scholarly journals The development of cell-adhesive hydrogel for 3D printing

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Kenichi Arai ◽  
Yoshinari Tsukamoto ◽  
Hirotoshi Yoshida ◽  
Hidetoshi Sanae ◽  
Tanveer Ahmad Mir ◽  
...  
Keyword(s):  
Enzymatic Reaction ◽  
Living Cells ◽  
Layer By Layer ◽  
Mixed Solution ◽  
Fibroblast Proliferation ◽  
Alginate Hydrogel ◽  
Adhesive Properties ◽  
Custom Made ◽  
Cell Adhesive ◽  
H2o2 Solution

Biofabrication has gained tremendous attention for manufacturing functional organs or tissues. To fabricate functional organs or tissues, it is necessary to reproduce tissue-specific micro to macro structures. Previously, we de-veloped a custom-made 3D-bioprinter with the capability to print and fabricate 3D complicated hydrogel structures composed of living cells. Through the gelation reaction, fine and complicated 3D gel structures can be fabricated via layer by layer printing. Alginate hydrogel has been used mainly due to its good fabricating properties. However, it is not a reliable platform for tissue regeneration because of its inadequate cell-adhesiveness. Therefore, our laboratory is in-terested to explore more suitable hydrogels for bioprinting and 3D tissue fabrication. In this study, we tried to fabricate 3D gel structures with enough cell-adhesive properties. We focused on hydrogel formation through enzymatic reaction by incorporating materials bearing phenolic hydroxyl moieties and horseradish peroxidase. We examined Alg-Ph and Alg-Ph/Gelatin-Ph gels. We used a mixed solution of applied materials as bioink and printed into H2O2 solution. We successfully fabricated the 3D gel sheet structures including fibroblasts cultures. Fibroblast proliferation and viability were also observed in the 3D gel sheet for more than one week. In conclusion, the hydrogel obtained through enzymatic reaction is a biocompatible bioink material which can be applied to fabricate 3D cell-adhesive gel structures using a 3D-bioprinter.

scholarly journals Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate

2017 ◽  
Vol 51 ◽  
pp. 279-293 ◽  
Author(s):  
Joana M. Silva ◽  
José R. García ◽  
Rui L. Reis ◽  
Andrés J. García ◽  
João F. Mano
Keyword(s):  
Layer By Layer ◽  
Adhesive Properties ◽  
Layer By Layer Assembly ◽  
Cell Adhesive ◽  
Layer Assembly

scholarly journals Transfer stamping of human mesenchymal stem cell patches using thermally expandable hydrogels with tunable cell-adhesive properties

Biomaterials ◽  
2015 ◽  
Vol 54 ◽  
pp. 44-54 ◽  
Author(s):  
Indong Jun ◽  
Yu Bin Lee ◽  
Yu Suk Choi ◽  
Adam J. Engler ◽  
Hansoo Park ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Adhesive Properties ◽  
Cell Adhesive

scholarly journals Β-Galactosidase-Catalyzed Fluorescent Reporter Labeling of Living Cells for Sensitive Detection of Cell Surface Antigens

2020 ◽  
Author(s):  
Katsuya Noguchi ◽  
Takashi Shimomura ◽  
Yuya Ohuchi ◽  
Munetaka Ishiyama ◽  
Masanobu Shiga ◽  
...  
Keyword(s):  
Cell Surface ◽  
Enzymatic Reaction ◽  
Wavelength Region ◽  
Living Cells ◽  
Fluorescent Dye ◽  
Surface Proteins ◽  
Quinone Methide ◽  
Fluorescence Signal ◽  
Cell Surface Antigens ◽  
Cell Surface Proteins

The ability to detect cell surface proteins using fluorescent dye-labeled antibodies is crucial for the reliable identification of many cell types. However, the different types of cell surface proteins used to identify cells are currently limited in number because they need to be expressed at high levels to exceed background cellular autofluorescence, especially in the shorter wavelength region. Herein, we report on a new method (CLAMP: quinone methide-based <u>c</u>atalyzed signa<u>l</u> <u>amp</u>lification) in which the fluorescence signal is amplified by an enzymatic reaction that strongly facilitates the detection of cell surface proteins on living cells. We used β-galactosidase as an amplification enzyme and designed a substrate for it, called MUGF, which contains a fluoromethyl group. Upon removal of the galactosyl group in MUGF by β-galactosidase labeling of the target cell surface proteins, the resulting quinone methide group-containing product was found to be both cell membrane permeable and reactive with intracellular nucleophiles, thereby providing fluorescent adducts. Using this method, we successfully detected several cell surface proteins including programmed death ligand 1 protein, which is difficult to detect using conventional fluorescent dye-labeled antibodies.

scholarly journals Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers

Micromachines ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 399 ◽  
Author(s):  
Shinako Bansai ◽  
Takashi Morikura ◽  
Hiroaki Onoe ◽  
Shogo Miyata
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Skeletal Muscles ◽  
Malignant Tumors ◽  
Three Dimensional ◽  
Living Cells ◽  
Cyclic Stretch ◽  
Core Shell ◽  
Custom Made ◽  
A Cell

Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.

scholarly journals Adsorption and Release of Rose Bengal on Layer-by-Layer Films of Poly(Vinyl Alcohol) and Poly(Amidoamine) Dendrimers Bearing 4-Carboxyphenylboronic Acid

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1854 ◽  
Author(s):  
Kentaro Yoshida ◽  
Akane Yamaguchi ◽  
Hiroki Midorikawa ◽  
Toshio Kamijo ◽  
Tetsuya Ono ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Boronic Acid ◽  
Vinyl Alcohol ◽  
Acidic Solution ◽  
Phenylboronic Acid ◽  
Poly Vinyl Alcohol ◽  
Basic Solution ◽  
Layer By Layer ◽  
Lbl Films ◽  
H2o2 Solution

Phenylboronic acid-bearing polyamidoamine dendrimer (PBA-PAMAM)/poly(vinyl alcohol) (PVA) multilayer films were prepared through the layer-by-layer (LbL) deposition of PBA-PAMAM solution and PVA solution. PBA-PAMAM/PVA films were constructed successfully through the formation of boronate ester bonds between the boronic acid moiety in PBA and 1,3-diol units in PVA. When the (PBA-PAMAM/PVA)5 films were immersed in rose bengal (RB) solution, RB was adsorbed onto the LbL films. The amount of RB adsorbed was higher in the LbL films immersed in acidic solution than in basic solution. The release of RB from the LbL films was also promoted in the basic solution, while it was suppressed in the acidic solution. The boronic acid ester is oxidized to phenol by hydrogen peroxide (H2O2) and the carbon-boron bond is cleaved, so that the (PBA-PAMAM/PVA)5 films can be decomposed by immersion in H2O2 solution. Therefore, when RB-adsorbed (PBA-PAMAM/PVA)5 films were immersed in H2O2 solution, the release of RB was moderately promoted when the solution was weakly acidic.

OS1101 Cell-adhesive properties on the biodegradable materials modified by atom beam

2008 ◽  
Vol 2008 (0) ◽  
pp. _OS1101-1_-_OS1101-2_
Author(s):  
Kumiko YOKOTA ◽  
Chiaki HIWA ◽  
Masahito TAGAWA ◽  
Taiji ADACHI ◽  
Yoshikazu NAKAI
Keyword(s):  
Biodegradable Materials ◽  
Atom Beam ◽  
Adhesive Properties ◽  
Cell Adhesive

scholarly journals Synthesis and Cell Adhesive Properties of Linear and Cyclic RGD Functionalized Polynorbornene Thin Films

2012 ◽  
Vol 13 (8) ◽  
pp. 2546-2553 ◽  
Author(s):  
Paresma R. Patel ◽  
Rosemary Conrad Kiser ◽  
Ying Y. Lu ◽  
Eileen Fong ◽  
Wilson C. Ho ◽  
...  
Keyword(s):  
Thin Films ◽  
Adhesive Properties ◽  
Cell Adhesive

Choline phosphate functionalized surface: protein-resistant but cell-adhesive zwitterionic surface potential for tissue engineering

2015 ◽  
Vol 51 (3) ◽  
pp. 487-490 ◽  
Author(s):  
Xingyu Chen ◽  
Tianchan Chen ◽  
Zaifu Lin ◽  
Xian'e Li ◽  
Wei Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Potential ◽  
Biomedical Applications ◽  
Surface Protein ◽  
Adhesive Properties ◽  
Cell Adhesive ◽  
Choline Phosphate ◽  
Protein Resistant ◽  
Functionalized Surface

A choline phosphate (CP) modified zwitterionic surface is prepared with both protein-resistant and cell-adhesive properties, exhibiting great potential for biomedical applications such as tissue engineering.

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