scholarly journals Ultrasonographic Evaluation of Hip Joint in Newborns

2003 ◽  
Vol 10 (4) ◽  
pp. 23-27
Author(s):  
H A Es'kin ◽  
L K Mikhaylova ◽  
N A Es'kin ◽  
L K Mikhailova

Two hundred fifty newborns (ПО girls and 140 boys) were examined with ultrasonography. The method of acetabular index measurement was used to assess the hip joint status. High efficacy of sonography in differentiation of true and false-positive Marks-Ortholani symptom was noted. High correlation between sonography data and radiography ones was detected. Absence of irradiation, possibility of examination in real time and repeated examinations make sonography one of the main methods for hip joint screening.

Author(s):  
Khuushi ◽  
Vanadana Jain ◽  
Rajendra Patrikar ◽  
Raghavendra Deshmukh

2021 ◽  
Author(s):  
Vandana Kuttappan Nair ◽  
Chandrika Sharma ◽  
Mrittika Sengupta ◽  
Souradyuti Ghosh

<b>Layman Summary: </b>Rolling circle amplification (RCA) is a popular and extensively used bioanalytical tool. Like any nucleic acid amplifications, non-specific amplification may occur in it and risk generating false positive readouts. The work described in the manuscript investigates non-specific amplification in RCA as a function of ligation and exonuclease digestion assays during the synthesis of circular DNA. In particular, it investigates and compares the role of three different ligation techniques, namely splint-padlock ligation, cohesive end (sticky end ligation), and self-annealing ligation. In addition, it also probes the role of single exonuclease vs dual exonuclease digestions. We employed real time fluorescence to quantify the effect of these factors. Finally, our work hypothesizes the possible origins of non-specific amplification in RCA.


2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Viktor Szatmári ◽  
Martin Willem van Leeuwen ◽  
Christine Jantine Piek ◽  
Luigi Venco

Abstract Background Dirofilaria immitis is responsible for heartworm disease in dogs in endemic areas worldwide. Screening for this infection is done by blood tests. Antigen testing is the most sensitive method to detect an infection with adult (female) worms. Microscopic examination of a blood smear or Knott’s test can be used to detect circulating microfilariae, the infective larvae. To increase the sensitivity of the antigen test by decreasing the false negative test results, heating of the blood sample has been recommended in recent guidelines. Heating is believed to remove blocking immune-complexes. Circulating microfilariae are not specific findings for heartworm infection, as other nematodes (among others, Acanthocheilonema dracunculoides) can also result in microfilaremia. Although the type of microfilariae cannot be determined by microscopy alone, real-time PCR can reliably identify the infecting nematode species. Correct identification of the parasite is of major importance, as an infection with D. immitis requires antiparasitic therapy, whereas A. dracunculoides is thought to be a clinically irrelevant coincidental finding. The present case report describes a microfilaremic dog where the initial antigen test for D. immitis turned positive after heat treatment, whereas real-time PCR revealed that the microfilariae were A. dracunculoides (syn. Dipetalonema dracunculoides). Results A circa 5-year old, asymptomatic Spanish mastiff dog was referred for heartworm therapy because microfilariae were found via a screening blood test. The dog was recently imported to the Netherlands from Spain, where it had been a stray dog. Antigen tests on a plasma sample for D. immitis were performed with three different test kits, which all turned out to be negative. However, heat treatment of two of these samples were carried out and both of them led to a positive antigen test result. Real-time PCR showed that the circulating microfilariae belonged to A. dracunculoides species. Three administrations of moxidectin spot-on at monthly intervals resulted in a negative antigen and a negative Knott’s tests one month after the last treatment. Conclusions We conclude that heat treatment of initially negative blood samples for D. immitis could lead to false positive antigen test results if the dog is infected with A. dracunculoides.


Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4383
Author(s):  
Shafique Ahmed ◽  
Thomas Schumacher ◽  
Erik T. Thostenson ◽  
Jennifer McConnell

This article describes research that investigated the ability of a carbon nanotube (CNT) sensor to detect and monitor fatigue crack initiation and propagation in metal structures. The sensor consists of a nonwoven carrier fabric with a thin film of CNT that is bonded to the surface of a structure using an epoxy adhesive. The carrier fabric enables the sensor to be easily applied over large areas with complex geometries. Furthermore, the distributed nature of the sensor improves the probability of detecting crack initiation and enables monitoring of crack propagation over time. Piezoresistivity of the sensor enables strains to be monitored in real time and the sensor, which is designed to fragment as fatigue cracks propagate, directly measures crack growth through permanent changes in resistance. The following laboratory tests were conducted to evaluate the performance of the sensor: (1) continuous crack propagation monitoring, (2) potential false positive evaluation under near-threshold crack propagation conditions, and (3) crack re-initiation detection at a crack-stop hole, which is a commonly used technique to arrest fatigue cracks. Real-time sensor measurements and post-mortem fractography show that a distinguishable resistance change of the sensor occurs due to fatigue crack propagation that can be quantitatively related to crack length. The sensor does not show false positive responses when the crack does not propagate, which is a drawback of many other fatigue sensors. The sensor is also shown to be remarkably sensitive to detecting crack re-initiation.


2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Natalia Bulgakova ◽  
Victor Sokolov ◽  
Larisa Telegina ◽  
Konstantin Vereshchagin ◽  
Georgy Frank ◽  
...  

AbstractAutofluorescence bronchoscopy (AFB) is currently considered the most promising diagnosis modality for the detection of early stage lung cancer with a sensitivity which normally exceeds 90%, although its specificity is rather low. The number of false positive results can be reduced by the combination of autofluorescence (AF) imaging with quantitative spectroscopy. The aim of this study was to reveal additional spectral characteristics of AF emission which may have the potential to maximize the endogenous contrast between normal mucosa and bronchial malignancyAF emission spectra were recorded at 532 nm laser excitation in the course of AFB of totally 20 patients with central-type X-ray negative lung cancer. According to the results of histopathological analysis, two sets were made up of the spectral data corresponding to normal bronchial epithelium and malignant lesions.Comparative studies of nine basic spectral characteristics ofReal time measurements and estimation of all important spectral information on the basis of the probabilistic approach is assumed to be useful for real time recommendations for biopsies and for minimizing the number of false positive results during the course of AFB. For the realization of this approach, further trials are necessary to collect a large number of AF spectra samples, corresponding to different tissue pathologies such as inflammation, metaplasia, and dysplasia.


Apmis ◽  
2009 ◽  
Vol 117 (1) ◽  
pp. 68-72 ◽  
Author(s):  
FOROUGH L. NOWROUZIAN ◽  
INGEGERD ADLERBERTH ◽  
AGNES E. WOLD

2021 ◽  
Vol 9 (3) ◽  
pp. 519-537
Author(s):  
Hossein Ghaffarian ◽  
Pierre Lemaire ◽  
Zhang Zhi ◽  
Laure Tougne ◽  
Bruce MacVicar ◽  
...  

Abstract. Wood is an essential component of rivers and plays a significant role in ecology and morphology. It can be also considered a risk factor in rivers due to its influence on erosion and flooding. Quantifying and characterizing wood fluxes in rivers during floods would improve our understanding of the key processes but are hindered by technical challenges. Among various techniques for monitoring wood in rivers, streamside videography is a powerful approach to quantify different characteristics of wood in rivers, but past research has employed a manual approach that has many limitations. In this work, we introduce new software for the automatic detection of wood pieces in rivers. We apply different image analysis techniques such as static and dynamic masks, object tracking, and object characterization to minimize false positive and missed detections. To assess the software performance, results are compared with manual detections of wood from the same videos, which was a time-consuming process. Key parameters that affect detection are assessed, including surface reflections, lighting conditions, flow discharge, wood position relative to the camera, and the length of wood pieces. Preliminary results had a 36 % rate of false positive detection, primarily due to light reflection and water waves, but post-processing reduced this rate to 15 %. The missed detection rate was 71 % of piece numbers in the preliminary result, but post-processing reduced this error to only 6.5 % of piece numbers and 13.5 % of volume. The high precision of the software shows that it can be used to massively increase the quantity of wood flux data in rivers around the world, potentially in real time. The significant impact of post-processing indicates that it is necessary to train the software in various situations (location, time span, weather conditions) to ensure reliable results. Manual wood detections and annotations for this work took over 150 labor hours. In comparison, the presented software coupled with an appropriate post-processing step performed the same task in real time (55 h) on a standard desktop computer.


Author(s):  
Yu. N. Gavrik ◽  
Yu. Yu. Markelova ◽  
Yu. O. Kuzmina

Introduction. Hip dysplasia in infants is one of the most common congenital defects of connective tissue. It is assumed that correction of somatic dysfunctions, which are formed in this orthopaedic pathology, will affect the timing of treatment and rehabilitation.Goal of research — to explore the opportunities of osteopathic correction for somatic dysfunctions in comprehensive conservative treatment of hip joint congenital orthopaedic pathology in young infants.Materials and methods. There were 40 infants under 6 month with diagnosis of hip joint dysplasia under observation, divided into two groups of 20 infants each. Functional orthopaedic treatment (massage, physiotherapy, exercise therapy, orthopaedic splint) and osteopathic correction were performed for infants of the main group. Infants of the control group received the orthopaedic treatment only. Osteopathic status was rated in addition to provided treatment in both groups. Treatment results were evaluated by using hip joints radiography, namely, by the acetabular index.Results. It was found that global somatic dysfunctions are not typical in infants with hip joint congenital defects. The most frequently identifi ed somatic dysfunctions were regional, in a region of head, neck, pelvis, as well as thoracic region. Somatic dysfunctions of head and pelvis region are the most signifi cant. It proves the functional connection between these dysfunctions and hip joint congenital defexts. X-ray examination data analysis let us suggest a direct effect of somatic dysfunctions correction on the acetabular index in infants with congenital orthopaedic pathology, and thus on rehabilitation periods reduction.Conclusion. The study showed that osteopathic correction of somatic dysfunctions is effective in combination with orthopaedic treatment of hip dysplasia performed from the fi rst months of infant′s life. It should be recommended as a part of comprehensive therapy of this orthopaedic pathology.


Sign in / Sign up

Export Citation Format

Share Document