scholarly journals Association of general blood count indicators with the severity of COVID-19 in hospitalized patients

2021 ◽  
Vol 2 (1) ◽  
pp. 90-101
Author(s):  
N. S. Gubenko ◽  
A. A. Budko ◽  
A. G. Plisyuk ◽  
I. A. Orlova
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Clinical Condition ◽  
Blood Test ◽  
Medical Center ◽  
Absolute Number ◽  
Hospitalized Patients ◽  
Lung Damage ◽  
The Absolute ◽  
Severity Of The Disease

Purpose: to study the relationship of the indicators of the general blood test with the severity of the course of COVID-19 in hospitalized patients.Materials and methods: the study included 165 patients (92 men — 55.8%, and 73 women — 44.2%, the average age — 59.9 years) who were treated at the Moscow State University Medical Center in the period from April to June 2020 with a diagnosis of COVID-19. All patients underwent: general blood test, CRP, CT of the lungs. The severity of the clinical condition was assessed using the SHOCK-COVID and NEWS-2 scales.Results: a more severe clinical condition of patients and a greater severity of lung damage on admission were statistically significantly associated with a decrease in the number of red blood cells and hemoglobin, as well as with a greater width of the distribution of red blood cells (RDW-SD). The rate of erythrocyte sedimentation (ESR) was significantly associated with the clinical condition of patients evaluated by SHOCK-COVID (r=0.61, p<0.001) and the marker of CRP inflammation (r=0.55, p<0.001). An increase in the absolute number of neutrophils (N), a decrease in the absolute number of lymphocytes (L), and, as a result, an increase in the N/L ratio index was a marker of a more severe course of the disease. It was the N/L index that had the maximum correlation coefficient with the most commonly used marker of systemic inflammation - CRP (r=0.50, p<0.001). The decrease in the level of CRP by discharge was associated with a significant decrease in ESR (r=0.36, p<0.001), the index of the ratio of neutrophil and lymphocyte levels (N/L) (r=0.39, p<0.001), and an increase in the width of the distribution of red blood cells (RDW-SD r=0.25, p<0.01; RDW-CV r=0.57, p<0.001).Conclusions: the most informative indicators of the general blood test at admission to the hospital, allowing to assess the severity of the disease — the width of the distribution of red blood cells, the index of the ratio of neutrophils to lymphocytes and ESR.

scholarly journals The eff ect of poliodoxin and tilmox 25% on the morphological and biochemical parameters of the blood of broiler chickens

2020 ◽  
pp. 72-79
Author(s):  
A. Tyshkivska ◽  
V. Dukhnitsky ◽  
M. Tyshkivsky
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Clinical Condition ◽  
Biochemical Parameters ◽  
Blood Test ◽  
Broiler Chickens ◽  
White Blood Cells ◽  
Research Groups ◽  
Hemoglobin Content ◽  
Behavioral Reactions

The article presents the results of studies of the eff ect of the drug “Poledoxin”, which belongs to the group of tetracyclines and the drug “Tilmox 25%”, belonging to the group of macrolides, on some morphological and biochemical parameters of blood of Coobb-500 broiler chickens. It has been established that the use of the preparations “Poledoxin” and “Tilmox 25 %” for healthy broiler chickens according to the scheme recommended for treating birds with ornithobacteriosis and infections complicated by the causative agent of ornithobacteriosis does not cause changes in the clinical condition and behavioral reactions of the bird. A blood test found that under the infl uence of the drug “Poledoxin”, the number of red blood cells in the blood of broiler chickens increased by 4–59 % (p< 0,01) in chickens which used the drug “Tilmox 25 %” – by 5–46 % relative to control. The hematocrit value was less than the control indicator by 8 % in chickens that were treated with Poledoxin after 6 days of experiment (p< 0,05) in chickens that were used with tilmox 25 % – by 10 % (p< 0,05) through 6 and 9 days of experience. The hemoglobin content in the blood of poultry of the studied groups one day after drinking antibiotic solutions was less than the control by 7 and 8 %, respectively (p< 0,05, p< 0,01), and after 4 days – by 11 and 10 % (p< 0,01). Under the infl uence of Pledoxin, the volume of 1 erythrocyte (MCV, μm 3) in the blood of chickens was less than the control by 23–39 % (p< 0,05, p< 0,01), and when exposed to tilmox 25% – by 16–35 % (p< 0,01). The hemoglobin content in 1 erythrocyte (MCH, pg) was 62–83 % of the control index when exposed to Poledoxin (p< 0,01) and 69–86 % when exposed to tylmox 25 % (p< 0,01). The number of leukocytes in the blood of a bird under the infl uence of “Poledoxin” decreased by 8 % after a day (p< 0,01), and increased by 6 and 7 % after 3 and 4 days (p< 0,01). A decrease in the number of leukocytes in the blood of chickens of both research groups by 5–11 % (p< 0,001, p< 0,01) was established after 8 and 9 days. Key words: Poledoxin, Tilmox 25 %, broiler chickens, red blood cells, hematocrit, hemoglobin, white blood cells.

‘Nucleated red blood cells’ as hypoxia marker in clinical analysis of blood in patients with coronavirus infection

2021 ◽  
pp. 23-24
Author(s):  
O. Yu. Dorn ◽  
F. M. Orifova ◽  
E. G. Stepanova
Keyword(s):  
Clinical Practice ◽  
Blood Cells ◽  
Blood Test ◽  
Clinical Analysis ◽  
Lung Damage ◽  
Hypoxic Stress ◽  
Urgent Problem ◽  
Nucleated Red Blood Cells ◽  
Hypoxia Marker ◽  
Coronavirus Infection

Assessment of the condition of a patient with coronavirus infection is an urgent problem in clinical practice. The parameter ’nucleated erythrocytes’ in a clinical blood test can be used in clinical practice as a marker of hypoxia in case of lung damage, since it objectively reflects the consequences of acute hypoxic stress in the patient’s body.

To hematology of scurvy

1904 ◽  
Vol 4 (1-2) ◽  
pp. 104-105
Author(s):  
M Beloglazov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Gradual Increase ◽  
Absolute Amount ◽  
The Absolute

11 people of scurvy patients of various ages (from 14 to 52 years old) were investigated by the author from the side of blood changes. The main results of the study are as follows: i) The absolute amount of hemoglobin in all the cases studied is reduced; the gradual increase during recovery does not reach the norm even when the number of red blood cells becomes normal.

scholarly journals Influence of seasonality and pollution on the hematological parameters of the estuarine fish Centropomus parallelus

2013 ◽  
Vol 61 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Robson Seriani ◽  
Denis Moledo de Souza Abessa ◽  
Camilo D. S. Pereira ◽  
Aline A. Kirschbaum ◽  
Andrea Assunção ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Absolute Number ◽  
Aquatic Pollution ◽  
Estuarine Fish ◽  
Corpuscular Volume ◽  
Tropical Estuarine ◽  
Non Destructive

This study investigated the hematological parameters of the tropical estuarine fish Centropomus parallelus and their use as a non-destructive biomarker for aquatic pollution. Individuals were collected, in summer and winter, at two estuaries, Cananéia (CAN) and São Vicente (SVE), and blood was extracted by caudal puncture. The evaluated parameters were hematocrit (Ht), red blood cells (RBC), Mean Corpuscular Volume (MCV), and the leukocyte (WBC) and thrombocyte counts. Fishes from CAN showed higher values of absolute number of thrombocytes in the summer. The fishes from SVE presented lower values of Ht and MCV in winter. Comparing the hematological parameters of fishes from these two sites, Ht, MCV, WBC and RBC were higher in fishes from SVE in the summer, whereas during the winter, Ht and thrombocytes were higher in animals from SVE. The results allow attributing the changes in the blood of fishes to seasonality and the presence of contaminants.

scholarly journals ENDOTHELIAL FUNCTION AND MORPHOFUNCTIONAL STATE OF BLOOD CELLS IN THE BASINS OF THE ISCHEMIC LIMBS IN THE DIFFERENT TYPES OF SURGICAL TREATMENT OF ATHEROSCLEROSIS OBLITERANS

2011 ◽  
Vol 2 (1) ◽  
pp. 41-46
Author(s):  
I I Katelnitsky ◽  
S A Pleskachev ◽  
M A Burikov ◽  
A S Matsionis ◽  
P E Povilaytite
Keyword(s):  
Surgical Treatment ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Lower Limbs ◽  
Lumbar Sympathectomy ◽  
Treatment Implementation ◽  
Different Types ◽  
Red Blood Cell Membranes ◽  
Severity Of The Disease ◽  
Atherosclerosis Obliterans

The aim of the investigation was to study the morphological condition of blood cells in the basins of the ischemic limbs and their dynamics as a result of various types of surgical treatment. Implementation of combined surgical treatment has a more normalizing effect in comparison with isolated reconstructive surgical treatment. The use of lumbar sympathectomy in patients with occlusive lesions of arteries of lower limbs and varying degrees of ischemia reduces endothelial dysfunction and normalizes a number of parameters describing the morphology and functioning of red blood cells and platelets. There was detected the deformation of red blood cells indicating that the restoration of the plasticity of red blood cell membranes significally reduced the degree of agglutination of red blood cells. According to obtained data the influence of sympathectomy depends on the severity of the disease in general the highest efficiency is observed at the II and III degree of ischemia.

Drug‑induced hemolytic anemia

2021 ◽  
pp. 49-56
Author(s):  
O. D. Ostroumova ◽  
S. A. Bliznyuk ◽  
A. I. Kochetkov ◽  
A. G. Komarova
Keyword(s):  
Red Blood Cells ◽  
Hemolytic Anemia ◽  
Blood Cells ◽  
Antihypertensive Drugs ◽  
Drug Induced ◽  
Main Method ◽  
Hereditary Risk ◽  
Common Drug ◽  
Severity Of The Disease ◽  
Glucose 6 Phosphate Dehydrogenase

One of the reasons for the development of hemolytic anemia (HA) can be drugs, including some antibacterial, non-steroidal anti-inflammatory, antitumor and antihypertensive drugs. It was found that the most common drug-induced hemolytic anemia (DIHA) develops against the background of taking antibacterial drugs. The true prevalence of DIHA is not known and is approximately one case per 1.0–1.2 million patients. The mechanisms of the occurrence of DIHA are divided into immune and metabolic (non-immune). The first mechanism is associated with the formation of haptens, the second option – with the formation of immune complexes, the third option is mediated by the formation of true autoantibodies to red blood cells, the fourth option of the immune mechanism of the occurrence of DIHA is non-immunological protein absorption on the membranes of red blood cells. The risk factors for the development of DIHA are not fully established. The most common hereditary risk factor for DIHA is glucose-6-phosphate dehydrogenase deficiency. The main method of diagnosing DIHA is a direct antiglobulin test (direct Coombs’ test). The temporal relationship between the use of the inducer drug and the development of HA symptoms is important. The treatment strategy of DIHA is determined by the severity of the disease. In all cases, treatment should be initiated with the identification and withdrawal of the drug that initiated the occurrence of HA. With the development of severe HA, hemodialysis may be required. Prevention of DIHA involves avoiding the use of drugs associated with a high risk of its development.

scholarly journals Assessment of the tissue respiration intensity after cardiopulmonary bypass by studying erythrocytes morphology

2019 ◽  
Vol 18 (3) ◽  
pp. 53-58
Author(s):  
D. Yu. Romanovskiy ◽  
A. M. Volkov ◽  
A. V. Biryukov ◽  
I. R. Skibro ◽  
A. G. Butuzov ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
Red Blood Cells ◽  
Morphometric Analysis ◽  
Blood Cells ◽  
Blood Test ◽  
Heart Surgery ◽  
Tissue Respiration ◽  
Gas Exchanges ◽  
Respiration Intensity ◽  
Arterial Blood

Introduction. It was previously unknown that the form of erythrocytes depends on their interaction with oxygen. The form of red blood cells is reversible and changes both after passing through the lungs (oxygenator), and after gas exchanges in the tissues.Purpose of the study. In order to study the effect of cardiopulmonary bypass (CPB) on the erythrocyte morphology, the intensity of gas exchanges in the body tissues of the patient was determined using morphometric analysis of the form of erythrocytes.Material and methods. To achieve this goal, we developed a method for the morphometric analysis of erythrocytes «in vitro». Blood test was performed before surgery, on the 10th and every 30th minute of CPB, 12 hours after surgery.Results. Using this fact, we studied tissue respiration intensity. We found that normal arterial blood contains 85 % [80–95 %] red blood cells covered with small «villi» (0.3–0.4 μm), whereas venous blood consists mostly of «large-villous» erythrocytes (length of the «villi» 0.4–1.0 μm, 70 % [6–80 %]). During pairwise comparison we found the significant difference in the proportion of «large-villous» red blood cells in arterial blood before (15 % [5–20 %]) and after (35 % [20–50 %]) CPB. Partial pressure of oxygen and carbon dioxide changes throughout CPB and it shows changes in oxygen carrying capacity of blood. Decrease in the oxygen capacity of the blood reflects the intensity of tissue respiration on the one hand, and the degree of mechanical damage to red blood cells on the other.Conclusion. Heart surgery in infrared conditions lead to pronounced shifts in the ratio of discocytes and pathological forms of red blood cells in the blood. Inhibition of the red blood oxygen consumption during CPB indicates changes in the metabolic reactions, erythrocytes morphology and functionality. These data give us more complete understanding of the pathophysiological changes throughout CPB. The proposed method of erythrocytes morphometric analysis can be used as an express blood test in heart surgery with CPB, enabling better understanding of red blood state.

Monocytosis in rational empirical antibacterial therapy in moderate forms of COVID-19

2021 ◽  
Vol 2 (3(5)) ◽  
pp. 35-40
Author(s):  
A.V. Kovalev ◽  
◽  
M.I. Shperling ◽  
A.S. Polyakov ◽  
Ya.А. Nоskov ◽  
...  
Keyword(s):  
Antibacterial Therapy ◽  
Blood Cells ◽  
White Blood Cells ◽  
Absolute Number ◽  
Glucocorticoid Therapy ◽  
Control Group ◽  
Blood Monocytes ◽  
Significant Difference ◽  
Empirical Antibacterial Therapy ◽  
The Absolute

Microbiologically confirmed bacterial co-infection occurs in 1.2%–7% of hospitalized patients with COVID-19. The study of rational approaches to empirical antibacterial therapy (ABT) of SARS-CoV-2 virus-induced pneumonia continues. Glucocorticoid (GCS) therapy, the main method for pathogenetic treatment of moderate forms of CОVID-19, can lead to the development of neutrophilic leukocytosis. The criterion for the differential diagnosis of leukocytosis could be determining the quantity of peripheral blood monocytes. Assessing the significance of identifying the monocyte quantity can serve as an additional criterion for assigning empirical ABT in the treatment of pneumonia caused by the new coronavirus infection. The aim of the study was to identify the characteristics of glucocorticoid-induced leukocytosis in patients with moderate COVID-19. The study included 86 patients with a confirmed diagnosis of COVID-19 (ICD codes: U07.1, U07.2) of moderate severity. The patients were divided into 2 groups. The comparison group consisted of 40 patients who were prescribed ABT after the manifestation of leukocytosis on the background of glucocorticoid therapy. The control group included 46 people who were not prescribed ABT after the manifestation of leukocytosis on the background of glucocorticoid therapy and until the end of their stay in the hospital. We compared the parameters of the clinical blood tests (the absolute number of white blood cells, neutrophils and monocytes (×109/L)) on days 3, 6 and 9 from the start of GCS therapy. As a result, on the 3rd day, both groups had neutrophilic leukocytosis (>9.0×109/L) and absolute monocytosis (>0.8×109/L). There was a statistically signif icant decrease in the absolute number of white blood cells, neutrophils and monocytes by days 6 and 9, compared with day 3 from the start of glucocorticoid therapy. When comparing blood parameters between the groups, there was no statistically significant difference in the number of cells on the 3rd, 6th and 9th day of GCS therapy (p>0.05). Glucocorticoid-induced leukocytosis is associated with absolute monocytosis. The administration of ABT in response to the occurrence of leukocytosis in this study did not affect the change in the level of white blood cells. At the same time, a likely factor in reducing these indicators was a decrease in the daily dosage of corticosteroids.

scholarly journals Effect of Intra- and Postoperative Fluid and Blood Volume on Postoperative Pulmonary Edema in Patients With Intraoperative Massive Bleeding: Multi-Center Cohort Study Using Time Varying Analysis

2020 ◽  
Author(s):  
Young Suk Kwon ◽  
Jun Woo Choi ◽  
Hanna Lee ◽  
Jong Ho Kim ◽  
Ji Su Jang ◽  
...  
Keyword(s):  
Cohort Study ◽  
Confidence Interval ◽  
Pulmonary Edema ◽  
Red Blood Cells ◽  
Blood Volume ◽  
Blood Cells ◽  
Medical Center ◽  
Massive Bleeding ◽  
Hazard Ratio ◽  
Time Varying

Abstract BackgroundIn patients with massive bleeding during surgery, the effect of intra- and postoperative fluid and blood volume on postoperative pulmonary edema is uncertain. The aim of this study is evaluating the occurrence risk relationship through time-varying analysis between postoperative pulmonary edema and intra- and postoperatively administered volume of fluid and blood in patients with intraoperative massive bleeding.MethodsThis study is a retrospective cohort study and data was obtained from the clinical data warehouse at Hallym University Medical Center, a multi-institutional data registry of 5 hospitals of Hallym University. Patients with intraoperative massive bleeding (≥40% of average blood volume) and who underwent a non-cardiac surgery at 5 hospitals between January 1, 2010, and December 31, 2019 were enrolled the study. The primary outcome was postoperative pulmonary edema occurrence within 72 hours after surgery. Secondary outcomes were postoperative pulmonary edema with hypoxemia. ResultsIn total, 2090 patients were included in the postoperative pulmonary edema analysis, and 300 patients developed pulmonary edema within 72 hours after surgery. The postoperative pulmonary edema hypoxemia analysis with hypoxia included 1660 patients; pulmonary edema with hypoxemia occurred in 161 patients. The increase in the amount of red blood cells/average blood volume/hour after surgery increased the risk of developing pulmonary edema after surgery (hazard ratio: 1.03, 95% confidence interval [1.01–1.05], P = 0.013) and the risk of developing pulmonary edema with hypoxemia (hazard ratio: 1.04, 95% confidence interval [1.01–1.07], P = 0.024).ConclusionIn this study, an increase in the transfusion of red blood cells per hour after surgery increased the risk of developing pulmonary edema after surgery. This increase can be considered a risk factor for the incidence of pulmonary edema.

scholarly journals Increased Frequency of Paroxysmal Nocturmal Hemoglobinuria Type Cells in Patients with Chronic Idiopathic Neutropenia Correlates with the Severity of the Disease and the Presence of Skewed T-Cell Expansions

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4778-4778
Author(s):  
Athena Damianaki ◽  
Elias Stagakis ◽  
Semeli Mastrodemou ◽  
Irene Mavroudi ◽  
Maria Velegraki ◽  
...  
Keyword(s):  
T Cell ◽  
Red Blood Cells ◽  
T Cell Activation ◽  
Blood Cells ◽  
Healthy Subjects ◽  
Cell Activation ◽  
Antibody Activity ◽  
Gene Repertoire ◽  
Chronic Idiopathic Neutropenia ◽  
Severity Of The Disease

Abstract Introduction: Chronic idiopathic neutropenia (CIN) of adults is an acquired disorder of granulopoiesis characterized by an unexplained, prolonged reduction in the number of peripheral blood (PB) neutrophils. We have previously shown that CIN pathophysiology is related to T-cell activation and increased apoptosis of granulocytic progenitor cells. Notably, in our cohort of patients we exclude cases with antineutrophil antibody activity, i.e. autoimmune neutropenia cases. Aim of the study: Preliminary data have shown the presence of minor populations of paroxysmal nocturmal hemoglobinuria (PNH) type cells in the PB of CIN patients. The present study aims to investigate further the presence of PNH type cells in the PB of a large cohort of patients fulfilling the diagnostic criteria of CIN and probe the possible association with the severity of the disease and the skewed T-cell profile. Patients - Methods: We have studied 91 adults with CIN and 55 hematologically healthy subjects, age- and sex-matched with the patients. We have used flow cytometry for the detection of PNH type cells according to the International Clinical Cytometry Society guidelines and for the identification of T-cell expansions based on the analysis of T-cell receptor beta variable (TRBV) gene repertoire. Results: CIN patients displayed increased proportion of PB PNH type FLAER-/CD24- neutrophils (0.05% ± 0.18%) and FLAER-/CD14- monocytes (1.33% ± 1.38%) compared to healthy individuals (0.005% ± 0.01% and 0.64% ± 0.66%, respectively; P=0.0044 and P=0.011, respectively). Similarly, the patients displayed increased proportion of both, type II CD235+/CD59dim and type III CD235+/CD59- (0.059% ± 0.29% and 0.065% ± 0.28%, respectively) PB PNH red blood cells, compared to healthy controls (0.01% ± 0.03% and 0.01% ± 0.03%, respectively; P<0.0001 and P< 0.0001, respectively). Interestingly, an inverse correlation was found between the proportion of PB PNH type neutrophils and the number of absolutely neutrophil counts in CIN patients (r=-0.2525, P=0.0157) whereas no correlation was found between the proportion of PNH red blood cells or monocytes and absolutely neutrophil counts. In accordance with our previously reported data, increased frequency of skewed T-cell expansions were identified in CIN patients (71.4%) compared to healthy subjects (29.9%; P<0.0001). PNH type neutrophils (FLAER-/CD24-) were identified at a higher frequency in patients with skewed TRBV repertoire (63.08%) compared to those with normal TRBV distribution (34.61%) (P = 0.0194) suggesting a possible pathogenetic/pathophysiologic association between T-cell activation and emergence of PNH neutrophils in CIN. Conclusion: CIN patients display minor populations of PNH type PB neutrophils, monocytes and red blood cells. The frequency of PNH type neutrophils is associated with the severity of neutropenia and it is higher among patients with skewed TRBV repertoire. These data support further the hypothesis that CIN displays common pathophysiologic features with immune-mediated bone marrow failure syndromes and should be included in this spectrum of disease entities. Disclosures Papadaki: Alexion Pharmaceuticals: Research Funding.

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