scholarly journals Effects of His-Phe-Arg-Trp-Pro-Gly-Pro peptide on free-radical oxidation processes in conditions of chronic restraint stress

2021 ◽  
Author(s):  
AO Vorvul ◽  
II Bobyntsev ◽  
OA Medvedeva ◽  
IuE Azarova ◽  
AE Belykh ◽  
...  
Keyword(s):  
Free Radical ◽  
Restraint Stress ◽  
Free Radical Oxidation ◽  
Regulatory Peptides ◽  
Thiobarbituric Acid ◽  
Synthetic Analog ◽  
Chronic Restraint Stress ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Wide Range

Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. Currently, a wide range of peptide neurotropic drugs, affecting the stress response development, are used in medicine, and new promising molecules are being studied. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. Serum levels of 8-oxo-2'-deoxyguanosine (8-OHdG) and superoxide dismutase 3 (SOD3) were assessed by enzyme immunoassay, and the levels of thiobarbituric acid reactive substances (TBARS) were assessed by fluorimetric method. CRS lead to the significant increase in the 8-OHdG levels by 18.4% (p = 0.01) and the decrease in the SOD3 levels by 14.3% (p = 0.01), however, it had no effect on the levels of TBARS. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. Thus, it was found that CRS resulted in oxidative stress in animals. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes.

scholarly journals Influence of neuropeptides acth(4-7)-pro-gly-pro and acth(6-9)-pro-gly-pro on the intensity of redox reactions under experimental depression

2020 ◽  
Vol 10 (3) ◽  
pp. 29-32
Author(s):  
Marina Samotrueva ◽  
Anna Yasenyavskaya ◽  
Aleksandra Tsibizova ◽  
Jumazia Erizhepova ◽  
Nikolai Myasoedov ◽  
...  
Keyword(s):  
Free Radical ◽  
Social Stress ◽  
Redox Reactions ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Initial Content ◽  
The Brain

The experiment is devoted to the study of the antioxidant properties of neuropeptides from melanocortins ACTH(4-7)-Pro-Gly-Pro (Semax) and ACTH(6-9)-ProGly-Pro under conditions of experimental depression. The study was carried out on white outbred male rats. In the process of modeling experimental depression (social stress) inter-male confrontations were observed as a result of which groups of animals with aggressive and submissive behaviors were formed. The free radical oxidation processes were assessed by determining the activity of catalase, the initial content of malondialdehyde (MDA), the rate of spontaneous and ascorbate-dependent lipid peroxidation (LPO) in the hypothalamic and prefrontal regions of the brain by spectrophotometric method. It was found that under the influence of melanocortins, there is a pronounced suppression of the processes of free radical oxidation in the hypothalamic and prefrontal regions of the brain, which arose against the background of a stressful load which is manifested by a decrease in the indicators of the oxidative process.

scholarly journals CHARACTERISTIC OF FREE-RADICAL DAMAGE AND ANTIOXIDANT PROTECTION STATE IN PATIENTS WITH CHRONIC KIDNEY DISEASE AGAINST A BACKGROUND OF MALABSORPTION SYNDROME

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
А.Е. Shkarutyak
Keyword(s):  
Chronic Kidney Disease ◽  
Free Radical ◽  
Kidney Disease ◽  
Free Radical Oxidation ◽  
Oxidation Products ◽  
Essential Factor ◽  
Free Radical Damage ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Radical Damage

Oxidation processes play an important role in damage of the renal structures, especiallyagainst a background of other serious pathologies, in particular, malabsorptionsyndrome.The study of the mechanisms of mutual aggravation and progression of kidneydamage against a background of malabsorption is a very urgent problem of the presenttime.Purpose - to investigate the state of free radical oxidation of lipids, proteins andantioxidant defense in patients with chronic kidney disease of different ages withoxaluria against a background of malabsorption syndrome.Material and methods. 98 patients with chronic kidney disease of the I-II stages(pyelonephritis) with the presence of oxaluria against a background of malabsorptionsyndrome of different age were examined.The age of the patients under examination was32 - 64. All patients underwent a study of the system of free radical oxidation of lipidsand proteins.Results. chronic kidney disease with the presence of oxaluria is accompanied by asignificant increase in the blood content of free radical oxidation products, namelyaldehyde and ketone dinitrophenylhydrazones, especially of a neutral nature,malondialdehyde, as well as a significant decrease in the indicators of the antioxidantdefense system, which manifested itself to a greater extent in patients with older age.groups.Conclusion. An essential factor in the development and progression of chronic kidneydisease with the presence of oxaluria against a background of malabsorption syndromeis an increase in the intensity of free radical oxidation of lipids and proteins (mainly dueto aldehyde and ketondinitrophenylhydrazones of a neutral nature).

scholarly journals Biochemical evaluation of excisional wound healing in rats with diabetes mellitus after using different methods of wound closure

2017 ◽  
Author(s):  
Ye. O. Loza ◽  
M. I. Marushchak
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
Antioxidant Defense System ◽  
Male Rats ◽  
Defense System ◽  
Radical Oxidation ◽  
Oxidation Processes

Introduction. Wound healing according to modern concepts is a coordinated process that passes through certain stages with the participation of different cells and products of its vital functions that regulate the healing process. It is well known that in patients with diabetes there is a violation of the process of wound healing, although until now, all the features of diabetes underlying such an inclination have not been fully understood.The aim of the study – to identify the features of oxidative stress on the background of diabetes mellitus in the rat's homogenate when different methods of closing wounds had been used. Research Methods. The experiment was conducted on 60 male rats weighing 280–320 g, which pre-simulated diabetes mellitus and surgical wounds. To detect the activation of free radical oxidation processes, the content of active forms of oxygen (AFC), lipid hydroperoxides (HPL), diene conjugates (DK) and trienic conjugates (TC) and oxidation modifications of proteins (OMP370 and OMP430). To study the antioxidant defense system, activity of superoxide dismutase was determined. Also, the activity of catalase and sulfhydryl groups (SH-groups) was determined.Results and Discussion. The research conducted by us showed that during the wounded process, on the background of diabetes, the activity of free radical oxidation processes increased. However, in the animal skin homogenates, which are labeled "Dermabond", all indices are significantly lower than in the group of animals that have been sewed over the course of all research periods. In the early stages of scar formation (3 days), an increase in the activity of the antioxidant defense system in both groups was observed. At the same time, in the experimental group of animals, which imposed the nodal seams, the activity of SOD and catalase significantly exceeded the skin index of animals, which applied glue and made (199.81±7.59) UM and (175.02±8.31) cat/kg, respectively.Conclusion. The use of skin glue reduces the intensity of the course of free radical oxidation in the cells of the scar tissue of the postoperative wound in conditions of diabetes mellitus in comparison with the imposition of nodal sutures on it: in the skin of animals, which is applied to the "Dermabond" glue, all indicators are significantly lower than in the group of animals, which imposed seam joints, throughout all research periods. 

scholarly journals Evaluation of selenium status and effectiveness of selenolin in the treatment of selenium-dependent hepatopathies

2021 ◽  
Vol 262 ◽  
pp. 02004
Author(s):  
Vladimir A. Grin ◽  
Eugenia V. Rogaleva ◽  
Marina P. Semenenko ◽  
Ksenia A. Semenenko ◽  
Elena V. Kuzminova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Liver Diseases ◽  
Clinical Status ◽  
Clinical Signs ◽  
Free Radical Oxidation ◽  
Selenium Status ◽  
Radical Oxidation ◽  
Experimental Animals ◽  
Oxidation Processes

The article is devoted to the study of the assessment of selenium status in the pathogenesis of liver diseases and the effectiveness of the use of a selenium-containing drug. In the course of studies in animals with clinical signs and high rates of hepatopathies markers – transaminases, in 100% of cases a low level of selenium was revealed – 0.026 ±0.01 μg / ml, because of the increased consumption of this microelement on the background of increased free radical oxidation processes in the liver tissue. It was found that intramuscular administration of the drug selenolin at a dose of 20 mg / 100 kg four times with an interval of 15 days significantly improved the clinical status of the experimental animals; the effectiveness of therapy was 93.3%. Parenteral administration of selenolin favorably corrected the processes of lipid peroxidation and contributed to a significant (p <0.01) decrease in the level of liver indicator enzymes in comparison with the control by 34.5% (aspartate aminotransferase) and by 45.8% (alanine aminotransferase), and also contributed to an increase in the level of selenium to the level of the physiological norm (0.068 ± 0.03 μg / ml).

scholarly journals Assessment of the body’s nonspecific defense in workers exposed to noxious factors of chemical and petrochemical industry

2016 ◽  
Vol 97 (5) ◽  
pp. 784-792 ◽  
Author(s):  
R I Sabitova ◽  
E D Kravets ◽  
E F Galiullina ◽  
D F Shakirov ◽  
F Kh Kamilov ◽  
...  
Keyword(s):  
Free Radical ◽  
Energy Metabolism ◽  
Petrochemical Industry ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
The Body ◽  
Experimental Plant ◽  
Electrolyte Metabolism ◽  
Radical Oxidation ◽  
Oxidation Processes

Aim. To study the processes of free-radical oxidation, antioxidant defense, energy metabolism, electrolyte metabolism, and cytokine system reflecting the state of the non-specific defense of the body in workers exposed to complex of toxic substances under the conditions of manufacturing, substantiating the importance of indicators of preclinical diagnosis of disease development risk.Methods. The study included 90 workers of JSC «Experimental Plant Neftekhim», 95 workers of JSC «Kaustic» and 101 workers of JSC «Ufa plant of elastomeric materials, products and structures». Materials for the study were blood, mixed saliva, gingival fluid and urine. The laboratory studies were performed to evaluate the free radical oxidation processes, antioxidant defense, energy metabolism, electrolyte metabolism, and cytokine system. Taking into account the features of industrial factors, particularly their effects on the workers bodies, routes of toxic compounds entry by inhalation, oral cavity and skin of the hands, three professional groups were formed (A, B, C). Group A included employees having constant contact with chlororganic compounds. The group B included persons who have constant contact with the higher and lower aromatic hydrocarbons. A group C consisted of employees who have constant contact with a mixture of chemicals: rubber compound containing carcinogens - benzo(a)pyrene, NDMA, nitrosodiethylamine; white carbon black, rubber dust, talc, amine compounds, sulfur dioxide, carbon monoxide. The control group consisted of employees of administrative and managerial staff.Results. The study revealed that one of the major pathogenetic mechanisms of action of chemical and petrochemical industry hazards is activation of free radical oxidation.Conclusion. Among the mechanisms of the influence of chemical contaminants of working environment a leading role play intensification of free radical oxidation processes, failure and/or inhibition of the antioxidant defense components.

Assessment of functional state of cardio-vascular system and free radical oxidation processes in young athletes

10.12737/5021 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Юмали ◽  
S. Yumali ◽  
Фархутдинов ◽  
R. Farkhutdinov ◽  
Яковлева ◽  
...  
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Cardiovascular System ◽  
Functional State ◽  
Free Radical Oxidation ◽  
Antioxidant Effect ◽  
Stress Cardiomyopathy ◽  
Young Athletes ◽  
Radical Oxidation ◽  
Oxidation Processes

Oxidative stress underlies the formation of the pathology of cardiovascular system in young athletes. Its potential role in the pathogenesis of myocardial remodeling in young athletes has been discussed. The paper is devoted to the study of the changes of free radical oxidation in the body by the method of chemiluminescence and antioxidant effect on the functional state of the cardiovascular system. The results of clinical studies on the antioxidant effect of polydihidroxyphenilenthiosulfonate sodium («Hypoxen») in vitro, namely the addition of a substance in a simple model systems that mimic the most common reactions of free radical oxidation in the organism, such as formation of reactive oxygen species and lipid peroxidation. It has been found in this study that concentration of free radicals in the blood in athletes with physiological manifestations of athlete´s heart and signs of stress cardiomyopathy has been increased. After administration of Hypoxen in vivo the concentration of free radicals in the athlete’s blood has been found to be reduced. In assessing the functional state of the cardiovascular system administration of Hypoxen resulted in the improved physical performance in young athletes. Maintaining of free radical oxidation processes at the optimal levels contributes to timely prevention of stress cardiomyopathy.

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