scholarly journals THE USE OF PLASMAPHERESIS IN TREATMENT OF PATIENTS WITH INFERTILITY, PERITONEAL ENDOMETRIOSIS AND NAT2 GENE POLYMORPHISM

2016 ◽  
Vol 2 ◽  
pp. 3-10 ◽  
Author(s):  
Ekaterina Dubinskaya ◽  
Natalia Lapteva ◽  
Yana Lukyanova
Keyword(s):  
Oxidative Stress ◽  
Point Mutations ◽  
Free Radical Oxidation ◽  
Blood Rheology ◽  
Comprehensive Treatment ◽  
Radical Oxidation ◽  
Treatment Techniques ◽  
Peritoneal Endometriosis ◽  
Nat2 Gene ◽  
And Oxidative Stress

It is known that 30–40 % of patients with peritoneal endometriosis suffer from infertility. Half of the patients with endometriosis are identified point mutation in NAT2 – gene, which plays an important role in the acetylation of aromatic and heterocyclic amines, in the accumulation of endotoxins, activation of free radical oxidation, impaired microcirculation. These factors involve the use of methods of gemapheresis which have detoxification, the blood rheology corrective and immune corrective effects. The purpose of this study was to evaluate the efficacy of therapeutic plasma exchange in treatment of patients with peritoneal form of endometriosis, infertility and point mutations in the gene NAT2. The study included 140 patients with infertility, peritoneal form of endometriosis and point mutations in the gene NAT2. All patients are performed laparoscopy, coagulation foci of endometriosis. In the following 93 (66.4 %) patients were treated with a the course of therapeutic plasmapheresis using the apparatus «PCS-2» with the removal of 20–25 % the volume of circulating plasma with replacement plasma of crystalloid and colloid solutions. Before treatment were shown the signs of endotoxemia, activation of oxidative stress. After treatment with the use of plasmapheresis was revealed the significant reduction of endogenous intoxication parameters and oxidative stress. Also is noted the increase in the pregnancy rate, both independently and in IVF programs, especially during the first 3 months after treatment. The findings suggest that the efficiency of the proposed comprehensive treatment techniques (laparoscopy and subsequent course of therapeutic plasmapheresis) of patients with peritoneal endometriosis and infertility and with point mutations in the gene NAT2. The use of plasmapheresis is pathogenetically justified in patients of the studied group.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

scholarly journals PSX-3 Antioxidant system characteristics in Saanen goats depending on performance

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 461-462
Author(s):  
Polina D Bokhan ◽  
Alesya A Bakhta ◽  
Larisa Y Karpenko ◽  
Anna I Kozitcyna ◽  
Anna B Balykina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Milk Yield ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Dairy Goat ◽  
Blood Levels ◽  
Lactation Performance ◽  
Radical Oxidation ◽  
System Characteristics

Abstract Dairy goat breeding is widespread worldwide. Goat milk and other derivative products are an important part of nutrition. Dairy products are the most important part of goat breeding; therefore, lactation performance is a relevant issue. The purpose of this study was to evaluate antioxidant system characteristics in Saanen goats depending on lactation performance. The experiment was conducted in the north-western region of Russian Federation, in the laboratories of the biochemistry and physiology departments at FSBEI of Higher Education «SPbSAVM». The three experimental groups included 30 Saanen goats each, 2nd–3rd lactations, selected using matched pairs method. 1st group included low milk producing ability goats (< 600 kg of milk yield per year), 2nd group included average milk producing ability goats (600–800 kg of milk yield per year), 3rd group included high milk producing ability goats (>800 kg of milk yield per year). The blood samples were taken once: at peak lactation performance (45 days after parturition). The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P < 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics, we found a high intensity of free-radical oxidation in high milk producing ability goats. This results in oxidative stress development. Activity of anti-oxidizing enzymes was elevated; therefore, free-radical oxidation is intensive. Consequently, these data allow us to take into consideration exogenous anti-oxidizing agents administration in high milk producing ability goats in order to reduce oxidative stress.

The influence of humus acids of peloids and its components on free radical processes

2018 ◽  
Vol 64 (5) ◽  
pp. 429-432 ◽  
Author(s):  
N.P. Avvakumova ◽  
F.H. Kamilov ◽  
A.V. Zhdanova ◽  
I.A. Men’shikova ◽  
Yu.V. Zhernov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Albino Rats ◽  
Redox Systems ◽  
Radical Oxidation ◽  
The Third ◽  
Glutathione Peroxidases ◽  
Humus Acids ◽  
Radical Processes

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.

PSXI-25 Antioxidant system characteristics in Saanen goats depending on age

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 486-487
Author(s):  
Semen S Mudruk ◽  
Alesya A Bakhta ◽  
Larisa Y Karpenko ◽  
Anna I Kozitcyna ◽  
Anna B Balykina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Blood Levels ◽  
Leningrad Region ◽  
Radical Oxidation ◽  
The North ◽  
Age Related ◽  
Student’S T ◽  
System Characteristics

Abstract Goat husbandry is a relevant and significant research topic. The goat age is possible to reach 15–18 years and it is important to study not only performance processes, but also to evaluate age-related changes. The purpose of this study was to evaluate antioxidant system characteristics in Saanen goats depending on age. The experiment was conducted in the north-western region of Russian Federation, the Leningrad Region. The five experimental groups included 30 Saanen goats each, selected using matched pairs method. 1st group included 1 year old goats, 2nd group included 2-year-old goats, 3rd group included 3-year-old goats, 4th group included 4-year-old goats and 5th group included 5-year-old animals. The blood samples were taken once –during the summer season. The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P < 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics there were revealed high intensity of free-radical oxidation in aged 4–5-year-old goats. This is explained by oxidative stress development. Noticeably that anti-oxidizing enzymes activity was reduced by the age of 5 years – the possible reason for lacking compensation of developed oxidative stress. By the age of 4 activity of anti-oxidizing enzymes was elevated marking the compensation stage of oxidative stress, this elevation is statistically significant. Consequently, these data allow taking into consideration exogenous anti-oxidizing agents administration in 5 years and older goats in order to reduce oxidative stress.

Evaluation of oxidative stress and antioxidant activity in women with moderate and severe preeclampsia in the third trimester of pregnancy and their newborns

2020 ◽  
Vol 65 (12) ◽  
pp. 733-737
Author(s):  
Irina Gennadievna Popova ◽  
O. G. Sitnikova ◽  
S. B. Nazarov ◽  
R. I. Sadov ◽  
I. A. Panova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Third Trimester ◽  
Radical Oxidation ◽  
The Third ◽  
Uncomplicated Pregnancy ◽  
Group 2 ◽  
Group 1

We examined 66 women who were 22-40 weeks pregnant and their newborns. Of these, 15 women with moderate PE were in group 1, 22 women with severe PE were in group 2, and 55 women with uncomplicated pregnancy without hypertensive disorders were in the control group. Blood was taken from women when they were admitted to the clinic, and newborns ‘ blood was taken for 3-5 days of life. Free radical oxidation and antioxidant activity were evaluated by induced chemiluminescence. It was found that in patients with severe and moderate preeclampsia, the development of oxidative stress is accompanied by a weakening of antioxidant activity. In newborns born to mothers with preeclampsia, oxidative stress is accompanied by a compensatory increase in antioxidant activity.

scholarly journals Melatonin maintains the function of the blood redox system at combined ethanol-induced toxicity and subclinical inflammation in mice

2020 ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko ◽  
Oleksandr Lukash ◽  
Pawel J. Winklewski ◽  
Magdalena Wszedybyl-Winklewska
Keyword(s):  
Oxidative Stress ◽  
Free Radical Oxidation ◽  
Redox System ◽  
Erythrocyte Membranes ◽  
Redox Systems ◽  
Negative Effects ◽  
Melatonin Treatment ◽  
Radical Oxidation ◽  
Acute Ethanol ◽  
High Level

Abstract Background The goal of this study was to assess the effect of melatonin on blood redox systems in mice simultaneously exposed to ethanol and low-dose lipopolysaccharide (LPS). Methods Oxidative stress parameters were assessed in eight groups: untreated control, melatonin (10 mg kg−1, 10 days), LPS (injected once intraperitoneally at a dose of 150 μg per mouse), LPS with previous melatonin treatment, acute ethanol-induced stress (AES, 0.75 g kg−1 per day, 10 days), AES with previous melatonin treatment, LPS- and AES-induced toxicity, and melatonin treatment. Results Both ethanol and LPS induced oxidative stress. The combination of these two factors was even more toxic to the organism. Melatonin stabilized erythrocyte membranes and decreased the high level of free radical oxidation at the initial and final stages. Furthermore, melatonin limited protein damage through maintenance in the functional ability of the blood redox system to counteract pathological conditions. Conclusions Melatonin limited the negative effects associated with alcohol consumption and low-intensity inflammation.

scholarly journals Quercetin improves myocardial redox status in rats with type 2 diabetes

2021 ◽  
Vol 55 (3) ◽  
pp. 142-152
Author(s):  
Nataliia I. Gorbenko ◽  
Oleksii Yu. Borikov ◽  
Tetiana V. Kiprych ◽  
Olha V. Ivanova ◽  
Kateryna V. Taran ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Free Radical Oxidation ◽  
Redox Status ◽  
Diabetic Rats ◽  
The Body ◽  
Dependent Manner ◽  
Citrate Buffer ◽  
Radical Oxidation

Abstract Objective. Emerging data indicate that oxidative stress is closely associated with the pathogenesis of cardiovascular disease in type 2 diabetes mellitus (T2DM). The present study aimed to assess the effect of the most abundant flavonoid in the human diet quercetin (Q) on the myocardial redox status in rats with T2DM. Methods. T2DM was induced in male Wistar rats by a high caloric diet (for 14 weeks) and two streptozotocin (25 mg/kg b.w.) injections applied in four weeks of the diet, once a week for two weeks. The Q was administered intragastrically by gavage in a dose of 10 or 50 mg/kg of the body weight for 8 weeks starting from the 8th day after the last streptozotocin injection. The control rats received citrate buffer and seven days after the last STZ injection, basal glucose levels were measured in all animals. Results. Administration of Q increased insulin sensitivity in diabetic rats with more pronounced effect at a dose of 50 mg/kg b.w. The Q also decreased free radical oxidation in the heart mitochondria of diabetic animals, thus limiting the formation of advanced oxidation protein products in a dose-dependent manner and normalized the activity of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, glutathione reductase) in cardiac mitochondria independently of the dose used. In addition, the Q in both doses prevented the development of oxidative stress in the T2DM rats cardiomyocytes by reducing NADPH oxidase and xanthine oxidase activities. Conclusions. The findings demonstrate that Q in both doses 10 mg/kg and 50 mg/kg can protect from the development of oxidative stress in cardiomyocytes in the diabetic rats. The present data indicate that the use of Q may contribute to the amelioration of cardiovascular risk in patients with T2DM.

scholarly journals Free Radical Oxidation and Sleep Disorders in Andro- and Menopause (Literature Review)

2020 ◽  
Vol 5 (1) ◽  
pp. 31-41
Author(s):  
N. V. Semenova ◽  
I. M. Madaeva ◽  
L. I. Kolesnikova
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Sleep Deprivation ◽  
Sleep Disorders ◽  
Sleep Apnea Syndrome ◽  
Free Radical Oxidation ◽  
Carbonyl Stress ◽  
Apnea Hypopnea Index ◽  
Radical Oxidation ◽  
Obstructive Sleep

This review presents data on changes in the physiology of sleep during reproductive aging. It is noted that insomnia and obstructive sleep apnea syndrome (OSAS) are the main sleep disorders. The results of foreign and domestic studies in the field of free radical oxidation during sleep deprivation in animal models are presented, indicating the dependence of processes on the duration of sleep deprivation. The largest number of studies of free radical processes in a person with somnological pathology was carried out in the study of OSAS. Blood, urine, saliva, condensate of exhaled air can be biomaterial for determining the parameters of free radical oxidation. It was shown that the intensity of oxidative stress depends on the severity of OSAS, as evidenced by the positive correlation of the level of active products of thiobarbituric acid, the products of oxidation of proteins and carbonyl groups with the apnea/hypopnea index, determining the development of not only oxidative, but also carbonyl stress in patients with a severe degree OSAS. Biomarkers such as thioredoxin, malondialdehyde, superoxide dismutase, and reduced iron have shown a more stable relationship between increased oxidative stress and OSA. Despite the results obtained, the question of the association of oxidative stress and hypoxia in OSA remains debatable, which is associated with the opposite results of some studies. Insomnia, which occurs mainly in females, is accompanied by a high level of end products of lipid peroxidation with a decrease in the activity of antioxidants such as paraoxonase, an enzymatic component of the glutathione system. Along with this, menopausal women present low levels of uric acid, which correlates with high scores of the Pittsburgh sleep quality index questionnaire. Recent studies have identified an association between the activity of the «lipoperoxidation – antioxidants» system and the Clock 3111T/C gene polymorphism in menopausal Caucasian women, indicating the protective role of the minor allele.

scholarly journals Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Elena Menshchikova ◽  
Nikolay Zenkov ◽  
Victor Tkachev ◽  
Oksana Potapova ◽  
Liliya Cherdantseva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Granulomatous Inflammation ◽  
Free Radical Oxidation ◽  
Peritoneal Exudate ◽  
Numerical Density ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Bcg Granulomatosis ◽  
Tuberculous Granuloma

Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice.Methods. Chronic granulomatous inflammation was induced in male BALB/c mice by intravenously injecting the BCG vaccine, and the production of oxidative stress (activity of free-radical oxidation processes) and histological changes in the lungs, liver, and peritoneal exudate were measured 3, 30, 60, and 90 days after infection.Results. The tuberculous granuloma numerical density and diameter continuously increased from day 30 to day 90, and the macrophage content within the granulomas progressively diminished with a concomitant elevation in the number of epithelioid cells. The activity of the free-radical oxidation processes in the liver (i.e., the intensity of the homogenate chemiluminescence) reached a maximum at postinfection day 60 and subsequently began to decrease. The peak generation of reactive oxygen species by phagocytes in the peritoneal exudate (measured using flow cytometry) was also shifted in time and fell on day 30.Conclusions. The rise in the steady-state concentration of H2O2in the liver of mice with BCG-induced granulomatosis is not related to local H2O2production by phagocytes, and a decrease in the severity of generalized inflammation precedes the resolution of local inflammation.

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