scholarly journals Phenolic Composition and Antioxidant Activities of Opuntia ficus Indica L. Cladodes Related to Extraction Method

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Kechebar M. S. A. ◽  
Karoune S. ◽  
Laroussi K. ◽  
Djellouli A.
Keyword(s):  
Extraction Method ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Dpph Radical ◽  
Total Polyphenol ◽  
Opuntia Ficus Indica ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
Better Than

We studied the richness of phenolic compounds and the antioxidant activities of Opuntia ficus indica L. cladodes by comparing two types of extraction, maceration and soxhlet made with methanol / water (8 : 2, v/v). The results showed that the extraction by maceration is better than that by soxhlet with a total polyphenol concentration of 36.7 mg GAE/ g DW, a total antioxidant activity of 18.5 mg GAE/ g DW and an IC50 of 77.5 μg/ml against DPPH radical scavenging. These results are very vaforable for a sustainable valorisation of the prickly pear cladodes for medicinal, pharmacological and alimentary fields.

scholarly journals ANTIOXIDANT ACTIVITIES OF SOME LESS UTILIZED EDIBLE FRUITS

2017 ◽  
pp. 28-31
Author(s):  
Sumita Dasgupta ◽  
Maitry Pandya ◽  
Nirali Patel
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phyllanthus Emblica ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Frap Assay ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging

Objective: The objective of the present study was to evaluate the total phenolic (TPC) and flavonoid content (TFC) of five less utilized fruits such as Aegle marmelos, Spondias pinnata, Limonia acidissima, Averhoa carambola, Crescentia cujete and was compared with Phyllanthus emblica, (Amla) well known for its antioxidant activities. Total phenolic and flavonoid contents of samples were correlated with antioxidant activities like 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) free radical scavenging assay, Ferric reducing antioxidant potential (FRAP) assay and total antioxidant capacity (TAC).Methods: The total phenolic of each fruit extract were determined by the Folin-Ciocalteu method with some modifications and the total flavonoids were estimated by Aluminum trichloride colourimetric method. The DPPH antioxidant assay, The FRAP assay and TAC were determined spectrophotometrically.Results: The total phenolics were expressed as mg/100g Gallic acid equivalent (mg GAE/100 gm) and the total flavonoids were expressed as mg/100g Quercetin equivalent (mg QE/100 gm). TPC was found to be maximum in Spondias pinnata with 142.16 mg GAE/100 gm where as TFC was maximum in Phyllanthus emblica with 91.1 mgQE/100 gm. DPPH radical scavenging activity was expressed n percentage(%), FRAP values expressed as mg/100g Ascorbic equivalent (AAE) and the total antioxidant activity was expressed as mg/100g Ascorbic equivalent. Maximum DPPH radical scavenging activity was shown by Spondias pinnata (93.75%), FRAP values were maximum in Phyllanthus emblica with 72.6 mg AAE/100 gm and total antioxidant capacity was found to be highest in Spondias pinnata (50.1 mg AAE/100 gm).Conclusion: Spondias pinnata, an underutilized fruit, was found to be promising with antioxidant activities comparable to Phyllanthus emblica.

Pill-millipedes (Arthrosphaera: Sphaerotheriida) of the Western Ghats: a future source of antioxidants?

2015 ◽  
Vol 35 (03) ◽  
pp. 117-124 ◽  
Author(s):  
Cheviri Nair Ambarish ◽  
Kandikere Ramaiah Sridhar
Keyword(s):  
Antioxidant Activity ◽  
Western Ghats ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Ferrous Ion ◽  
Total Antioxidant Activity ◽  
Dpph Radical ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
The Western Ghats

Along with plant sources, arthropods (such as millipedes) are also known for their antioxidant activities that can help combat human ailments. Therefore, methanolic extracts of males and females of two endemic giant pill-millipedes of the Western Ghats (Arthrosphaera fumosaPocock andArthrosphaera magnaAttems) were evaluated for antioxidant potential. The bioactive principles and activities assessed were total phenolics, total antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl or DPPH radical scavenging, ferrous ion chelation, reducing power and hydrogen peroxidase scavenging. Significant differences were shown between the two species, the sexes and the sexes within millipede species in total antioxidant activity, DPPH radical scavenging and ferrous ion chelation. Total phenolics and EC50of DPPH radical scavenging activity differed significantly only between species and the sexes. This study clearly demonstrated that the antioxidant capacity of the methanolic extract ofArthrosphaeraspp. is comparable to the ascorbic acid standard and possesses potential scavenging ability against reactive oxygen species generated during oxidative stress.

scholarly journals Analysis of Total Antioxidant Capacities of Various Seaweeds Grown off the Korean Coast (P06-110-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Chai-Hyeon Lee ◽  
Ye-Na Park ◽  
Hyeung-Rak Kim ◽  
Sang Gil Lee ◽  
Bohkyung Kim
Keyword(s):  
Functional Food ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Antioxidant Capacities ◽  
Scavenging Capacity ◽  
Total Antioxidant ◽  
Radical Generation ◽  
Dpph Radical Scavenging

Abstract Objectives It has been a long time since seaweeds have been used for food ingredients in Asian countries. Recently, a body of research has revealed the health benefits of bioactive compounds in seaweeds, especially their antioxidant capacities. Although numerous seaweeds inhabit in the ocean, only a small percentage has been explored for functional food. Therefore, the purpose of this study was to analyze the antioxidant capacities of various seaweeds grown off the Korean coast to screen for the potential functional food sources. Methods Ten ethanol extracts of Korean seaweeds were provided by the National Marine Biodiversity Institute of Korea, which included Scytosiphon gracilis, Scytosiphon lomentaria, Sargassum muticum, Sargassum confusum, Petrospongium rugosum, Sargassum fusiforme, Petalonia fascia, Sargassum nigrifolium, Ishige foliacea, and Myelophycus simplex. Hundred mg/L of samples in 80% methanol was used to measure their ABTS, DPPH, and superoxide radical scavenging activities. Mg vitamin C equivalent antioxidant capacity (VCEAC)/100 mg was used to express the ABTS and DPPH radical scavenging capacities. For the superoxide radical scavenging capacity, inhibition rate of superoxide radical generation (%) was calculated. Results Among the ten seaweeds, S. nigrifolium and I. foliacea exhibited the most significant radical scavenging capacities. DPPH radical scavenging capacities of I. foliacea and S. nigrifolium were 122.4 mg VCEAC/100 mg and 95.8 mg VCEAC/100 mg, respectively. For ABTS radical scavenging capacity, I. foliacea exhibited 178.5 mg VCEAC/100 mg, followed by S. nigrifolium as 80.9 mg VCEAC/100 mg. I. foliacea inhibited about 68% of superoxide radical generation followed by S. nigrifolium (40.6%) while vitamin C as positive control inhibited about 28.9% of superoxide radical generation. The antioxidant capacities measured by the three assays were positively correlated with each other. Conclusions The current study explored total antioxidant capacities of various Korean seaweeds and found I. foliacea and S. nigrifolium as the most potential antioxidant-rich food resources. Further research would be warranted to investigate bioactive compounds from S. nigrifolium and I. foliacea. Funding Sources This work was supported by the Pukyong National University Research Fund in 2018.

scholarly journals Different Extraction Solvents and Sample Preparation Methods Affect the Antioxidant Activities and Total Phenol Contents of Theobroma Cacao Powder (P01-016-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Hyoeun Yoo ◽  
Hyun-Sook Kim
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Methanol Solution ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Freeze Dried ◽  
Dpph Radical Scavenging

Abstract Objectives Cacao(Theobroma cacao) has been widely consumed since more than 4000 years ago which was regarded as sacred food. Cacao has been considered as a strong antioxidant due to its abundant phenolic and especially flavonoid contents. In this experiment, different extraction solvents and methods were used to analyze whether these factors affect the antioxidant activities and phytochemical compounds of cacao powder extracts. Methods Four different cacao extract samples were examined to identify their anti-oxidative activities; raw cacao powder extracted in 70% methanol solution(RM), raw cacao powder extracted in 80% ethanol solution(RE), freeze dried cacao powder extracted in 70% methanol solution(FM), freeze dried cacao powder extracted in 80% ethanol solution(FE). Total phenolic compound content was calculated based on gallic acid standard curve and total flavonoid content was calculated based on the standard curve of quercetin. ABTS scavenging activity was experimented with ABTS reagent diluted by PBS(pH7.4) to make the O.D. value 0.7 ± 0.02 at 734 nm before using 2.5 mM ABTS. The DPPH radical scavenging activity was assessed by 1 mM DPPH methanol solution. Results The contents of total phenol compounds were 45.4 mg GAE/g(RM), 37.3 mg GAE/g(RE), 50.6 mg GAE/g(FM), and 52.7 mg GAE/g(FE). The contents of total flavonoid compounds were 34.3 mg QE/g(RM), 29.7 mg QE/g(RE), 85.1 mg QE/g(FM), and 89.7 mg QE/g(FE). FM group showed the highest ABTS radical scavenging activity which is a marker of antioxidant activity but the other sample groups represented almost as high ABTS radical scavenging activity as FM group. RM group showed the highest DPPH radical scavenging activity which is also a marker of antioxidant activity. Conclusions The results show that different extract solvents and sample preparation methods affect DPPH radical scavenging activity, total phenol and flavonoid contents of cacao powder extracts. Therefore, appropriate methods should be applied according to the purpose of the study. Funding Sources Department of Food and Nutrition, Sookmyung Women's University, Republic of Korea.

scholarly journals THE CHARACTERISTICS AND ANTIOXIDANT ACTIVITIES OF CHABA MAPLE (HIBISCUS ACETOSELLA) HOMEMADE JAM

2019 ◽  
pp. 52-55
Author(s):  
SURADWADEE THUNGMUNGMEE ◽  
NAKUNTWALAI WISIDSRI ◽  
WARACHATE KHOBJAI ◽  
THISAKORN DUMRONGPHUTTIDECHA ◽  
KHEMJIRA JAMKOM ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Sugar Content ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Objective: This study aimed to characterize physicochemical and chemical characteristics of Chaba maple (Hibiscus acetosella) homemade jam (CHJ)and determine its autoxidation ability.Methods: The physicochemical and chemical characteristics of CHJ were investigated. The color, viscosity, and pH were observed as physicochemicaldata while chemical properties were obtained from sugar content and total polyphenol content (TPC), determined using high-performance liquidchromatography refractometer and Folin–Ciocalteu assay, respectively. The antioxidant activities of CHJ were identified using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and nitric oxide (NO) radical scavenging ability methods.Results: The color and viscosity of CHJ were purple-red and 34,483.33±152.75 cP, respectively. The pH was at 3.78. The total sugar was not detectedin CHJ. The TPC of CHJ showed the highest (47.18±1.80 mg gallic acid equivalent [GAE]/g of jam) followed by Streamline (SL) (23.66±0.32 mgGAE/g of jam), Doikham (DK) (21.99±0.50 mg GAE/g of jam), and Best food (BF) (9.75±0.38 mg GAE/g of jam), respectively. Antioxidant activitiesof CHJ with %1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging of 100.00±1.39% and FRAP value of 1690.70±8.26 uM. Both of activitiesexhibited the highest activity and significantly different when compared with other jams. The %NO scavenging activity of CHJ and SL was 72.43±1.93%and 73.82±1.66%, respectively, which higher than DK and BF.Conclusion: This study shows good in both physicochemical and chemical characteristics of CHJ. The CHJ presents the highest TPC as well asantioxidant activities. Thus, a homemade jam of Chaba maple may be considered as a good source of antioxidants and functional foods.

scholarly journals Radical Scavenging and Antioxidant Activities of Essential Oil Components – An Experimental and Computational Investigation

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Farukh S. Sharopov ◽  
Michael Wink ◽  
William N. Setzer
Keyword(s):  
Essential Oil ◽  
Electronic Properties ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Oil Components ◽  
Dpph Radical Scavenging

The antioxidant activities of eighteen different essential oil components have been determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, the 2,2 ’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation assay, and the ferric reducing antioxidant power (FRAP) assay. The phenolic compounds, carvacrol, thymol, and eugenol, showed the best antioxidant activities, while camphor, menthol, and menthone were the least active. The structural and electronic properties of the essential oil components were assessed using density functional theory (DFT) at the B3LYP/6-311++G** level. Correlations between calculated electronic properties and antioxidant activities were generally poor, but bond-dissociation energies (BDEs) seem to correlate with DPPH radical-scavenging activities, and the ferric reducing antioxidant power (FRAP) assay correlated with vertical ionization potentials calculated at the Hartree-Fock/6-311++G** level.

scholarly journals ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 44 ◽  
Author(s):  
Riza Shabrina ◽  
Berna Elya ◽  
Arikadia Noviani
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Group Identification ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Dpph Radical Scavenging

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

STUDY OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF SAXICOLOUS LICHEN $ \textit{XANTHOPARMELIA STENOPHYLLA} $

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 132-137
Author(s):  
A.G. Simonyan ◽  
R.R. Sargsyan ◽  
H.H. Panosyan ◽  
A.H. Trchounian
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Gram Positive Bacteria ◽  
Acetone Extracts ◽  
Dpph Radical Scavenging

Antibacterial and antioxidant activities of different extracts of saxicolous lichen Xanthoparmelia stenophylla sampled from Norashen, Gegharkunik Province of Armenia were studied. Methanol, ethanol and acetone extracts of lichen thalli were demonstrated to have activity against only tested gram-positive bacteria. Methanol extract of the lichen showed the highest amount of DPPH radical scavenging activity (~68%). Our studies did not reveal any significant antibacterial and antioxidant activities of aqueous extract.

scholarly journals In VitroAntioxidant Properties of Three Varieties ofAllium sativumL. Extracts

2010 ◽  
Vol 7 (s1) ◽  
pp. S573-S579 ◽  
Author(s):  
R. T. Narendhirakannan ◽  
K. Rajeswari
Keyword(s):  
Free Radical ◽  
Allium Sativum ◽  
Preventive Intervention ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Garlic Bulb ◽  
Total Antioxidant ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities.Allium sativumL. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assaysi.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability,Allium sativumL. could be used as a potential preventive intervention for free radical-mediated diseases.

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

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