Role of p53 as a prognostic marker in breast carcinoma and its correlation with tumor size, tumor grade and lymph node metastasis

11061 Background: We recently reported that the HERmark assay (Monogram Biosciences) accurately measures continua of total HER2 expression (H2T) and HER2 homodimers (H2D) over a wide (∼3 logs) dynamic range, and that a higher concordance was observed between H2T and HER2 status with more stringent central tests as compared with IHC tests performed locally (Joensuu et al, 2008 SABCS,abstract 2071). H2D/H2T ratio was reported as a marker of activated HER2 and a prognosticator of disease progression in HER2+ patients not treated with trastuzumab in the adjuvant setting (Bates et al, 2008 SABCS,abstract 1074). In this follow-up analysis, H2T, H2D, and H2D/H2T ratio were correlated with histopathologic characteristics of breast cancers in the FinHer study. Methods: The HERmark assay was used to measure H2T and H2D in 899 formalin-fixed, paraffin-embedded FinHer specimens. The results were correlated with histopathologic characteristics of breast cancers in the FinHer study (Joensuu et al, N Engl J Med2006;354), including estrogen receptor/progesterone receptor (ER/PR), tumor grade, tumor size, lymph node metastasis, and stage. Results: Higher H2T and H2D levels correlated with ER/PR negativity and high tumor grade (P<0.0001). 42% (102/244) of ER- and 37% (137/374) of PR- cases were HERmark Positive; while 17% (110/655) of ER+ and 14% (75/524) of PR+ cases were HERmark Positive. 10% (13/136) of grade 1, 18% (65/353) of grade 2, and 35% (131/375) of grade 3 tumors were HERmark Positive. No significant association was found between H2T or H2D and tumor size, lymph node metastasis or stage. ER/PR negative and poorly differentiated cancers had higher H2D/H2T ratios (P=0.013), and H2D/H2T ratios >0.6 were associated with smaller primary tumor diameters at the time of cancer detection (P=0.009). Conclusions: The quantitative H2T measurement confirms the known correlations between HER2 expression and histopathologic characteristics of breast cancer. The novel H2D measurement and H2D/H2T ratio may provide further insights into HER2 activation and better diagnostic tests for targeted HER2 therapy. [Table: see text]

Download Full-text

STUDY ON RELATION BETWEEN TRADITIONAL PROGNOSIS FACTORS AND HORMONE RECEPTORS STATUS, KI-67, HER2 IN INVASIVE BREAST CARCINOMA

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2017.1.11 ◽

2017 ◽

pp. 70-76

Author(s):

Cong Thuan Dang ◽

Thi Thu Thuy Phan ◽

Nam Dong Tran ◽

Cao Sach Ngo ◽

Thi Hong Van Vo

Keyword(s):

Lymph Node ◽

Breast Carcinoma ◽

Lymph Node Metastasis ◽

Tumor Size ◽

Hormone Receptors ◽

Histological Grade ◽

Invasive Breast Carcinoma ◽

Disease Stage ◽

Ki 67 ◽

Node Metastasis

Objectives: To examine histopathologic features and hormone receptors status, Ki-67 and HER2 in invasive breast carcinoma at Hue University Hospital to evaluating the relation between tumor size, histological grade, lymph node metastasis status, disease stage and the expression of steroid hormone receptors status, Ki-67 and HER2 in invasive breast carcinoma. Materials and methods: From May 2015 to April 2016, samples were collected from 96 breast carcinoma patients. Histopathologic samples were stained by Hematoxylin-Eosin and immunohistochemistry staining at Pathology Department, Hue University Hospital. R esults: Most invasive breast carcinoma patients were among the age of 50-59 (39.6%), invasive ductal carcinoma (82.3%), tumor size ≤2cm (54.2%), histological grade II (60.2%), lymph node metastasis (53.1%), disease stage II (51%); ER(+) 46.9%, PR(+) 49.0%, Ki-67(+) 77.1%, HER2(+) 30.2% of cases. Conclusion: There were a positive correlation between histological grade with the HER2 expression (p<0.05) in invasive breast carcinoma. Key words: invasive breast carcinoma, immunohistochemistry, tumor size, histological grade, lymph node metastasis, disease stage

Download Full-text

Tumor grade 2 as the independent predictor for lymph node metastasis in 10–20 mm sized rectal neuroendocrine tumor

Korean Journal of Clinical Oncology ◽

10.14216/kjco.21006 ◽

2021 ◽

Vol 17 (1) ◽

pp. 37-43

Author(s):

Byung-Soo Park ◽

Sung Hwan Cho ◽

Gyung Mo Son ◽

Hyun Sung Kim ◽

Su Jin Kim ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Tumor Size ◽

Tumor Grade ◽

Radical Resection ◽

Independent Factor ◽

Ki 67 ◽

Node Metastasis ◽

Significant Difference ◽

National University

Purpose: Rectal neuroendocrine tumors (NETs) <10 mm are endoscopically resected, while those ≥20 mm are treated with radical surgical resection. The choice of treatment for 10–20 mm sized rectal NETs remains controversial. This study aimed to verify factors predicting lymph node metastasis (LNM) of 10–20 mm sized rectal NET and utilize them to decide upon the treatment strategy.Methods: Twenty-eight patients with 10–20 mm sized rectal NETs treated at Pusan National University Yangsan Hospital from January 2009 to September 2020 were divided into LNM (+) and LNM (–) groups, and their respective data were analyzed.Results: Seven patients (25%) had LNM while 21 patients (75%) did not. Endorectal ultrasound findings showed tumor size was significantly larger in the LNM (+) than in the LNM (–) group (15 mm vs. 10 mm, P=0.018); however, pathologically, there was no significant difference in tumor size (13 mm vs. 11 mm, P=0.109). The mitotic count (P=0.011), Ki-67 index (P=0.008), and proportion of tumor grade 2 patients (5 cases, 71% vs. 1 case, 5%; P=0.001) were significantly higher in the LNM (+) group. In multivariate analysis, tumor grade 2 was the independent factor predicting LNM (odds ratio, 61.32; 95% confidence interval, 3.17–1,188.64; P=0.010).Conclusion: Tumor grade 2 was the independent factor predicting LNM in 10–20 mm sized rectal NETs. Therefore, it could be considered as the meaningful factor in determining whether radical resection is necessary.

Download Full-text

Sonographic Features of Pure Mucinous Brelast Carcinoma With Micropapillary Pattern

Frontiers in Oncology ◽

10.3389/fonc.2021.644180 ◽

2021 ◽

Vol 11 ◽

Author(s):

Wu Zhou ◽

Yong-Zhong Li ◽

Li-Min Gao ◽

Di-Ming Cai

Keyword(s):

Lymph Node ◽

Breast Carcinoma ◽

Lymph Node Metastasis ◽

Tumor Size ◽

Invasive Growth ◽

Acoustic Feature ◽

Node Metastasis ◽

Missed Diagnosis ◽

Micropapillary Pattern ◽

Mucinous Breast Carcinoma

ObjectivePrevious studies have mostly discussed the clinical manifestations and prognosis of mucinous breast carcinoma with a micropapillary pattern. The purposes of this study were to investigate the sonographic features of pure mucinous breast carcinoma with micropapillary pattern (MUMPC) and to identify the role of ultrasound in the differential diagnosis between MUMPC and conventional pure mucinous breast carcinoma (cPMBC).Materials and MethodsWe obtained written informed consent from all patients, and the Ethics Committee of West China Hospital approved this retrospective study. The study was conducted between May and August 2020. We enrolled 133 patients with 133 breast lesions confirmed as mucinous breast carcinoma (MBC) histopathologically between January 2014 and January 2020.We retrospectively assessed sonographic features (margin, shape, internal echogenicity, calcification, posterior acoustic feature, invasive growth, blood flow grade, and rate of missed diagnosis) and clinical characteristics (age, tumor size, tumor texture, initial symptom, and lymph node metastasis). Bivariable analyses were performed using SPSS version 19.0.ResultsThe 133 lesions included 11 MUMPCs, 65 cPMBCs, and 57 mixed MBCs (MMBCs). There were significant differences in margin, shape, calcification, posterior acoustic feature, invasive growth, rate of missed diagnosis, average tumor size, and lymph node metastasis among the three groups (p < 0.05). The subsequent pairwise comparisons showed that there were significant differences in lymph node metastasis, margin, and invasive growth between MUMPC and cPMBC (p < 0.05). In patients aged >45 years, there was a significant difference in tumor size among the three groups (p = 0.045), and paired comparison showed that the average tumor size in the cPMBC group was larger than that in the MMBC group (p = 0.014).ConclusionMUMPC showed a non-circumscribed margin and invasive growth more frequently than cPMBC did. Lymphatic metastasis was more likely to occur in MUMPC than cPMBC. Ultrasound is helpful to distinguish MUMPC from cPMBC.

Download Full-text

Role of Expression of Cell Cycle Inhibitor p27 and MIB-1 in Predicting Lymph Node Metastasis in Male Breast Carcinoma

The Breast Journal ◽

10.1046/j.1524-4741.2002.08206.x ◽

2002 ◽

Vol 8 (2) ◽

pp. 101-107 ◽

Cited By ~ 4

Author(s):

Joseph Anderson ◽

Vijaya B. Reddy ◽

Linda Green ◽

Pincas Bitterman ◽

Raphael Borok ◽

...

Keyword(s):

Cell Cycle ◽

Lymph Node ◽

Breast Carcinoma ◽

Lymph Node Metastasis ◽

Male Breast ◽

Node Metastasis ◽

Cell Cycle Inhibitor ◽

Male Breast Carcinoma ◽

Mib 1

Download Full-text

Predictors of lymph node metastasis in T1 colorectal cancer in young patients: Results from a national cancer registry.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.e15590 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. e15590-e15590

Author(s):

Jameel Kenneth Singh ◽

Daryl Ramai ◽

Peter Bhandari ◽

Tarik Kani ◽

Mohamed Barakat MD ◽

...

Keyword(s):

Colorectal Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Tumor Size ◽

Young Patients ◽

Tumor Grade ◽

Cancer Specific Survival ◽

Node Metastasis ◽

T1 Colorectal Cancer

e15590 Background: The incidence of colorectal cancer in young adults ( < 44 years) is on the rise. Identifying young patients at risk of regional lymph node metastasis (LNM) is crucial for long-term survival. Prior studies have examined rates of LMN in average risk patients. The objective of this study is to fill the knowledge gap by examining predictors of LMN in young patients using a national cancer registry. Methods: Patients diagnosed with T1 colorectal cancer were identified in the Surveillance, Epidemiology, and End Results register 2000-2017. Potential predictors of LNM and its impact on cancer-specific survival were assessed in logistic and Cox regression with and without multivariable adjustment. Results: In total, 692 patients with T1 colorectal cancer were identified. Most tumors occurred in White race (77.7%), between 40-44 years of age (49.4%), grade III tumor differentiation (59.8%), 1 to 1.9 cm in size (32.2%), and were left sided tumors (61.1%). The overall LNM rate was 22.5 % (n = 149). Using multivariate analysis, LMN was associated with tumor grade IV (undifferentiated) (odds ratio (OR) 2.94, CL: 1.06-8.12; p = 0.038), and increasing tumor size (1 cm – 1.9 cm: OR 2.92, CL: 1.71-4.97, p < 0.001; 2.0 cm – 2.9 cm: OR 2.00, CI: 1.05-3.77, p = 0.034; and ≥ 3.0 cm: OR 2.68, CI: 1.43-5.01, p = 0.002). Five-year cancer-specific survival for patients with LNM was 91% and for patients without LNM 98%. Adjusted cox proportion models showed that LMN was associated with four times higher rate of mortality (hazard ratio (HR) 4.43, CI: 1.27-15.52, p = 0.020). Conclusions: In conclusion, the overall LNM rate is approximately 22% for T1 CRC in young patients (less than 45 years). Tumor size and tumor grade are significant predictors for LNM in patients with T1 CRC cancer. Moreover, positive lymph node involvement is a significant prognostic factor for cancer specific survival. Thus, careful preoperative assessment of lymph node status is essential in clinical decision making, to achieve better long-term outcomes

Download Full-text

Correlation ER/PR status of Breast Carcinoma with Tumor size, Tumor grade, Lymphovascular emboli, Lymph node metastasis and the distant metastasis

IP Archives of Cytology and Histopathology Research ◽

10.18231/j.achr.2021.043 ◽

2021 ◽

Vol 6 (3) ◽

pp. 191-196

Author(s):

Vaibhav Mane ◽

Nekta Anand ◽

Darshana Wakkar

Keyword(s):

Breast Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Tumor Size ◽

Tumor Grade ◽

Tumor Type ◽

Receptor Status ◽

Node Metastasis ◽

Poor Predictor ◽

Size Grade

Breast cancer is the most frequent cancer in females in the world and is the second most common cancer in India after cervical cancer. The breast cancer is second most common reason of loss of existence in each developed and additionally most of the growing countries. For the appraisal of prognosis of breast cancer commonly followed is the NOTTINGHAM PROGNOSTIC INDEX (NPI) which includes- tumor size, histological tumor grade, lymph node metastasis and hormone receptor status. These prognostic component help in administration and therapeutic requirement of breast cancer patient.1. To study the MRM specimens for size, grade of the tumor, LVI and LN metastases and the ER/PR receptors of the tumor. 2.To study the association of ER/PR status with the above mentioned prognostic parameters.This three-year study includes 50 histopathologically confirmed cases of carcinoma breast. The tumor type, grade, LNM, LVE were reported on H & E. The ER-PR study was done of all 50 cases. The tumor size, grade, NM, LVE were correlated with receptor status. Out of 50 cases majority (7.5%) of cases were in 41-50 years of age group, and tumor size was between 2-4 cm in (17.5%) cases; majority were of grade II carcinoma (17.5%) of which 13% were ER-PR positive. Out of 35 cases with negative LNM and LVE, 26 cases (13%) were ER-PR positive.In our observation there is no association between higher histological grade and ER-PR status. No obvious correlation with tumor size was noted. But increase in tumor size could also be a poor predictor of ER-PR status. LNM, LVE is poor predictor of ER-PR status.

Download Full-text

EVALUATION OF CLINICAL AND PARACLINICAL FEATURES IN PATIENTS WITH CARCINOMA OF ORAL CAVITY

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.5.9 ◽

2016 ◽

pp. 56-60

Author(s):

Van Minh Nguyen ◽

Hong Loi Nguyen ◽

Thi Kim Anh Dang

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Oral Cavity ◽

Tumor Size ◽

Regional Lymph Node ◽

Regional Lymph Node Metastasis ◽

Female Ratio ◽

Histopathological Grade ◽

Node Metastasis ◽

Metastasis Rate

Background: To evaluate the clinical, hystopathologycal features and correlation between lymph node metastasis and hystopathologycal grade in patients with carcinoma of the oral cavity. Materials and Methods: From July 2015 to July 2016, 32 patients with carcinoma of the oral cavity at Hue Central Hospital Results: The most common age group from 51 to 60 years and the male/female ratio was 1.9/1. Tumor were usually observed around the the tongue (40.6%) and oral floor (34.4%). Most of the tumor size is larger than 2 cm diameters (> 80%). The regional lymph node metastasis rate was 43.8% and there was a positive correlation between lymph node metastasis and tumor size (p <0.05). Squamous-cell carcinoma was mainly type of histopathology. Difference between the rate of lymph node metastasis in patient groups with different histopathological grade show no statistical significance (p> 0.05). Conclusion: the greater tumor, the higher regional lymph node metastasis. There is no relationship between the lymph node metastasis rate and histopathological grade of oral carcinoma. Key words: : carcinoma of oral cavity, tumor size, lymph node metastasis, histopathology

Download Full-text