scholarly journals Role of solid phase immunoassay as a screening test for antinuclear antibody – A comparative analysis on Indian population

2021 ◽  
Vol 6 (4) ◽  
pp. 278-282
Author(s):  
Samrat Bordoloi ◽  
Sharath Kumar ◽  
Jayaram Iyenger ◽  
Nagaraj S ◽  
Yatish G C ◽  
...  
Keyword(s):  
Pilot Study ◽  
Specific Antibody ◽  
Predictive Value ◽  
Screening Test ◽  
Antinuclear Antibody ◽  
Solid Phase ◽  
Antibody Detection ◽  
Specific Antibodies ◽  
Solid Phase Immunoassay ◽  
Antibody Tests

Detection of Antinuclear antibody (ANA) is the hallmark of laboratory investigations in Connective Tissue Disorders (CTD). However, various methodologies used in both screening tests and specific antibody detection has led to a loss of consensus and poor reproducibility of results. The objective of this study is to compare Solid Phase Immunoassay (SPI) with Indirect Immunofluorescence (IFA) as a screening test in correlation with the clinical profile as well as subsequent detection of specific antibodies. The study was conducted as a pilot study with a sample size of 60 cases, recruited by Rheumatologists, between April 2019 to July 2019. Each sample was screened by IFA and SPI and tested for specific antibodies by three different specific antibody tests. Although the Sensitivity of SPI (71%) was lower when compared to IFA (79%), the Specificity (78%), Positive Predictive Value (PPV) (74%) and Negative Predictive Value (NPV) (76%) were all comparatively higher. In two clinically proven cases of Sjogren’s syndrome where IFA was negative and SPI was positive, specific antibody tests showed positivity for SSA/Ro. Also it was seen in two clinically confirmed cases of Systemic Lupus Erythematosus IFA was positive and SPI was negative. In this pilot study SPI appeared comparable to IFA as a screening test with better specificity, PPV and NPV. The utility of SPI was especially seen in cases with antibodies against SSA/Ro where IFA may be negative. However, in a few cases of high antibody titer SPI appeared to give a false negative result.

scholarly journals Antibody responses to rhesus cytomegalovirus glycoprotein B in naturally infected rhesus macaques

2003 ◽  
Vol 84 (12) ◽  
pp. 3371-3379 ◽  
Author(s):  
Yujuan Yue ◽  
Shan Shan Zhou ◽  
Peter A. Barry
Keyword(s):  
Specific Antibody ◽  
Transmembrane Domain ◽  
Solid Phase ◽  
Rhesus Macaques ◽  
Antibody Responses ◽  
Full Length ◽  
Glycoprotein B ◽  
Primate Model ◽  
Specific Antibodies ◽  
Rhesus Cytomegalovirus

Rhesus cytomegalovirus (RhCMV) exhibits strong parallels with human CMV (HCMV) in terms of nucleic and amino acid identities, natural history, and mechanisms of persistence and pathogenesis in its natural host, rhesus macaques (Macaca mulatta). To determine whether this non-human primate model would be useful to assess vaccine strategies for HCMV, host immune responses to RhCMV glycoprotein B (gB) were evaluated in RhCMV-infected monkeys. Total protein extracts were prepared from cells transiently transfected with an expression plasmid for either the full-length gB or a derivative (gBΔ, 1–680 aa) lacking both the transmembrane domain and cytoplasmic tail. Western blot analysis showed identical reactivity of macaque sera with full-length gB and its derivative gBΔ, indicating that the immunodominant epitopes of gB are contained in the extracellular portion of the protein. Using gBΔ extract as a solid phase, a sensitive and specific ELISA was established to characterize gB antibody responses in monkeys acutely and chronically infected with RhCMV. During primary infection (seroconversion), gB-specific antibodies developed concurrently and in parallel with total RhCMV-specific antibodies. However, during chronic infection gB-specific antibody responses were variable. A strong correlation was observed between neutralizing and gB-specific antibody levels in RhCMV-seropositive monkeys. Taken together, the results of this study indicate that, similar to host humoral responses to HCMV gB, anti-gB antibodies are an integral part of humoral immunity to RhCMV infection and probably play an important protective role in limiting the extent of RhCMV infection. Thus, the rhesus macaque model of HCMV infection is relevant for testing gB-based immune therapies.

509: The predictive value of donor specific antibody responses in pediatric heart recipients: A pilot study

2007 ◽  
Vol 26 (2) ◽  
pp. S243
Author(s):  
R.J. Boucek ◽  
D. Yung ◽  
K. Nelson ◽  
P. Hopkins ◽  
L. Permut ◽  
...  
Keyword(s):  
Pilot Study ◽  
Specific Antibody ◽  
Predictive Value ◽  
Antibody Responses

scholarly journals Current State of Human Melioidosis Serodiagnostics

2011 ◽  
pp. 18-22 ◽  
Author(s):  
N. P. Khrapova ◽  
V. V. Alekseev
Keyword(s):  
Specific Antibody ◽  
Solid Phase ◽  
Antibody Detection ◽  
Laboratory Practice ◽  
Correct Treatment ◽  
Current State ◽  
Indirect Hemagglutination ◽  
Analytical Review ◽  
Indirect Hemagglutination Test ◽  
Selection Of

This analytical review is devoted to matters of human melioidosis serodiagnostics and prospects of its development and enhancement. Materials of the publications cited reflect particular significance of the specific antibody detection, for both the early and retrospective diagnostics of human melioidosis, as well as for the correct treatment of patients. Summarized are the data on modern approaches to the selection of serodiagnostics methods in the endemic and non-endemic areas, on the advantages and limitations of the most widely applicable methods for the specific antibody detection (indirect hemagglutination test and solid-phase ELISA). In recent years, development of commercially available enzyme-linked test systems for the detection of antibodies to human melioidosis agent has become an object of intense interest, as this will provide for solid-phase ELISA implementation into the laboratory practice for early detection of melioidosis cases in humans.

scholarly journals Class-specific antibody responses to Mycoplasma pulmonis in sera and lungs of infected and vaccinated mice

1980 ◽  
Vol 29 (3) ◽  
pp. 1160-1168
Author(s):  
G Taylor ◽  
C J Howard
Keyword(s):  
Specific Antibody ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Antibody Responses ◽  
Mycoplasma Pulmonis ◽  
Serum Antibodies ◽  
Specific Antibodies ◽  
Immunoglobulin Class ◽  
Immunofluorescence Studies ◽  
Intravenous Inoculation

Class-specific antibodies were measured by a solid-phase microradioimmunoassay in the sera and lung washings of mice after intranasal or intravenous inoculation with live Mycoplasma pulmonis and after systemic, intranasal, or combined vaccination with Formalin-inactivated mycoplasmas. After intranasal or intravenous inoculation with live organisms, serum antibodies were first detected in immunoglobulin M (IgM) followed by IgG2, IgG1, and IgA classes, but significant levels of IgA developed only in those mice inoculated intranasally. The appearance of antibodies in lung washings was later than in serum, but again these were predominantly IgG2 and IgG1. After inoculation with killed organisms, serum antibodies were predominantly IgG1, although IgG2, IgM, and, in intranasally vaccinated mice, IgA were also present. Only IgG1 was detected in lung washings from mice vaccinated systemically, but IgA and IgG2 were present in addition in animals vaccinated intranasally. Immunofluorescence studies indicated that some antibody in lung washings from the latter group of animals was produced locally. A comparison of the levels of various class-specific antibodies and resistance to intranasal challenge suggested that local antibody of any immunoglobulin class is capable of mediating resistance in the lungs to M. pulmonis infection.

scholarly journals Comparison on Serum Levels of Procalcitonin of Children with Viral and Bacterial Infection

2013 ◽  
Vol 2 (3) ◽  
pp. 123-126
Author(s):  
Ming-ming Wang ◽  
Su-nan Cui ◽  
Yan-xue Gong
Keyword(s):  
Bacterial Infection ◽  
Viral Infection ◽  
Likelihood Ratio ◽  
Predictive Value ◽  
Solid Phase ◽  
Positive Likelihood Ratio ◽  
Negative Likelihood Ratio ◽  
Serum Levels ◽  
Solid Phase Immunoassay

Abstract Objective To compare and analyze serum levels of procalcitonin (PCT) of children with viral and bacterial infection and probe into the importance of determining the level of serum PCT in the diagnosis of bacterial infection in order to provide evidences of the clinical use of antibiotics. Methods A total of 85 cases of children with an average age of 8.9 years (10 months -12 years) were enrolled in this study, 53 cases were with viral infection and 32 cases with bacterial infection. We determined serum levels of PCT by semi-quantitative solid phase immunoassay, and the serum levels of PCT were divided into four grades as <0.5 μg / L, ≥ 0.5μg / L, ≥ 2.0μg / L and ≥ 10μg / L for x2 test and Ridit analysis. Results The serum level of PCT of the group with bacterial infection were significantly higher than that of the group with viral infection (P < 0.001). The sensitivity of diagnosis of bacterial infection in children with determination of serum levels of PCT was 87.50% while the specificity was 92.13%, and positive predictive value was 73.68% while negative predictive value was 91.49%, and positive likelihood ratio was 4.65 while negative likelihood ratio was 0.15, and the diagnostic accuracy was 83.53%. Conclusions Serum PCT is a bacterial sensitive marker of bacterial infection in children, and the determination of the level of serum PCT is helpful for the diagnosis of bacterial infection, which can also be a basis for the use of antibiotics.

Predictive value of HLA variants for Graves' disease recurrence - pilot study in Czech patients

2018 ◽  
Author(s):  
Daniela Vejrazkova ◽  
Josef Vcelak ◽  
Eliska Vaclavikova ◽  
Marketa Vankova ◽  
Petra Lukasova ◽  
...  
Keyword(s):  
Pilot Study ◽  
Predictive Value ◽  
Disease Recurrence ◽  
Graves Disease

DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR

2016 ◽  
Vol 21 (3) ◽  
pp. 261
Author(s):  
Ranti Permatasari ◽  
Aryati Aryati ◽  
Budi Arifah
Keyword(s):  
Hepatitis C ◽  
Predictive Value ◽  
Core Protein ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Hcv Infection ◽  
Antibody Detection ◽  
Hcv Rna ◽  
Hcv Antibody

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to the recipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid test that could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multiple antibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. The samples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infection test using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatography method showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictive value of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody pattern was four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies. Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method has a good diagnostic value.

scholarly journals Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Victor M. Petrone-Garcia ◽  
Raquel Lopez-Arellano ◽  
Gabriela Rodríguez Patiño ◽  
Miriam Aide Castillo Rodríguez ◽  
Daniel Hernandez-Patlan ◽  
...  
Keyword(s):  
Pilot Study ◽  
Broiler Chickens ◽  
Solid Phase ◽  
Oxidation Products ◽  
Specific Pathogen Free ◽  
Post Challenge ◽  
Eimeria Maxima ◽  
Lipid Oxidation Products ◽  
Challenge Control ◽  
Specific Pathogen

AbstractThe purpose of this pilot study was to evaluate and determine the concentration of prostaglandin GF2α (PGF2α) and isoprostane 8‐iso‐PGF2α in plasma and intestine of specific pathogen-free (SPF) Leghorn chickens challenged with Eimeria maxima, with or without dietary supplementation of curcumin using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Eighty 1-day-old male SPF chickens were randomly allocated to one of four groups with four replicates (n = 5 chickens/replicate). Groups consisted of: (1) Control (no challenge), (2) Curcumin (no challenge), (3) Eimeria maxima (challenge), and (4) Eimeria maxima (challenge) + curcumin. At day 28 of age, all chickens in the challenge groups were orally gavaged with 40,000 sporulated E. maxima oocysts. No significant differences (P > 0.05) were observed in the groups regardless of the treatment or challenge with E. maxima. Enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days and 9 days post-challenge were significantly increased (P < 0.01) compared to the non-challenge control chickens. Interestingly, the enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days post-challenge were significantly reduced in chickens fed curcumin, compared to control chickens challenge with E. maxima. At 9 days post-challenge, only levels of isoprostane 8‐iso‐PGF2α in the enteric samples were significantly reduced in chickens challenged with E. maxima supplemented with curcumin, compared with E. maxima challenge chickens. No differences of isoprostane 8‐iso‐PGF2α or PGF2α were observed in plasma at both days of evaluation. Similarly, no significant differences were observed between the challenge control or chickens challenge with E. maxima and supplemented with curcumin at both times of evaluation. The results of this pilot study suggests that the antioxidant anti-inflammatory properties of curcumin reduced the oxidative damage and subsequent intestinal mucosal over-production of lipid oxidation products. Further studies to confirm and extend these results in broiler chickens are required.

Performance Evaluation of the Phosphorescent Porphyrin Label:  Solid-Phase Immunoassay of α-Fetoprotein

2002 ◽  
Vol 74 (22) ◽  
pp. 5845-5850 ◽  
Author(s):  
Tomás C. O'Riordan ◽  
Aleksi E. Soini ◽  
Dmitri B. Papkovsky
Keyword(s):  
Performance Evaluation ◽  
Solid Phase ◽  
Solid Phase Immunoassay
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