Effect of melatonin on antioxidant enzyme activities in blood erythrocytes of rats during acute emotional stress

The effect of the epiphyseal hormone melatonin on the activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), and Cu/Zn-superoxide dismutase (Cu/Zn-SOD) was studied in peripheral blood erythrocytes of behaviorally passive and active Wistar rats. Acute emotional stress was modeled by immobilization of animals for1 h with simultaneous electrocutaneous stimulation. Basal activity of antioxidant glutathione enzymes in erythrocytes of behaviorally passive rats was higher than that in active animals. Administration of melatonin (2 mg/kg, intraperitoneally) was accompanied by a decrease in the activity of GPx and GR in erythrocytes from non-stressed passive animals. After experimental stress, passive rats demonstrated a significant increase in the activity of Cu/Zn-SOD and GPx in peripheral blood erythrocytes. The absence of stress-induced changes in functional activity of antioxidant defense enzymes in the blood of behaviorally active animals suggests a relatively constant oxidative status of tissues in these animals under stress conditions. Melatonin administration had little effect on stress-induced changes in functional activity of the erythrocyte antioxidant system in passive rats. Active specimens pretreated with melatonin before stress exposure were characterized by activation of study antioxidant enzymes. Quantitative parameters of the erythrocyte antioxidant defense enzymes did not differ in behaviorally active and passive rats subjected to experimental stress after melatonin injection. Thus, exogenous melatonin abolishes differences in the activity of study antioxidant enzymes in erythrocytes of animals with different behavioral parameters under basal conditions and after experimental stress. In passive rats melatonin mainly reduced the initial tension of oxidative processes. By contrast, administration of this hormone to active specimens is followed by an increase in functional activity of the antioxidant enzyme system under conditions of acute stress.

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Genotypic dependent effect of exogenous glutathione on Cd-induced changes in proteins, ultrastructure and antioxidant defense enzymes in rice seedlings

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2011.06.011 ◽

2011 ◽

Vol 192 (3) ◽

pp. 1056-1066 ◽

Cited By ~ 49

Author(s):

Yue Cai ◽

Fangbin Cao ◽

Kang Wei ◽

Guoping Zhang ◽

Feibo Wu

Keyword(s):

Antioxidant Defense ◽

Defense Enzymes ◽

Rice Seedlings ◽

Dependent Effect ◽

Antioxidant Defense Enzymes ◽

Induced Changes

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Activities of antioxidant defense enzymes in the blood of individuals with Leu144Phe mutation

Jugoslovenska medicinska biohemija ◽

10.2298/jmh0502111n ◽

2005 ◽

Vol 24 (2) ◽

pp. 111-114 ◽

Cited By ~ 1

Author(s):

Aleksandra Nikolic ◽

Zorica Stevic ◽

Dusko Blagojevic ◽

Zorica Saicic ◽

Mihajlo Spasic

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Antioxidant Defense ◽

Familial Amyotrophic Lateral Sclerosis ◽

Defense Enzymes ◽

Glutathione S Transferase ◽

Zinc Superoxide Dismutase ◽

Antioxidant Defense Enzymes ◽

Discriminant Analyses ◽

Lateral Sclerosis

Activities of cooper zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of familial amyotrophic lateral sclerosis patients with Leu144Phe mutation (FALS), asimptomatic carriers with Leu144Phe mutation and controls were studied. Activity of Cu,Zn SOD was significantly lower in the FALS patients and asimptomatic carriers than in controls (p<0.001). In the FALS patients GSH-Px activity was lower (p<0.01) and activity of GR was higher (p<0.001) in comparison with controls. Canonical discriminant analyses provide statistical evidence that examined groups are different in the composition of antioxidant enzymes in blood and revealed that each component confers to observed difference. Our results suggests that oxidative stress is involved in pathogenesis of FALS and the activities of antioxidant enzymes are exposed to different kind of oxidative pressure in FALS patients, asymptomatic carriers and controls.

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Effects of age and strain on small intestinal and hepatic antioxidant defense enzymes in Wistar and Fisher 344 rats

Mechanisms of Ageing and Development ◽

10.1016/s0047-6374(01)00236-6 ◽

2001 ◽

Vol 122 (6) ◽

pp. 561-570 ◽

Cited By ~ 23

Author(s):

InSurk Jang ◽

KabRyong Chae ◽

JungSik Cho

Keyword(s):

Antioxidant Defense ◽

Defense Enzymes ◽

Antioxidant Defense Enzymes ◽

Small Intestinal

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Prospects for the use of highly effective and safe stimulants of embryogenesis in poultry farming

Veterinariya, Zootekhniya i Biotekhnologiya ◽

10.36871/vet.zoo.bio.202106007 ◽

2021 ◽

Vol 1 (6) ◽

pp. 42-50

Author(s):

I. I. Kochish ◽

◽

T. A. Sadovskaya ◽

T. O. Azarnova ◽

◽

...

Keyword(s):

Antioxidant Defense ◽

Morphological Changes ◽

Biologically Active ◽

Defense Enzymes ◽

Chick Embryos ◽

Negative Effects ◽

Resource Saving ◽

Antioxidant Defense Enzymes ◽

Active Substances ◽

Poultry Farming

To increase the embryonic and postembryonic viability of poultry, along with selection work, optimization of the microclimate parameters of poultry houses and rations, it is also advisable to develop resource-saving and environmentally friendly methods of using biologically active substances. It was shown that the treatment of eggs with a composition of solutions of natural metabolites (colamine, succinic acid and Ribav) in optimal concentrations stopped and prevented the negative effects of stress. Positive biochemical and morphological changes were observed in the development of chick embryos. An increase in chick withdrawal, a decrease in all incubation waste, an increase in the activity of antioxidant defense enzymes, and a decrease in the content of malondialdehyde in the blood serum of chickens were found.

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Molecular Role of Nitric Oxide in Secondary Products Production in Ginkgo biloba Cell Suspension Culture

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4319660 ◽

2015 ◽

Vol 43 (1) ◽

pp. 12-18 ◽

Cited By ~ 5

Author(s):

Hossam Saad EL-BELTAGI ◽

Osama K. AHMED ◽

Adel E. HEGAZY

Keyword(s):

Nitric Oxide ◽

Ginkgo Biloba ◽

Antioxidant Defense ◽

Nitric Oxide Donor ◽

Peat Moss ◽

Defense Enzymes ◽

Secondary Products ◽

Equal Concentration ◽

Shoot Height ◽

Antioxidant Defense Enzymes

Effects of sodium nitroprusside (SNP; nitric oxide donor) treatment on the enhancement of secondary metabolites production, oxidative stress mediators (O2-.) accumulation and antioxidant defense enzymes of Ginkgo biloba callus culture was investigated. On one hand, the obtained data showed a highly metabolic modification of chemical constituents, PAL activity and various antioxidant defense enzymes (APX, SOD), which gradually increased in response to SNP treatments. On the other hands the high NO levels significantly increased the accumulation of various oxidative burst of O2-.. MS basal medium supplemented with casein hydrolase (500 mg/L), NAA and BA at equal concentration (0.5 mg/L) recorded the highest number of regenerated shoots (4.81 cm) and shoot height (4.96 cm) as well as root number (2.25 cm) and root length (4.5 cm). The highest survival (40 %) was shown in acclimatization on the mixture containing sand, peat moss and vermiculite (1: 1: 1, v/v/v), which significantly confirmed and reflected the variation in survival percentage. Meanwhile, higher treatment (500 μM) of NO positively enhanced secondary products accumulation of total tannins, saponins, phenols and total flavonoids in G. biloba callus culture.

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Biosynthetic insulin effect on red cell membrane lipid peroxidation in patients with insulin-dependent diabetes

Problems of Endocrinology ◽

10.14341/probl12003 ◽

1994 ◽

Vol 40 (3) ◽

pp. 12-15 ◽

Cited By ~ 2

Author(s):

T. S. Balashova ◽

Ye. N. Golega ◽

I. A. Rudko ◽

M. I. Balabolkin ◽

A. A. Kubatiev

Keyword(s):

Cell Membrane ◽

Human Insulin ◽

Antioxidant Defense ◽

Vascular Complications ◽

Membrane Lipid ◽

Defense Enzymes ◽

Red Cell ◽

Membrane Lipid Peroxidation ◽

Red Cell Membrane ◽

Antioxidant Defense Enzymes

The aim of our research was elucidation of a relationship between red cell membrane lipid peroxidation (LPO) and antioxidant' defense enzymes, on the one hand and the age disease duration and presence of vascular complications in patients with type I diabetes mellitus on the other. The possibility of correcting red cell peroxide status with human insulin preparations was investigated. Red cell membrane LPO was found increased more than twofold and antioxidant defense enzymes activities virtually unchanged vs. controls in 16 patients with diabetes aged 20 to 43. These characteristics of red cell peroxidation status do not depend on patients age disease standing or presence of vascular complications. A twelve-week therapy with biosynthetic insulin resulted in complete normalization of LPO processes in patients with angiopathies aged under 35 and with disease standing of less than 10 years. In diabetics with angiopathies aged over 35 and disease standing of more than 10 years red cell MDA level reduced under the effect of therapy with human insulin preparations but was still increased vs. that in healthy donors by 1.5 times. Red cell GP and SOD activities reduced in the course of insulin therapy in all the examined groups of diabetics. Catalase activity increased by approximately 50% in patients with angiopathies those aged over 35 and a disease standing of more than 10 years under the effect of insulin. In the rest groups of patients catalase activity did not differ from its initial level. Our results permit us recommending besides human insulin preparations antioxidant therapy for patients with vascular complications those aged over 35 and a disease standing of more than 10 years.

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