Indexes of Lipid Metabolism in Fish from the Zaporizke Reservoir

The total lipid contents and iodine value of fats had been determined in fish from two sections of the Zaporizke Reservoir (Ukraine) with different contamination levels. Research was conducted using the muscle and liver tissue samples from pike-perch (Sander lucioperca), european perch (Perca fluviatis), prussian carp (Carassius gibelio), roach (Rutilus rutilus) and rudd (Scardinius erythrophthalmus). Obtained data showed that at the contaminated zone, the total lipid contents were significantly (p ≤ 0.05) reduced in muscle tissue of pike-perch and european perch in comparison with the samples from “conventionally clean” lower section of the reservoir. Increased iodine value of fats in muscle tissue and liver tissue were detected in the predatory fish and both fish groups respectively. The research results could be used for estimation of the adaptation processes in freshwater fish as well as for indication of environmental contamination level in the natural and artificial reservoirs.

Download Full-text

CHARACTERISTICS OF FREE-RADICAL PROCESSES IN THE ORGANISMS OF SOME PREDATORY FISH SPECIES IN DIFFERENT SEASONS OF THE YEAR

VESTNIK OF ASTRAKHAN STATE TECHNICAL UNIVERSITY SERIES FISHING INDUSTRY ◽

10.24143/2073-5529-2017-1-117-123 ◽

2017 ◽

pp. 117-123

Author(s):

Nikolay Aleksandrovich Pudovkin ◽

Peter Vladimirovich Smutnev

Keyword(s):

Fish Species ◽

Catalase Activity ◽

Perca Fluviatilis ◽

Selenium Concentration ◽

The Body ◽

Predatory Fish ◽

Volga River ◽

Silurus Glanis ◽

Average Value ◽

Pike Perch

The authors of the article have studied the content of the elements of anti-oxidant system (malondialdehyde, catalase, selenium) in tissues of the internal organs (gills, intestine, muscles, liver, swimbladder, scales) in some species of predatory fish (pike Esox lucius (L., 1758), perch Perca fluviatilis (L., 1758), pike-perch Sander lucioperca (L., 1758), catfish Silurus glanis ) widespread in the basin of the Volga river in the Saratov region. The lowest concentration of malondialdehyde in organisms of the studied fish species is observed in fall and winter; the highest - in spring and summer. Catalase activity in gills tissue of a pike raised in 11.8%, cat-fish - 9.1%, pike-perch - 7.5%, perch - 7.8%. In fall (compared to winter) enzyme activity lowering in gonads of pike-perch makes 16.3%, in gonads of perch - 14.4%. In other tissues there were not observed any evident changes of catalase activity. Fish species under consideration are listed according to the average value of selenium concentration in organisms, µg/g: pike (0.208) > catfish (0.207) > pike-perch (0.196) > perch (0.178). According to the average value of the selenium accumulation in the body in different season all the studied species can be placed in the following order, µg/g: winter-pike (0.132) > pike-perch (0.136) > perch and catfish (0.142); spring - pike-perch (0.190) > perch (0.191) > pike (0.208) > catfish (0.209); summer - perch (0.186) > pike-perch (0.190) > catfish and pike (0.203); autumn - perch (0.193) > pike-perch (0.268) > > catfish (0.274) > pike (0.289).

Download Full-text

Free amino acid content in muscle tissue of bighead carp and wels catfish

Medycyna Weterynaryjna ◽

10.21521/mw.5571 ◽

2016 ◽

Vol 72 (10) ◽

pp. 632-636 ◽

Cited By ~ 2

Author(s):

Renata Pyz-Łukasik ◽

Maria Szpetnar ◽

Waldemar Paszkiewicz ◽

Marcin R. Tatara ◽

Adam Brodzki

Keyword(s):

Amino Acid ◽

Free Amino Acid ◽

Muscle Tissue ◽

Acid Content ◽

Free Amino ◽

Amino Acid Content ◽

Bighead Carp ◽

Predatory Fish ◽

Free Amino Acid Content ◽

Natural Diet

The present research was aimed at a comparative analysis of free amino acid (FAA) levels in the muscle tissue of herbivorous and predatory fish. The FAA concentration in the muscle tissue samples from bighead carp and wels catfish was determined by ion-exchange chromatography. The bighead carp muscle tissue, as compared to that of wels catfish, showed significantly higher concentrations of arginine, histidine, methionine, phenyloalanine, alanine, asparagine, serine, glycine, and taurine, but significantly lower levels of isoleucine, leucine, threonine, valine, glutamine, cystationine, β-alanine, ethanoloamine, as well as aspartic, glutamic, cysteic, α- and γ-aminobutyric acids. The muscle tissues of bighead carp and wels catfish did not differ significantly in the levels of cystine, lysine, tryptophan, tyrosine, α-aminoadipic acid, cytrulline, ornithine and 1-methyl-histidine. Proline was detected only in the wels catfish muscle. The results obtained have shown differences in free amino acid concentration in muscle tissue of examined fish. A differentiated natural diet induces changes in free amino acid content in fish tissues. Knowledge of levels of free amino acids which are precursors for biogenic amines facilitates setting the safety criteria for fish and fishery products from species other than those mentioned in the Commission Regulation No 2073/2005

Download Full-text

Lipids of the fin whale (Balaenoptera physalus) from North Atlantic waters. IV. Fin whale milk

Canadian Journal of Biochemistry ◽

10.1139/o68-029 ◽

1968 ◽

Vol 46 (3) ◽

pp. 197-203 ◽

Cited By ~ 14

Author(s):

R. G. Ackman ◽

C. A. Eaton ◽

S. N. Hooper

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Total Lipid ◽

Iodine Value ◽

North Atlantic ◽

Methyl Esters ◽

Short Chain Fatty Acids ◽

Fin Whale ◽

Hydrolysis Of

Fatty acid compositions were determined for total lipid (17.5% of the milk and > 95% triglycerides), 2-monoglyceride obtained by enzymatic hydrolysis of isolated triglyceride, and isolated phospholipid (~1% of total lipids). The total lipid fatty acids of the milk had a composition similar to fin whale depot fat but were enriched in hexadecanoic acid and polyunsaturated fatty acids at the expense of monoethylenic acids; correspondingly the iodine value of 136 (methyl esters) was higher than the normal range (105–120) of North Atlantic fin whale blubber oils. Over 80% of the fatty acids in the 2-position of the triglycerides were accounted for by relatively short chain fatty acids, especially hexadecanoic (54.6%), tetradecanoic (13.7%), and hexadecenoic (11.2%), so that the ester iodine value was only 48. The milk phospholipids had a fatty acid composition basically similar to that of liver phospholipids (methyl ester iodine value 120) with somewhat more polyunsaturated fatty acids and accordingly an iodine value of 144 for methyl esters.

Download Full-text

Development of a Method to Determine Albendazole and Its Metabolites in the Muscle Tissue of Yellow Perch Using High-Performance Liquid Chromatography with Fluorescence Detection

Journal of AOAC International ◽

10.1093/jaoac/94.2.446 ◽

2011 ◽

Vol 94 (2) ◽

pp. 446-452 ◽

Cited By ~ 4

Author(s):

Donglei Yu ◽

Nathan Rummel ◽

Badar Shaikh

Keyword(s):

High Performance Liquid Chromatography ◽

Muscle Tissue ◽

Mobile Phase ◽

Yellow Perch ◽

High Performance ◽

Ammonium Acetate ◽

Hplc Method ◽

Tissue Samples ◽

Phase Combination

Abstract An HPLC method was developed for the determination of albendazole (ABZ) and its metabolites, a sulfoxide (ABZSO), a sulfone (ABZSO2), and albendazole-2-aminosulfone (ABZ-2-NH2SO2), from yellow perch muscle tissue with adhering skin. The muscle tissue samples were made alkaline with potassium carbonate and extracted with ethyl acetate, followed by a series of liquidliquid extraction steps. After solvent evaporation, the residue was reconstituted in the initial mobile phase combination of the gradient. The mobile phase consisted of a buffer, 50 mM ammonium acetate (pH 4.0) in 10 methanolwater, and 100 acetonitrile. The gradient was from 20 acetonitrile to 85 acetonitrile. The analytes were chromatographed on an RP Luna C18(2) column and detected by fluorescence with excitation and emission wavelengths of 290 and 330 nm, respectively. The average recoveries from fortified muscle tissue for ABZ (20100 ppb), ABZ-SO (20200 ppb), ABZSO2 (8100 ppb), and ABZ-2-NH2SO2 (20100 ppb) were 85, 95, 101, and 86, respectively, with corresponding CV values of 9, 3, 6, and 4, respectively. Their LOQ values were 10, 10, 1, and 10 ppb, respectively. The procedure was applied to determine ABZ and its major metabolites in the incurred muscle tissue of yellow perch obtained after orally dosing the fish with ABZ.

Download Full-text

Quantitation and Confirmation of Sulfamethazine Residues in Swine Muscle and Liver by LC and GC/MS

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/74.3.479 ◽

1991 ◽

Vol 74 (3) ◽

pp. 479-482 ◽

Cited By ~ 1

Author(s):

Germain Carignan ◽

Karen Carrier

Keyword(s):

Muscle Tissue ◽

Liver Tissue ◽

Internal Standard ◽

Mass Spectrometric ◽

Selected Ion Monitoring ◽

Tissue Extracts ◽

Coefficients Of Variation ◽

Swine Tissue ◽

Liquid Chromatographic ◽

Chromatographic Mass

Abstract The present paper describes a liquid chromatographic (LC) method for purification of crude swine tissue extracts before gas chromatographic/mass spectrometric (GC/MS) quantitation and confirmation of sulfamethazine at low ppb levels. Fractions corresponding to sulfamethazine were collected, evaporated to dryness, ^methylated with diazomethane, concentrated, and analyzed by GC/MS. A mass spectrometer was set to selected ion monitoring (SIM) mode. Ions 233, 227, 228, and 92 m/z were detected. Ratio 227/233 m/z (sulfamethazine/internal standard, [pheny| 13C6] sulfamethazine) was used for quantitation, while ratios 228/227 and 92/ 227 m/z, respectively, were used for confirmation. Quantitation in spiked blank muscle tissue was tested from 100 to 1 ppb and found acceptable at all concentrations studied; coefficients of variation ranged from 4.9 to 14.4%. Similar results were obtained for liver tissue from 5 to 20 ppb; coefficients of variation ranged from 1.2 to 9.1 %.

Download Full-text

Electrorotation of single microalgae cells during lipid accumulation for assessing cellular dielectric properties and total lipid contents

Biosensors and Bioelectronics ◽

10.1016/j.bios.2020.112772 ◽

2021 ◽

Vol 173 ◽

pp. 112772

Author(s):

Yu-Sheng Lin ◽

Sung Tsang ◽

Sakina Bensalem ◽

Ching-Chu Tsai ◽

Shiang-Jiuun Chen ◽

...

Keyword(s):

Dielectric Properties ◽

Total Lipid ◽

Lipid Accumulation ◽

Lipid Contents

Download Full-text

Hepatic Metabolomic Analysis in Mice Fed a High Fat Diet with Watermelon and Watermelon Byproducts Shows Improved Lipid Metabolism and Reduction of Chronic Inflammation (P06-023-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz031.p06-023-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Alexandra Becraft ◽

Marlena Sturm ◽

Gavin Pierce ◽

Rufa Mendez ◽

Neil Shay

Keyword(s):

Chronic Inflammation ◽

High Fat Diet ◽

Liver Tissue ◽

Value Added ◽

Principal Component ◽

Metabolomic Analysis ◽

High Fat ◽

Tissue Samples ◽

Metabolic Conditions ◽

Ultrahigh Performance Liquid Chromatography

Abstract Objectives Watermelon is a nutrient-dense fruit shown previously to produce health benefits, particularly regarding blood pressure regulation. We tested the hypothesis that intake of whole watermelon flesh and value-added watermelon components would improve metabolic conditions in C57BL/6 J male mice fed a high-fat, high-sucrose diet modeling an obesogenic Western diet (HF). We further hypothesize that metabolomic profiling will show changes in relative levels of compounds related to lipid and glucose metabolism, and chronic inflammation. Methods In a prior study (Becraft et al., 2018), groups of mice (n = 8) were provided either low-fat diet (LF, 10% kcal fat), high-fat diet (HF, 45% kcal fat), HF plus Watermelon Skin (HF + WS), HF plus Watermelon Rind (HF + WR), or HF plus Watermelon Flesh (HF + WF) for 10 weeks. Watermelon flesh was provided at 10% of total energy and skin and rind were added at ∼ 0.2% (w/w) of diet. After ten weeks, animals were euthanized, and liver tissue saved for metabolomic analysis. Liver tissue samples were homogenized, and an identical mass equivalent of liver was subjected to methanol extraction and split into aliquots for analysis by ultrahigh performance liquid chromatography/mass spectrometry in the positive, negative or polar ion mode. There were 709 biochemicals identified and analyzed between groups. Welch's 2-sample t-test was performed with ArrayStudio (Omicsoft) or R software on log transformed data to compare data between experimental groups. Estimate of the false discovery rate (Q value) was calculated and Q ;< 0.05 used as an indication of high confidence in a result. Results Principal component analysis showed segregation of groups along three different components, representing 24.8%, 19.4%, and 9.0% of the variation. Profound differences were found in LF vs. HF liver tissue. Compared to HF-fed mice, mice fed WF showed reduced levels of bile acids and pro-inflammatory compounds 12-HETE, 15, HETE, and PGF2 (all P < 0.05) in the liver. Conclusions In mice consuming a high-fat western style diet, regular intake of watermelon flesh, and fiber-rich products made from rind and skin all improved metabolism as evidenced by metabolomic analysis of liver tissue. Most notably were reductions in pro-inflammatory compounds including HETEs and Prostaglandin F2. Funding Sources National Watermelon Promotion Board.

Download Full-text