ADA activity in the follicular fluid of infertile women with diminished ovarian reserve can act as a predictor of ovarian reserve

Background: Adenosine deaminase (ADA) catalyses the deamination of adenosine to inosine. In the human reproductive tract, the importance of enzymes that affect metabolism of adenosine, particularly ADA, has been emphasized. It is aimed to evaluate the plasma and follicular fluid (FF) activities of total ADA (ADAT) in infertile women and to determine its relation with ovarian reserve markers and in vitro fertilization (IVF) outcomes.Methods: Plasma and FF activities of ADAT were measured in 106 infertile women. Its relation with ovarian reserve markers and IVF outcomes were determined.Results: There was a significant difference in the ADAT activities between plasma and FF of infertile women (p<0.01). The activity of plasma ADAT was higher than FF ADAT in infertile women (p<0.01). The activity of FF ADAT in DOR group was higher than that of the others (p<0.01). In DOR group; the activity of FF ADAT activity had a negative correlation with BMI and a positive correlation with FSH and no relation with IVF outcomes.Conclusions: Increased ADAT activity can lead to reduced adenosine levels, which might be resulted in disturbed fertility process. The activity of FF ADAT activity might be important for fertility work-up. Further studies are needed.

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The effectiveness of collaborative infertility counseling (CIC) on pregnancy outcome in infertile women undergoing in vitro fertilization: A randomized controlled trial

10.21203/rs.2.14093/v1 ◽

2019 ◽

Author(s):

Mahboobeh Rasoulzadeh Bidgoli ◽

robab latifnejad roudsari ◽

ali montazeri

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Rate ◽

Treatment Success ◽

Intervention Group ◽

Control Group ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

And Control

Abstract Background: Infertility is an emotional tension which influences the whole aspects of relationships in infertile couples. A main objective of infertility treatments is elevation of pregnancy rate. The present study aimed to examine the effect of collaborative counseling on pregnancy rate in infertile women, undergoing in vitro fertilization in Mashhad, Iran. Methods: In this clinical trial, 60 women with primary infertility were selected from an infertility research center and were randomly allocated into intervention (n=29) and control (n=31) groups. The intervention group received individual counseling, based on the collaborative reproductive healthcare model with collaboration of a midwife, a gynecologist and a clinical psychologist in five sessions during a two-month period. The control group received routine care. Positive pregnancy test was considered as a criterion of treatment success at the end of the study. Data were analyzed using statistical tests including independent samples t-test. Results: There was no significant difference in pregnancy rate between intervention and control groups (P = 0.298). Also, there were no significant differences in follicle and embryo numbers between two groups. However, a significant difference was observed between two groups in terms of oocyte numbers where the intervention group had more oocyte (P = 0.014). Conclusion: Overall the findings indicated that the collaborative infertility counseling did not improve treatment success in infertile women undergoing in vitro fertilization

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Follicular fluid and urinary concentrations of phthalate metabolites among infertile women and associations with in vitro fertilization parameters

Reproductive Toxicology ◽

10.1016/j.reprotox.2016.04.005 ◽

2016 ◽

Vol 61 ◽

pp. 142-150 ◽

Cited By ~ 48

Author(s):

Yao-Yao Du ◽

Yue-Li Fang ◽

Yi-Xin Wang ◽

Qiang Zeng ◽

Na Guo ◽

...

Keyword(s):

In Vitro Fertilization ◽

Follicular Fluid ◽

Phthalate Metabolites ◽

Vitro Fertilization ◽

Infertile Women

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Faculty Opinions recommendation of Follicular fluid and urinary concentrations of phthalate metabolites among infertile women and associations with in vitro fertilization parameters.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726283150.793522598 ◽

2016 ◽

Author(s):

Lauren Foster

Keyword(s):

In Vitro Fertilization ◽

Follicular Fluid ◽

Phthalate Metabolites ◽

Vitro Fertilization ◽

Infertile Women

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P–653 The levels and ratios of adipokines in the follicular fluid as promising prognostic factors for in vitro fertilization outcomes

Human Reproduction ◽

10.1093/humrep/deab130.652 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

J Ryzhov ◽

A Shpakov ◽

N Tkachenko ◽

M Mahmadalieva ◽

I Kogan ◽

...

Keyword(s):

Prognostic Factors ◽

In Vitro Fertilization ◽

Follicular Fluid ◽

Ovarian Reserve ◽

Uterine Cavity ◽

Normal Body Mass Index ◽

Vitro Fertilization ◽

Ivf Outcomes ◽

Number Of Patients

Abstract Study question Can the adipokines levels and ratios in the follicular fluid (FF) be used to predict in vitro fertilization (IVF) outcomes? Summary answer The leptin level and ratios leptin/ghrelin and leptin/adiponectin in FF are reliable prognostic factors for IVF outcomes in women with normal body mass index (BMI). What is known already The adipose tissue serves not a simple fat storage, rather an important endocrine organ, producing adipokines, such as leptin, adiponectin, ghrelin and others. Adipokines have been shown to regulate the cardiovascular system, food intake, metabolism, inflammation, metastatic spread of tumors,and also reproduction, affecting the activity of the hypothalamo-pituitary-gonadal axis. The plasma and FF adipokines have been used as prognostic factors for IVF outcomes, but the obtained results are controversial. The most promising in this case may be the distribution of patients into groups in accordance with their BMI and a separate study of adipokine ratios in them. Study design, size, duration Women (n = 53), who undergo IVF, were divided on two groups, according to their BMI: normal BMI (18.5–24.9 kg/m2, n = 25) and increased BMI (>25.0 kg/m2, n = 28). Depending on IVF outcomes after the antagonist protocol, the groups formed were subsequently subdivided into two groups each: non-pregnant with normal BMI (nPN, n = 16), pregnant with normal BMI (PN, n = 9), non-pregnant with increased BMI (nPI, n = 21), pregnant with increased BMI (PI, n = 7). Participants/materials, setting, methods: Inclusion criteria for participants were: age 18–45 years and infertility due to male/tubal factor. Exclusion criteria were: polycystic ovarian syndrome (PCOS), diabetes mellitus, or plasma level of anti-Müllerian hormone <1.0 ng/mL. The FF from the first punctated follicle was collected and tested for leptin, adiponectin and ghrelin levels using ELISA kits. If gestational sac was seen in uterine cavity using ultrasound on day 21–25 after embryo transfer, pregnancy was diagnosed. Main results and the role of chance Women with increased BMI had, as a rule, higher FF levels of leptin and the leptin/ghrelin and leptin/adiponectin ratios, compared with women with normal BMI. Furthermore, leptin level was higher in PN as compared to nPN (21.61±2.55 vs 10.54±1.16, p < 0.05), but did not differ in the PI and nPI groups. The same pattern was obtained for the leptin/ghrelin and leptin/adiponectin ratios. The ghrelin level showed an inverse pattern. It was higher in nPN as compared to PN (3.80±0.78 vs 1.39±0.19, p < 0.05), but did not differ in women with increased BMI. The obtained data demonstrate that the association between the adipokine levels in FF and the IVF outcomes is better in women with normal BMI than in women with increased BMI. Adipokines, which differed among the study groups, were evaluated as prognostic factors in women with normal BMI.The values were calculated at which pregnancy became unlikely: leptin level <15 ng/mL, leptin/ghrelin ratio <9, and leptin/adiponectin ratio <5. For each indicators, the sensitivity and specificity were >88%. The predictive power of these indicators in the clinic using the odds ratio (95% confidence interval) was: leptin level 7.11 (1.23–40.99, p < 0.05), leptin/ghrelin 29.53 (1.53–570.83, p < 0.05), and leptin/adiponectin 7.11 (1.23–40.99, p < 0.05). Limitations, reasons for caution The main limitation of the study is a relative small number of patients, although the differences between the investigated groups were significant. Furthermore, women with low ovarian reserve, age > 40 years, endometriosis or PCOS were not included in the study. Wider implications of the findings: The obtained results indicate the good prospects for using such indicators as the adipokines levels and their ratio in FF to predict IVF outcomes in women with low ovarian reserve, endometriosis and PCOS, but with normal BMI. Trial registration number Not applicable

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Effect of intrauterine granulocyte-colony stimulating factor administration on in vitro fertilization outcome in women with moderate-to-severe endometriosis: An RCT

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i8.9621 ◽

2021 ◽

pp. 733-740

Author(s):

Ladan Kashani ◽

Ashraf Moini ◽

Tayebeh Esfidani ◽

Nazila Yamini ◽

Shima Mohiti

Keyword(s):

In Vitro Fertilization ◽

Clinical Pregnancy ◽

Control Group ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

Stimulating Factor

Background: Nearly 25-50% of infertile women have endometriosis. There are reports of disorders in the expression of granulocyte colony-stimulating factor (G-CSF) receptors in women with endometriosis. Objective: To examine the effect of intrauterine administration of G-CSF in in vitro fertilization (IVF) cycles on the fertility rate of infertile women with moderate-to-severe endometriosis. Materials and Methods: This clinical trial was conducted on 66 infertile women with moderate-to-severe endometriosis, undergoing IVF and intracytoplasmic sperm injection (ICSI). The participants were allocated into two groups via simple randomization: the G-CSF (n = 27) and control (n = 39) groups. In the G-CSF intervention group, on the oocyte pick-up day, immediately after an ovarian puncture, 300 μg of G-CSF was injected through a transcervical catheter under abdominal ultrasound guide to visualize flushing into the uterine cavity. Women in the control group received no intervention. The two groups were evaluated for clinical pregnancy. Results: No significant difference was noted in the demographic characteristics of the two groups. The rate of clinical pregnancy was 28.2% in the control group and 25.9% in the G-CSF group, indicating no significant difference (p = 0.83). Conclusion: The results showed that the intrauterine injection of G-CSF had no effects on pregnancy in women with stage-3/4 endometriosis undergoing IVF. Key words: G-CSF, In vitro fertilization, Endometriosis, Pregnancy.

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Association of Iron Status in Follicular Fluid with Pregnancy Outcomes in Infertile Women Undergoing IVF/ICSI

Iraqi Journal of Science ◽

10.24996/ijs.2021.62.6.3 ◽

2021 ◽

pp. 1779-1786

Author(s):

Banan Salam Kadhum ◽

Shatha Abdul Wadood AL- Shammaree

Keyword(s):

Follicular Fluid ◽

Polycystic Ovary ◽

Iron Status ◽

Control Group ◽

Iron Level ◽

Infertile Women ◽

Significant Difference ◽

Ferroxidase Activity ◽

Pcos Group

Iron status may influence the outcome of infertile women under the intracytoplasmic sperm injection (ICSI) technique of in vitro fertilization (IVF). The aim of this study is to evaluate iron status and ceruloplasmin ferroxidase activity in the follicular fluid (FF) and their association with IVF outcomes. The study enrolled fertile women with male cause infertility (n=25), infertile women with polycystic ovary syndrome (PCOS; n=21), infertile women with low AMH level (n=26), and women with unexplained infertility (UI; n=27), all undergoing IVF/ICSI. On the day of oocyte suction, the selection of FF samples was accomplished. Iron, ferritin, and transferrin levels, as well as ceruloplasmin (CP) ferroxidase activity, were measured in the FF. In the PCOS group, iron showed significantly higher level (P<0.05) as compared to the control and UI groups. In the PCOS group, ferritin showed significantly higher level (P<0.05) compared with the control group. In the PCOS group, transferrin showed significantly higher level (P<0.05) when compared with the UI group. Also, Cp. ferroxidase activity in the PCOS group showed a lower level, but non-significant difference, compared with the other groups. In conclusion, the increased iron level in the follicular fluid of women with PCOS may lead to decrease pregnancy success after applying IVF protocol.

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P–196 Bacterial influence on oocyte quality - the secret of a successful fertilization

Human Reproduction ◽

10.1093/humrep/deab130.195 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

M Schenk ◽

E Voroshilina ◽

M Boldyreva ◽

M Koranda ◽

N Reinschissler ◽

...

Keyword(s):

Follicular Fluid ◽

Genital Tract ◽

Reproductive Tract ◽

Female Reproductive Tract ◽

Lactobacillus Species ◽

Bacterial Composition ◽

Vitro Fertilization ◽

Dna Technology ◽

Follicular Fluids

Abstract Study question Is there a difference in bacterial composition of follicular fluid between oocytes developing a good quality blastocyst and oocytes that fail fertilization? Summary answer Follicular fluids of oocytes failing fertilization show a different bacterial profile compared to follicular fluids of oocytes that were successfully fertilized. What is known already The presence of pathogens in the female reproductive tract has been intensively investigated. Lactobacillus species are mainly associated with a healthy genital tract and good prognosis for a successful pregnancy. Studies of the bacterial composition of follicular fluids have been mainly undertaken in women participating in reproductive medicine treatment because of the nature to obtain the specimen. In most studies follicular fluids have been pooled for analysis. Information on separately collected follicular fluids is still rare. We hypothesized that the composition of bacteria within follicular fluids is responsible for the success of the fertilization process. Study design, size, duration The study was designed and conducted at the Kinderwunsch Institut Schenk GmbH (Dobl, Austria) together with DNA-Technology. Follicular fluids from 46 patients undergoing IVF (in vitro fertilization) and ICSI (intracytoplasmic sperm injection) treatment were included and analyzed. Participants/materials, setting, methods Follicular fluids from 46 patients were collected separately. 2 follicular fluids from each patient were screened for common bacteria of the genital tract. One from an oocyte developing a good quality blastocyst and one displaying fertilization failures. Samples were analyzed for bacterial composition using the Femoflor16 (DNA-Technology). Main results and the role of chance Quantitative analysis revealed a higher total bacteria mass in follicles from oocytes that failed fertilization. Furthermore, Lactobacillus were not present in those follicles compared to good blastocyst follicles. In addition, Chlamydia trachomatis was found mainly in follicular fluid of not fertilized oocytes together with Eubacterium, Gardnarella and Trichomonas species. Interestingly, a trend of elevated levels of Ureaplasma species in follicular fluids of oocytes developing good quality blastocysts was observed. Limitations, reasons for caution Contamination of follicular fluids due to the procedure of oocyte pick up and follicular fluid retrieval cannot be completely excluded. Results should be confirmed with a higher sample size. Wider implications of the findings: We assume that different bacterial compositions in follicular fluids are responsible for the destiny of the oocyte. It is tempting to speculate that bacterial analysis of follicular fluids may be beneficial to select to best oocytes in future IVF/ICSI treatments. Trial registration number Not applicable

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The clinical outcome of Dienogest treatment followed by in vitro fertilization and embryo transfer in infertile women with endometriosis

Journal of Ovarian Research ◽

10.1186/s13048-019-0597-y ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 3

Author(s):

Hiroshi Tamura ◽

Hiroaki Yoshida ◽

Hiroyuki Kikuchi ◽

Mai Josaki ◽

Yumiko Mihara ◽

...

Keyword(s):

Oxidative Stress ◽

In Vitro Fertilization ◽

Clinical Outcome ◽

Control Group ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

Ivf Et ◽

Follicular Fluids

Abstract Background Endometriosis is considered to be the most intractable cause of female infertility. Administering any type of treatment for endometriosis before in vitro fertilization and embryo transfer (IVF-ET) is an important strategy for improving the IVF-ET outcomes for infertile women with endometriosis. In fact, treatment with a gonadotropin-releasing hormone (GnRH) agonist just before IVF-ET has been reported to improve the clinical outcome in endometriosis patients. However, the benefit of Dienogest (DNG), a synthetic progestin, treatment just before IVF-ET remains unclear. Methods Sixty-eight infertile women with Stage III or IV endometriosis (ovarian endometrial cyst < 4 cm) were recruited for this study. The subjects were divided into 2 groups: a DNG group (n = 33) and a control group (n = 35). DNG was administered orally every day for 12 weeks prior to the conventional IVF-ET cycle in the DNG group. Standard controlled ovarian hyperstimulation with the GnRH agonist long protocol was performed in the control group. The numbers of mature follicles and retrieved oocytes, fertilization rates, implantation rates, and clinical pregnancy rate were compared between the two groups. In addition, the concentrations of inflammatory cytokines, oxidative stress markers, and antioxidants in follicular fluids were also measured. Results The numbers of growing follicles, retrieved oocytes, fertilized oocytes, and blastocysts were significantly lower in the DNG group than in the control group. The fertilization and blastocyst rates were also lower in the DNG group than in the control group. Although there was no significant difference in the implantation rate between the groups, the cumulative pregnancy rate and live birth rate were lower in the DNG group than in the control group. There was no significant difference in the abortion rate. Our results failed to show that DNG reduces the inflammatory cytokine levels and oxidative stress in follicular fluids. Conclusions Administering DNG treatment just before IVF-ET did not provide any benefits to improve the clinical outcomes for infertile women with endometriosis.

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Steroid Hormones of Follicular Fluid and the Outcome of in Vitro Fertilization

Serbian Journal of Experimental and Clinical Research ◽

10.2478/sjecr-2021-0018 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Aleksandra Gavrilovic ◽

Jelena Cekovic ◽

Aida Parandilovic ◽

Aleksandar Nikolov ◽

Predrag Sazdanovic ◽

...

Keyword(s):

In Vitro Fertilization ◽

Steroid Hormones ◽

Follicular Fluid ◽

Fertilization Rate ◽

Vitro Fertilization ◽

Gynecology And Obstetrics ◽

Clinical Center ◽

Significant Difference ◽

Assisted Fertilization

Abstract One of the succes factors of biomedically assisted fertilization is the regular maturation of one or more oocytes. The quality of the oocytes is significantly influenced by the environment in which it is located, the so-called „microenvironment” that includes cumulus cells, follicular fluid in which hormones and growth factors involved in its growth and development are secreted. The main aim was to examine whether the concentration of steroid hormones in the follicular fluid affects the rate of fertilization and the outcome of the in vitro fertilization process itself. The study included 31 patients who were included in vitro fertilization procedure at the Department for Biomedically Assisted Fertilization, Clinic for Gynecology and Obstetrics, Clinical Center Kragujevac. We used follicular fluid as biological material for analysis. Examination of the obtained follicular fluid and collection of oocytes under a stereomicroscope was done in the embryological laboratory at the Department. Biochemical parameters of follicular fluid were analyzed in the Department for Laboratory Diagnostics, Clinical Center Kragujevac. In vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) methods were used as the fertilization method. The criteria of the Istanbul Consensus of Clinical Embryologists were used as a reference framework for embryo quality assessment. Pregnancy was confirmed by a positive serum level of the hormone β-hCG 14 days after embryo transfer. A software package ЅРЅЅ 20 was used for statistical data processing. The results of the analysis of follicular fluid samples show that there was no statistically significant difference in the concentration of estradiol, progesterone and testosterone in follicular fluid in relation to fertilization rate and the outcome of in vitro fertilization. Based on our results, it can be concluded that the concentration of steroid hormones did not affect fertilization rate and the outcome of in vitro fertilization.

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Vitamin D does not have any Impact on Ovarian Reserve Markers in Infertile Women

Gynecology Obstetrics and Reproductive Medicine ◽

10.21613/gorm.2016.676 ◽

2017 ◽

pp. 1

Author(s):

Ümit Görkem ◽

Ferit Kerim Küçükler ◽

Cihan Toğrul ◽

Şebnem Gülen

Keyword(s):

Body Mass Index ◽

Vitamin D ◽

Waist Circumference ◽

Body Mass ◽

Ovarian Reserve ◽

Hip Circumference ◽

Infertile Women ◽

Significant Difference ◽

Ovarian Reserve Markers ◽

Ovarian Syndrome

Objective: To evaluate the potential role of vitamin D on ovarian reserve markers in infertile women with different reserve patterns.Study design: This prospective cross-sectional study included the infertile women attending the Hitit University Hospital. The initial examination included the measurements of waist circumference and hip circumference, body mass index, and waist/hip ratio. A total of 171 women were divided into three groups according to ovarian reserve patterns: (i) adequate ovarian reserve pattern (AOR, n=77), (ii) high ovarian reserve pattern (polycystic ovarian syndrome, n=62), and (iii) diminished ovarian reserve pattern (DOR, n=32). The serum estradiol (E2), follicle stimulating hormone, total testosterone, 17-hydroxy-progesterone (17(OH) P), dehydroepiandrosterone sulfate, anti-mullerian hormone, and hydroxycholecalciferol (25(OH) D) levels were analyzed. Results: No significant difference between three different groups was detected in terms of body mass index, waist circumference, hip circumference, waist/hip ratio, E2 and 17OHP levels (p>0.05, for all). One-hundred-sixty-nine (98.2%) women in all groups had vitamin D concentration below 30 ng/mL as a cut-off value. The circulating 25 (OH) D levels did not show a significant difference between all ovarian reserve groups (p=0.804). No correlation between 25(OH) D and anti-mullerian hormone levels was documented in women with AOR, polycystic ovarian syndrome and DOR patterns (r=-0.099 p=0.393, r=0.034 p=0.794 and r=0.157 p=0.390 respectively). 25 (OH) D levels were significantly correlated with body mass index, waist circumference and hip circumference parameters only in AOR group (r=-0.355 p=0.002, r=-0.305 p=0.007 and r=-0.322 p=0.004 respectively).Conclusions: There is no association between 25 (OH) D and ovarian reserve markers. 25 (OH) D levels were significantly correlated with body mass index, waist circumference and hip circumference parameters only in AOR group.

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