Effect of autologous platelet-rich plasma in the promotion of healing of chronic ulcers

Background: Chronic ulcers are a big health problem worldwide. Having a great impact at personal, social and professional levels. The use of autologous Platelet Rich Plasma (PRP) is a major advance in the treatment of these ulcers as an easy and cost-effective method. Platelets provide numerous growth factors enhancing tissue healing. The aim of this study was to evaluate the safety and efficacy of autologous platelet-rich plasma as a treatment of chronic non-healing ulcers.Methods: Autologous PRP was prepared from whole blood by centrifugation and activated by 10% calcium chloride. Twenty-Four (24) patients with non-healing ulcers of different etiologies, whom they met our inclusion criteria, were treated with PRP injected every two weeks locally into their wounds until healing. The ulcer dimensions were measured every week. The follow-up period was 12 weeks after healing.Results: The mean age of the study population 41±21 years. Complete healing was achieved in all patients. The mean rate of healing (average decrease in ulcer dimensions) was 0.48 cm/week. The rate of healing was greater at the week following injection. The mean time for healing was 6.11 weeks.Conclusions: Author witnessed the useful effects of PRP application on enhancing wound healing. The results from our case series showed that PRP is a safe and effective treatment for the promotion of healing chronic ulcers. Further research and controlled, randomized prospective clinical trials on larger patient population are important to validate our results.

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Successful Management of Treatment-Resistant Alopecia Areata with Platelet Rich Plasma: A Case Series

Serbian Journal of Dermatology and Venerology ◽

10.2478/sjdv-2019-0007 ◽

2019 ◽

Vol 11 (2) ◽

pp. 50-52

Author(s):

Sanjeewani Fonseka ◽

Y.M.D.M Bandara ◽

Brabaharan Subhani

Keyword(s):

Alopecia Areata ◽

Platelet Rich Plasma ◽

Case Reports ◽

Immunosuppressive Agents ◽

Case Series ◽

Treatment Resistant ◽

Successful Management ◽

Autologous Platelet Rich Plasma ◽

Dermal Injection ◽

Autologous Platelet

Abstract Introduction. Alopecia areata (AA) is an autoimmune disease-causing non-scarring alopecia. It is usually treated with immunosuppressive agents, to which some patients fail to respond adequately. Material and Methods. Three patients with AA refractory to standard therapy were treated with intra-dermal injection of autologous platelet rich plasma (PRP) every four weeks. Results. All three patients showed remarkable improvement after multiple sessions of PRP treatment. Conclusion. Autologous PRP is safe and effective in treatment-resistant forms of AA demonstrated in many case reports; therefore it deserves further study with randomized, placebo-controlled trials.

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Bisphosphonate-related osteonecrosis of the jaws—an initial case series report of treatment combining marginal bone resection and autologous platelet-rich plasma

International Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.ijom.2011.07.152 ◽

2011 ◽

Vol 40 (10) ◽

pp. 1071

Author(s):

M. Curi ◽

C.L. Cardoso ◽

D.H. Koga ◽

C. Zardetto

Keyword(s):

Platelet Rich Plasma ◽

Case Series ◽

Bone Resection ◽

Osteonecrosis Of The Jaws ◽

Case Series Report ◽

Autologous Platelet Rich Plasma ◽

Autologous Platelet ◽

Series Report

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Intradiscal Injection of Autologous Platelet-Rich Plasma Releasate to Treat Discogenic Low Back Pain: A Preliminary Clinical Trial

Asian Spine Journal ◽

10.4184/asj.2017.11.3.380 ◽

2017 ◽

Vol 11 (3) ◽

pp. 380-389 ◽

Cited By ~ 38

Author(s):

Koji Akeda ◽

Kohshi Ohishi ◽

Koichi Masuda ◽

Won C. Bae ◽

Norihiko Takegami ◽

...

Keyword(s):

Low Back Pain ◽

Back Pain ◽

Platelet Rich Plasma ◽

Low Back ◽

Discogenic Low Back Pain ◽

Intradiscal Injection ◽

Autologous Platelet Rich Plasma ◽

The Mean ◽

Autologous Platelet

<sec><title>Study Design</title>Preliminary clinical trial.</sec><sec><title>Purpose</title>To determine the safety and initial efficacy of intradiscal injection of autologous platelet-rich plasma (PRP) releasate in patients with discogenic low back pain.</sec><sec><title>Overview of Literature</title>PRP, which is comprised of autologous growth factors and cytokines, has been widely used in the clinical setting for tissue regeneration and repair. PRP has been shown <italic>in vitro</italic> and <italic>in vivo</italic> to potentially stimulate intervertebral disc matrix metabolism.</sec><sec><title>Methods</title>Inclusion criteria for this study included chronic low back pain without leg pain for more than 3 months; one or more lumbar discs (L3/L4 to L5/S1) with evidence of degeneration, as indicated via magnetic resonance imaging (MRI); and at least one symptomatic disc, confirmed using standardized provocative discography. PRP releasate, isolated from clotted PRP, was injected into the center of the nucleus pulposus. Outcome measures included the use of a visual analog scale (VAS) and the Roland-Morris Disability Questionnaire (RDQ), as well as X-ray and MRI (T2-quantification).</sec><sec><title>Results</title>Data were analyzed from 14 patients (8 men and 6 women; mean age, 33.8 years). The average follow-up period was 10 months. Following treatment, no patient experienced adverse events or significant narrowing of disc height. The mean pain scores before treatment (VAS, 7.5±1.3; RDQ, 12.6±4.1) were significantly decreased at one month, and this was generally sustained throughout the observation period (6 months after treatment: VAS, 3.2±2.4, RDQ; 3.6±4.5 and 12 months: VAS, 2.9±2.8; RDQ, 2.8±3.9; <italic>p</italic><0.01, respectively). The mean T2 values did not significantly change after treatment.</sec><sec><title>Conclusions</title>We demonstrated that intradiscal injection of autologous PRP releasate in patients with low back pain was safe, with no adverse events observed during follow-up. Future randomized controlled clinical studies should be performed to systematically evaluate the effects of this therapy.</sec>

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Intrauterine Infusion of Autologous Platelet-Rich Plasma Affects the Endometrial Thickness, Epidermal Growth Factor and Pregnancy Outcome in Patients Undergoing IUI

Iraqi Journal of Embryos and Infertility Researches ◽

10.28969/ijeir.v9.i2.r2 ◽

2020 ◽

Vol 9 (2) ◽

Author(s):

Wafaa A. Abaid ◽

◽

Manal T. Al-Obaidi ◽

Muayad S. Abood ◽

◽

...

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Endometrial Thickness ◽

Platelet Rich Plasma ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Epidermal Growth ◽

Autologous Platelet Rich Plasma ◽

The Mean ◽

Autologous Platelet

Despite developments in assisted reproductive technology, there is immaterial progress in the implantation and pregnancy rates. Intrauterine infusion (IUIF) of autologous platelet-rich plasma (PRP) might renew implantation rates through its paracrine properties by progression cytokines and growth factors which favor implantation. Here we determine whether the IUIF of autologous PRP had a role in pregnancy outcome through its outcome on epidermal growth factor and endometrial thickness. An overall of 43 patients where prospectively randomly dispersed into two groups subjected to a superovulation program using Letrozole® tablet orally 2.5 mg twice daily 12 hours apart from day 2 for 5 days for one cycle. 20 women were considered as control receiving the conventional intrauterine insemination (IUI) management while 23 of them were given PRP by IUIF on the day of human chorionic gonadotrophin injection. The IUI was done for both groups 36-48 hours after confirming ovulation. The blood samples were collected from both groups on the day of IUI for the valuation of epidermal growth factor and an ultrasound was done on the day of human chorionic gonadotrophin injection and day of IUI for assessment of endometrial thickness. The mean endometrial thickness in the PRP group at the day of IUI was significantly thicker than that of the control group and the difference in percentage change of endometrial thickness between PRP group and controls significantly higher in PRP group. The mean epidermal growth factor and the pregnancy rate were significantly superior in the PRP group than that of controls. In conclusion, autologous PRP IUIF was well-tolerated and resulted in a significant expansion in endometrial thickness, epidermal growth factor Level and, subsequent pregnancy rate in an infertile woman undergoing IUI.

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Bisphosphonate-Related Osteonecrosis of the Jaws–An Initial Case Series Report of Treatment Combining Partial Bone Resection and Autologous Platelet-Rich Plasma

Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.joms.2011.02.078 ◽

2011 ◽

Vol 69 (9) ◽

pp. 2465-2472 ◽

Cited By ~ 54

Author(s):

Marcos Martins Curi ◽

Giuliano Saraceni Issa Cossolin ◽

Daniel Henrique Koga ◽

Cristina Zardetto ◽

Silmara Christianini ◽

...

Keyword(s):

Platelet Rich Plasma ◽

Case Series ◽

Bone Resection ◽

Osteonecrosis Of The Jaws ◽

Case Series Report ◽

Autologous Platelet Rich Plasma ◽

Autologous Platelet ◽

Series Report

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Intraoperative collection of autologous platelet-rich plasma from the cardiopulmonary bypass circuit upon initiation of extracorporeal circulation

Journal of Cardiothoracic Surgery ◽

10.1186/s13019-020-01388-5 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Takeshi Honda ◽

Yuji Kanaoka ◽

Hiroshi Furukawa ◽

Taishi Tamura ◽

Noriaki Kuwada ◽

...

Keyword(s):

Platelet Count ◽

Cardiopulmonary Bypass ◽

Platelet Aggregation ◽

Platelet Rich Plasma ◽

Adenosine Diphosphate ◽

Collection System ◽

Blood Samples ◽

Autologous Platelet Rich Plasma ◽

The Mean ◽

Autologous Platelet

Abstract Objectives The aim of this study is to evaluate the possibility of the autologous platelet-rich plasma (PRP) collection from the cardiopulmonary bypass (CPB) circuit and to evaluate its effect on the aggregative function. Methods For seventy-two patients undergoing cardiac surgery with CPB, an autologous PRP was prepared using the Haemonetics Component Collection System® by drawing blood from the CPB circuit immediately after CPB was established. The blood samples were taken at three points for examination, A: beginning of surgery, B: immediately after heparin reversal with protamine following discontinuation of CPB, C: after the collected autologous PRP was returned to the patient. Platelet count and platelet aggregation ability were analyzed. Results The mean platelet count in autologous PRP was 5.5 (range: 3–14) units. Platelet count decreased by 115.0 (±27.3) × 1000/μl from A to B and increased by 27.3 ± 17.2 (× 1000/μl) from B to C. When platelet aggregation was measured by Adenosine Diphosphate (ADP) 3.0 μM, it decreased by 42.6% ± 12.1% from A to B and increased by 8.7% ± 7.4% from B to C. Conclusions Autologous PRP can be safely collected by drawing blood from the CPB circuit, platelet count and aggregation ability significantly decreased after CPB including autologous PRP collection. Some improvement was detected in the number of the platelets count and platelet aggregation ability by administrating an autologous PRP even if autologous PRP is collected from CPB circuit. Trial registration UMI-CTR, UMIN000023776. Registered 1 October 2016.

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Ultrasound-guided injection of platelet-rich plasma in a patient with an ischeotibial muscle injury (Inyección guiada por ultrasonido de plasma rico en plaquetas en un paciente con lesión en el origen de la musculatura isquiotibial)

Retos ◽

10.47197/retos.v0i41.82520 ◽

2021 ◽

pp. 209-213

Author(s):

Lina Andrea Gomez ◽

Javier Fernando Bonilla Briceño ◽

Angélica Patricia Martinez Vasquez ◽

Ana Luisa Muñoz

Keyword(s):

Muscular Strength ◽

Platelet Rich Plasma ◽

Cost Effective ◽

Sports Activity ◽

Calidad De Vida ◽

Application Techniques ◽

Autologous Platelet Rich Plasma ◽

The Right ◽

Autologous Platelet

Platelet-rich plasma (PRP) is a platelet concentrate that is obtained after centrifugation and may influence tissues healing by growth factors released after platelet degranulation. This treatment has been used for the management of muscles, ligaments, and tendinopathy injuries with promising results, there is still limited clinical evidence for its use. In this study we present the report of a case of a 50-year-old soccer player who presented an injury at the origin of the ischeotibial muscles of the right thigh. On day 13, after the injury, autologous Platelet Rich Plasma (APRP) was applied under ultrasound (US) guidance. The control by US and Nuclear Magnetic Resonance (NMR) was performed two weeks after the PPR application, which show resolution of the hematoma, and tissue regeneration. The patient returned to his sports activity at week 12 after the PRP treatment, without pain and with improvement in muscular strength The PRP treatment could be an option to avoid surgical procedures in athletes because it could improve muscular strength and healing, added it is easy to obtain and apply. Further studies are necessary to reach a consensus regarding clinical indications, processing and PRP application techniques that rebound in alternatives to improve the quality of life of patients in a cost-effective way, as well as shortening the recovery time of athletes. Resumen. El plasma rico en plaquetas (PRP) es un concentrado de plaquetas que se obtiene después de centrifugación y puede influir en la curación de los tejidos por los factores de crecimiento liberados después de la desgranulación de las plaquetas. La terapia con PRP ha sido utilizada para el manejo de lesiones de tejidos blandos, como músculos, ligamentos y tendones con resultados prometedores. aunque la evidencia clínica actual para su uso es limitada. En este estudio presentamos el reporte del caso de un jugador de fútbol de 50 años que presentó lesión en el origen de la musculatura isquiotibial del muslo derecho. El día 13, después de la lesión, se le aplicó Plasma Rico en Plaquetas Autólogo (PRPA) guiado por ultrasonido (US). El control por US y resonancia magnética nuclear (RMN) se realizó dos semanas después de la aplicación del PRP, mostrando recuperación de la lesión y resolución del hematoma. El paciente volvió a realizar actividades deportivas en la semana 12 después del tratamiento, sin dolor y con mejoría de la fuerza muscular. El tratamiento con PRP podría ser una opción para evitar procedimientos quirúrgicos mejorando la fuerza muscular y la curación de la musculatura isqueotibial, adicionalmente el PRP es fácil de obtener y aplicar. De igual manera se necesitan más estudios para llegar a un consenso sobre las indicaciones clínicas, el procesamiento y las técnicas de aplicación de PRP que redunden en alternativas para mejorar la calidad de vida de los pacientes de una manera costo-efectiva, así como acortar el tiempo de recuperación de los atletas.

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