scholarly journals The effect of testosterone on the rate of oxygen consumption by prostate tissue

2018 ◽  
Vol 99 (5) ◽  
pp. 775-778
Author(s):  
A O Lobkarev ◽  
R Kh Khafiz’yanova ◽  
O A Lobkarev
Keyword(s):  
Oxygen Consumption ◽  
Chronic Prostatitis ◽  
Clinical Effectiveness ◽  
Prostatic Hyperplasia ◽  
Prostate Tissue ◽  
Male Rats ◽  
Rate Of Oxygen Consumption ◽  
Testosterone Administration ◽  
Air Tightness ◽  
Increasing Demand

Aim. To study the effect of testosterone on the rate of oxygen consumption by rodent prostate homogenate. Methods. The study included 30 healthy old white outbred male rats divided into two groups with 15 animals in each group. The rats of the first group were administered the application of 1 % testosterone-containing gel Androgel before the operation. The rats of the second group received no testosterone. Under anesthesia prostatectomy was performed. Homogenate was immediately prepared from each prostate. Further every homogenate was placed into 250 ml vial to determine the rate of oxygen consumption. Then the device measuring the concentration of oxygen dissolved in water was placed into the vial, and the air-tightness was created. Each vial was put into the thermostat for 30 minutes at 36.6 ˚C. Then the measurement of the concentration of O2 dissolved in the water was performed. Results. Application of transdermal gel with 1 % testosterone was found to cause increase of oxygen consumption by prostate tissue. This fact can explain why the clinical effectiveness of testosterone is individual to each patient with benign prostatic hyperplasia (BPH) and chronic prostatitis (CP): oxygen supply to the prostate is different in each patient with BPH and CP. So not in every patient the oxygen-transporting system is capable of supplying prostate tissues with the amount of oxygen according to increasing demand of the organ on testosterone administration. Conclusion. Testosterone increases the rate of oxygen consumption by prostate tissue.

scholarly journals Morphological features of hyperplastic prostate tissue in patients with chronic prostatitis

2020 ◽  
Vol 13 (4) ◽  
pp. 82-88
Author(s):  
I.A. Aboyan ◽  
◽  
A.N. Tolmachev ◽  
S.I. Lemeshko ◽  
◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Surgical Treatment ◽  
Chronic Prostatitis ◽  
Inflammatory Process ◽  
Morphological Changes ◽  
Prostatic Hyperplasia ◽  
Prostate Tissue ◽  
Hyperplastic Tissue ◽  
Laser Enucleation ◽  
Transvesical Adenomectomy

Introduction. Benign prostatic hyperplasia (BPH) is a common disease wich is characterized by the proliferation of periurethral prostate glandular tissue. Infectious and inflammatory processes in the prostate play an important role in proliferation and hyperplasia, causing a significant deterioration in prostate metabolism and proliferation acceleration. This leads to progressive growth of the hyperplastic tissue in the prostate gland (PG). Тhe aim of study was to perform a comparative analysis of morphological changes in prostate hyperplastic tissue in patients with chronic prostatitis, who underwent various types of surgical treatment for BPH. Materials and methods. A comparative analysis of morphological changes in prostate tissue was performed, using a material from 276 patients with chronic prostatitis and BPH, who underwent various types of surgical treatment. The patients were divided into three groups: group I (n = 50) - patients who underwent surgical transvesical adenomectomy, group II (n = 76) - patients who underwent transurethral resection of the prostate (TURP) and group III (n = 150) - men who underwent holmium laser enucleation of the prostate. Fragments of the removed prostate tissue were fixed with neutral 10% formalin. The tissue samples were numerated automatically, using DIAPATH Donatello processor. Microscopic examination was carried out using a light microscope ZEISS «Axioskop 40» (eyepiece x 10, objectives x 5, x 10, x 20, x 40, 100). Results. Morphological features of resected prostate from patients with BPH and from patients with its combination with chronic bacterial inflammation were analyzed. The morphological picture varies from acute purulent inflammation to pronounced chronic lymphoplasmacytic inflammation around the acini. The features of morphological changes in the prostate after transvesical adenomectomy, TURP and laser enucleation were also studied. It was shown, that against the background of a chronic infectious and inflammatory process as well as a gross cicatricial process in the prostate, the organ anatomy, as well as the architectonics of vessels are disrupted, which can lead to a decrease in surgical treatment effectiveness. Discussion. In the majority of patients with BPH histological examination reveals a lymphoplasmacytic reaction of varying severity, which causes anatomical abnormalities and microcirculation deterioration. This condition significantly increases the risk of postoperative complications, requiring long-term conservative therapy and sometimes another surgical intervention. Conclusions. Against the background of a chronic infectious and inflammatory process, a gross cicatricial process in the prostate, its anatomy as well as vessel architectonics become disrupted, leading to a decrease in the effectiveness of surgical treatment. In the comparative study of morphological characteristics after various types of surgical procedures, it was revealed that the least significant changes were detected after laser enucleation of prostatic hyperplasia.

scholarly journals The concentration of androgens and estrogens in benign prostatic hyperplasia

2021 ◽  
Vol 21 (4) ◽  
pp. 47-53
Author(s):  
G. E. Roitberg ◽  
K. G. Mkrtchyan ◽  
N. G. Kulchenko
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Blood Supply ◽  
Vena Cava ◽  
Reproductive Hormones ◽  
Prostatic Hyperplasia ◽  
Prostate Tissue ◽  
Male Rats ◽  
Control Group ◽  
Main Role ◽  
Study Objective

Background. The etiology of benign prostatic hyperplasia (BPH) has not been fully studied. The main role in the induction of prostate tissue proliferation is assigned to the metabolism of testosterone. Recently, it has been reported that one of the risk factors for BPH is a chronic violation of the blood supply to the prostate.The study objective is to determine the level of reproductive hormones in blood serum and prostate tissue when creating a model of chronic ischemia.Materials and methods. The model of chronic pelvic ischemia was created in 10 white non-linear mature rats by partial ligation of the inferior vena cava. The control group of the study consisted of 10 male rats of the same age. After 1.5 months, we performed a hormonal study in all rats (n = 20) determining the concentration of testosterone, dihydrotestosterone and estradiol in the blood and prostate tissue. Also, in all animals (n = 20), a morphological study of the prostate was performed.Results. We’ve found a significant increase in prostate mass in the main group of rats by 16.4 % (p <0.05). Animals with BPH and impaired blood supply to the prostate had changes in their hormonal status: increased levels of testosterone (p <0.05) and dihydrotestosterone (p >0.05) in the prostate tissue.Conclusion. Long-term ischemic disorders in the prostate may be a trigger factor for the development of BPH due to an increase in the concentration of testosterone.

Hypothalamic thermoregulatory pathways in the rat

1977 ◽  
Vol 43 (5) ◽  
pp. 770-777 ◽  
Author(s):  
T. M. Gilbert ◽  
C. M. Blatteis
Keyword(s):  
Blood Flow ◽  
Oxygen Consumption ◽  
Medial Forebrain Bundle ◽  
Male Rats ◽  
Cutaneous Blood Flow ◽  
Cutaneous Vasodilation ◽  
Rate Of Oxygen Consumption ◽  
Cutaneous Vasoconstriction ◽  
Cutaneous Blood

The cutaneous blood flow (mbl), rate of oxygen consumption (Vo2), rectal (Tre) and cutaneous (Tsk) temperatures, and shivering activity were measured in unanthetized male rats during a 2-h exposure to 26, 33, or 5 degrees C 2 wk after selective bilateral hypothalamic microknife cuts. Animals with preoptic-anterior hypothalamic (PO/AH) junction cuts 1.5 or 3.0 mm lateral to the midline, as well as parasagittal cuts which separated connections between the PO/AH and medial forebrain bundle exhibited a higher mbl at 26 degrees C than did sham-operated rats. At 5 degrees C the extended (3.0 mm) PO/AH cuts as well as the parasagittal cuts prevented cutaneous vasoconstriction but had no effect on shivering activity; hence Tre was not maintained. None of the cuts demonstrably impaired thermoregulation in the 33 degrees C environment. These results suggest that different sites in the hypothalamus may separately control cold-induced skin vasoconstriction and shivering activity, as well as heat-induced skin vasodilation. It would seem therefore that the integrity of the PO/AH is indispensable in rats for cold-induced cutaneous vasoconstriction but not for cold thermogenesis, and also not for heat-induced cutaneous vasodilation.

scholarly journals Recovery heat in muscular contraction without lactic acid formation

1931 ◽  
Vol 108 (757) ◽  
pp. 279-301 ◽  
Keyword(s):  
Acetic Acid ◽  
Oxygen Consumption ◽  
Lactic Acid ◽  
Muscular Contraction ◽  
Acid Formation ◽  
Anaerobic Conditions ◽  
Spontaneous Breakdown ◽  
Rate Of Oxygen Consumption ◽  
Resting Muscle

In a comparison of muscles poisoned with mono-iodo-acetic acid (IAA) in the presence and in the absence of oxygen respectively, Lundsgaard (1930) found:- (1) That the spontaneous breakdown of phosphagen in poisoned resting muscle is much more rapid under anaerobic conditions. (2) That the onset of the characteristic contracture produced by IAA is accompanied always by an increase in the rate of oxygen consumption.

Proximal tubule dysfunction in cystine-loaded tubules: effect of phosphate and metabolic substrates

1996 ◽  
Vol 271 (3) ◽  
pp. F717-F722
Author(s):  
G. Bajaj ◽  
M. Baum
Keyword(s):  
Oxygen Consumption ◽  
Proximal Tubule ◽  
Tricarboxylic Acid Cycle ◽  
Dimethyl Ester ◽  
Proximal Tubules ◽  
Intracellular Atp ◽  
Metabolic Substrates ◽  
Rate Of Oxygen Consumption ◽  
Intracellular Phosphate

Intracellular cystine loading by use of cystine dimethyl ester (CDME) results in a generalized inhibition in proximal tubule transport due, in part, to a decrease in intracellular ATP. The present study examined the importance of phosphate and metabolic substrates in the proximal tubule dysfunction produced by cystine loading. Proximal tubule intracellular phosphorus was 1.8 +/- 0.1 in control tubules and 1.1 +/- 0.1 nmol/mg protein in proximal tubules incubated in vitro with CDME P < 0.001). Infusion of sodium phosphate in rabbits and subsequent incubation of proximal tubules with a high-phosphate medium attenuated the decrease in proximal tubule respiration and prevented the decrease in intracellular ATP with cystine loading. Tricarboxylic acid cycle intermediates have been shown to preserve oxidative metabolism in phosphate-depleted proximal tubules. In proximal tubules incubated with either 1 mM valerate or butyrate, there was a 42 and 34% reduction (both P < 0.05) in the rate of oxygen consumption with cystine loading. However, tubules incubated with 1 mM succinate or citrate had only a 13 and 14% P = NS) reduction in the rate of oxygen consumption, respectively. These data are consistent with a limitation of intracellular phosphate in the pathogenesis of the proximal tubule dysfunction with cystine loading.

Viability and respiratory activity ofPseudomonas syringaecells starved in buffer

1995 ◽  
Vol 41 (4-5) ◽  
pp. 372-377 ◽  
Author(s):  
João P. S. Cabral
Keyword(s):  
Oxygen Consumption ◽  
Electron Transport ◽  
Pseudomonas Syringae ◽  
Respiratory Activity ◽  
Bacterial Cells ◽  
Intact Cells ◽  
Rate Of Oxygen Consumption ◽  
Metabolic Activities ◽  
Different Levels ◽  
Number Of Cells

Pseudomonas syringae cells starved in buffer released orcinol-reactive molecules and materials that absorbed ultraviolet light. The number of cells culturable in nutrient medium decreased more rapidly than the number of intact particles determined by microscopy. The results suggested that starvation resulted in the lysis of an increasing number of cells, and that a fraction of the intact particles were not culturable. Starvation also resulted in a decrease in the rate of oxygen consumption with acetate, glycerol, and succinate, but at different levels. Whereas the respiration of acetate and glycerol decreased concomitantly with culturability, the respiration of succinate decreased to levels similar to the concentration of intact cells, suggesting that all intact particles respired the succinate, but only the culturable cells respired the acetate and glycerol. The results suggest that measuring the activity of the electron-transport system can overestimate the viability of starved bacterial cells, and that complex metabolic activities such as the respiration of acetate and glycerol are probably better suited for the evaluation of this parameter.Key words: Pseudomonas syringae, starvation, culturability, viability, respiration.

scholarly journals EFFECTS OF AZIDE AND CHLORETONE ON THE SODIUM AND POTASSIUM CONTENTS AND THE RESPIRATION OF FROG SCIATIC NERVES

1958 ◽  
Vol 41 (5) ◽  
pp. 959-988 ◽  
Author(s):  
W. P. Hurlbut
Keyword(s):  
Oxygen Consumption ◽  
Respiratory Depression ◽  
Conduction Block ◽  
Slow Conduction ◽  
Ionic Distribution ◽  
Rate Of Oxygen Consumption ◽  
Sciatic Nerves ◽  
Time Courses ◽  
Sodium And Potassium

Azide (0.2 to 5.0 mM) and chloretone (2.0 to 15.0 mM) reversibly inhibited 20 to 90 per cent of the resting respiration of frog sciatic nerves, and caused a loss of potassium and a gain of sodium in this tissue. The changes in ionic contents that developed after 5 or 10 hours were roughly correlated with the degree of respiratory depression, but the time courses of these changes were different with the two reagents. In azide these changes appeared to begin immediately, while in chloretone, at concentrations between 3.0 and 5.0 mM, the ionic shifts developed after a delay of several hours. Fifteen millimolar chloretone produced immediate changes in ionic contents several times greater than those produced by anoxia. The changes in ionic distribution produced in 5 hours by anoxia, 5.0 mM azide, or 5.0 mM chloretone were at least partially reversible; those produced by 15.0 mM chloretone were irreversible. With the exception of 15.0 mM chloretone the ionic shifts produced by these reagents may be due primarily to the depression of the respiration, although there are indications that azide acts, in addition, by another pathway. Concentrations of azide or chloretone that depressed the resting rate of oxygen consumption more than 50 per cent produced a slow conduction block, while 15.0 mM chloretone blocked conduction within 15 minutes.

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