Prevalence and therapeutic management of indigestion in milchcows in and around Bhubaneswar of Odisha, India

2016 ◽  
Author(s):  
S. Biswal ◽  
G. S. Parida ◽  
A. K. Biswal ◽  
K. K. Sardar ◽  
P. C. Bisoi ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Animal Husbandry ◽  
Therapeutic Management ◽  
Rumen Bacteria ◽  
Rumen Protozoa ◽  
Rumen Ph ◽  
Standard Procedures ◽  
Different Types ◽  
History Of

The aim of this study was to investigate the prevalence and therapeutic management of indigestion in milch cows in and around Bhubaneswar, Odisha. Crossbred milch cows (n=257) with the history of anorexia presented to Teaching Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar and in private herds in and around Bhubaneswar were examined for indigestion following standard procedures to identify the type of indigestion leading to decrease in milk yield. Clinical examination, determination of pH, study of rumen protozoa, rumen bacteria were carried out in three different types of indigestion. Amongst the affected animals, 48.33 % (n=58), 21.67 % (n=26) and 30.00 % (n=36) were detected to be affected with simple, acid and alkaline indigestion, respectively. Simple indigestion was higher (p < 0.05) as compared to acid and alkaline and alkaline indigestion (30 %) was found to be the next to simple indigestion. Ruminal movement varied from 1-4 per 5 min and pH of the rumen fluid ranged from 7.5-8.6 and the idophillic activity of the rumen protozoa was moderate type (+++). Average total count of protozoa was estimated to be 3.5 - 4.6 x 105 per ml with an average of 4.1 x 105 per ml of rumen liquor and drugs used in the study were found efficacious to treat the different types of indigestion.. Rumen bacteria have played role resulting in production of volatile fatty acids and lactic acid thereby decreasing / disturbing the rumen pH to non-physiological levels, reducing efficiency of rumen microflora.

Isolation and identification of rumen bacteria capable of anaerobic rutin degradation

1969 ◽  
Vol 15 (12) ◽  
pp. 1365-1371 ◽  
Author(s):  
K. -J. Cheng ◽  
G. A. Jones ◽  
F. J. Simpson ◽  
M. P. Bryant
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Anaerobic Bacteria ◽  
Heterocyclic Ring ◽  
Ring Structure ◽  
Water Soluble ◽  
Rumen Bacteria ◽  
Isolation And Identification ◽  
Pure Cultures ◽  
Rutin Degradation

Fifteen strains of bacteria capable of degrading rutin anaerobically were isolated from bovine rumen contents and identified by morphological and biochemical evidence as strains of Butyrivibrio sp. Three cultures from a laboratory collection of 53 strains of rumen bacteria also used rutin anaerobically. Two, Butyrivibrio fibrisolvens D1 and Selenomonas ruminantium GA192, cleaved the glycosidic bond of rutin and fermented the sugar but did not degrade the insoluble aglycone produced; the third strain, Peptostreptococcus sp. B178, degraded the substrate to soluble products. Butyrivibrio sp. C3 degraded rutin, quercitrin, and naringin to water-soluble products, showing that the organism cleaved the heterocyclic ring of these compounds. Butyrivibrio sp. C3 fermented the sugar moiety of hesperidin but did not cleave the heterocyclic ring. It did not attack quercetin, taxifolin, protocatechuic acid, or phloroglucinol. In a medium containing rumen fluid, Butyrivibrio sp. C3 degraded rutin more than twice as fast as it did in a medium containing enzymatic casein hydrolyzate, volatile fatty acids, yeast extract, and hemin in place of rumen fluid.The observations reported in this paper are believed to represent the first recorded demonstration of degradation of the heterocyclic ring structure of rutin and other bioflavonoids in pure cultures of anaerobic bacteria.

scholarly journals Observations on the microbiology and biochemistry of the rumen in cattle given different quantities of a pelleted barley ration

1970 ◽  
Vol 24 (1) ◽  
pp. 157-177 ◽  
Author(s):  
J. Margaret Eadie ◽  
J. Hyldgaard-Jensen ◽  
S. O. Mann ◽  
R. S. Reid ◽  
F. G. Whitelaw
Keyword(s):  
Propionic Acid ◽  
Butyric Acid ◽  
Volatile Fatty Acid ◽  
Bacterial Population ◽  
Microbial Population ◽  
Rumen Fluid ◽  
Complete Loss ◽  
Rumen Bacteria ◽  
Diurnal Pattern ◽  
Rumen Ph

1. Three heifers were changed from a diet of equal parts of hay and barley cubes (50:50 diet) to one entirely of barley cubes given in three equal feeds throughout the day. Feed intake was restricted to 80% of calculated appetite at the time of change and this percentage progressively decreased as the live weights of the animals increased.2. The change of diet had no significant effect on the volume of rumen fluid but the rate of outflow from the rumen was significantly lower on the barley diet than on the 50:50 diet.3. Animals on the restricted barley diet developed an exceptionally high rumen ciliate population and the bacterial population was shown by Gram films to include a number of organisms typical of roughage-fed animals. In culture, organisms of the genusBacteroideswere predominant but these appeared largely as cocco-bacilli in the Gram films. This microbial population was associated with a higher proportion of butyric acid than of propionic acid in the rumen fluid.4. Occasional fluctuations in ciliate populations occurred in all three heifers. Decreases in ciliate number were paralleled by increases in propionic acid and decreases in butyric acid but not necessarily by a fall in pH. Under these conditions Gram films showed increases in bacteriodes-type rods and in certain curved Gram-negative rods.5. Rumen ammonia concentrations were on average lower and showed a different diurnal pattern when ciliate numbers were reduced. Lactic acid concentrations were low and were not affected by the size of the ciliate population.6. When the three heifers were given the barley dietad lib. there was a decrease in rumen pH and a complete loss of rumen ciliates. The rumen bacterial population and the volatile fatty acid proportions were similar to those seen during decreases in ciliate number at the restricted level of intake. These changes also occurred in a fourth heifer which was changed fairly rapidly from the 50:50 diet to a restricted amount of the barley diet.7. Two steers which had never had access to roughage were changed fromad lib. to restricted intake of the barley diet and were later given an inoculum of rumen ciliates. The rumen microbial population and the pattern of fermentation so produced were similar to those found in the heifers on the restricted barley diet.8. Anomalous values were noted for total counts of rumen bacteria when free starch grains were present in the rumen fluid.9. It is concluded that large ciliate populations and high proportions of butyric acid can be produced in animals fed exclusively on a barley diet by suitable adjustment of the intake and the method of feeding. It is postulated that the ciliate population may be largely responsible for the high butyric acid concentrations.

scholarly journals Duodenal flow and digestibility in fauna-free sheep and in sheep monofaunated with Entodinium caudatum or Polyplastron multivesiculatum

2006 ◽  
Vol 95 (3) ◽  
pp. 469-476 ◽  
Author(s):  
M. Ivan ◽  
K.M. Koenig ◽  
D.P. Morgavi ◽  
L.M. Rode ◽  
C.J. Newbold ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Dual Phase ◽  
Rumen Bacteria ◽  
Microbial Protein ◽  
Total Volatile ◽  
Equal Degree ◽  
Faecal Samples ◽  
Acid Detergent Fibre ◽  
Protozoan Species

Three groups of five rumen and duodenum cannulated fauna-free sheep were used in a 28d experiment. One group remained fauna-free, whereas the second (EN) and third (PP) groups, respectively, were inoculated intraruminally with the protozoan species Entodinium caudatum and Polyplastron multivesiculatum. Rumen fluid, duodenal digesta and faecal samples were collected during the last 12d. The flow of digesta to the duodenum was determined using Yb and Co as dual-phase markers. 15Nitrogen and phosphatidylcholine were used as markers to calculate the duodenal flow of bacterial and protozoal N, respectively. Results showed an increase (P<0·1) in the rumen concentration of NH3-N and total volatile fatty acids, and a decrease (P<0·05) in the duodenal flow of non-NH3-N and bacterial N in sheep with EN and PP monofaunas, compared with fauna-free sheep. There were no differences (P>0·05) in these variables between the two monofauna groups. Protozoal N accounted for 8% of the duodenal non-NH3-N flow in the EN-monofaunated sheep, whereas no such flow was detected in the PP-monofaunated sheep. Apparent rumen digestibility of organic matter, neutral detergent fibre and acid detergent fibre were similar (P>0·05) in the monofaunated groups of sheep, but rumen acid detergent fibre digestibility was higher (P<0·05) in the monofaunated than in the fauna-free groups. Experimental results suggested that, unlike EN, the PP monofauna might not contribute to the duodenal flow of microbial protein, whereas both monofaunas showed a virtually equal degree of predation on rumen bacteria.

scholarly journals Some effects of rumen ciliate protozoa in cattle given restricted amounts of a barley diet

1972 ◽  
Vol 27 (2) ◽  
pp. 425-437 ◽  
Author(s):  
F. G. Whitelaw ◽  
J. Margaret Eadie ◽  
S. O. Mann ◽  
R. S. Reid
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Initial Period ◽  
Rumen Ph ◽  
Large Populations ◽  
Blood Haemoglobin ◽  
Daily Allowance ◽  
Spontaneous Fluctuations ◽  
Amino Acid Concentrations ◽  
Ciliate Protozoa

1. Two pairs of Friesian steers were changed fromad lib.to restricted intake of a pelleted barley diet and were maintained on this latter intake for periods of 18 or 25 weeks. The restricted level of intake was 70 g/kg0.73and was adjusted weekly according to individual live weights. The daily allowance was given in three equal feeds during day-time.2. After an initial period of 9 weeks on the restricted diet, during which all four animals were kept free of rumen ciliate protozoa, one member of each pair was given an inoculum of rumen ciliates. Eight weeks later, the ciliate-free member of the younger pair of steers was similarly inoculated. Observations were made on the rumen bacterial and protozoal populations and on changes in rumen pH and volatile fatty acids (VFA) throughout each treatment period. The concentrations of urea and haemoglobin in blood and of glucose and amino acids in plasma were examined on one occasion in each animal.3. In the absence of ciliates, restriction of intake resulted in rumen pH values and molar proportions of VFA similar to those normally encountered on anad lib.intake of a barley diet. A decrease in bacterial numbers and certain minor changes in bacterial types were observed on changing fromad lib.to restricted intake but the resultant population under ciliate-free conditions was basically the same as that found later in the faunated animals. In culture, organisms of the genusBacteroideswere predominant.4. Large populations of rumen ciliates were established in each animal inoculated. Relative to the ciliate-free periods, the presence of ciliates resulted in an increase in rumen pH, a reduction in total VFA concentration and a decrease in the ratio of propionic to butyric acid in rumen fluid. It is concluded that these changes are a direct effect of ciliate activity.5. Conditions within the rumen remained more stable from day to day when large ciliate populations were present than when ciliates were absent. In one animal, spontaneous fluctuations in ciliate number were accompanied by corresponding changes in rumen pH and VFA proportions.6. Significant differences were observed between faunated and ciliate-free animals in the concentration urea in blood and of glucose in plasma; only minor differences were noted in blood haemoglobin and plasma amino acid concentrations.

scholarly journals The effect of phosphate and carbonate-bicarbonate supplements on the rumen buffering systems of sheep

1970 ◽  
Vol 24 (3) ◽  
pp. 653-660 ◽  
Author(s):  
Bijan Emmanuel ◽  
M. J. Lawlor ◽  
D. McAleese
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Control Diet ◽  
Cellulase Activity ◽  
Inorganic Phosphorus ◽  
Mineral Supplement ◽  
Mineral Supplements ◽  
Ph Values ◽  
Rumen Ph ◽  
Ph Range

1. Two wethers fitted with permanent rumen cannulas were used to study the effect of two mineral supplements, Na2HPO4 and a 1:1 mixture of Na2CO3 and NaHCO3, on the buffering system in the rumen of sheep given a pelleted roughage-concentrate diet.2. Measurements were made of rumen pH, buffering capacity value (β), total volatile fatty acids (VFA), CO2 and inorganic phosphorus concentrations as well as rumen cellulase activity.3. The addition of either mineral supplement to the diet significantly (P < 0.001) increased the rumen pH values during the critical post-feeding period, there was a difference of only 0.5 pH unit between the minimum and maximum pH values.4. Both mineral supplements significantly increased the concentrations of CO2 in the rumen fluid and rumen cellulase activity. They resulted in a general reduction in total VFA concentrations. It is suggested that this may possibly arise from increased absorption of VFA associated with increased CO2 tension in the rumen fluid.5. The Na2HPO4 supplement significantly ( P < 0.001) increased the β values in the pH range 6–8, whereas the bicarbonate supplement contributed to raising the β values in the pH range 5–6.6. The bicarbonate supplements raised cellulose digestibility from 39.7% for the control diet to 45.6% and the addition of Na2HPO4 further raised the digestibility to 51.1%. It would appear that low rumen pH impairs rumen cellulase activity and this could be offset by the addition of mineral supplements.

scholarly journals Providing calves different feed source early in life would not affect feed sorting and rumen fermentation for a long term

2020 ◽  
Author(s):  
Jianxin Xiao ◽  
Muhammad Zahoor Khan ◽  
Gibson Maswayi Alugongo ◽  
Wei Wang ◽  
Yajing Wang ◽  
...  
Keyword(s):  
Fine Particle ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Fine Particle Fraction ◽  
Particle Fraction ◽  
Calf Health ◽  
Rumen Ph ◽  
Short Period

Abstract This study aimed to investigate the short and long-term effect of early in life exposure to different feed sources on feed sorting and rumen fermentation of calves. Forty newborn female Holstein calves were randomly divided based on supplementation of concentrate (CON) or hay (HAY) only during the milk-feeding stage (d 1 to 56). After that, all calves were offered a total mixed ration (TMR) containing 43% of forage and 57% of concentrate until the end of the experiment (d 57 to 196). Calves were weighed immediately after birth and raised in individual hutches until d 70. The calves were then transferred to a heifer barn, housed within treatment in pairs (2 calves/pen). Rectal temperature, feed intake, body weight, body structural measurement, fecal and cough score were recorded ranges from day 1 to 196. Fresh and orts feed were sampled daily at the first two weeks (d 57-70) once TMR was offered and at the last week of the experiment (d 190 to 196) for analysis of feed sorting. Rumen fluid samples were collected at ranges from d 14-190 for detecting rumen pH and volatile fatty acids (VFA) concentrations. Our findings showed that early feed exposure did not influence calf health and growth performance but affected feed sorting in a short period. Upon transition to the same mixed diet, differences were found between treatments in the sorting of the different particle fractions. At week 9 and 10, calves early exposed to concentrates began to sort for fine particle fraction (primarily concentrate in this fraction), and against the long particle fraction (solely forage in this fraction), whereas calves early fed hay sorted for the long particle fraction, and against the fine particle fraction. However, no carryover effect was found, the differences between treatments fade away when detecting feed sorting at the last week, all calves sorted for short and fine particle fractions, and sort against long and medium particle fractions. Although the pattern of feed sorting initially differed between treatments, the rumen pH and VFA concentration were not altered once all calves transitioned to a TMR. These results suggested that the feed familiarity established early in life would not affect diet selection and rumen fermentation in heifer later in life, the dietary experience after transition to same TMR would eventually override the effect of early feed experience.

scholarly journals Providing calves different feed source early in life would not affect feed sorting and rumen fermentation for a long term

2020 ◽  
Author(s):  
Jianxin Xiao ◽  
Muhammad Zahoor Khan ◽  
Gibson Maswayi Alugongo ◽  
Wei Wang ◽  
Yajing Wang ◽  
...  
Keyword(s):  
Fine Particle ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Fine Particle Fraction ◽  
Particle Fraction ◽  
Calf Health ◽  
Rumen Ph ◽  
Short Period

Abstract This study aimed to investigate the short and long-term effect of early in life exposure to different feed sources on feed sorting and rumen fermentation of calves. Forty newborn female Holstein calves were randomly divided based on supplementation of concentrate (CON) or hay (HAY) only during the milk-feeding stage (d 1 to 56). After that, all calves were offered a total mixed ration (TMR) containing 43 % of forage and 57 % of concentrate until the end of the experiment (d 57 to 196). Rectal temperature, feed intake, body weight, body structural measurement, fecal and cough score were recorded ranges from day 1 to 196. Fresh and orts feed were sampled daily at the first two weeks (d 57-70) once TMR was offered and at the last week of the experiment (d 190 to 196) for analysis of feed sorting. Rumen fluid samples were collected at ranges from d 14-190 for detecting rumen pH and volatile fatty acids (VFA) concentrations. Our findings showed that early feed exposure did not influence calf health and growth performance but affected feed sorting in a short period. Upon transition to the same mixed diet, differences were found between treatments in the sorting of the different particle fractions. At week 9 and 10, calves early exposed to concentrates began to sort for fine particle fraction, and against the long particle fraction, whereas calves early fed hay sorted for the long particle fraction, and against the fine particle fraction. However, no carryover effect was found, the differences between treatments fade away when detecting feed sorting at the last week, all calves sorted for short and fine particle fractions, and sort against long and medium particle fractions. Although the pattern of feed sorting initially differed between treatments, the rumen pH and VFA concentration were not altered once all calves transitioned to a TMR. These results suggested that the feed familiarity established early in life would not affect diet selection and rumen fermentation in heifer later in life, the dietary experience after transition to same TMR would eventually override the effect of early feed experience.

scholarly journals Comparison of grass silage utilization by reindeer and sheep: 2. Rumen fermentation and rumen microbiota

1982 ◽  
Vol 54 (2) ◽  
pp. 127-135
Author(s):  
Liisa Syrjälä-Qvist
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Rumen Microbiota ◽  
Grass Silage ◽  
Rumen Ph ◽  
Sampling Times

The criteria used in comparing the utilization of grass silage by reindeer and sheep were rumen pH, ammonia, volatile fatty acids (VFA) and microbes. Rumen samples were taken before feeding, and 2 ½ and 5 ½ hours after the beginning of feeding. Rumen fermentation was lower in the reindeer than in the sheep and differed less between the three sampling times. In the reindeer/the pH of the rumen fluid averaged 6.94 and in the sheep 6.61. The average amounts of NH3—N were 17.0 and 24.2 mg/100 ml rumen fluid and those of total VFA 8.46 and 10.90 mmoles/100ml rumen fluid, respectively. The proportion of acetic acid in the VFA in the reindeer was 75.3 molar % and in the sheep 66.0 molar %, the corresponding values for propionic acid being 18.5 and 22.0 molar % and for butytic acid 4.2 and 8.8 molar %. The number of rumen ciliates in the reindeer averaged 87/mm3 rumen contents and in the sheep 314/ mm3. The numbers of bacteria were 16.0 X 106/mm3, respectively. The proportion of the total microbe mass in the reindeer rumen contents was 1.8 % and in the sheep 2.4 %. The proportions of bacteria in this mass were 87 % and 70 %, respectively. The differences between the reindeer and sheep in the rumen fermentation results and in the numbers of rumen microbiota were nearly all statistically significant (P

DIGESTIVE PARAMETERS OF PUREBREED SHEEP AND THEIR HYBRIDS WITH ARGALI

2016 ◽  
Vol 1 (1) ◽  
pp. 44-46
Author(s):  
Девяткин ◽  
Vladimir Devyatkin ◽  
Романов ◽  
Viktor Romanov ◽  
Боголюбова ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Animal Husbandry ◽  
Ammonia Concentration ◽  
Domestic Sheep ◽  
The Third ◽  
Group I ◽  
Comparative Aspect ◽  
The Comparative Study ◽  
Digestive Processes

The aim of researches is to develop physiological bases of sheep productivity through improved adaptive capacity of the organism. In comparative aspect the peculiarities of the digestive processes in sheep of different genotypes: the domestic sheep and their hybrids with argali. The experiments were carried out in L. K. Ernst Institute of Animal Husbandry in tree groups of sheep of different origins (n=3) with a rumen fistula. Animals of group I – sheep-valukh the Romanov breed II – the hybrids of the second generation ewes of the Romanov breed with argali (25% of kinship) and III – hybrids of the third generation of Edilbaj breed (50% of kinship), of the Romanov breed (37.5% of kinship) and argali (12.5% of kinship). Higher feed intake was observed in hybrid animals. So, consumption of dry matter in the second group it was higher by 14.6%, the third with 15.4%, compared to the control, obviously, was determined breed differences in the intensity of the digestive processes. Set that the origin of the animals affects the orientation of enzymatic processes. In the proventriculus hybrid animals it was more intense compared with the number purebred. This is evidenced by a statistically significant increase in hybrid animals of the concentration of volatile fatty acids (12-21.6% to nursing and 8.7-12% after nursing), a significant increase of amylolytic activity of the rumen fluid (1.5-2.04 E/ml), some reduction in the ammonia concentration. Had higher content of total quantity of microbial mass in the hybrids of the Romanov sheep and argali compared to purebred sheep-valukh (227.1-258.0 mg/100 ml). Of further interest is the comparative study of digestive and metabolic processes at hybrid animals for the op for determining the optimal type and structure of diets.

Effects of forage neutral detergent fibre and time after feeding on medial and ventral rumen pH and volatile fatty acids concentration in heifers fed highly digestible grass/clover silages

2017 ◽  
Vol 57 (1) ◽  
pp. 129 ◽  
Author(s):  
A. K. S. Schulze ◽  
A. C. Storm ◽  
M. R. Weisbjerg ◽  
P. Nørgaard
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Growth Stages ◽  
Neutral Detergent Fibre ◽  
Rumen Epithelium ◽  
Rumen Ph

The major microbial fermentation of forages and production of volatile fatty acids (VFA) takes place in the medial part of the rumen, whereas the absorption of VFA occurs through the rumen epithelium, for example the ventral sac. The objective was to study effects of forage neutral detergent fibre (NDF) content and time after feeding on the medial to ventral VFA and pH gradient as well as rumen motility in the rumen of heifers fed grass/clover silages. Four silages were harvested at different growth stages with NDF contents of 31–45% of DM and in vitro organic matter digestibilities of 75–82% and fed to four rumen-fistulated Jersey heifers at 90% of ad libitum level in a Latin square design, with half the ration fed at 0800 hours and 1530 hours. Rumen fluid was sampled hourly from 0730 hours to 1530 hours in the medial and ventral rumen, and analysed for pH and concentrations of VFA, L-lactic acid, and ammonia to assess ruminal chemical gradient. Reticular contractions were continuously recorded by a pressure transducer. Time relative to feeding affected rumen parameters as pH was generally lower and VFA content greater in medial compared with ventral rumen fluid. Greater NDF content of the silage caused lower VFA concentration and higher pH in the rumen mat, and therefore the gradient diminished at greater NDF content in the silages; an effect probably caused by reduced organic matter digestibility rather than digesta NDF properties. This study therefore suggests that VFA production decreased with greater NDF content of forages, whereas intra-ruminal equilibration increased.

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