scholarly journals The influence of slowly and rapidly degradable concentrate protein on a number of rumen parameters in dairy cattle.

1986 ◽  
Vol 34 (2) ◽  
pp. 199-216
Author(s):  
W.A.G. Veen

In 5 trials with Dutch Black Pied or Dutch Black Pied X Holstein-Friesian cows the effects of feeding concentrates with rapidly and slowly degradable protein on rumen values were investigated. Samples of rumen fluid were taken before and 45, 90, 135 and 180 min after the morning concentrate feed. Rumen pH was not affected by protein degradability. Rumen ammonia content was higher when easily degradable protein was given than when slowly degradable protein was given; peak concentration varied from 10 to 12.5 and from 4 to 8 mmol/litre, respectively. Maximum concentration of lactate was not influenced by protein degradability. Rumen content of volatile fatty acids increased more rapidly when the slowly degradable protein rather than when the rapidly degradable protein was given. At all sampling times the molar acetate:propionate ratio was higher after feeding on slowly degradable protein than after rapidly degradable protein. (Abstract retrieved from CAB Abstracts by CABI’s permission)

1982 ◽  
Vol 54 (2) ◽  
pp. 127-135
Author(s):  
Liisa Syrjälä-Qvist

The criteria used in comparing the utilization of grass silage by reindeer and sheep were rumen pH, ammonia, volatile fatty acids (VFA) and microbes. Rumen samples were taken before feeding, and 2 ½ and 5 ½ hours after the beginning of feeding. Rumen fermentation was lower in the reindeer than in the sheep and differed less between the three sampling times. In the reindeer/the pH of the rumen fluid averaged 6.94 and in the sheep 6.61. The average amounts of NH3—N were 17.0 and 24.2 mg/100 ml rumen fluid and those of total VFA 8.46 and 10.90 mmoles/100ml rumen fluid, respectively. The proportion of acetic acid in the VFA in the reindeer was 75.3 molar % and in the sheep 66.0 molar %, the corresponding values for propionic acid being 18.5 and 22.0 molar % and for butytic acid 4.2 and 8.8 molar %. The number of rumen ciliates in the reindeer averaged 87/mm3 rumen contents and in the sheep 314/ mm3. The numbers of bacteria were 16.0 X 106/mm3, respectively. The proportion of the total microbe mass in the reindeer rumen contents was 1.8 % and in the sheep 2.4 %. The proportions of bacteria in this mass were 87 % and 70 %, respectively. The differences between the reindeer and sheep in the rumen fermentation results and in the numbers of rumen microbiota were nearly all statistically significant (P


1988 ◽  
Vol 36 (2) ◽  
pp. 127-143 ◽  
Author(s):  
W.A.G. Veen ◽  
J. Veling ◽  
Y.T. Bakker

In a crossover trial, 4 cows were given concentrates containing rapidly and slowly degradable protein in combination with prewilted grass silage. Diets were given in 2 equal daily portions according to DCP and net energy requirements. The trial consisted of 3 main periods of 3 weeks each, with faeces, urine and milk collected and measured during the final week. On 1 day during this week samples of rumen fluid were taken and on 2 days blood samples were taken, directly before the morning feed and 1, 2, 3, 4 and 8 h later. N digestibity and N retention were the same on both rations. A concentrate with slowly-degradable protein resulted in a significantly higher rumen pH and acetate:propionate ratio. Ammonia concn. tended to be lower. Slowly-degradable concentrate protein led to a significantly higher concn. of urea and a lower concn. of insulin in blood. The concn. of several of the essential amino acids, and of the glucogenic amino acids and glycine was significantly lower. There were no significant effects of type of protein on milk yield or milk composition, but there was a tendency for these parameters to increase with ration containing slowly-degradable protein. Results suggest the ration containing slowly-degradable concentrate protein produced a more even rumen fermentation, which promoted a higher acetate:propionate ration in the rumen. On this ration there was a tendency for more gluconeogenesis from amino acids to occur. (Abstract retrieved from CAB Abstracts by CABI’s permission)


1972 ◽  
Vol 27 (2) ◽  
pp. 425-437 ◽  
Author(s):  
F. G. Whitelaw ◽  
J. Margaret Eadie ◽  
S. O. Mann ◽  
R. S. Reid

1. Two pairs of Friesian steers were changed fromad lib.to restricted intake of a pelleted barley diet and were maintained on this latter intake for periods of 18 or 25 weeks. The restricted level of intake was 70 g/kg0.73and was adjusted weekly according to individual live weights. The daily allowance was given in three equal feeds during day-time.2. After an initial period of 9 weeks on the restricted diet, during which all four animals were kept free of rumen ciliate protozoa, one member of each pair was given an inoculum of rumen ciliates. Eight weeks later, the ciliate-free member of the younger pair of steers was similarly inoculated. Observations were made on the rumen bacterial and protozoal populations and on changes in rumen pH and volatile fatty acids (VFA) throughout each treatment period. The concentrations of urea and haemoglobin in blood and of glucose and amino acids in plasma were examined on one occasion in each animal.3. In the absence of ciliates, restriction of intake resulted in rumen pH values and molar proportions of VFA similar to those normally encountered on anad lib.intake of a barley diet. A decrease in bacterial numbers and certain minor changes in bacterial types were observed on changing fromad lib.to restricted intake but the resultant population under ciliate-free conditions was basically the same as that found later in the faunated animals. In culture, organisms of the genusBacteroideswere predominant.4. Large populations of rumen ciliates were established in each animal inoculated. Relative to the ciliate-free periods, the presence of ciliates resulted in an increase in rumen pH, a reduction in total VFA concentration and a decrease in the ratio of propionic to butyric acid in rumen fluid. It is concluded that these changes are a direct effect of ciliate activity.5. Conditions within the rumen remained more stable from day to day when large ciliate populations were present than when ciliates were absent. In one animal, spontaneous fluctuations in ciliate number were accompanied by corresponding changes in rumen pH and VFA proportions.6. Significant differences were observed between faunated and ciliate-free animals in the concentration urea in blood and of glucose in plasma; only minor differences were noted in blood haemoglobin and plasma amino acid concentrations.


1970 ◽  
Vol 12 (3) ◽  
pp. 503-512 ◽  
Author(s):  
P. Jackson ◽  
J. A. F. Rook

SUMMARYThe effect of introducing a solution of fatty acids (consisting mainly of ammonium acetate) into the drinking water of Jersey and Friesian cows on the yield and composition of milk was investigated. In short-term experiments there were small increases in milk yield and in the yield of fat in Jersey cows receiving either a high-roughage or a high-concentrate diet and in Friesian cows receiving a high-concentrate diet. Friesian cows receiving a high-roughage diet gave no response. In a long-term experiment extending over 16 weeks of lactation with Jersey cows receiving a high-concentrate diet, there was no immediate effect on milk yield but a greater persistency and overall the yield of fat was increased by 5·9%.Inclusion of ammonia salts in the drinking water caused increases in the ammonia contents of rumen liquor and of blood but there was little effect on rumen pH or the volatile fatty acids of rumen liquor.


1970 ◽  
Vol 24 (3) ◽  
pp. 653-660 ◽  
Author(s):  
Bijan Emmanuel ◽  
M. J. Lawlor ◽  
D. McAleese

1. Two wethers fitted with permanent rumen cannulas were used to study the effect of two mineral supplements, Na2HPO4 and a 1:1 mixture of Na2CO3 and NaHCO3, on the buffering system in the rumen of sheep given a pelleted roughage-concentrate diet.2. Measurements were made of rumen pH, buffering capacity value (β), total volatile fatty acids (VFA), CO2 and inorganic phosphorus concentrations as well as rumen cellulase activity.3. The addition of either mineral supplement to the diet significantly (P < 0.001) increased the rumen pH values during the critical post-feeding period, there was a difference of only 0.5 pH unit between the minimum and maximum pH values.4. Both mineral supplements significantly increased the concentrations of CO2 in the rumen fluid and rumen cellulase activity. They resulted in a general reduction in total VFA concentrations. It is suggested that this may possibly arise from increased absorption of VFA associated with increased CO2 tension in the rumen fluid.5. The Na2HPO4 supplement significantly ( P < 0.001) increased the β values in the pH range 6–8, whereas the bicarbonate supplement contributed to raising the β values in the pH range 5–6.6. The bicarbonate supplements raised cellulose digestibility from 39.7% for the control diet to 45.6% and the addition of Na2HPO4 further raised the digestibility to 51.1%. It would appear that low rumen pH impairs rumen cellulase activity and this could be offset by the addition of mineral supplements.


2006 ◽  
Vol 74 (1) ◽  
pp. 34-39 ◽  
Author(s):  
John R Roche ◽  
Dawn E Dalley ◽  
Frank P O'Mara

Reducing the dietary cation-anion difference (DCAD) has been shown to be an effective means of preventing parturient paresis in confinement systems where cows are offered a total mixed ration containing DCAD-reducing mineral compounds (anionic salts). Such a supplementation strategy is not possible in cows being group fed forages precalving, and little is known about the effect of supplementing these cows with large amounts of anionic salts twice daily.Eight non-lactating, pregnant Holstein-Friesian cows were allocated to two levels of DCAD (−20 and +18 meq/100 g DM) for 24 d, with an intensive Ca balance undertaken in metabolism stalls following a 2-week acclimatization to diet. The basal diet was 3 kg DM of crushed barley and 7 kg DM of pasture-hay. Urine and faeces were collected separately, weighed daily for 5 d and analysed for Ca content. Urinary Ca, creatinine and hydroxyproline concentration and plasma Ca concentration were determined during the period of the balance study. The diurnal pattern in urine and rumen pH was determined over 2 d. Decreasing DCAD reduced (P<0·001) the pH of urine, and increased (P<0·05) Ca absorption. Plasma Ca concentration was not affected by DCAD, and DCAD did not affect the output of urinary hydroxyproline, a marker of bone resorption. Twice-daily supplementation of anionic salts was sufficient to reduce the pH of blood and increase gastrointestinal Ca absorption. There was no diurnal variation in the pH of urine, suggesting that time of sampling to determine efficacy of DCAD in reducing systemic pH was not important.


2020 ◽  
Author(s):  
Jianxin Xiao ◽  
Muhammad Zahoor Khan ◽  
Gibson Maswayi Alugongo ◽  
Wei Wang ◽  
Yajing Wang ◽  
...  

Abstract This study aimed to investigate the short and long-term effect of early in life exposure to different feed sources on feed sorting and rumen fermentation of calves. Forty newborn female Holstein calves were randomly divided based on supplementation of concentrate (CON) or hay (HAY) only during the milk-feeding stage (d 1 to 56). After that, all calves were offered a total mixed ration (TMR) containing 43% of forage and 57% of concentrate until the end of the experiment (d 57 to 196). Calves were weighed immediately after birth and raised in individual hutches until d 70. The calves were then transferred to a heifer barn, housed within treatment in pairs (2 calves/pen). Rectal temperature, feed intake, body weight, body structural measurement, fecal and cough score were recorded ranges from day 1 to 196. Fresh and orts feed were sampled daily at the first two weeks (d 57-70) once TMR was offered and at the last week of the experiment (d 190 to 196) for analysis of feed sorting. Rumen fluid samples were collected at ranges from d 14-190 for detecting rumen pH and volatile fatty acids (VFA) concentrations. Our findings showed that early feed exposure did not influence calf health and growth performance but affected feed sorting in a short period. Upon transition to the same mixed diet, differences were found between treatments in the sorting of the different particle fractions. At week 9 and 10, calves early exposed to concentrates began to sort for fine particle fraction (primarily concentrate in this fraction), and against the long particle fraction (solely forage in this fraction), whereas calves early fed hay sorted for the long particle fraction, and against the fine particle fraction. However, no carryover effect was found, the differences between treatments fade away when detecting feed sorting at the last week, all calves sorted for short and fine particle fractions, and sort against long and medium particle fractions. Although the pattern of feed sorting initially differed between treatments, the rumen pH and VFA concentration were not altered once all calves transitioned to a TMR. These results suggested that the feed familiarity established early in life would not affect diet selection and rumen fermentation in heifer later in life, the dietary experience after transition to same TMR would eventually override the effect of early feed experience.


2020 ◽  
Author(s):  
Jianxin Xiao ◽  
Muhammad Zahoor Khan ◽  
Gibson Maswayi Alugongo ◽  
Wei Wang ◽  
Yajing Wang ◽  
...  

Abstract This study aimed to investigate the short and long-term effect of early in life exposure to different feed sources on feed sorting and rumen fermentation of calves. Forty newborn female Holstein calves were randomly divided based on supplementation of concentrate (CON) or hay (HAY) only during the milk-feeding stage (d 1 to 56). After that, all calves were offered a total mixed ration (TMR) containing 43 % of forage and 57 % of concentrate until the end of the experiment (d 57 to 196). Rectal temperature, feed intake, body weight, body structural measurement, fecal and cough score were recorded ranges from day 1 to 196. Fresh and orts feed were sampled daily at the first two weeks (d 57-70) once TMR was offered and at the last week of the experiment (d 190 to 196) for analysis of feed sorting. Rumen fluid samples were collected at ranges from d 14-190 for detecting rumen pH and volatile fatty acids (VFA) concentrations. Our findings showed that early feed exposure did not influence calf health and growth performance but affected feed sorting in a short period. Upon transition to the same mixed diet, differences were found between treatments in the sorting of the different particle fractions. At week 9 and 10, calves early exposed to concentrates began to sort for fine particle fraction, and against the long particle fraction, whereas calves early fed hay sorted for the long particle fraction, and against the fine particle fraction. However, no carryover effect was found, the differences between treatments fade away when detecting feed sorting at the last week, all calves sorted for short and fine particle fractions, and sort against long and medium particle fractions. Although the pattern of feed sorting initially differed between treatments, the rumen pH and VFA concentration were not altered once all calves transitioned to a TMR. These results suggested that the feed familiarity established early in life would not affect diet selection and rumen fermentation in heifer later in life, the dietary experience after transition to same TMR would eventually override the effect of early feed experience.


1997 ◽  
Vol 37 (5) ◽  
pp. 505 ◽  
Author(s):  
W. K. Ehrlich ◽  
T. M. Davison

Summary. Twenty-four Holstein–Friesian cows were used in an experiment comparing milk production from cows offered rolled sorghum grain either alone at 8 or 10 kg/head. day of air-dried grain or with 4% sodium bentonite. The design was a 4 × 4 latin square with a 1 week adjustment period and a 3 week treatment period. This design was used to highlight the effects of high levels of grain feeding and changing that level of grain or grain–bentonite every 4 weeks. Cows grazed either ryegrass (Lolium multiflorum cv. Tetila) or oats (Avena sativa cv. Cluan) during the day and a mixed ration based on maize silage, lucerne hay, and meat and bone meal at night. There was no significant effect of treatments on milk yield or composition. Cows fed bentonite had a higher (P<0.05) rumen pH, tended to eat less grain sorghum and have lower concentrations of rumen ammonia and faecal starch. Faecal crude protein tended to increase with the use of bentonite indicating cows may have substituted pasture or mixed ration for grain and maintained a more stable rumen fermentation.


Author(s):  
S. Biswal ◽  
G. S. Parida ◽  
A. K. Biswal ◽  
K. K. Sardar ◽  
P. C. Bisoi ◽  
...  

The aim of this study was to investigate the prevalence and therapeutic management of indigestion in milch cows in and around Bhubaneswar, Odisha. Crossbred milch cows (n=257) with the history of anorexia presented to Teaching Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar and in private herds in and around Bhubaneswar were examined for indigestion following standard procedures to identify the type of indigestion leading to decrease in milk yield. Clinical examination, determination of pH, study of rumen protozoa, rumen bacteria were carried out in three different types of indigestion. Amongst the affected animals, 48.33 % (n=58), 21.67 % (n=26) and 30.00 % (n=36) were detected to be affected with simple, acid and alkaline indigestion, respectively. Simple indigestion was higher (p < 0.05) as compared to acid and alkaline and alkaline indigestion (30 %) was found to be the next to simple indigestion. Ruminal movement varied from 1-4 per 5 min and pH of the rumen fluid ranged from 7.5-8.6 and the idophillic activity of the rumen protozoa was moderate type (+++). Average total count of protozoa was estimated to be 3.5 - 4.6 x 105 per ml with an average of 4.1 x 105 per ml of rumen liquor and drugs used in the study were found efficacious to treat the different types of indigestion.. Rumen bacteria have played role resulting in production of volatile fatty acids and lactic acid thereby decreasing / disturbing the rumen pH to non-physiological levels, reducing efficiency of rumen microflora.


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