Disturbances of Microcirculation and Antioxidant Potential as a Consequence of Ferrum Exchange in Traumatic Disease

2012 ◽  
Vol 19 (1) ◽  
pp. 64-69
Author(s):  
Aleksey Valer'evich Ivanov ◽  
Yu P Orlov ◽  
V N Lukach ◽  
T V Pritykina ◽  
A M Ivanova ◽  
...  
Keyword(s):  
Free Radical Oxidation ◽  
Blood Rheology ◽  
Traumatic Shock ◽  
Intravascular Hemolysis ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Bone Injury ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Fractures Of The Femur

Study of ferrum metabolism was performed in 30 patients with traumatic disease, i.e. fractures of the femur, pelvic bones, ribs accompanied by massive muscular hematomas. It has been detected that in severe bone injury besides extravascular hemolysis the intravascular hemolysis, that affects the ferrum metabolism, takes place. Excess of free ferrum activates free radical oxidation processes and results in reduction of total antioxidant activity that is proved by lability of Fe2+ - induced hemiluminescence indices as well as causes the disturbance of blood rheology that influences the course of traumatic disease. Results of experimental study on 30 male Wistar rats in which the conditions of I stage of traumatic shock were created showed that administration of Desferal contributed to blood viscosity improvement.

scholarly journals Critical conditions as a logical and appropriate chain of iron methabolism disorders (the summary of experimental studies)

2013 ◽  
Vol 59 (6) ◽  
pp. 700-709 ◽  
Author(s):  
Y.P. Orlov ◽  
V.N. Lukach ◽  
V.T. Dolgih ◽  
E.L. Soboleva ◽  
A.V. Ivanov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Rheological Properties ◽  
Experimental Studies ◽  
Free Radical Oxidation ◽  
Critical Conditions ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Total Antioxidant

It is revealed that in all models of critical conditions will activate the free-radical oxidation, decreasing the total antioxidant activity, the concentration of transferrin decreases in the serum of the blood, increase of the concentration of ferritin, the rheological properties of the blood are violated and the signs of endothelial dysfunction are identified. Pre-entered deferoxamine in the dose of 80 mg/kg reduced the intensity of free-radical oxidation processes, restoring the antioxidant potential, concentration of the transferrin, and a lower level of ferritin, contributed to the normalization of blood rheological properties and a reduction of the extent endothelium destruction as a result of the reduction Fe2+ concentration in blood serum.

scholarly journals Effect of Leu-Ile-Lys tripeptide on the activity of free radical oxidation and expression of сyclooxygenase-1 and -2 in gastric mucosa in experimental indomethacin-induced ulcer in rats

2019 ◽  
Vol 100 (3) ◽  
pp. 439-444
Author(s):  
S E Lorents ◽  
E N Mazko ◽  
O G Makarova ◽  
A Yu Zharikov ◽  
Yu V Korenovskiy ◽  
...  
Keyword(s):  
Free Radical ◽  
Gastric Mucosa ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Intragastric Administration ◽  
Radical Oxidation ◽  
Cyclooxygenase 1 ◽  
Total Antioxidant ◽  
Male Wistar Rats

Aim. To determine the effect of Leu-Ile-Lys tripeptide on indicators of oxidative stress and expression of cyclooxygenase-1 and -2 in the gastric mucosa on the background of experimental indomethacin-induced ulcer. Methods. Experiments were performed on 35 male Wistar rats with a body mass of 200-250 g aged 2-3 months that were divided into 3 groups: the control group (intact rats, 8 animals), control group (simulation of indomethacin-induced gastropathy, 12 animals) experimental group (simulation of indomethacin-induced gastropathy + administration of Leu-Ile-Lys tripeptide, 15 animals). The tripeptide Leu-Ile-Lys obtained by chemical synthesis (sample purity at least 98%) was administered intragastrically daily for 7 days before the simulation of indomethacin-induced damage of the gastric mucosa in a dose of 11.5 mg/kg. The model of indomethacin-induced damage to the rodent gastric mucosa was reproduced by a single intragastric administration of indomethacin in a dose of 60 mg/kg in 1 ml of saline. In the stomach homogenate the activity of free radical oxidation was determined by conventional methods. For quantitative determination of cyclooxygenase-1, -2 in gastric homogenate the method of enzyme immunoassay was used. The concentration was determined spectrometrically by the color intensity of the samples. Results. With a prophylactic administration of tripeptide Leu-Ile-Lys, concentration of tiobarbiturate-reactive products was normalized, total antioxidant activity increased and the activity of antioxidant enzymes decreased compared to the control group. The concentration of cyclooxygenase-2 in gastric homogenate from the experimental animals was 2.3 times lower than that of the control rats. Conclusion. Use of Leu-Ile-Lys tripeptide in experimental indomethacin-induced gastric ulcer showed a significant decrease of oxidative damage and inflammation in the gastric mucosa.

scholarly journals Influence of neuropeptides acth(4-7)-pro-gly-pro and acth(6-9)-pro-gly-pro on the intensity of redox reactions under experimental depression

2020 ◽  
Vol 10 (3) ◽  
pp. 29-32
Author(s):  
Marina Samotrueva ◽  
Anna Yasenyavskaya ◽  
Aleksandra Tsibizova ◽  
Jumazia Erizhepova ◽  
Nikolai Myasoedov ◽  
...  
Keyword(s):  
Free Radical ◽  
Social Stress ◽  
Redox Reactions ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Initial Content ◽  
The Brain

The experiment is devoted to the study of the antioxidant properties of neuropeptides from melanocortins ACTH(4-7)-Pro-Gly-Pro (Semax) and ACTH(6-9)-ProGly-Pro under conditions of experimental depression. The study was carried out on white outbred male rats. In the process of modeling experimental depression (social stress) inter-male confrontations were observed as a result of which groups of animals with aggressive and submissive behaviors were formed. The free radical oxidation processes were assessed by determining the activity of catalase, the initial content of malondialdehyde (MDA), the rate of spontaneous and ascorbate-dependent lipid peroxidation (LPO) in the hypothalamic and prefrontal regions of the brain by spectrophotometric method. It was found that under the influence of melanocortins, there is a pronounced suppression of the processes of free radical oxidation in the hypothalamic and prefrontal regions of the brain, which arose against the background of a stressful load which is manifested by a decrease in the indicators of the oxidative process.

scholarly journals CHARACTERISTIC OF FREE-RADICAL DAMAGE AND ANTIOXIDANT PROTECTION STATE IN PATIENTS WITH CHRONIC KIDNEY DISEASE AGAINST A BACKGROUND OF MALABSORPTION SYNDROME

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
А.Е. Shkarutyak
Keyword(s):  
Chronic Kidney Disease ◽  
Free Radical ◽  
Kidney Disease ◽  
Free Radical Oxidation ◽  
Oxidation Products ◽  
Essential Factor ◽  
Free Radical Damage ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Radical Damage

Oxidation processes play an important role in damage of the renal structures, especiallyagainst a background of other serious pathologies, in particular, malabsorptionsyndrome.The study of the mechanisms of mutual aggravation and progression of kidneydamage against a background of malabsorption is a very urgent problem of the presenttime.Purpose - to investigate the state of free radical oxidation of lipids, proteins andantioxidant defense in patients with chronic kidney disease of different ages withoxaluria against a background of malabsorption syndrome.Material and methods. 98 patients with chronic kidney disease of the I-II stages(pyelonephritis) with the presence of oxaluria against a background of malabsorptionsyndrome of different age were examined.The age of the patients under examination was32 - 64. All patients underwent a study of the system of free radical oxidation of lipidsand proteins.Results. chronic kidney disease with the presence of oxaluria is accompanied by asignificant increase in the blood content of free radical oxidation products, namelyaldehyde and ketone dinitrophenylhydrazones, especially of a neutral nature,malondialdehyde, as well as a significant decrease in the indicators of the antioxidantdefense system, which manifested itself to a greater extent in patients with older age.groups.Conclusion. An essential factor in the development and progression of chronic kidneydisease with the presence of oxaluria against a background of malabsorption syndromeis an increase in the intensity of free radical oxidation of lipids and proteins (mainly dueto aldehyde and ketondinitrophenylhydrazones of a neutral nature).

scholarly journals Effects of His-Phe-Arg-Trp-Pro-Gly-Pro peptide on free-radical oxidation processes in conditions of chronic restraint stress

2021 ◽  
Author(s):  
AO Vorvul ◽  
II Bobyntsev ◽  
OA Medvedeva ◽  
IuE Azarova ◽  
AE Belykh ◽  
...  
Keyword(s):  
Free Radical ◽  
Restraint Stress ◽  
Free Radical Oxidation ◽  
Regulatory Peptides ◽  
Thiobarbituric Acid ◽  
Synthetic Analog ◽  
Chronic Restraint Stress ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Wide Range

Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. Currently, a wide range of peptide neurotropic drugs, affecting the stress response development, are used in medicine, and new promising molecules are being studied. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. Serum levels of 8-oxo-2'-deoxyguanosine (8-OHdG) and superoxide dismutase 3 (SOD3) were assessed by enzyme immunoassay, and the levels of thiobarbituric acid reactive substances (TBARS) were assessed by fluorimetric method. CRS lead to the significant increase in the 8-OHdG levels by 18.4% (p = 0.01) and the decrease in the SOD3 levels by 14.3% (p = 0.01), however, it had no effect on the levels of TBARS. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. Thus, it was found that CRS resulted in oxidative stress in animals. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes.

scholarly journals THE USE OF PLASMAPHERESIS IN TREATMENT OF PATIENTS WITH INFERTILITY, PERITONEAL ENDOMETRIOSIS AND NAT2 GENE POLYMORPHISM

2016 ◽  
Vol 2 ◽  
pp. 3-10 ◽  
Author(s):  
Ekaterina Dubinskaya ◽  
Natalia Lapteva ◽  
Yana Lukyanova
Keyword(s):  
Oxidative Stress ◽  
Point Mutations ◽  
Free Radical Oxidation ◽  
Blood Rheology ◽  
Comprehensive Treatment ◽  
Radical Oxidation ◽  
Treatment Techniques ◽  
Peritoneal Endometriosis ◽  
Nat2 Gene ◽  
And Oxidative Stress

It is known that 30–40 % of patients with peritoneal endometriosis suffer from infertility. Half of the patients with endometriosis are identified point mutation in NAT2 – gene, which plays an important role in the acetylation of aromatic and heterocyclic amines, in the accumulation of endotoxins, activation of free radical oxidation, impaired microcirculation. These factors involve the use of methods of gemapheresis which have detoxification, the blood rheology corrective and immune corrective effects. The purpose of this study was to evaluate the efficacy of therapeutic plasma exchange in treatment of patients with peritoneal form of endometriosis, infertility and point mutations in the gene NAT2. The study included 140 patients with infertility, peritoneal form of endometriosis and point mutations in the gene NAT2. All patients are performed laparoscopy, coagulation foci of endometriosis. In the following 93 (66.4 %) patients were treated with a the course of therapeutic plasmapheresis using the apparatus «PCS-2» with the removal of 20–25 % the volume of circulating plasma with replacement plasma of crystalloid and colloid solutions. Before treatment were shown the signs of endotoxemia, activation of oxidative stress. After treatment with the use of plasmapheresis was revealed the significant reduction of endogenous intoxication parameters and oxidative stress. Also is noted the increase in the pregnancy rate, both independently and in IVF programs, especially during the first 3 months after treatment. The findings suggest that the efficiency of the proposed comprehensive treatment techniques (laparoscopy and subsequent course of therapeutic plasmapheresis) of patients with peritoneal endometriosis and infertility and with point mutations in the gene NAT2. The use of plasmapheresis is pathogenetically justified in patients of the studied group.

Sign in / Sign up

Export Citation Format

Share Document