scholarly journals Activities of antioxidant defense enzymes in the blood of individuals with Leu144Phe mutation

2005 ◽  
Vol 24 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Aleksandra Nikolic ◽  
Zorica Stevic ◽  
Dusko Blagojevic ◽  
Zorica Saicic ◽  
Mihajlo Spasic
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Antioxidant Defense ◽  
Familial Amyotrophic Lateral Sclerosis ◽  
Defense Enzymes ◽  
Glutathione S Transferase ◽  
Zinc Superoxide Dismutase ◽  
Antioxidant Defense Enzymes ◽  
Discriminant Analyses ◽  
Lateral Sclerosis

Activities of cooper zinc superoxide dismutase (Cu,Zn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of familial amyotrophic lateral sclerosis patients with Leu144Phe mutation (FALS), asimptomatic carriers with Leu144Phe mutation and controls were studied. Activity of Cu,Zn SOD was significantly lower in the FALS patients and asimptomatic carriers than in controls (p<0.001). In the FALS patients GSH-Px activity was lower (p<0.01) and activity of GR was higher (p<0.001) in comparison with controls. Canonical discriminant analyses provide statistical evidence that examined groups are different in the composition of antioxidant enzymes in blood and revealed that each component confers to observed difference. Our results suggests that oxidative stress is involved in pathogenesis of FALS and the activities of antioxidant enzymes are exposed to different kind of oxidative pressure in FALS patients, asymptomatic carriers and controls.

scholarly journals Oxidative stress and genetic markers of suboptimal antioxidant defense in the aging brain: a theoretical review

2014 ◽  
Vol 25 (6) ◽  
Author(s):  
Lauren E. Salminen ◽  
Robert H. Paul
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Future Research ◽  
Aging Brain ◽  
Older Individuals ◽  
Glutathione S Transferase ◽  
Antioxidant Defense Enzymes ◽  
Repair Mechanisms ◽  
The Impact ◽  
The Brain

AbstractNormal aging involves a gradual breakdown of physiological processes that leads to a decline in cognitive functions and brain integrity, yet the onset and progression of decline are variable among older individuals. While many biological changes may contribute to this degree of variability, oxidative stress is a key mechanism of the aging process that can cause direct damage to cellular architecture within the brain. Oligodendrocytes are at a high risk for oxidative damage due to their role in myelin maintenance and production and limited repair mechanisms, suggesting that white matter may be particularly vulnerable to oxidative activity. Antioxidant defense enzymes within the brain, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST), are crucial for breaking down the harmful end products of oxidative phosphorylation. Previous studies have revealed that allele variations of polymorphisms that encode these antioxidants are associated with abnormalities in SOD, CAT, GPx, and GST activity in the central nervous system. This review will focus on the role of oxidative stress in the aging brain and the impact of decreased antioxidant defense on brain integrity and cognitive function. Directions for future research investigations of antioxidant defense genes will also be discussed.

scholarly journals Effect of melatonin on antioxidant enzyme activities in blood erythrocytes of rats during acute emotional stress

2015 ◽  
Vol 61 (3) ◽  
pp. 394-399 ◽  
Author(s):  
S.S. Pertsov ◽  
L.S. Kalinichenko ◽  
E.V. Koplik ◽  
L.G. Nagler ◽  
E.S. Alinkina ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Antioxidant Enzyme ◽  
Emotional Stress ◽  
Peripheral Blood ◽  
Functional Activity ◽  
Antioxidant Defense ◽  
Defense Enzymes ◽  
Experimental Stress ◽  
Antioxidant Defense Enzymes ◽  
Induced Changes

The effect of the epiphyseal hormone melatonin on the activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), and Cu/Zn-superoxide dismutase (Cu/Zn-SOD) was studied in peripheral blood erythrocytes of behaviorally passive and active Wistar rats. Acute emotional stress was modeled by immobilization of animals for1 h with simultaneous electrocutaneous stimulation. Basal activity of antioxidant glutathione enzymes in erythrocytes of behaviorally passive rats was higher than that in active animals. Administration of melatonin (2 mg/kg, intraperitoneally) was accompanied by a decrease in the activity of GPx and GR in erythrocytes from non-stressed passive animals. After experimental stress, passive rats demonstrated a significant increase in the activity of Cu/Zn-SOD and GPx in peripheral blood erythrocytes. The absence of stress-induced changes in functional activity of antioxidant defense enzymes in the blood of behaviorally active animals suggests a relatively constant oxidative status of tissues in these animals under stress conditions. Melatonin administration had little effect on stress-induced changes in functional activity of the erythrocyte antioxidant system in passive rats. Active specimens pretreated with melatonin before stress exposure were characterized by activation of study antioxidant enzymes. Quantitative parameters of the erythrocyte antioxidant defense enzymes did not differ in behaviorally active and passive rats subjected to experimental stress after melatonin injection. Thus, exogenous melatonin abolishes differences in the activity of study antioxidant enzymes in erythrocytes of animals with different behavioral parameters under basal conditions and after experimental stress. In passive rats melatonin mainly reduced the initial tension of oxidative processes. By contrast, administration of this hormone to active specimens is followed by an increase in functional activity of the antioxidant enzyme system under conditions of acute stress.

scholarly journals Experimental Protoporphyria: Effect of Bile Acids on Liver Damage Induced by Griseofulvin

2015 ◽  
Vol 2015 ◽  
pp. 1-10
Author(s):  
María del Carmen Martinez ◽  
Silvina Fernanda Ruspini ◽  
Susana Graciela Afonso ◽  
Roberto Meiss ◽  
Ana Maria Buzaleh ◽  
...  
Keyword(s):  
Bile Acids ◽  
Liver Damage ◽  
Antioxidant Defense ◽  
Defense Enzymes ◽  
Mice Model ◽  
Glutathione S Transferase ◽  
Gamma Glutamyl Transpeptidase ◽  
Antioxidant Defense Enzymes ◽  
Glutamyl Transpeptidase ◽  
And Oxidative Stress

The effect of bile acids administration to an experimental mice model of Protoporphyria produced by griseofulvin (Gris) was investigated. The aim was to assess whether porphyrin excretion could be accelerated by bile acids treatment in an attempt to diminish liver damage induced by Gris. Liver damage markers, heme metabolism, and oxidative stress parameters were analyzed in mice treated with Gris and deoxycholic (DXA), dehydrocholic (DHA), chenodeoxycholic, or ursodeoxycholic (URSO). The administration of Gris alone increased the activities of glutathione reductase (GRed), superoxide dismutase (SOD), alkaline phosphatase (AP), gamma glutamyl transpeptidase (GGT), and glutathione-S-transferase (GST), as well as total porphyrins, glutathione (GSH), and cytochrome P450 (CYP) levels in liver. Among the bile acids studied, DXA and DHA increased PROTO IX excretion, DXA also abolished the action of Gris, reducing lipid peroxidation and hepatic GSH and CYP levels, and the activities of GGT, AP, SOD, and GST returned to control values. However, porphyrin accumulation was not prevented by URSO; instead this bile acid reduced ALA-S and the antioxidant defense enzymes system activities. In conclusion, we postulate that DXA acid would be more effective to prevent liver damage induced by Gris.

scholarly journals Role of Oxidative Stress in the Pathogenesis of Amyotrophic Lateral Sclerosis: Antioxidant Metalloenzymes and Therapeutic Strategies

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 437
Author(s):  
Pavlína Hemerková ◽  
Martin Vališ
Keyword(s):  
Oxidative Stress ◽  
Amyotrophic Lateral Sclerosis ◽  
Antioxidant Enzymes ◽  
Oxygen Radicals ◽  
Clinical Symptoms ◽  
Free Oxygen Radicals ◽  
Free Oxygen ◽  
Sporadic Als ◽  
Familial Form ◽  
Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) affects motor neurons in the cerebral cortex, brainstem and spinal cord and leads to death due to respiratory failure within three to five years. Although the clinical symptoms of this disease were first described in 1869 and it is the most common motor neuron disease and the most common neurodegenerative disease in middle-aged individuals, the exact etiopathogenesis of ALS remains unclear and it remains incurable. However, free oxygen radicals (i.e., molecules containing one or more free electrons) are known to contribute to the pathogenesis of this disease as they very readily bind intracellular structures, leading to functional impairment. Antioxidant enzymes, which are often metalloenzymes, inactivate free oxygen radicals by converting them into a less harmful substance. One of the most important antioxidant enzymes is Cu2+Zn2+ superoxide dismutase (SOD1), which is mutated in 20% of cases of the familial form of ALS (fALS) and up to 7% of sporadic ALS (sALS) cases. In addition, the proper functioning of catalase and glutathione peroxidase (GPx) is essential for antioxidant protection. In this review article, we focus on the mechanisms through which these enzymes are involved in the antioxidant response to oxidative stress and thus the pathogenesis of ALS and their potential as therapeutic targets.

Prospects for the use of highly effective and safe stimulants of embryogenesis in poultry farming

2021 ◽  
Vol 1 (6) ◽  
pp. 42-50
Author(s):  
I. I. Kochish ◽  
◽  
T. A. Sadovskaya ◽  
T. O. Azarnova ◽  
◽  
...  
Keyword(s):  
Antioxidant Defense ◽  
Morphological Changes ◽  
Biologically Active ◽  
Defense Enzymes ◽  
Chick Embryos ◽  
Negative Effects ◽  
Resource Saving ◽  
Antioxidant Defense Enzymes ◽  
Active Substances ◽  
Poultry Farming

To increase the embryonic and postembryonic viability of poultry, along with selection work, optimization of the microclimate parameters of poultry houses and rations, it is also advisable to develop resource-saving and environmentally friendly methods of using biologically active substances. It was shown that the treatment of eggs with a composition of solutions of natural metabolites (colamine, succinic acid and Ribav) in optimal concentrations stopped and prevented the negative effects of stress. Positive biochemical and morphological changes were observed in the development of chick embryos. An increase in chick withdrawal, a decrease in all incubation waste, an increase in the activity of antioxidant defense enzymes, and a decrease in the content of malondialdehyde in the blood serum of chickens were found.

scholarly journals Molecular Role of Nitric Oxide in Secondary Products Production in Ginkgo biloba Cell Suspension Culture

2015 ◽  
Vol 43 (1) ◽  
pp. 12-18 ◽  
Author(s):  
Hossam Saad EL-BELTAGI ◽  
Osama K. AHMED ◽  
Adel E. HEGAZY
Keyword(s):  
Nitric Oxide ◽  
Ginkgo Biloba ◽  
Antioxidant Defense ◽  
Nitric Oxide Donor ◽  
Peat Moss ◽  
Defense Enzymes ◽  
Secondary Products ◽  
Equal Concentration ◽  
Shoot Height ◽  
Antioxidant Defense Enzymes

Effects of sodium nitroprusside (SNP; nitric oxide donor) treatment on the enhancement of secondary metabolites production, oxidative stress mediators (O2-.) accumulation and antioxidant defense enzymes of Ginkgo biloba callus culture was investigated. On one hand, the obtained data showed a highly metabolic modification of chemical constituents, PAL activity and various antioxidant defense enzymes (APX, SOD), which gradually increased in response to SNP treatments. On the other hands the high NO levels significantly increased the accumulation of various oxidative burst of O2-.. MS basal medium supplemented with casein hydrolase (500 mg/L), NAA and BA at equal concentration (0.5 mg/L) recorded the highest number of regenerated shoots (4.81 cm) and shoot height (4.96 cm) as well as root number (2.25 cm) and root length (4.5 cm). The highest survival (40 %) was shown in acclimatization on the mixture containing sand, peat moss and vermiculite (1: 1: 1, v/v/v), which significantly confirmed and reflected the variation in survival percentage. Meanwhile, higher treatment (500 μM) of NO positively enhanced secondary products accumulation of total tannins, saponins, phenols and total flavonoids in G. biloba callus culture.

scholarly journals Biosynthetic insulin effect on red cell membrane lipid peroxidation in patients with insulin-dependent diabetes

1994 ◽  
Vol 40 (3) ◽  
pp. 12-15 ◽  
Author(s):  
T. S. Balashova ◽  
Ye. N. Golega ◽  
I. A. Rudko ◽  
M. I. Balabolkin ◽  
A. A. Kubatiev
Keyword(s):  
Cell Membrane ◽  
Human Insulin ◽  
Antioxidant Defense ◽  
Vascular Complications ◽  
Membrane Lipid ◽  
Defense Enzymes ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Red Cell Membrane ◽  
Antioxidant Defense Enzymes

The aim of our research was elucidation of a relationship between red cell membrane lipid peroxidation (LPO) and antioxidant' defense enzymes, on the one hand and the age disease duration and presence of vascular complications in patients with type I diabetes mellitus on the other. The possibility of correcting red cell peroxide status with human insulin preparations was investigated. Red cell membrane LPO was found increased more than twofold and antioxidant defense enzymes activities virtually unchanged vs. controls in 16 patients with diabetes aged 20 to 43. These characteristics of red cell peroxidation status do not depend on patients age disease standing or presence of vascular complications. A twelve-week therapy with biosynthetic insulin resulted in complete normalization of LPO processes in patients with angiopathies aged under 35 and with disease standing of less than 10 years. In diabetics with angiopathies aged over 35 and disease standing of more than 10 years red cell MDA level reduced under the effect of therapy with human insulin preparations but was still increased vs. that in healthy donors by 1.5 times. Red cell GP and SOD activities reduced in the course of insulin therapy in all the examined groups of diabetics. Catalase activity increased by approximately 50% in patients with angiopathies those aged over 35 and a disease standing of more than 10 years under the effect of insulin. In the rest groups of patients catalase activity did not differ from its initial level. Our results permit us recommending besides human insulin preparations antioxidant therapy for patients with vascular complications those aged over 35 and a disease standing of more than 10 years.

Antioxidant defenses and metabolic depression in a pulmonate land snail

1995 ◽  
Vol 268 (6) ◽  
pp. R1386-R1393 ◽  
Author(s):  
M. Hermes-Lima ◽  
K. B. Storey
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Thiobarbituric Acid ◽  
Land Snail ◽  
Land Snails ◽  
Antioxidant Defenses ◽  
Glutathione S Transferase ◽  
Hypometabolic State

During arousal from estivation oxygen consumption by land snails (Otala lactea) increases severalfold. To determine whether snails prepared for an accompanying rise in the rates of oxyradical generation by altering their antioxidant defense mechanisms, changes in the activities of antioxidant enzymes and lipid peroxidation products were quantified in foot and hepatopancreas of control, 30-day estivating, and aroused snails. Compared with controls, estivating O. lactea showed significant increases in the activities of foot muscle superoxide dismutase (SOD) (increasing by 56-67%), catalase (51-72%), and glutathione S-transferase (79-108%), whereas, in hepatopancreas, SOD (57-78%) and glutathione peroxidase (93-144%) increased. Within 40 min after arousal began, hepatopancreas glutathione peroxidase activity had returned to control values, but SOD showed a further 70% increase in activity but then returned to control levels by 80 min. Estivation had no effect on total glutathione (GSH + 2 GSSG) concentrations in tissues, but GSSG content had increased about twofold in both organs of 30-day dormant snails. Lipid peoxidation (quantified as thiobarbituric acid reactive substances) was significantly enhanced at the onset of arousal from dormancy, indicating that oxidative stress and tissue damage occurred at this time. The data suggest that antioxidant defenses in snail organs are increased while snails are in the hypometabolic state as a preparation for oxidative stress during arousal.

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