences ◽

Degree Of Hydrolysis ◽

Dpph Radical ◽

Antioxidant Peptides ◽

Collagen Peptides

Bioactive peptides from fish collagens with antioxidant properties have become a topic of great interest for health, food, and processing/preservation industries. To explore the high-value utilized way of scales produced during the fish processing, collagen hydrolysates of redlip croaker (Pseudosciaena polyactis) scales were prepared using six different proteases, and the hydrolysate (RSCH) prepared using neutrase showed the highest degree of hydrolysis (21.36 ± 1.18%) and 2,2-diphenyl-1-picrylhydrazyl (DPPH·) radical scavenging activity (30.97 ± 1.56%) among the six hydrolysates. Subsequently, six antioxidant peptides were purified from RSCH using membrane ultrafiltration and serial chromatography, and their amino acid sequences were identified as DGPEGR, GPEGPMGLE, EGPFGPEG, YGPDGPTG, GFIGPTE, and IGPLGA with molecular masses of 629.61, 885.95, 788.96, 762.75, 733.80, and 526.61 Da, respectively. Among six collagen peptides, GPEGPMGLE, EGPFGPEG, and GFIGPTE exhibited the strongest scavenging activities on DPPH· radical (EC50 0.59, 0.37, and 0.45 mg/mL), hydroxyl radical (EC50 0.45, 0.33, and 0.32 mg/mL), and superoxide anion radical (EC50 0.62, 0.47, and 0.74 mg/mL). GPEGPMGLE, EGPFGPEG, and GFIGPTE showed high inhibiting ability on lipid peroxidation in a linoleic acid model system and protective activities on oxidation-damaged DNA. More importantly, GPEGPMGLE, EGPFGPEG, and GFIGPTE could protect HepG2 cells from H2O2-induced oxidative damage through decreasing the levels of reactive oxygen species (ROS) and MDA and activating intracellular antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). These results suggested that six collagen peptides (RCP1–RCP6), especially GPEGPMGLE, EGPFGPEG, and GFIGPTE, might serve as potential antioxidants applied in nutraceutical and pharmaceutical products.

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Phytochemical screening and antioxidant activity of Melastoma malabathricum and Chromolaena odorata by DPPH radical scavenging method

Food Research ◽

10.26656/fr.2017.5(s4).006 ◽

2021 ◽

Vol 5 (S4) ◽

pp. 30-37

Author(s):

N.H. Ismail ◽

Amira N.H. ◽

S.N.H.M. Latip ◽

W.Z.W.M. Zain ◽

S.N.A. Aani ◽

...

Keyword(s):

Antioxidant Activity ◽

Nutritive Value ◽

Chemical Constituents ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanol Extract ◽

Dpph Radical ◽

Chromolaena Odorata ◽

Melastoma Malabathricum ◽

Dpph Radical Scavenging

Melastoma malabathricum and Chromolaena odorata are classified under broad-leaved weeds that are widely spread in the open land area. Melastoma malabathricum is commonly known as ‘‘senduduk’’, and C. odorata is locally known as “Pokok Kapal Terbang”. Both weeds are categorized as potential weeds as they have high nutritive value and are rich in chemical compounds. This study aimed to determine their chemical constituents and possible potential for antioxidant activity as these weeds have been reported to possess antioxidant properties. Screening of the plants was performed using standard methods and revealed the existence of various secondary metabolites such as saponins, terpenoids, phenols, tannins, and flavonoids of both weed extracts. Antioxidant activity was validated by the DPPH radical scavenging assay of M. malabathricum and C. odorata crude ethanol extract. The IC50 values for the percentage radical scavenging effects for the extracts were determined. The IC50 value of M. malabatrichum extract was 81.116 μg/mL, C. odorata was 312.903 μg/mL, Vitamin C was 31.023 μg/mL and BHA was 71.521 μg/mL respectively. The study showed that the antioxidant activity of M. malabatrichum was more potent and better than C. odorata.

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Antioxidant Peptides from the Protein Hydrolysate of Spanish Mackerel (Scomberomorous niphonius) Muscle by in Vitro Gastrointestinal Digestion and Their in Vitro Activities

Marine Drugs ◽

10.3390/md17090531 ◽

2019 ◽

Vol 17 (9) ◽

pp. 531 ◽

Cited By ~ 7

Author(s):

Zhao ◽

Yang ◽

Wang ◽

Zhao ◽

Chi ◽

...

Keyword(s):

Protein Hydrolysate ◽

Radical Scavenging ◽

Model Systems ◽

Dpph Radical ◽

Antioxidant Peptides ◽

Gastrointestinal Digestion ◽

Spanish Mackerel ◽

In Vitro Gastrointestinal Digestion ◽

Dpph Radical Scavenging

For the full use of Spanish mackerel (Scomberomorous niphonius) muscle to produce antioxidant peptides, the proteins of Spanish mackerel muscle were separately hydrolyzed under five kinds of enzymes and in vitro gastrointestinal digestion, and antioxidant peptides were isolated from the protein hydrolysate using ultrafiltration and multiple chromatography methods. The results showed that the hydrolysate (SMPH) prepared using in vitro GI digestion showed the highest degree of hydrolysis (27.45 ± 1.76%) and DPPH radical scavenging activity (52.58 ± 2.68%) at the concentration of 10 mg protein/mL among the six protein hydrolysates, and 12 peptides (SMP-1 to SMP-12) were prepared from SMPH. Among them, SMP-3, SMP-7, SMP-10, and SMP-11 showed the higher DPPH radical scavenging activities and were identified as Pro-Glu-Leu-Asp-Trp (PELDW), Trp-Pro-Asp-His-Trp (WPDHW), and Phe-Gly-Tyr-Asp-Trp-Trp (FGYDWW), and Tyr-Leu-His-Phe-Trp (YLHFW), respectively. PELDW, WPDHW, FGYDWW, and YLHFW showed high scavenging activities on DPPH radical (EC50 1.53, 0.70, 0.53, and 0.97 mg/mL, respectively), hydroxyl radical (EC50 1.12, 0.38, 0.26, and 0.67 mg/mL, respectively), and superoxide anion radical (EC50 0.85, 0.49, 0.34, and 1.37 mg/mL, respectively). Moreover, PELDW, WPDHW, FGYDWW, and YLHFW could dose-dependently inhibit lipid peroxidation in the linoleic acid model system and protect plasmid DNA (pBR322DNA) against oxidative damage induced by H2O2 in the tested model systems. In addition, PELDW, WPDHW, FGYDWW, and YLHFW could retain their high activities when they were treated under a low temperature (<60 °C) and a moderate pH environment (pH 5–9). These present results indicate that the protein hydrolysate, fractions, and isolated peptides from Spanish mackerel muscle have strong antioxidant activity and might have the potential to be used in health food products.

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Comparative Chemical Composition and Antioxidant Properties of the Essential Oils of three Sideritis libanotica Subspecies

Natural Product Communications ◽

10.1177/1934578x1501000670 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000 ◽

Cited By ~ 2

Author(s):

Carmen Formisano ◽

Filomena Oliviero ◽

Daniela Rigano ◽

Nelly Apostolides Arnold ◽

Felice Senatore

Keyword(s):

Essential Oils ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Moderate Activity ◽

Ferric Ion ◽

Main Constituent ◽

Dpph Radical ◽

Hexadecanoic Acid ◽

Phytochemical Composition ◽

Dpph Radical Scavenging

The phytochemical composition of the essential oils of three Sideritis libanotica subspecies, namely S. libanotica ssp. libanotica, S. libanotica ssp. linearis and S. libanotica ssp. michroclamys, all collected in Lebanon, was analyzed by GC and GC-M S. The diterpene sideridiol was recognized as the main constituent of both S. libanotica ssp. libanotica (50.8%) and S. libanotica ssp. michroclamys (18.4%) oils, while hexadecanoic acid (10.5%) prevailed in S. libanotica ssp. linearis. The antioxidant activity of the oils was studied in two cell free systems by DPPḢ radical scavenging and ferric ion reduction (FRAP) assays; only S. libanotica ssp. linearis showed a moderate activity when assayed by the FRAP test (0.6± 0.01 mmol TE/mL).

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Radical Scavenging and Antioxidant Activities of Essential Oil Components – An Experimental and Computational Investigation

Natural Product Communications ◽

10.1177/1934578x1501000135 ◽

2015 ◽

Vol 10 (1) ◽

pp. 1934578X1501000 ◽

Cited By ~ 7

Author(s):

Farukh S. Sharopov ◽

Michael Wink ◽

William N. Setzer

Keyword(s):

Essential Oil ◽

Electronic Properties ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Dpph Radical ◽

Frap Assay ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power ◽

Oil Components ◽

Dpph Radical Scavenging

The antioxidant activities of eighteen different essential oil components have been determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, the 2,2 ’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation assay, and the ferric reducing antioxidant power (FRAP) assay. The phenolic compounds, carvacrol, thymol, and eugenol, showed the best antioxidant activities, while camphor, menthol, and menthone were the least active. The structural and electronic properties of the essential oil components were assessed using density functional theory (DFT) at the B3LYP/6-311++G** level. Correlations between calculated electronic properties and antioxidant activities were generally poor, but bond-dissociation energies (BDEs) seem to correlate with DPPH radical-scavenging activities, and the ferric reducing antioxidant power (FRAP) assay correlated with vertical ionization potentials calculated at the Hartree-Fock/6-311++G** level.

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ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.10 ◽

2018 ◽

Vol 10 (1) ◽

pp. 44 ◽

Cited By ~ 1

Author(s):

Riza Shabrina ◽

Berna Elya ◽

Arikadia Noviani

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Group Identification ◽

Radical Scavenging ◽

Dpph Radical ◽

Frap Assay ◽

Antioxidant Power ◽

Dpph Radical Scavenging

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

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In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods

BioMed Research International ◽

10.1155/2018/1824790 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Gauhar Rehman ◽

Muhammad Hamayun ◽

Amjad Iqbal ◽

Saif Ul Islam ◽

Saba Arshad ◽

...

Keyword(s):

Glucose Uptake ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Yeast Cells ◽

In Vitro Assays ◽

Dpph Radical ◽

Dpph Radical Scavenging

The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, the DPPH radical scavenging capacity of the extract was increased to a maximum value of 43.3% at 80 μg/ml, which was then decreased to 41.9% at 100 μg/ml. From the results, it was concluded that EECNP possess good antidiabetic and antioxidant properties as shown by in vitro assays.

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