scholarly journals Mammaglobin in peripheral blood and tumor in breast cancer patients

2016 ◽  
Vol 62 (4) ◽  
pp. 453-457
Author(s):  
V.K. Bozhenko ◽  
N.V. Kharchenko ◽  
E.F. Vaskevich ◽  
E.A. Kudinova ◽  
A.V. Oorzhak ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Breast Cancer ◽  
Sensitivity And Specificity ◽  
Peripheral Blood ◽  
Expression Level ◽  
Breast Cancer Patients ◽  
Tissue Samples ◽  
Specificity And Sensitivity ◽  
Healthy Donors ◽  
Maximal Expression

Currently, no molecular biological markers do exist for early diagnosis of breast cancer. One of the possible candidates for the marker of early breast cancer is mammaglobin (MGB1) or SCGB2A2 (secretoglobin, family 2A, member 2), characterized by the maximal expression level in early breast cancer. Using the RT-PCR method MGB1 mRNA expression was examined in 57 tumor tissue samples and 57 samples of morphologically non-malignant tissue (MNT) of breast cancer (BC) patients. Specificity and sensitivity of the MGB1 mRNA assay in peripheral blood of BC patients was evaluated by nested PCR. 169 blood samples (from 95 BC patients, 22 from patients with benign breast tumors, 28 from patients with tumors of other localizations, and 24 samples from healthy donors) have been analyzed. MGB1 expression was significantly higher in BC tissue samples compared to MNT (p=0.0019). The maximal expression level was in the samples T1 (p=0.013), stage I BC (p=0.037), GI (p=0.0019). The MGB1 expression positively correlated with expression of estrogen (p = 0,034) and progesterone (p=0.0004) receptors. Sensitivity and specificity of the MGB1 mRNA assay in peripheral blood were 60.6% and 92.3%, respectively. Expression of MGB1 was higher in BC than MNT and it decreased during BC progression. The sensitivity and specificity of the MGB1 mRNA assay may be used as an additional diagnostic method.

scholarly journals RT-PCR determination of maspin and mammaglobin B in peripheral blood of healthy donors and breast cancer patients

2006 ◽  
Vol 17 (3) ◽  
pp. 424-428 ◽  
Author(s):  
L. Mercatali ◽  
V. Valenti ◽  
D. Calistri ◽  
S. Calpona ◽  
G. Rosti ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Breast Cancer Patients ◽  
Rt Pcr ◽  
Healthy Donors

scholarly journals Does HOTAIR expression level in the peripheral blood have veritably predictive/prognostic impact on breast cancer patients?

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Vahid Ezzatizadeh ◽  
Hossein Mozdarani
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Expression Level ◽  
Prognostic Impact ◽  
Breast Cancer Patients ◽  
Blood Biomarker ◽  
Non Coding Rna ◽  
Before And After ◽  
Significant Expression

AbstractAs a peripheral blood biomarker, the crucial role of long non-coding RNA (lncRNA) HOTAIR has recently been suggested in many types of disorder. Among these reports, few investigations have indicated overexpression of HOTAIR transcript in the breast cancer patients’ peripheral blood. In this regard, we studied the potential impact of radiotherapy on the peripheral blood HOTAIR expression of different breast cancer patients. Curiously, no significant expression level of HOTAIR was determined in the breast cancer patients’ peripheral blood, before and after radiotherapy (10 Gy exposure). Deliberating these investigations raised some debates on the specificity of the utilized methods, the corresponding obtained findings and impact of HOTAIR expression on breast cancer predication, as a potential peripheral blood biomarker, which is discussed in this article.

scholarly journals Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769504 ◽  
Author(s):  
Golnaz Khakpour ◽  
Mehrdad Noruzinia ◽  
Pantea Izadi ◽  
Fatemeh Karami ◽  
Mohammad Ahmadvand ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Young Patients ◽  
Breast Cancer Patients ◽  
Tissue Samples ◽  
Non Invasive ◽  
Cancer Pathogenesis

Critical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was performed on DNA samples isolated from white blood cells of 30 breast cancer patients compared to 30 healthy controls. A total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction. Hypermethylation of APC, HDAC1, and GSK1 genes has been previously reported in more than one study on tissue samples of breast cancer. Methylation of those aforementioned genes in white blood cells of our young patients not only relies on their importance in breast cancer pathogenesis but also may highlight their potential as early epimarkers that makes further assessments necessary in large cohort studies.

scholarly journals Interplay between copy number alterations and immune profiles in the early breast cancer Scandinavian Breast Group 2004-1 randomized phase II trial: results from a feasibility study

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ioannis Zerdes ◽  
Michele Simonetti ◽  
Alexios Matikas ◽  
Luuk Harbers ◽  
Balazs Acs ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Breast Cancer ◽  
Phase Ii ◽  
Copy Number ◽  
Immune Cell ◽  
Phase Ii Trial ◽  
Lymphocytic Infiltration ◽  
Ffpe Tissue ◽  
Breast Cancer Patients ◽  
Tissue Samples

AbstractEmerging data indicate that genomic alterations can shape immune cell composition in early breast cancer. However, there is a need for complementary imaging and sequencing methods for the quantitative assessment of combined somatic copy number alteration (SCNA) and immune profiling in pathological samples. Here, we tested the feasibility of three approaches—CUTseq, for high-throughput low-input SCNA profiling, multiplexed fluorescent immunohistochemistry (mfIHC) and digital-image analysis (DIA) for quantitative immuno-profiling- in archival formalin-fixed paraffin-embedded (FFPE) tissue samples from patients enrolled in the randomized SBG-2004-1 phase II trial. CUTseq was able to reproducibly identify amplification and deletion events with a resolution of 100 kb using only 6 ng of DNA extracted from FFPE tissue and pooling together 77 samples into the same sequencing library. In the same samples, mfIHC revealed that CD4 + T-cells and CD68 + macrophages were the most abundant immune cells and they mostly expressed PD-L1 and PD-1. Combined analysis showed that the SCNA burden was inversely associated with lymphocytic infiltration. Our results set the basis for further applications of CUTseq, mfIHC and DIA to larger cohorts of early breast cancer patients.

Peripheral blood NK cells expressing HLA-G, IL-10 and TGF-β in healthy donors and breast cancer patients

2015 ◽  
Vol 298 (1-2) ◽  
pp. 37-46 ◽  
Author(s):  
Yekaterina O. Ostapchuk ◽  
Esin Aktas Cetin ◽  
Yuliya V. Perfilyeva ◽  
Abdullah Yilmaz ◽  
Yuriy A. Skiba ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nk Cells ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Breast Cancer Patients ◽  
Healthy Donors

scholarly journals Polyfunctional KLRG-1+CD57+ Senescent CD4+ T Cells Infiltrate Tumors and Are Expanded in Peripheral Blood From Breast Cancer Patients

2021 ◽  
Vol 12 ◽  
Author(s):  
Maria C. Ramello ◽  
Nicolás G. Núñez ◽  
Jimena Tosello Boari ◽  
Sabrina N. Bossio ◽  
Fernando P. Canale ◽  
...  
Keyword(s):  
Breast Cancer ◽  
T Cells ◽  
T Cell ◽  
Peripheral Blood ◽  
Transcriptional Profiling ◽  
Receptor Expression ◽  
Breast Cancer Patients ◽  
Chronic Infections ◽  
Healthy Donors ◽  
Effector Cytokines

Senescent T cells have been described during aging, chronic infections, and cancer; however, a comprehensive study of the phenotype, function, and transcriptional program of this T cell population in breast cancer (BC) patients is missing. Compared to healthy donors (HDs), BC patients exhibit an accumulation of KLRG-1+CD57+ CD4+ and CD8+ T cells in peripheral blood. These T cells infiltrate tumors and tumor-draining lymph nodes. KLRG-1+CD57+ CD4+ and CD8+ T cells from BC patients and HDs exhibit features of senescence, and despite their inhibitory receptor expression, they produce more effector cytokines and exhibit higher expression of Perforin, Granzyme B, and CD107a than non-senescent subsets. When compared to blood counterparts, tumor-infiltrating senescent CD4+ T cells show similar surface phenotype but reduced cytokine production. Transcriptional profiling of senescent CD4+ T cells from the peripheral blood of BC patients reveals enrichment in genes associated with NK or CD8+-mediated cytotoxicity, TCR-mediated stimulation, and cell exhaustion compared to non-senescent T cells. Comparison of the transcriptional profile of senescent CD4+ T cells from peripheral blood of BC patients with those of HDs highlighted marked similarities but also relevant differences. Senescent CD4+ T cells from BC patients show enrichment in T-cell signaling, processes involved in DNA replication, p53 pathways, oncogene-induced senescence, among others compared to their counterparts in HDs. High gene expression of CD4, KLRG-1, and B3GAT1 (CD57), which correlates with increased overall survival for BC patients, underscores the usefulness of the evaluation of the frequency of senescent CD4+ T cells as a biomarker in the follow-up of patients.

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