Análise comparativa do efeito redutor de colesterol do extrato de semente e casca de uva em ratos Wistar

Os flavonóides são compostos fenólicos presentes na maioria das plantas. Têm um importante papel na prevenção e tratamento da aterosclerose, pois, atuam como agentes antiaterogênicos e hipocolesterolêmicos. Objetivou-se nessa pesquisa estudar o efeito redutor de colesterol do extrato de semente e de casca de uva (Vitis vinifera) em ratos Wistar submetidos a uma dieta hipercalórica (ração acrescida de 10% de óleo vegetal) no período de 20 semanas, a partir da 4ª semana de vida. A amostra, composta por 30 espécimes e distribuída entre o Grupo Controle - A (com apenas dieta hipercalórica, n=10) o Grupo Experimental Casca de Uva - B (dieta hipercalórica + extrato de casca de uva, n=10) e Grupo Experimental Semente de Uva – C (dieta hipercalórica + de extrato de semente de uva, n=10). Os resultados mostraram em média, uma redução dos níveis séricos de colesterol total de 14% e de 17,65% em relação ao grupo controle, respectivamente para os animais com dieta a base de casca e de semente de uva. Não houve diferença estatisticamente significativa na redução dos valores de colesterol entre os animais dos dois grupos, não sendo possível assim determinar qual componente da uva representa a melhor alternativa na prevenção e controle da hipercolesterolemia.Comparative analysis of reducing seed extract of cholesterol and effect bark uva in rats WistarAbstract: Flavonoids are phenolic compounds present in most plants. They play an important role in the prevention and treatment of atherosclerosis, as hypocholesterolemic and antiatherogenic act as agents. This research aimed to study and compare the cholesterol-lowering effect of seed extract and grape skin (Vitis vinifera) in rats subjected to a high calorie diet (increased ration of 10% vegetable oil) in the 20-week period, from the 4th week of life. The sample consisted of 30 specimens and distributed between the Control - A (with only high calorie diet, n = 10) Experimental Group Grape Bark - B (high calorie diet + grape skin extract, n = 10) and experimental group Grape Seed - C (calorie diet + grape seed extract, n = 10). The results showed on average a reduction in serum total cholesterol by 14% and 17.65% in the control group, respectively, for the animals with diet based on bark and grape seed. There was no statistically significant difference in reducing cholesterol levels in the animals of both groups, so it is not possible to determine which grape component represents the best alternative in the prevention and control of hypercholesterolemia.

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MORPHOMETRIC, INSTRUMENTAL AND LABORATORY PARAMETERS OF FEMALE RATS WITH EXPERIMENTAL METABOLIC SYNDROME

Здобутки клінічної і експериментальної медицини ◽

10.11603/1811-2471.2020.v.i3.11578 ◽

2020 ◽

pp. 20-25

Author(s):

O. A. Hrygorieva ◽

Y. V. Korotchuk

Keyword(s):

Metabolic Syndrome ◽

Water Regime ◽

Female Rats ◽

Control Group ◽

Laboratory Parameters ◽

The Metabolic Syndrome ◽

Calorie Diet ◽

Experimental Group ◽

High Calorie Diet ◽

High Calorie

The aim of the study – to learn the dynamics of changes of morphometric, instrumental and laboratory parameters in mature females rats with experimental metabolic syndrome. Material and Methods. 20 females of white, mature laboratory rats, aged 18–20 months were divided into 2 groups. The first one is an experimental group: 13 female rats with experimental metabolic syndrome; the second one – control group: 7 intact rats, with standard food and water regime. When working with animals, the standards of the Council of Europe Bioethics Convention 1997, the European Convention for the Protection of Vertebrate Animals were observed. Instruments used during scientific research were subject to metrological control. The simulation of the metabolic syndrome occurred during 60 days. The females supported a special high-calorie diet (grain with margarine 82 % milk fat, corn and sunflower seeds). The water regime included a 20 % solution of fructose and regular water ad libitum, with change every other day. Also, during the first and the fourth weeks of the experiment, the female daily subcutaneously administered Dexamethasone solution at a dosage of 0.1 mg/kg. Results. Since the beginning of the experiment, female rats who received a special high-calorie diet showed a statistically significant increase in all morphometric and instrumental indexes compared to similar rats in the control group. An increase in body weight in the experimental group was found to be 28.93 % higher than the original weight, was observed arterial hypertension (141/85±5) mmHg, dyslipidemia: elevated total cholesterol (5.37±0.33) mmol/L and TG (2.55±0.24) mmol/L; elevated level glucose (8.52±0.17) mmol/L. The above indicators are criteria indicating the presence of metabolic syndrome in animals under study. Conclusions. The proposed model of experimental metabolic syndrome, which includes subcutaneous administration of Dexamethasone solution at a dosage of 0.1 mg/kg in the first and the fourth weeks of experiment, with a special high calorie diet and a 20 % solution of fructose, is an effective way to reproduce the metabolic syndrome in small rodents.

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Grape seed proanthocyanidins and metformin act by different mechanisms to promote insulin signaling in rats fed high calorie diet

Journal of Cell Communication and Signaling ◽

10.1007/s12079-013-0210-x ◽

2013 ◽

Vol 8 (1) ◽

pp. 13-22 ◽

Cited By ~ 17

Author(s):

Baskaran Yogalakshmi ◽

Saravanan Bhuvaneswari ◽

S. Sreeja ◽

Carani Venkatraman Anuradha

Keyword(s):

Insulin Signaling ◽

Grape Seed ◽

Grape Seed Proanthocyanidins ◽

Calorie Diet ◽

High Calorie Diet ◽

High Calorie

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Treat CF-Related Diabetes With Insulin, High-Calorie Diet

Pediatric News ◽

10.1016/s0031-398x(06)71279-6 ◽

2006 ◽

Vol 40 (9) ◽

pp. 9

Author(s):

BRUCE JANCIN

Keyword(s):

Calorie Diet ◽

High Calorie Diet ◽

High Calorie

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The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

Current Issues in Pharmacy and Medical Sciences ◽

10.2478/cipms-2020-0008 ◽

2020 ◽

Vol 33 (1) ◽

pp. 38-44

Author(s):

Alona Yurchenko ◽

Daryna Krenytska ◽

Olexii Savchuk ◽

Tetiana Halenova ◽

Natalia Raksha ◽

...

Keyword(s):

Lipid Peroxidation ◽

Oxidative Modification ◽

Resistance Development ◽

Oxidative Modification Of Proteins ◽

Kidney Beans ◽

Calorie Diet ◽

Diet Control ◽

High Calorie Diet ◽

High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

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Faculty Opinions recommendation of Resveratrol improves health and survival of mice on a high-calorie diet.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1052795.506569 ◽

2006 ◽

Author(s):

Craig Harms

Keyword(s):

Calorie Diet ◽

High Calorie Diet ◽

High Calorie

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Comparative Evaluation of Immediate Bond Strength to Bleached Enamel Following Application of Various Antioxidant Solutions

Dental Journal of Advance Studies ◽

10.1055/s-0038-1672088 ◽

2017 ◽

Vol 05 (02) ◽

pp. 084-089

Author(s):

Alisha Dhingra ◽

Ashu Gupta ◽

Anshu Minocha ◽

Nayantara Sen

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

The Other ◽

Sodium Ascorbate ◽

Group V ◽

Group I ◽

Significant Difference

Abstract Aim: The aim of this study was to compare the reversal of shear bond strength of composite to bleached enamel immediately after bleaching followed by application of various antioxidant solutions. Material and Methods: Seventy central incisors were divided into seven groups. Groups I and II served as unbleached and bleached controls respectively. Groups III, IV, V, VI and VII served as the experimental groups and were subjected to 37.5% hydrogen peroxide bleaching followed by 10 min application of 10% sodium ascorbate, 25% alpha-tocopherol, 6.5% grape seed extract, 5% lycopene and 5% green tea extract respectively. Following composite bonding, shear bond strength was determined and the results were analyzed using ANOVA followed by Post Hoc Multiple Comparisons test. Results: The bond strength values for Group I (positive control) were maximum and significantly different than all the other groups except Grape seed extract group (Group V). When compared to Group II (bleached control), all the groups showed significantly higher bond strength. Significant difference in the bond strength values were seen between Group III (10% sodium ascorbate) and Group V. Also values for Group V were significantly different from Group VI (5% lycopene). All the other values showed insignificantly different results. Conclusion: All the antioxidant solutions improved the shear bond strength values after bleaching but only Grape seed extract application reversed the values to the non bleached levels. Lycopene was least effective. Other antioxidants showed comparable results.

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