scholarly journals Influence of neuropeptides acth(4-7)-pro-gly-pro and acth(6-9)-pro-gly-pro on the intensity of redox reactions under experimental depression

2020 ◽  
Vol 10 (3) ◽  
pp. 29-32
Author(s):  
Marina Samotrueva ◽  
Anna Yasenyavskaya ◽  
Aleksandra Tsibizova ◽  
Jumazia Erizhepova ◽  
Nikolai Myasoedov ◽  
...  
Keyword(s):  
Free Radical ◽  
Social Stress ◽  
Redox Reactions ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Initial Content ◽  
The Brain

The experiment is devoted to the study of the antioxidant properties of neuropeptides from melanocortins ACTH(4-7)-Pro-Gly-Pro (Semax) and ACTH(6-9)-ProGly-Pro under conditions of experimental depression. The study was carried out on white outbred male rats. In the process of modeling experimental depression (social stress) inter-male confrontations were observed as a result of which groups of animals with aggressive and submissive behaviors were formed. The free radical oxidation processes were assessed by determining the activity of catalase, the initial content of malondialdehyde (MDA), the rate of spontaneous and ascorbate-dependent lipid peroxidation (LPO) in the hypothalamic and prefrontal regions of the brain by spectrophotometric method. It was found that under the influence of melanocortins, there is a pronounced suppression of the processes of free radical oxidation in the hypothalamic and prefrontal regions of the brain, which arose against the background of a stressful load which is manifested by a decrease in the indicators of the oxidative process.

scholarly journals Biochemical evaluation of excisional wound healing in rats with diabetes mellitus after using different methods of wound closure

2017 ◽  
Author(s):  
Ye. O. Loza ◽  
M. I. Marushchak
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
Antioxidant Defense System ◽  
Male Rats ◽  
Defense System ◽  
Radical Oxidation ◽  
Oxidation Processes

Introduction. Wound healing according to modern concepts is a coordinated process that passes through certain stages with the participation of different cells and products of its vital functions that regulate the healing process. It is well known that in patients with diabetes there is a violation of the process of wound healing, although until now, all the features of diabetes underlying such an inclination have not been fully understood.The aim of the study – to identify the features of oxidative stress on the background of diabetes mellitus in the rat's homogenate when different methods of closing wounds had been used. Research Methods. The experiment was conducted on 60 male rats weighing 280–320 g, which pre-simulated diabetes mellitus and surgical wounds. To detect the activation of free radical oxidation processes, the content of active forms of oxygen (AFC), lipid hydroperoxides (HPL), diene conjugates (DK) and trienic conjugates (TC) and oxidation modifications of proteins (OMP370 and OMP430). To study the antioxidant defense system, activity of superoxide dismutase was determined. Also, the activity of catalase and sulfhydryl groups (SH-groups) was determined.Results and Discussion. The research conducted by us showed that during the wounded process, on the background of diabetes, the activity of free radical oxidation processes increased. However, in the animal skin homogenates, which are labeled "Dermabond", all indices are significantly lower than in the group of animals that have been sewed over the course of all research periods. In the early stages of scar formation (3 days), an increase in the activity of the antioxidant defense system in both groups was observed. At the same time, in the experimental group of animals, which imposed the nodal seams, the activity of SOD and catalase significantly exceeded the skin index of animals, which applied glue and made (199.81±7.59) UM and (175.02±8.31) cat/kg, respectively.Conclusion. The use of skin glue reduces the intensity of the course of free radical oxidation in the cells of the scar tissue of the postoperative wound in conditions of diabetes mellitus in comparison with the imposition of nodal sutures on it: in the skin of animals, which is applied to the "Dermabond" glue, all indicators are significantly lower than in the group of animals, which imposed seam joints, throughout all research periods. 

scholarly journals CHARACTERISTIC OF FREE-RADICAL DAMAGE AND ANTIOXIDANT PROTECTION STATE IN PATIENTS WITH CHRONIC KIDNEY DISEASE AGAINST A BACKGROUND OF MALABSORPTION SYNDROME

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
А.Е. Shkarutyak
Keyword(s):  
Chronic Kidney Disease ◽  
Free Radical ◽  
Kidney Disease ◽  
Free Radical Oxidation ◽  
Oxidation Products ◽  
Essential Factor ◽  
Free Radical Damage ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Radical Damage

Oxidation processes play an important role in damage of the renal structures, especiallyagainst a background of other serious pathologies, in particular, malabsorptionsyndrome.The study of the mechanisms of mutual aggravation and progression of kidneydamage against a background of malabsorption is a very urgent problem of the presenttime.Purpose - to investigate the state of free radical oxidation of lipids, proteins andantioxidant defense in patients with chronic kidney disease of different ages withoxaluria against a background of malabsorption syndrome.Material and methods. 98 patients with chronic kidney disease of the I-II stages(pyelonephritis) with the presence of oxaluria against a background of malabsorptionsyndrome of different age were examined.The age of the patients under examination was32 - 64. All patients underwent a study of the system of free radical oxidation of lipidsand proteins.Results. chronic kidney disease with the presence of oxaluria is accompanied by asignificant increase in the blood content of free radical oxidation products, namelyaldehyde and ketone dinitrophenylhydrazones, especially of a neutral nature,malondialdehyde, as well as a significant decrease in the indicators of the antioxidantdefense system, which manifested itself to a greater extent in patients with older age.groups.Conclusion. An essential factor in the development and progression of chronic kidneydisease with the presence of oxaluria against a background of malabsorption syndromeis an increase in the intensity of free radical oxidation of lipids and proteins (mainly dueto aldehyde and ketondinitrophenylhydrazones of a neutral nature).

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals Effects of His-Phe-Arg-Trp-Pro-Gly-Pro peptide on free-radical oxidation processes in conditions of chronic restraint stress

2021 ◽  
Author(s):  
AO Vorvul ◽  
II Bobyntsev ◽  
OA Medvedeva ◽  
IuE Azarova ◽  
AE Belykh ◽  
...  
Keyword(s):  
Free Radical ◽  
Restraint Stress ◽  
Free Radical Oxidation ◽  
Regulatory Peptides ◽  
Thiobarbituric Acid ◽  
Synthetic Analog ◽  
Chronic Restraint Stress ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Wide Range

Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. Currently, a wide range of peptide neurotropic drugs, affecting the stress response development, are used in medicine, and new promising molecules are being studied. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. Serum levels of 8-oxo-2'-deoxyguanosine (8-OHdG) and superoxide dismutase 3 (SOD3) were assessed by enzyme immunoassay, and the levels of thiobarbituric acid reactive substances (TBARS) were assessed by fluorimetric method. CRS lead to the significant increase in the 8-OHdG levels by 18.4% (p = 0.01) and the decrease in the SOD3 levels by 14.3% (p = 0.01), however, it had no effect on the levels of TBARS. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. Thus, it was found that CRS resulted in oxidative stress in animals. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes.

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