scholarly journals Compare the efficiency of colposcopic VIA, VILI, LIQUIPREP TM and conventional Pap smear; as screening procedure for carcinoma cervix

2018 ◽  
Vol 8 (1) ◽  
pp. 69
Author(s):  
Nithya Subbaiyan ◽  
Sabari Kasinathan
Keyword(s):  
Sensitivity And Specificity ◽  
Pap Smear ◽  
Screening Programme ◽  
Reproductive Age ◽  
Screening Procedure ◽  
High Grade ◽  
Cervical Biopsy ◽  
High Grade Lesion ◽  
Cancer Of The Cervix ◽  
Histopathological Studies

Background: Cancer of the cervix is a leading cause of morbidity and mortality among women worldwide. Therefore, to curb the disease, there is a need to develop a screening test that has good sensitivity and specificity. The aim is to compare the efficiency of Colposcopic Via, Vili, Liquiprep TM and conventional pap smear; as screening procedure for carcinoma cervix.Methods: This study was conducted in 100 women in the reproductive age. The pap smear and VIA are done in these cases. In positive cases, cervical biopsy and histopathological studies are done, the sensitivity and specificity of each test are determined and compared.Results: In this study, more cases belonged to 30 - 45 years age group. Among 100 women, 15% cases had high-grade lesion. Among high-grade lesions group, one case that is 6.7% had CIN 1, 73.3% had CIN2/3 and 3cases i.e., 20% had cervicitis. Among 100 women, 1% had ASCUS result, 10% belongs to HSIL group, 39% belongs to LSIL group and 2% had an unsatisfactory result.Conclusions: The lack of an effective and implementable screening programme lead to reporting of advanced cases of Ca Cervix. If detected at CIN or early Ca cervix stage, effective treatment can be provided with encouraging results. Therefore, effective and implementable Ca Cervix screening need to be provided in our country.

Comparison of efficacy of pap smear cytology with lugol’s iodine guided cervical biopsy in evaluation of unhealthy cervix

2017 ◽  
Author(s):  
Indira Surya Kumari Kothapalli ◽  
Sindhuja Dogga
Keyword(s):  
Sensitivity And Specificity ◽  
Visual Inspection ◽  
Pap Smear ◽  
Punch Biopsy ◽  
Cancer Cervix ◽  
Lugol’S Iodine ◽  
Cervical Biopsy ◽  
Pap Smear Screening ◽  
Malignant Lesions ◽  
Sensitivity Specificity

Background: Carcinoma cervix is the 3rd most common cancer in the world. In India alone, 130000 new cases occur with the death toll of 70000 cases every year. This study is done to compare the efficacy of pap smear cytology with lugol’s iodine guided cervical biopsy in detecting pre-malignant and malignant lesions of unhealthy cervix.Methods: The study comprises of 100 women aged between 18-65 years with clinically unhealthy cervix. They were subjected to pap smear, cervical punch biopsy, followed by visual inspection with lugol’s iodine (VILI). Sensitivity, specificity, positive predictive value and accuracy were calculated for pap smear and VILI using cervical biopsy as the standard diagnostic test.Results: Pap smear revealed that 64% had inflammatory smear and 17% had positive pap smear. On visual inspection of cervix with lugol’s iodine, 19 cases (19%) were VILI positive and 81 cases were VILI negative. Among 100 cases, 19 cases were biopsy positive and 81 cases were biopsy negative. Sensitivity of pap smear is 78.95% and specificity is 97.53%. Sensitivity of VILI is 94.74 and specificity is 98.77. For detecting all grades of CIN, VILI had an accuracy of 98%.Conclusions: Visual inspection with lugol’s iodine is more sensitive and accurate than pap smear. Screening of cancer cervix in low resource settings could be carried out by visual inspection of Lugol’s iodine (VILI) as it has good sensitivity and specificity. By combining pap smear with VILI and cervical biopsy, we can further maximize the sensitivity and specificity of cancer cervix screening.

A comparison of cytology and cervicography in cervical screening

1993 ◽  
Vol 3 (6) ◽  
pp. 395-398 ◽  
Author(s):  
V. I. Kesic ◽  
W. P. Soutter ◽  
V. Sulovic ◽  
N. Juznic ◽  
M. Aleksic ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Screening Programme ◽  
Cervical Screening ◽  
Invasive Cancer ◽  
Cervical Cytology ◽  
Screening Methods ◽  
Cin Iii ◽  
The Difference ◽  
Cancer Of The Cervix

The sensitivity and specificity of cervical cytology and of cervicography in the detection of CIN and invasive cancer of the cervix were determined in a screening programme of an asymptomatic population of 418 women. Because all of the subjects were examined colposcopically and biopsies were taken from any suspicious areas, the presence or absence of disease was determined by the histology of directed cervical biopsies independently of the screening methods being tested. Cervicography correctly identified 24 of the 27 women with CIN or invasive cancer, whereas cytology detected only 14 (sensitivity of 0.89 vs. 0.52;P< 0.01). Cervicography detected CIN in 11 women with negative smears, six having CIN III. Only one case of CIN I was detected by cytology when cervicography was negative. Two cases of CIN II were not detected by either method. Inclusion in the analysis of the results from 23 women with technically defective cervigrams reduced the size of the difference in sensitivity, but it remained significant (0.74 vs. 0.48;P< 0.05). Cervicography was only slightly less specific than cytology (0.92 vs. 0.94; NS). Cervicography appears to be more effective than cytology in screening for cervical premalignancy but may be more demanding of those who perform the test.

scholarly journals Multiple HPV 16 infection with two strains: a possible marker of neoplastic progression

2020 ◽  
Author(s):  
Maria Teresa Bruno ◽  
Guido Scalia ◽  
Nazario Cassaro ◽  
Sara Boemi
Keyword(s):  
Hpv Infection ◽  
Multiple Infections ◽  
High Grade ◽  
Neoplastic Progression ◽  
Cervical Biopsy ◽  
High Grade Lesion ◽  
Neoplastic Lesions ◽  
Clinically Significant ◽  
Hpv16 Infection ◽  
Common Infection

Abstract Background. We studied the cases of single and multiple HPV infection and analyzed the correlation with negative cases, and preneoplastic and neoplastic lesions of the uterine cervix with the aim of making a contribution to the prognostic factor under discussionMethods: 909 women undergoing second level screening because they had been positive at cervical cytology were enrolled. All the patients underwent colposcopy and cervical biopsy with viral genotyping. We divided mHPV infection based on the number of genotypes present: infections with 2 strains, 3 strains, 4 strains and 5 or more strains.Statistical analysis The analysis of the data was made using the χ2 test. Contingency tables were created to evaluate the correlation between single, multiple and CIN2+ infections. Values with p <0.05 were considered statistically significant.Results: The presence of genotype HPV16 in our study was associated with a 12 times greater risk of developing a high-grade lesion, OR = 12.70. The patients with single infections had the highest incidence of CIN2+ (34.1 %) with respect to those with multiple infections (10.6%).When we studied in the mHPV infection the prevalence of the combinations between the genotypes, we found that in mHPV16 infections, the combinations HPV16, 18 and HPV16, 31 were the most frequent (55.5%) in CIN3 lesion.ConclusionsOur results suggest that single HPV infections have a greater risk of developing SCC with respect to multiple infections. Multiple HPV infections are relevant only in the first phase of the lesion (CIN1-CIN2), while they are absent in carcinomas, where infections are of a single genotype. In particular, among multiple infections, HPV16 infection with 2 HR genotypes is associated significantly with CIN2 / CIN3 (21/30) and has 4 times greater risk of developing a high-grade lesion. Thus, it is probable that only specific combinations of HPV (HPV16,18 - HPV 16,31) can be associated with a clinically significant impact, while other combinations can simply be correlated because of a common infection or diagnostic method used. Therefore, multiple HPV16 infections with two high-risk genotypes is a major risk of CIN2/CIN3.

scholarly journals Multiple HPV 16 infection with two strains: a possible marker of neoplastic progression

2020 ◽  
Author(s):  
Maria Teresa Bruno ◽  
Guido Scalia ◽  
Nazario Cassaro ◽  
Sara Boemi
Keyword(s):  
Hpv Infection ◽  
Multiple Infections ◽  
High Grade ◽  
Neoplastic Progression ◽  
Cervical Biopsy ◽  
High Grade Lesion ◽  
Neoplastic Lesions ◽  
Clinically Significant ◽  
Hpv16 Infection ◽  
Common Infection

Abstract Background. We studied the cases of single and multiple HPV infection and analyzed the correlation with negative cases, and preneoplastic and neoplastic lesions of the uterine cervix with the aim of making a contribution to the prognostic factor under discussionMethods: 909 women undergoing second level screening because they had been positive at cervical cytology were enrolled. All the patients underwent colposcopy and cervical biopsy with viral genotyping. We divided mHPV infection based on the number of genotypes present: infections with 2 strains, 3 strains, 4 strains and 5 or more strains. Statistical analysis The analysis of the data was made using the χ2 test. Contingency tables were created to evaluate the correlation between single, multiple and CIN2+ infections. Values with p <0.05 were considered statistically significant.Results: The presence of genotype HPV16 in our study was associated with a 12 times greater risk of developing a high-grade lesion, OR = 12.70. The patients with single infections had the highest incidence of CIN2+ (34.1 %) with respect to those with multiple infections (10.6%).When we studied in the mHPV infection the prevalence of the combinations between the genotypes, we found that in mHPV16 infections, the combinations HPV16, 18 and HPV16, 31 were the most frequent (55.5%) in CIN3 lesion.ConclusionsOur results suggest that single HPV infections have a greater risk of developing SCC with respect to multiple infections. Multiple HPV infections are relevant only in the first phase of the lesion (CIN1-CIN2), while they are absent in carcinomas, where infections are of a single genotype. In particular, among multiple infections, HPV16 infection with 2 HR genotypes is associated significantly with CIN2 / CIN3 (21/30) and has 4 times greater risk of developing a high-grade lesion. Thus, it is probable that only specific combinations of HPV (HPV16,18 - HPV 16,31) can be associated with a clinically significant impact, while other combinations can simply be correlated because of a common infection or diagnostic method used. Therefore, multiple HPV16 infections with two high-risk genotypes is a major risk of CIN2/CIN3.

scholarly journals p16/Ki-67 dual staining has a better accuracy than human papillomavirus (HPV) testing in women under 30 years old

2018 ◽  
Author(s):  
Laurențiu Pirtea ◽  
Cristina Secosan ◽  
Madalin Margan ◽  
Lavinia Moleriu ◽  
Oana Balint ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Sensitivity And Specificity ◽  
Pap Smear ◽  
High Rate ◽  
Low Grade ◽  
High Grade ◽  
Cervical Lesions ◽  
Ki 67 ◽  
Hpv Genotyping ◽  
Dual Staining

Due to a high rate of transient human papillomavirus (HPV) infection, HPV genotyping has a low specificity for high-grade cervical lesions, especially in young women. p16/Ki-67 dual immunohistochemical staining can also be used for the detection of oncogenic changes in cervical cells. Our aim was to compare the performance of p16/Ki-67 dual staining and HPV genotyping in the detection of high-grade cervical lesions in patients with atypical squamous cells of undetermined significance (ASCUS)/low-grade squamous intraepithelial lesion (LSIL) on Pap smear. We retrospectively analyzed 310 patients with ASCUS/LSIL on Pap smear, who underwent colposcopy. Among these, 161 patients with suspected lesions detected by colposcopy were referred to biopsy. HPV genotyping by LINEAR ARRAY HPV Genotyping Test (CE-IVD) and p16/Ki-67 dual staining by CINtec PLUS Cytology kit was performed prior to cervical biopsy. The overall sensitivity and specificity of HPV genotyping for the detection of cervical intraepithelial neoplasia (CIN) 2-3 was 79% and 72%, respectively in patients with ASCUS, and 85% and 64%, respectively in patients with LSIL. For p16/ki-67 test, sensitivity and specificity rate was 66% and 93%, respectively in ASCUS and 59% and 79%, respectively in LSIL group. The specificity of p16/Ki-67 staining was significantly higher in both groups in patients aged <30 years compared to patients >30 years old (p < 0.001). Our results showed that p16/Ki-67 dual staining has a higher specificity compared to HPV genotyping, especially in patients under 30 years old. This indicates the usefulness of p16/Ki-67 testing in the triage of patients with ASCUS/LSIL and <30 years old, prior to the referral to colposcopy and biopsy.

scholarly journals Loop Electrosurgical Excisional Procedure (LEEP) Done for Discrepancy: Does the Time from HGSIL Affect Pathologic Grade of CIN in LEEP Specimen?

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Sue L. Moreni ◽  
Caroline M. Mitchell ◽  
Rochelle L. Garcia ◽  
Linda O. Eckert
Keyword(s):  
Study Design ◽  
Pap Smear ◽  
Case Control Study ◽  
Case Control ◽  
Low Grade ◽  
High Grade ◽  
Retrospective Case ◽  
Cervical Biopsy ◽  
Management Alternative ◽  
Control Study

Objective. When pathologic discrepancy arises between high-grade cytology on Papanicolaou (Pap) smear and low-grade histology on cervical biopsy, Loop Electrosurgical Excisional Procedure (LEEP) is one management alternative. Our objective was to determine whether the time from initial HGSIL Pap to LEEP affects the pathologic grade of the LEEP specimen.Study Design. We performed a retrospective case-control study identifying LEEPs performed for discrepancy over a 10-year period (1997–2007). 121 subjects were separated into two groups based on LEEP pathology (≤CIN1 and CIN 2,3) and compared usingχ2.Results. Of the 121 LEEP specimens, 67 (55.4%) had CIN 2,3. CIN 2,3 was more often discovered when LEEP was performed within 3 months of the HGSIL Pap smear versus after 5 months (55.2% versus 16.4%,P=.096).Conclusion. Women undergoing LEEP for discrepancy >5 months from their HGSIL Pap demonstrated a trend toward less CIN 2,3 on LEEP pathology.

scholarly journals Multiple HPV 16 infection with two strains: a possible marker of neoplastic progression

2020 ◽  
Author(s):  
Maria Teresa Bruno ◽  
Guido Scalia ◽  
Nazario Cassaro ◽  
Sara Boemi
Keyword(s):  
Hpv Infection ◽  
Multiple Infections ◽  
High Grade ◽  
Neoplastic Progression ◽  
Cervical Biopsy ◽  
High Grade Lesion ◽  
Neoplastic Lesions ◽  
Clinically Significant ◽  
Hpv16 Infection ◽  
Common Infection

Abstract Background. We studied the cases of single and multiple HPV infection and analyzed the correlation with negative cases, and preneoplastic and neoplastic lesions of the uterine cervix with the aim of making a contribution to the prognostic factor under discussionMethods: 909 women undergoing second level screening because they had been positive at cervical cytology were enrolled. All the patients underwent colposcopy and cervical biopsy with viral genotyping. We divided mHPV infection based on the number of genotypes present: infections with 2 strains, 3 strains, 4 strains and 5 or more strains.Statistical analysis The analysis of the data was made using the χ2 test. Contingency tables were created to evaluate the correlation between single, multiple and CIN2+ infections. Values with p <0.05 were considered statistically significant.Results: The presence of genotype HPV16 in our study was associated with a 12 times greater risk of developing a high-grade lesion, OR = 12.70. The patients with single infections had the highest incidence of CIN2+ (34.1 %) with respect to those with multiple infections (10.6%).When we studied in the mHPV infection the prevalence of the combinations between the genotypes, we found that in mHPV16 infections, the combinations HPV16, 18 and HPV16, 31 were the most frequent (55.5%) in CIN3 lesion.ConclusionsOur results suggest that single HPV infections have a greater risk of developing SCC with respect to multiple infections. Multiple HPV infections are relevant only in the first phase of the lesion (CIN1-CIN2), while they are absent in carcinomas, where infections are of a single genotype. In particular, among multiple infections, HPV16 infection with 2 HR genotypes is associated significantly with CIN2 / CIN3 (21/30) and has 4 times greater risk of developing a high-grade lesion. Thus, it is probable that only specific combinations of HPV (HPV16,18 - HPV 16,31) can be associated with a clinically significant impact, while other combinations can simply be correlated because of a common infection or diagnostic method used. Therefore, multiple HPV16 infections with two high-risk genotypes is a major risk of CIN2/CIN3.

scholarly journals Visual inspection with acetic acid for detection of high grade lesion in atypical squamous cells and low grade squamous intraepithelial lesions from cervical Pap smear

2011 ◽  
Vol 22 (3) ◽  
pp. 145 ◽  
Author(s):  
Methasinee Pothisuwan ◽  
Kamol Pataradool ◽  
Siriwan Tangjitgamol ◽  
Sunamchok Srijaipracharoen ◽  
Sumonmal Manusirivithaya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Visual Inspection ◽  
Pap Smear ◽  
Low Grade ◽  
High Grade ◽  
Squamous Intraepithelial Lesions ◽  
Squamous Cells ◽  
High Grade Lesion ◽  
Intraepithelial Lesions

scholarly journals Comparison of diagnostic value of visual inspection with Lugol’s iodine to conventional Paps smear in detection of colposcopic biopsy proved CIN: a tertiary care experience

2017 ◽  
Vol 6 (12) ◽  
pp. 5477
Author(s):  
Manisha Bajaj ◽  
Shamshad Ahmad ◽  
Shubha S. Trivedi
Keyword(s):  
Cervical Cancer ◽  
Sensitivity And Specificity ◽  
Visual Inspection ◽  
Pap Smear ◽  
Tertiary Care ◽  
Control Programme ◽  
Reproductive Age ◽  
Screening Methods ◽  
Tertiary Care Centre ◽  
Lugol’S Iodine

Background: An organized cytological screening (Pap smear) is the mainstay for cervical cancer prevention and control programme. Financial and logistic burden of operating such a program is considerable. This has prompted the evaluation of alternative feasible techniques. It appeared from the earlier studies that visual inspection with lugol’s iodine (VILI) could be a potential alternative to cervical cytology. VILI is a low cost, easy to apply and highly sensitive technique. It gives result immediately thus allows doctors to “see and treat” at first visit. Objective of present study was to evaluate the performance of visual inspection with lugol’s iodine and its comparison with cytology in cervical cancer screening.  Methods: 400 non-pregnant reproductive age women were subjected to Paps and VILI in this prospective study done in a tertiary-care centre in Delhi. Reference standard used for all was colposcopy and colposcopic-directed biopsy (when required).Results: Of 400 Pap smears done 11.75% were normal, 77.5% inflammatory, 5.5% had ASCUS, 0.25% ASC-H, 0.5% AGUS, 2% LSIL, 1.5% HSIL and invasive cancer in 0.5%. With LSIL and above smears as significant, the sensitivity and specificity of Paps were 50% and 97.66% respectively. VILI was positive in 12.8% of cases. Sensitivity and specificity of VILI was 85.5% and 89.9% respectively. Overall accuracy of VILI in detecting pre-invasive lesion was 89.7% while with pap smear it was 96%.Conclusions: Accuracy of VILI and Pap smear in detecting pre-invasive cervical lesion was comparable. At tertiary-care centers VILI can be used effectively to screen the patients with significant lesions. Patients can be directly subjected to further treatment at the earliest thus reducing loss to follow-up.

Low Utility in Colposcopy-directed Biopsies for Non-high Grade Cytological Abnormalities on PAP Smear

2020 ◽  
Vol 16 (1) ◽  
pp. 18-22
Author(s):  
Eronmwon E. Gbinigie ◽  
Joshua Fogel ◽  
Maggie Tetrokalashvili
Keyword(s):  
Pap Smear ◽  
Multinomial Logistic Regression ◽  
Cervical Dysplasia ◽  
Outcome Variable ◽  
Low Grade ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Intraepithelial Lesion ◽  
Cytologic Abnormalities ◽  
Cytology Screening

Background: Clinicians commonly perform colposcopy directed biopsies on patients with low grade squamous intraepithelial lesion (LSIL) on PAP cytology even when not consistent with clinical guidelines. Objective: We study the association of PAP cytology screening results with cervical intra-epithelia neoplasia (CIN) 2-3 high-grade dysplasia, as confirmed by colposcopy-directed biopsy. Methods: A retrospective study of 263 women with an abnormality on the PAP smear. Multinomial logistic regression was performed with predictors of PAP cytology screening results with the outcome variable of colposcopy-directed biopsy. Results: High grade squamous intraepithelial lesion (HSIL) had significantly increased relative risk for CIN 2-3 (RR: 9.85, 95% CI: 1.84, 52.79, p=0.008). LSIL was not significantly associated with CIN 2-3. In the comparisons of negative with CIN-1, both HSIL and LSIL were not significantly associated with a negative biopsy. Conclusion: HSIL is associated with cervical dysplasia of CIN 2-3 while LSIL is not associated with cervical dysplasia of CIN 2-3. We do not recommend routine biopsies in patients with LSIL cytologic abnormalities unless additional compelling factors exist.

Sign in / Sign up

Export Citation Format

Share Document