scholarly journals Changes in the Structure of the Benzylpenicillin Sodium Salt Molecule under the Pulsed Magnetic Field

2021 ◽  
Vol 7 (1) ◽  
pp. 010305
Author(s):  
Natalia Rodenko ◽  
Valentina Zhukova ◽  
Tatyana Vasilieva ◽  
Vladimir Glushchenkov ◽  
Irina Belyaeva
Keyword(s):  
Magnetic Field ◽  
Sodium Salt ◽  
High Intensity ◽  
Fourier Spectrum ◽  
Amide Group ◽  
Pulsed Magnetic Field ◽  
Drug Molecule ◽  
Applied Frequency ◽  
Fourier Spectra ◽  
Benzylpenicillin Sodium

The study aims at the Fourier spectra of an antibiotic exposed to a high-intensity pulsed magnetic field. The applied frequency was f = 40 kHz, with a number of pulses n = 1, at intensities of the pulsed magnetic field H = 0.09 × 106 А/m, Н = 0.50 × 106 А/m, Н = 0.82 × 106 А/m. It was assumed that under the conditions of the pulsed magnetic field there might be a change in the structure of the drug molecule. The research has revealed that an increase in the pulsed magnetic field power leads first to a decrease in the intensity of the band in the Fourier spectrum, and then to its increase. In addition, changes were recorded in the amide group of benzylpenicillin sodium salt molecules depending on the intensity of the pulsed magnetic field applied to the antibiotic.

scholarly journals Investigation of safety applications benzylpenicillin sodium salt after exposure to a pulsed magnetic field

2019 ◽  
Vol 58 (6) ◽  
pp. 123-129
Author(s):  
Natalia A. Rodenko ◽  
◽  
Tatiana I. Vasilieva ◽  
Peter Р. Purygin ◽  
Irina A. Belyaeva ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Free Radicals ◽  
Sodium Salt ◽  
Intraperitoneal Administration ◽  
Antibacterial Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Model Systems ◽  
Pulsed Magnetic Field ◽  
Benzylpenicillin Sodium

Pulsed magnetic fields are widely used in various fields of modern technology. In our previous works it was shown the strengthening of antibacterial properties of benzylpenicillin sodium salt after exposure to a pulsed magnetic field of high tension. The reason for this may be a change in the conformation of β-lactam antibiotics under external physical influence, which increases the affinity of benzylpenicillin molecules to the active center of penicillin-binding proteins. This article presents studies on the safety of benzylpenicillin sodium salt irradiated by a pulsed magnetic field. Benzylpenicillin sodium salt in powdered form was irradiated with a pulsed magnetic field with the intensity H = (0.09∙106-1.23∙106) A/m, frequency f = 30-70 kHz, and a different number of pulses (n = 1-3), using inductors – single-turn and multi-turn. The influence of pulsed magnetic field on chemiluminescence parameters of irradiated benzylpenicillin sodium salt was studied. The appearance of free radicals in irradiated preparations was evaluated in simple model systems simulating the most common free radical oxidation reactions in the body and in environments in which the formation of reactive oxygen species and lipid peroxidation reactions was initiated. Acute toxicity in intraperitoneal administration of antibiotic to mice before and after treatment with pulsed electromagnetic field with calculation and comparison of LD50 indices was also investigated. It was found that the treatment of the dry substance benzylpenicillin sodium salt by a pulsed magnetic field does not cause the formation of free radicals and is not accompanied by an increase in the toxicity of this antibiotic. The possibility of using new ways to increase the antibacterial activity of antibiotics without increasing their concentrations is considered.

scholarly journals Changes in the activity of various platelet antiplatelet agents when exposed to a high-intensity pulsed magnetic field

2019 ◽  
Vol 59 (9) ◽  
pp. 117-124
Author(s):  
Irina A. Belyaeva ◽  
◽  
Vladimir A. Glushchenkov ◽  
Natalia A. Rodenko ◽  
Tatiana I. Vasilyeva ◽  
...  
Keyword(s):  
Magnetic Field ◽  
High Intensity ◽  
Enzyme Inhibitors ◽  
Antiplatelet Agents ◽  
Modern Technology ◽  
Pulsed Magnetic Field ◽  
Antiplatelet Activity ◽  
Camp Phosphodiesterase ◽  
Significant Difference ◽  
The Impact

Pulse magnetic fields are widely used in modern technology. We studied the antiaggregation properties of pentoxifylline and aspirin irradiated by a pulsed magnetic field at certain parameters: intensity H, frequency f, number of pulses n. The impact of a pulsed magnetic field was carried out both on powdered and dissolved drugs in solution. The aim of the study is to study the effect of high-intensity PMF on the antiplatelet activity of pentoxifylline and aspirin Evaluation of the anti-aggregation effect was carried out using the method of thromboelastography. on a TEG 5000 apparatus. In the analysis of thromboelastograms, the general coagulation tendency was, the functional activity of platelets and fibrinogen, the activity of fibrinolysis, and the physicomechanical properties of the formed clots were determined. Since thrombosis poses a threat to human health, today the use of antiplatelet agents for the prevention of cardiovascular disease is beyond doubt. It was found that exposure to a pulsed magnetic field causes significant changes in the antiplatelet activity of only the pentoxifylline molecule. The effect of PMF on the aspirin molecule has not been identified. This is due, in one hand, to a significant difference in the structure of molecules, as well as to the reaction mechanism in which the studied antiplatelet agents are involved. It can be predicted that the effect of pulsed fields on drugs, which are heterocyclic compounds, antimetabolites, and competitive enzyme inhibitors, can enhance the biological effect of these drugs. A hypothesis was put forward to increase the antiplatelet activity of pentoxifylline by exposure to PMF associated with a change in the conformation of the molecule and an increase in affinity for the active site of cAMP-phosphodiesterase.

scholarly journals CHANGE IN THE ACTIVITY OF PENTOXYPLIN UNDER THE ACTION OF A PULSE MAGNETIC FIELD DEPENDING ON THE DRUG COMPOSITION

2020 ◽  
Vol 22 (5) ◽  
pp. 23-27
Author(s):  
N.A. Rodenko ◽  
◽  
I.A. Belyaeva ◽  
A.V. Samorodov ◽  
◽  
...  
Keyword(s):  
Magnetic Field ◽  
Medicinal Product ◽  
High Intensity ◽  
Antiplatelet Agent ◽  
Pulsed Magnetic Field ◽  
Pure Substance ◽  
Single Turn ◽  
Abrupt Changes ◽  
Antiaggregatory Activity ◽  
Intensity Magnetic Field

According to the results of the study, it was found that irradiation of the tabletted preparation of pentoxifylline leads to an increase in its antiaggregatory activity. The aim of this work is to study the effect of a pulsed magnetic field of high intensity on the antiaggregatory activity of the pure substance of petoxifylline. When exposed to a pulsed magnetic field on the pure substance of pentoxifylline (without the presence of excipients), changes in the antiaggregatory activity of the drug were not detected. Irradiation with a pulsed magnetic field was carried out on an MIU-15 setup at various magnetic field strengths with single-turn and multi-turn inductors with the number of pulses n = 1. The authors put forward a hypothesis that excipients in a tabletted medicinal product affect the change in biological activity after exposure to the drug by a pulsed magnetic field of high intensity. The study of the pure substance of pentoxifylline for antiaggregatory activity was carried out on an AT-02 platelet aggregation analyzer. Control samples of the medicinal product were not exposed to the magnetic field. It is assumed that the presence of excipients in the tablet form of pentoxifylline after exposure to a pulsed magnetic field can cause a change in the conformation of the drug molecule. In addition, a transition from one conformation to another is possible due to abrupt changes in properties, i.e. when exposed to a high-intensity magnetic field. An antiplatelet agent with excipients, being in a magnetic field, goes into a more active state, after which it retains it for some time, in which an increase in pharmacological activity is manifested.

scholarly journals Forming a convex flange by pulsed-magnetic field pressure

2021 ◽  
Vol 1047 (1) ◽  
pp. 012011
Author(s):  
N V Kurlaev ◽  
K A Matveev ◽  
M E Ahmed Soliman ◽  
M B Detinov
Keyword(s):  
Magnetic Field ◽  
Pulsed Magnetic Field
Sign in / Sign up

Export Citation Format

Share Document