scholarly journals Study of metabolic disorders in rats under exposure to hypobaric hypoxia and development of ­correction approaches by simultaneous action on different elements of pathogenesis

2021 ◽  
Vol 102 (5) ◽  
pp. 654-662
Author(s):  
I I Semina ◽  
A Z Baychurina ◽  
E V Shilovskaya ◽  
N E Tikhonova ◽  
D O Nikitin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Frontal Cortex ◽  
Hypobaric Hypoxia ◽  
Creatine Phosphokinase ◽  
Serum Alkaline Phosphatase ◽  
Statistical Significance ◽  
Brain Structures ◽  
Pressure Chamber ◽  
Simultaneous Action

Aim. To study the indicators of metabolic changes in the blood and brain structures of rats after exposure to hypobaric hypoxia and to determine possible pharmacological approaches to correction these changes. Methods. Hypobaric hypoxia in rats was simulated for 30 minutes in a pressure chamber, simulating an ascent to 8500 m. 3 and 24 hours after hypoxia, the activity of alanine aminotransferase, aspartic aminotransferases, alkaline phosphatase, creatine phosphokinase, lactate dehydrogenase, the content of glucose, total protein, triglycerides, cholesterol, -lipoproteins, iron and uric acid were determined in the blood serum. The level of malondialdehyde in the hippocampus and frontal cortex was examined. The studies of the effect of 2-chloroethoxy-aryl-dimethyl-aminophenylphosphorylacetohydrazide (CAPAH) (1 mg/kg) and Рiracetam (100 mg/kg) after intraperitoneal injection 40 minutes before hypoxia and 1 hour after removing the rats from the pressure chamber were carried out. Statistical analysis was carried out using the GraphPad Prism software version 8.0.1, and the Student's t-test was used to test statistical significance. Results. After 3 hours of hypobaric hypoxia, rats showed hyperenzymemia and dyslipidemia, the activity of almost all studied enzymes in the blood serum of rats was increased, the content of triglycerides was decreased, and the concentration of cholesterol was increased, the content of malondialdehyde in the hippocampus and frontal cortex was increased. In 24 hours after hypoxia, an increased level of creatine phosphokinase in the blood serum and malondialdehyde in the brain structures were noted. The use of 2-chloroethoxy-aryl-dimethyl-aminophenylphosphorylacetohydrazide prevented the development of hyperenzymemia, dyslipidemia and corrected the increased level of creatine phosphokinase after 24 hours; in both modes of administration, it reduced the serum level of malondialdehyde. Piracetam showed little effect only when administered prophylactically, preventing an increase in serum alkaline phosphatase activity and cholesterol levels. Сonclusion. The revealed efficacy of 2-chloroethoxy-aryl-dimethyl-aminophenylphosphorylacetohydrazide and its previously studied complex mechanism of action suggest that 2-chloroethoxy-aryl-dimethyl-aminophenylphosphorylacetohydrazide is a potential drug for the prevention of hypoxic disorders and acceleration of adaptation to high-altitude hypoxia.

The activity of alkaline phosphatase in the blood of purebred and crossbred heifers

2020 ◽  
pp. 13-20
Author(s):  
R. Milushev ◽  
V. Epifanov
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Live Weight ◽  
Milk Productivity ◽  
Holstein Breed ◽  
Lactating Cows ◽  
Adaptive Capabilities ◽  
Age Related ◽  
Productive Traits

Simmental breed of cattle combines dairy and beef productivity. The crossbreeding cows of Simmental breed with Red-and-White Holstein breed of cattle was supposed to obtain animals with high milk productivity, good adaptability to the industrial technology of milk productivity. Red-and-White breed of cows has been bred in Russia as a result of this crossbreeding. It is a relatively young breed of dairy productivity in our country. In order to evaluate the effect of crossbreeding and study the adaptive capabilities obtained by animals, the synthesis of alkaline phosphatase by its activity in blood serum has been studied. The level of activity of the enzyme reflected the intensity and direction of biochemical processes in animals, affecting the state of homeostasis in purebred (Simmental) and crossbred (F1 Simmental×Red-and-White Holstein) heifers and first-calf heifers. It has been found that activity of serum alkaline phosphatase has decreased with age in both purebred and crossbred animals. Crossbreds F1 surpassed purebred heifers in enzyme activity at 1 and 10 months of age, this difference was significant. The activity of the enzyme in first-calf heifers was different from that in the blood of growing heifers, they had it lower. A close and significant correlation has been found between the live weight of growing crossbred heifers and the activity of alkaline phosphatase in the blood serum at 2-, 6- and 12-month age. Crossbred first-calf heifers were significantly higher in milk yield for 90 days of lactation than their Simmental herdmates. The set of data on the activity of alkaline phosphatase in the blood of heifers and lactating cows is of both theoretical and practical value for zootechnical and biological sciences, revealing the regularities of the heterosis effect, deepening knowledge about the age-related biochemistry of crossbred animals and the formation of productive traits.

Activity of alkaline phosphatase in male lamb blood serum in relation to age and growth rate

2021 ◽  
pp. 9-16
Author(s):  
Y. Zharikov ◽  
L. Kaneva
Keyword(s):  
Growth Rate ◽  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Live Weight ◽  
Growth Potential ◽  
Age And Growth ◽  
Serum Alkaline Phosphatase Activity ◽  
Growth Intensity ◽  
Lower Productivity

Purpose: to assess the correlation of serum alkaline phosphatase with the live weight and growth rate of male lambs during the milk and post-milk growing periods.Materials and methods. The study of indicators of serum alkaline phosphatase activity in 74 male lambs aged one and five months was carried out in connection with the intensity of growth and the peculiarities of metabolism. All the male lambs were born at the same time and were raised for up to six months under similar feeding and maintenance conditions. To study the dynamics of changes in the live weight of young animals, individual weighing was carried out monthly with an accuracy of 0.1 kg. Based on the results of weighing, the absolute, relative, and average daily live weight gains were calculated and the animals were divided into three groups. The analysis of the serum for the content of the studied parameters was performed by photometry with the help of the corresponding sets of reagents of the company «VitalDiagnostics SPb».Results. During the observation period, the average increase in live weight of male lambs was 21,14 kg, or 136,75 g per day. At one month of age, the activity of alkaline phosphatase was 188,61±9.50 U/l, and at five months — 100,01±3,58, or 47% (P<0,001) lower. It was found that the activity of the enzyme during the suckling period was positively associated with live weight and growth intensity (r=0,35 and 0,49), and after weaning from mothers — negatively (r = -0,46 and -0,46, respectively). The activity of alkaline phosphatase was consistently increased in monthly male lamb when they moved from a group with lower productivity to a larger one — 160,5 — 191,4 — 214,9, and in five-month-olds, on the contrary, decreased — 121,0 — 98,1 — 80,2 E/l. At the age of one month, the increase in growth intensity was accompanied by an increase in the blood serum of male lamb values of gamma-glutamyltranspeptidase, glucose, calcium and phosphorus, and a decrease in urea and cholesterol levels. At the age of five months, both the list of links and their vector changed. In the serum of fast-growing animals, the de Ritis coefficient and gamma-glutamyltranspeptidase were higher, but the activity of lactate dehydrogenase and creatine phosphokinase was lower.Conclusion. The presence of a correlation between alkaline phosphatase and growth makes it possible to use alkaline phosphatase in zootechnical practice and breeding work as an additional criterion for the growth potential of male lambs up to six months in the early stages of ontogenesis. However, the change in the nature of the relationship from plus to minus implies a differentiated approach in the use of this indicator, taking into account the age of the male lambs.

STUDIES ON THE PERFORMANCE, DEVELOPMENT AND CARCASS COMPOSITION OF THE GROWING PIG: EFFECTS OF SEX, FEEDING REGIME AND AGE ON BLOOD SERUM PARAMETERS

1983 ◽  
Vol 63 (4) ◽  
pp. 977-984 ◽  
Author(s):  
H. DOORNENBAL ◽  
A. K. W. TONG ◽  
A. H. MARTIN ◽  
A. P. SATHER
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Age Groups ◽  
Inorganic Phosphorus ◽  
Carcass Composition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Parameters ◽  
Feeding Regime

Blood samples from 120 pigs serially slaughtered at five age groups, starting at 85 days of age with intervals of 2 wk, were used to study the effects of sex, feeding regime and age on a 12 component metabolic blood serum profile. The differences between barrows and gilts were small and only significant (P < 0.05) for blood urea. Pigs fed ad libitum had a significantly (P < 0.05) higher level of blood urea, calcium, inorganic phosphorus and alkaline phosphatase but a significantly (P < 0.01) lower level of creatinine than restricted fed pigs. Age had the most pronounced effects on the serum parameters studied, with blood urea, calcium, bilirubin, creatinine, total serum protein and albumin showing a significant (P < 0.01) increase per unit of serum with increasing age. The serum glucose, inorganic phosphorus, glutamic oxaloacetic transaminase and lactate dehydrogenase were also significantly (P < 0.01, P < 0.05 for glucose) influenced by age but did not show a consistent trend across the age groups. Serum alkaline phosphatase was the only component which significantly (P < 0.01) decreased with age. The results indicate that when these serum components are to be used for diagnostic or comparison purposes, age adjustment must be applied. Key words: Blood parameters, pigs, age, feeding regime, sex

Zinc Deficiency in Reproducing Gilts Fed a Diet high in Calcium and its Effect on Tissue Zinc and Blood Serum Alkaline Phosphatase

1967 ◽  
Vol 26 (6) ◽  
pp. 1348-1357 ◽  
Author(s):  
W. G. Hoekstra ◽  
E. C. Faltin ◽  
C. W. Lin ◽  
H. F. Roberts ◽  
R. H. Grummer
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Zinc Deficiency ◽  
Serum Alkaline Phosphatase

scholarly journals Reference Value of Blood Serum Alkaline Phosphatase in Mongolian Adult

2014 ◽  
Vol 17 (7) ◽  
pp. A752
Author(s):  
G. Chanagsuren ◽  
E. Dorj ◽  
O. Dorj ◽  
B. Gunchin
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Reference Value

The Origin of Elevation of Serum Alkaline Phosphatase in Hepatic Disease

1962 ◽  
Vol 42 (4) ◽  
pp. 431-438 ◽  
Author(s):  
Stanton G. Polin ◽  
Mitchell A. Spellberg ◽  
Lloyd Teitelman ◽  
Makoto Okumura
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Hepatic Disease

SUBACUTE THYROIDITIS WITH ELEVATED SERUM ALKALINE PHOSPHATASE LEVEL

1960 ◽  
Vol XXXIV (II) ◽  
pp. 256-260
Author(s):  
Jörgen Herman Vogt
Keyword(s):  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Level ◽  
Serum Alkaline Phosphatase ◽  
Thyroid Tissue ◽  
Elevated Serum ◽  
Subacute Thyroiditis ◽  
Serum Alkaline Phosphatase Level ◽  
Transient Rise

ABSTRACT A case of subacute thyroiditis is recorded, in which a transient rise in serum alkaline phosphatase values leads to the hypothesis of a transient parathyroid hyper-activity induced by the inflammation of the thyroid tissue in which the parathyroid may be embedded.

The validity of serum alkaline phosphatase to identify nutritional rickets in Nigerian children on a calcium-deprived diet

2019 ◽  
Author(s):  
Tom Thacher ◽  
Christopher Sempos ◽  
Ramon Durazo-Arvizu ◽  
Craig Munns ◽  
Philip Fischer ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Nigerian Children ◽  
Nutritional Rickets
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