scholarly journals Primary laryngeal tuberculosis

2019 ◽  
Vol 5 (3) ◽  
pp. 777
Author(s):  
Aditya Brahmono ◽  
Nyilo Purnami ◽  
Muhtarum Yusuf
Keyword(s):  
Fiber Optic ◽  
Intensive Therapy ◽  
Systemic Symptom ◽  
Chain Reaction ◽  
Common Causes ◽  
Polymerase Chain ◽  
Microlaryngeal Surgery ◽  
Histopathology Examination ◽  
Continue Therapy ◽  
Laryngeal Tuberculosis

Primary laryngeal tuberculosis (TB) is a very rare disease that became the most common causes of granuloma disease in the larynx. The manifestasion of tuberculosis on the laryngeal is commonly local without systemic symptom. This case reported was 21 year old male that complained of hoarseness for 6 months. The results of the fiber optic laryngoscopy (FOL) is generally believed a papilloma of the larynx. Biopsy extraction with microlaryngeal surgery was a mandatory procedure that apparently showing an overview of TB based on the results of histopathology and polymerase chain reaction (PCR). Anti-tuberculosis drugs were given to the patients, the evaluation carried out six months after the patient obtain intensive therapy and continue therapy with good result. Due to the non spesific laryngeal sign that was observed on clinical examination, clinicians must consider the possibility of primary laryngeal tuberculosis. Biopsy the lession on larynx continued with histopathology examination must not hesitate to confirm the diagnosis. PCR can be considered the better way to detect the TB bacteria. <p> </p>

Case Report: Progressive Dysphonia and Dysphagia due to Laryngeal Leishmaniasis

2021 ◽  
Author(s):  
Laura Renard ◽  
Adrien Lemaignen ◽  
Guillaume Desoubeaux ◽  
David Bakhos
Keyword(s):  
Polymerase Chain Reaction ◽  
Weight Loss ◽  
Case Report ◽  
Amphotericin B ◽  
Laryngeal Cancer ◽  
Complete Resolution ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Histopathology Examination

Laryngeal leishmaniasis is an unusual form of the disease. We report the case of a patient who consulted for dysphonia and dysphagia in a context of asthenia and weight loss. The patient had lesions that were suggestive of laryngeal cancer but were revealed to be leishmaniasis by histopathology examination and polymerase chain reaction. Treatment with amphotericin B and miltefosine permitted complete resolution of the lesions and no recurrence during the 18-month follow-up period.

scholarly journals Hoarseness of Voice as a Rare Presentation of Tuberculosis: A Case Report Study

2019 ◽  
Vol 7 (19) ◽  
pp. 3262-3264
Author(s):  
Taher Felemban ◽  
Abdullah Ashi ◽  
Abdullah Sindi ◽  
Mohannad Rajab ◽  
Zuhair Al Jehani
Keyword(s):  
Laryngeal Carcinoma ◽  
Incidental Finding ◽  
Chain Reaction ◽  
Rare Presentation ◽  
Case Presentation ◽  
X Ray ◽  
History Of ◽  
Chest X Ray ◽  
Polymerase Chain ◽  
Laryngeal Tuberculosis

BACKGROUND: Having hoarseness of voice as the first clinical manifestation of tuberculosis is rare. This atypical presentation causes some confusion since other more common conditions, such as laryngeal carcinoma, present similarly and might require more invasive tests to confirm the diagnosis. CASE PRESENTATION: A 38-year-old male presented to the otorhinolaryngology clinic with a four-month history of change in voice. Laryngoscopy demonstrated a right glottic mass, raising suspicion of laryngeal cancer. The computed tomography showed a mass and incidental finding of opacities in lung apices. Chest x-ray demonstrated findings suggestive of tuberculosis. Polymerase chain reaction and culture of sputum samples confirmed the diagnosis and the patient was started on anti-tuberculosis treatment. CONCLUSION: Despite accounting for only 1% of pulmonary tuberculosis cases and having a similar presentation to laryngeal carcinoma, we recommend considering laryngeal tuberculosis when evaluating hoarseness of voice in endemic areas.

Fiber-Optic Fluorometric Sensing of Polymerase Chain Reaction-Amplified DNA Using an Immobilized DNA Capture Protein

1996 ◽  
Vol 235 (1) ◽  
pp. 61-72 ◽  
Author(s):  
J.Matthew Mauro ◽  
Lynn K. Cao ◽  
Lynne M. Kondracki ◽  
Stephen E. Walz ◽  
James R. Campbell
Keyword(s):  
Polymerase Chain Reaction ◽  
Fiber Optic ◽  
Chain Reaction ◽  
Dna Capture ◽  
Polymerase Chain

scholarly journals First Detection and Characterization of Chrysanthemum virus B infecting Chrysanthemum in Thailand

2021 ◽  
Author(s):  
Salit Supakitthanakorn ◽  
Garnjana Wichitrakoonthavorn ◽  
Kaewalin Kunasakdakul ◽  
On-Uma Ruangwong
Keyword(s):  
Cultural Values ◽  
Ornamental Plants ◽  
Systemic Symptom ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Transmission Electron ◽  
Staining Methods ◽  
Polymerase Chain ◽  
Systemic Symptoms

Abstract Chrysanthemum is one of the important ornamental plants in worldwide due to its high economic and cultural values. Chrysanthemum leaves showed mosaic, ringspot, yellowing and mild mottle symptoms were observed and collected from cultivation areas in northern Thailand and used for detection of important viruses infecting chrysanthemum. Chrysanthemum virus B (CVB) was detected by reverse transcription polymerase chain reaction (RT-PCR) from samples showing yellowing and mild mottle symptoms. Sequences of the coat protein (CP) gene of two CVB isolates found in this study were sequenced and shared 93.15% homology with other CVB isolates from different countries deposited in GenBank. Biological indexing of these CVB found that they induced both local and systemic symptoms in tobacco plants while petunia displayed a systemic symptom. The particles of CVB were observed under transmission electron microscope (TEM), prepared by dip preparation and negative staining methods, showing slightly flexuous rod-shaped virions approximately 600–650 nm in length. To our knowledge, this is the first detection and study on molecular and biological characteristics of CVB infecting chrysanthemum in Thailand.

scholarly journals Genetic testing of hereditary spastic paraplegia

2015 ◽  
Vol 156 (3) ◽  
pp. 113-117 ◽  
Author(s):  
Kinga Hadzsiev ◽  
László Balikó ◽  
Katalin Komlósi ◽  
Anett Lőcsei-Fekete ◽  
Györgyi Csábi ◽  
...  
Keyword(s):  
Hereditary Spastic Paraplegia ◽  
Molecular Testing ◽  
Spastic Paraplegia ◽  
Chain Reaction ◽  
Hereditary Spastic Paraplegias ◽  
Gene Testing ◽  
New Information ◽  
Common Causes ◽  
Polymerase Chain ◽  
Dependent Probe

Introduction: Hereditary spastic paraplegia is the overall term for clinically and genetically diverse disorders characterized with progressive and variable severe lower extremity spasticity. The most common causes of autosomal dominantly inherited hereditary spastic paraplegias are different mutations of the spastin gene with variable incidence in different ethnic groups, ranging between 15–40%. Mutations in the spastin gene lead to loss of spastins function, causing progressive neuronal failure, which results in axon degeneration finally. Aim: The molecular testing of spastin gene is available in the institution of the authors since January, 2014. The experience gained with the examination of the first eleven patients is described in this article. Method: After polymerase chain reaction, Sanger sequencing was performed to examine the 17 exons of the spastin gene. Multiplex ligation-dependent probe amplification was performed to detect greater rearrangements in the spastin gene. Eight of the patients were examined in the genetic counseling clinic of the authors and after detailed phenotype assessment spastin gene testing was obtained. The other three patients were referred to the laboratory from different outpatient clinics. Results: Out of the 11 examined patients, four different pathogenic mutations were found in 5 patients. Conclusions: The first Hungarian data, gained with the examination of spastin gene are presented in this article. The five patients, in whom mutations were detected, represent 45.5% of all tested patients with hereditary spastic paraplegia, which is similar to those published in the international literature. Molecular testing and subsequent detailed genotype-phenotype correlations of the Hungarian patients may serve valuable new information about the disease, which later on may influence our therapeutic possibilities and decisions. Orv. Hetil., 2015, 156(3), 113–117.

DNA amplification and multiplexing

2018 ◽  
Author(s):  
Pierre Taberlet ◽  
Aurélie Bonin ◽  
Lucie Zinger ◽  
Eric Coissac
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Dna Amplification ◽  
Chain Reaction ◽  
Substantial Loss ◽  
Common Causes ◽  
Different Types ◽  
Polymerase Chain ◽  
Tagging System

After a brief reminder of the principles underlying the polymerase chain reaction (PCR), Chapter 6 “DNA amplification and multiplexing” discusses the choice of a DNA polymerase for the PCR. In particular, it warns against the use of proofreading polymerases, that can lead to a substantial loss of PCR specificity. Chapter 6 insists on the benefits of including different types of controls in the PCR (e.g., PCR negatives and positives, tagging system controls, etc.). The most common causes of PCR failures and their solutions are addressed, as well as the precautions to take to avoid and monitor contaminations. Chapter 6 also deals with the particular case of blocking oligonucleotides, which aim at reducing the amplification of undesired sequences. It gives some valuable guidelines to design such oligonucleotides and use them efficiently. Finally, Chapter 6 presents different strategies for tagging individual samples during the amplification, to allow subsequent multiplexing during the sequencing step.

scholarly journals Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuli Melisa Sierra-Arguello ◽  
Gustavo Perdoncini ◽  
Laura Beatriz Rodrigues ◽  
Luciana Ruschel dos Santos ◽  
Karen Apellanis Borges ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Latin American ◽  
Foodborne Diseases ◽  
Chain Reaction ◽  
Virulence Markers ◽  
Pathogenic Genes ◽  
Common Causes ◽  
Polymerase Chain ◽  
Latin American Countries ◽  
Insight Into

AbstractCampylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology.

scholarly journals Detection of Cutaneouse Leishmaniasis species via PCR in Salah Adeen and Baghdad provences

2019 ◽  
Vol 24 (1) ◽  
pp. 57
Author(s):  
Maysaa Ibrahim Al-Jubori1 ◽  
Abd Alrahman A. Al-Tae2 ◽  
, Mohammad A. Al-Faham3
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Age Groups ◽  
Parasitic Diseases ◽  
Age Group ◽  
Chain Reaction ◽  
Common Causes ◽  
Polymerase Chain ◽  
Leishmania Parasites ◽  
Group 1

Background: Leishmaniasis is a parasitic diseases that are spread worldwide due to various species of Leishmania, which are infect mammales diversity as well as human. L. tropica,    L. major, and L. aethiopica which is common causes of cutaneous Leishmaniasis in Salah Adeen and Baghdad provences. Material and Methods: The present study was conducted to investigate the prevalence of cutaneous Leishmaniasis and to identify Leishmania parasites by using polymerase chain reaction (PCR) in some endemic areas of Iraq. A total of 117 samples of patients with suspected cutaneous Leishmaniasis were collected in different age groups. And both sexes 73 male and 44 female patients. Results: PCR results showed the percentage of infections 62.39% of males while 37.60% of females. The average age was 23.35 years (the range, from 1- 60 years), with the highest percentage of cases in the age group 1-4 years and the lowest rate in the age group (40-60 years). The highest infection was by L.tropica of L.major and lowest infection caused by L.aethiopica, where is considered first revealed in Iraq. Conclusion: The study found that males were more likely to be infected than females. The study revealed that polymerase chain reaction (PCR) is the most effective and sensitive method for detecting types of Cutaneous Leishmaniasis.    http://dx.doi.org/10.25130/tjps.24.2019.009

scholarly journals Urokinase Gene 3′-UTR T/C Polymorphism Is Associated with Malignancy and ESRD in Idiopathic Membranous Nephropathy

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Cheng-Hsu Chen ◽  
Shih-Yin Chen ◽  
Kuo-Hsiung Shu ◽  
Mei-Chin Wen ◽  
Chi-Hung Cheng ◽  
...  
Keyword(s):  
Membranous Nephropathy ◽  
Healthy Subjects ◽  
Clinical Manifestations ◽  
Idiopathic Membranous Nephropathy ◽  
Genotype Distribution ◽  
Chain Reaction ◽  
Common Causes ◽  
Disease Entities ◽  
Polymerase Chain ◽  
The Relationship

Idiopathic membranous nephropathy (MN) is one of the most common causes of nephrotic syndrome in adults, and 25% of MN patients proceed to ESRD. Urokinase plasminogen activator (uPA) may play an important role in reducing renal fibrosis. This study was conducted to clarify the relationship between uPA gene polymorphisms and clinical manifestations of MN. We recruited 91 biopsy-diagnosed MN patients and 105 healthy subjects. Genotyping of uPA gene 3′-UTR T/C polymorphism was performed by polymerase chain reaction methods. The genotype distribution had no effect on the development of MN. Thirteen patients (15.9%;P=0.008) acquired malignancies and seventeen (20.7%;P=0.006) patients progressed to ESRD with the C/C genotype, but no patients with the T/C genotype did. In conclusion, we demonstrated that the presence of the uPA gene 3′-UTR C/C genotype was associated with ESRD as well as acquired malignancies in MN patients. These findings should prompt specific considerations for the treatment of MN patients to maintain a balance between treating disease entities and protecting the immune system from cancers.

scholarly journals Detection of Parvalbumin, a Common Fish Allergen Gene in Food, by Real-Time Polymerase Chain Reaction

2009 ◽  
Vol 92 (1) ◽  
pp. 234-240 ◽  
Author(s):  
Min Sun ◽  
Chengzhu Liang ◽  
Hongwei Gao ◽  
Chao Lin ◽  
Mingjun Deng
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Food Industry ◽  
Food Hypersensitivity ◽  
Regulatory Agencies ◽  
Chain Reaction ◽  
Common Causes ◽  
Ige Mediated ◽  
Polymerase Chain ◽  
Potential Tool

Abstract Fish, as one of the most common causes of IgE-mediated food hypersensitivity, has recently received increasing attention from the food industry and legislative and regulatory agencies. A real-time polymerase chain reaction assay based on TaqMan-MGB probe technology was developed for the detection of parvalbumin, a major fish allergen gene. The assay had a sensitivity up to 5 pg purified fish DNA and had no cross-reaction with other species, such as cattle, sheep, swine, chicken, shrimp, lobster, crab, squid, clam, rice, soybean, maize, and potato. The coefficient of variation for both intra- and interexperimental variability demonstrated high reproducibility and accuracy. The assay proved to be a potential tool for the detection and label management of fish allergens in food.

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