scholarly journals MORPHOMETRIC, INSTRUMENTAL AND LABORATORY PARAMETERS OF FEMALE RATS WITH EXPERIMENTAL METABOLIC SYNDROME

2020 ◽  
pp. 20-25
Author(s):  
O. A. Hrygorieva ◽  
Y. V. Korotchuk
Keyword(s):  
Metabolic Syndrome ◽  
Water Regime ◽  
Female Rats ◽  
Control Group ◽  
Laboratory Parameters ◽  
The Metabolic Syndrome ◽  
Calorie Diet ◽  
Experimental Group ◽  
High Calorie Diet ◽  
High Calorie

The aim of the study – to learn the dynamics of changes of morphometric, instrumental and laboratory parameters in mature females rats with experimental metabolic syndrome. Material and Methods. 20 females of white, mature laboratory rats, aged 18–20 months were divided into 2 groups. The first one is an experimental group: 13 female rats with experimental metabolic syndrome; the second one  – control group: 7 intact rats, with standard food and water regime. When working with animals, the standards of the Council of Europe Bioethics Convention 1997, the European Convention for the Protection of Vertebrate Animals were observed. Instruments used during scientific research were subject to metrological control. The simulation of the metabolic syndrome occurred during 60 days. The females supported a special high-calorie diet (grain with margarine 82 % milk fat, corn and sunflower seeds). The water regime included a 20 % solution of fructose and regular water ad libitum, with change every other day. Also, during the first and the fourth weeks of the experiment, the female daily subcutaneously administered Dexamethasone solution at a dosage of 0.1 mg/kg. Results. Since the beginning of the experiment, female rats who received a special high-calorie diet showed a statistically significant increase in all morphometric and instrumental indexes compared to similar rats in the control group. An increase in body weight in the experimental group was found to be 28.93 % higher than the original weight, was observed arterial hypertension (141/85±5) mmHg, dyslipidemia: elevated total cholesterol (5.37±0.33) mmol/L and TG (2.55±0.24) mmol/L; elevated level glucose (8.52±0.17) mmol/L. The above indicators are criteria indicating the presence of metabolic syndrome in animals under study. Conclusions. The proposed model of experimental metabolic syndrome, which includes subcutaneous administration of Dexamethasone solution at a dosage of 0.1 mg/kg in the first and the fourth weeks of experiment, with a special high calorie diet and a 20 % solution of fructose, is an effective way to reproduce the metabolic syndrome in small rodents.

scholarly journals Expediency of application of animals with the spontaneous hypertensia for modelling of the metabolic syndrome

2012 ◽  
Vol 10 (4) ◽  
pp. 91-94
Author(s):  
Maria Alexandrovna Kovaleva ◽  
Marina Nikolayevna Makarova ◽  
A. I. Selezneva ◽  
Valeriy Gennadyevich Makarov
Keyword(s):  
Metabolic Syndrome ◽  
Visceral Obesity ◽  
Cafeteria Diet ◽  
Blood Concentrations ◽  
Spontaneously Hypertensive ◽  
The Metabolic Syndrome ◽  
Calorie Diet ◽  
Wistar Kyoto ◽  
High Calorie Diet ◽  
High Calorie

When used within 11 weeks of diet “cafeteria diet” in spontaneously-hypertensive animals could lead to an increase in systolic blood pressure by 9%, sustained hyperglycemia, increased blood concentrations of triglycerides and cholesterol, as well as an increase in the relative content of visceral fat. In normotensive animals line Wistar-Kyoto prolonged use of “cafeteria diet” was accompanied only by an increase in blood glucose, cholesterol and triglycerides. Thus, the spontaneously hypertensive animals, the application of high-calorie diet demonstrated by three criteria pathogenesis of the metabolic syndrome: hyperglycemia, hypertension and visceral obesity, which allows you to use the model for the study of drugs aimed at treatment of metabolic syndrome.

A high calorie diet causes memory loss, metabolic syndrome and oxidative stress into hippocampus and temporal cortex of rats

Synapse ◽  
2015 ◽  
Vol 69 (9) ◽  
pp. 421-433 ◽  
Author(s):  
Samuel Treviño ◽  
Patrícia Aguilar-Alonso ◽  
Jose Angel Flores Hernandez ◽  
Eduardo Brambila ◽  
Jorge Guevara ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Temporal Cortex ◽  
Memory Loss ◽  
Calorie Diet ◽  
And Oxidative Stress ◽  
High Calorie Diet ◽  
High Calorie

Comparative effects of intragastric Lys-Arg-Аrg-Lys-Pro-Gly-Pro peptides, warfarin, and acetylsalicylic acid on hemostasis indexes and blood glucose in development of metabolic syndrome in rats

2021 ◽  
pp. 52-59
Author(s):  
Л.А. Ляпина ◽  
Н.Ф. Мясоедов ◽  
Т.А. Шубина ◽  
Л.А. Андреева ◽  
Т.Ю. Оберган ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
Acetylsalicylic Acid ◽  
Prothrombin Time ◽  
Carbohydrate Metabolism ◽  
Intragastric Administration ◽  
Calorie Diet ◽  
Time Test ◽  
High Calorie Diet ◽  
High Calorie

Введение. Препараты разной структуры - углеводной, пептидной, белковой оказывают значительный противосвертывающий эффект в кровотоке с одновременным улучшением углеводного обмена. Цель - изучение в сравнительном аспекте влияния препаратов разной структуры (пептида, производного диоксикумарина и ацетилсалициловой кислоты -АСК) на свертывание крови, изменение углеводного обмена при интрагастральном способе их введении крысам. Методика. Использовались стандартные коагулологические методы и способы определения уровня глюкозы крови крыс. Каждый из препаратов (пептид Lys-Arg-Arg-Lys-Pro-Gly-Pro, варфарин и АСК) вводили лабораторным крысам Wistar интрагастрально в эффективной дозе (100 мкг/кг - пептид и варфарин и 1 мг/кг - АСК) в течение 7 сут на фоне развития метаболического синдрома, индуцируемого высококалорийной диетой (ВКД). Определения производили через 20 и 168 ч после последнего введения препаратов при продолжающемся постоянном кормлении крыс ВКД. Результаты. Установлено, что как через 20 ч, так и через 168 ч после последнего введения пептида и АСК агрегация тромбоцитов имела тенденцию к снижению и составляла 72-76% (через 20 ч) и 81-66,7% (через 168 ч); фибринолиз статистически значимо повышался при действии пептида на 61-180%, АСК - на 15-41%, варфарина - на 14-34%; активированное частичное тромбопластиновое время значимо удлинялось под влиянием пептида и варфарина на 24-52 и 31-52% соответственно; свертывание крови по тесту протромбинового времени снижалось только под влиянием варфарина (на 12.3%); уровень глюкозы крови нормализовался под влиянием всех использованых препаратов и составлял 4,9-6,5 ммоль/л против 8.1-8.8 ммоль/л при метаболическом синдроме. Заключение. При сравнении действия пептида, варфарина и АСК установлены гипокоагуляционные и гипогликемические эффекты в разной степени. Максимальным антикоагулянтным и фибринолитическим действием обладал пептид; варфарин проявлял антикоагулянтное действие только по тесту протромбиновое время, ацетилсалициловая кислота обладала антитромбоцитарным и фибриндеполимеризационным действием. Drugs with different structure, carbohydrates, peptides, and proteins, can produce a significant anticoagulation effect and simultaneously improve carbohydrate metabolism. The aim of this study was to compare effects of drugs with different structure, a peptide, a dioxicoumarin derivative, and acetylsalicylic acid (ASA), on coagulation and changes of carbohydrate metabolism in intragastric administration to rats. Methods. Standard methods for studying coagulation and measuring blood glucose in rats were used. Each of the study drugs (Lys-Arg-Arg-Lys-Pro-Gly-Pro peptide, warfarin, and ASA) was administered to Wistar rats intragastrically at an effective dose (100 mcg/kg for the peptide and warfarin and 1 mg/kg for ASA) for 7 days during the development of metabolic syndrome (MS) induced by a high-calorie diet (HCD). Measurements were performed at 20 and 168 h after the last administration of the drugs with continuing HCD. Results. Both at 20 and 168 h after the last administration of the peptide and ASA, platelet aggregation showed a tendency to a decrease and was 72-76% (at 20 h) and 81-66.7% (at 168 h); fibrinolysis significantly increased under the action of the peptide, ASA, and warfarin by 61-180%, 15-41%, and 14-34%, respectively. Activated partial thromboplastin time significantly increased under the action of the peptide and warfarin by 24-52% and 31-52%, respectively; blood clotting as estimated in the prothrombin time test decreased only under the action of warfarin by 12.3%; blood glucose returned to a normal level under the action of each of the three study drugs and was 4.9-6.5 mmol/l vs. 8.1-8.8 mmol/l in MS. Conclusion. The peptide, warfarin, and ASA produced different degrees of the anticoagulation and hypoglycemic effects. The peptide had the strongest anticoagulation and fibrinolytic effects, warfarin produced an anticoagulant effect only according to the prothrombin time test, and acetylsalicylic acid exerted both antiplatelet and fibrin-depolymerizing effects.

scholarly journals The thiol-disulfide homeostasis and its role in the pathogenesis of the experimental alimentary obesity

2016 ◽  
Vol 15 (3) ◽  
pp. 419-423
Author(s):  
Mariya Marushchak ◽  
Inna Krynytska ◽  
Lyudmyla Mazur ◽  
Svitlana Yastremska ◽  
Nina Begosh
Keyword(s):  
Adipose Tissue ◽  
Medical Science ◽  
Redox System ◽  
Control Group ◽  
White Rats ◽  
The World ◽  
Calorie Diet ◽  
Alimentary Obesity ◽  
High Calorie Diet ◽  
High Calorie

Objective: According to WHO, about 30 % of people in the world are overweight that allows to characterize this disease as a new non-infection “epidemic” of the XXI century. More than 500 million people in the world are overweight and 250 million are obese. There is a clear tendency to increasing of alimentary obesity among people with different age, sex and nationality. The aim of the study is to investigate the thiol-disulfide homeostasis in liver tissue, adipose tissue and erythrocytes in the pathogenesis of experimental alimentary obesity.Materials and methods: 60 males, non-liner, white rats around 3 months of age with alimentary obesity were examined during the study. Experimental obesity was modeled by administering of sodium glutamate to the feed mixture in a ratio of 0.6:100.0 and adding high-calorie diet. The glutathione redox-system activity in erythrocytes, liver and adipose tissue were analyzed by the level of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR) and glutathione peroxidase (GP) activity.Results and Discussion: The data indicate a decrease in GSH level within 14 days of the experiment in all investigated tissues. The same trend was observed in animals on 28th day of the experiment: GSH index decreased in blood, adipose tissue and liver (P<0.05). The index of GSSG have increased on 28th day of the experiment in all investigated tissues vs control group (P<0.05). The ratio of the reduced and oxidized forms of glutathione contents was much lower vs control group in all the studied tissues within 28 days of the experiment. During additional investigation of the activity of thiol-disulfide system enzymes it was found that reducing the concentration of GSH in rats with alimentary obesity was due to the lack of thiol-disulfide system enzymes activity: GP and GR, which take part in the regeneration of GSH from GSSG.Conclusion: experimental alimentary obesity is characterized by a reduced redox state in blood, adipose and liver tissues, which is determinative in increasing the free radical reactions and accumulation of highly toxic lipoperoxides in the tissue substrates.Bangladesh Journal of Medical Science Vol.15(3) 2016 p.419-423

scholarly journals Análise comparativa do efeito redutor de colesterol do extrato de semente e casca de uva em ratos Wistar

2016 ◽  
Vol 6 (4) ◽  
pp. 35
Author(s):  
Tharcia Kiara Beserra de Oliveira ◽  
Francisco De Assis Cardoso Almeida ◽  
Flávio Paccelli Silva Sento Sé ◽  
Huggo Luãn Barros Medeiros ◽  
Marina Suênia de Araújo Vilar ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Grape Seed ◽  
Seed Extract ◽  
Skin Extract ◽  
Grape Skin ◽  
Significant Difference ◽  
Calorie Diet ◽  
Experimental Group ◽  
High Calorie Diet ◽  
High Calorie

<p>Os flavonóides são compostos fenólicos presentes na maioria das plantas. Têm um importante papel na prevenção e tratamento da aterosclerose, pois, atuam como agentes antiaterogênicos e hipocolesterolêmicos. Objetivou-se nessa pesquisa estudar o efeito redutor de colesterol do extrato de semente e de casca de uva (<em>Vitis vinifera</em>) em ratos Wistar submetidos a uma dieta hipercalórica (ração acrescida de 10% de óleo vegetal) no período de 20 semanas, a partir da 4ª semana de vida. A amostra, composta por 30 espécimes e distribuída entre o Grupo Controle - A (com apenas dieta hipercalórica, n=10) o Grupo Experimental Casca de Uva - B (dieta hipercalórica + extrato de casca de uva, n=10) e Grupo Experimental Semente de Uva – C (dieta hipercalórica + de extrato de semente de uva, n=10). Os resultados mostraram em média, uma redução dos níveis séricos de colesterol total de 14% e de 17,65% em relação ao grupo controle, respectivamente para os animais com dieta a base de casca e de semente de uva. Não houve diferença estatisticamente significativa na redução dos valores de colesterol entre os animais dos dois grupos, não sendo possível assim determinar qual componente da uva representa a melhor alternativa na prevenção e controle da hipercolesterolemia.</p><p align="center"><strong><em>Comparative analysis of reducing seed extract of cholesterol and effect bark uva in rats Wistar</em></strong><strong><em></em></strong></p><p><strong>Abstract</strong>: Flavonoids are phenolic compounds present in most plants. They play an important role in the prevention and treatment of atherosclerosis, as hypocholesterolemic and antiatherogenic act as agents. This research aimed to study and compare the cholesterol-lowering effect of seed extract and grape skin (Vitis vinifera) in rats subjected to a high calorie diet (increased ration of 10% vegetable oil) in the 20-week period, from the 4th week of life. The sample consisted of 30 specimens and distributed between the Control - A (with only high calorie diet, n = 10) Experimental Group Grape Bark - B (high calorie diet + grape skin extract, n = 10) and experimental group Grape Seed - C (calorie diet + grape seed extract, n = 10). The results showed on average a reduction in serum total cholesterol by 14% and 17.65% in the control group, respectively, for the animals with diet based on bark and grape seed. There was no statistically significant difference in reducing cholesterol levels in the animals of both groups, so it is not possible to determine which grape component represents the best alternative in the prevention and control of hypercholesterolemia.</p>

A Study on the Effect of High Calorie Diet on Stress Alleviation

2012 ◽  
Vol 724 ◽  
pp. 139-142
Author(s):  
Sang Gyun Roh ◽  
Jae Gook Lee
Keyword(s):  
Food Intake ◽  
Adrenal Gland ◽  
Adrenal Glands ◽  
Feedback Inhibition ◽  
Control Group ◽  
Hormonal Change ◽  
Stress Alleviation ◽  
Calorie Diet ◽  
High Calorie Diet ◽  
High Calorie

In this study, we tried to reveal the presence of a cytokine that is regulated by weight, food intake, body fat, the hypothalamus-pituitary-adrenal axis, and corticosterone. The experiment group was given a high calorie diet and the control group was fed a normal calorie diet, and both groups were exposed to various stresses. The behavioral and hormonal change by the kinds and severity of stresses were measured via their immunologic aspect as well as their stress alleviation by voluntary high calorie food intake. After sacrificing the animal, the adrenal gland fat tissues were weighed, and the high calorie diet group showed heavier adrenal glands than those of the control group. In particular, the CVS A group had lighter adrenal gland weight than the Res B and CVS B groups, and the other groups had heavier adrenal glands. The comparison of plasma ACTH concentration to the corticosteroid secretion ratio showed that the CVS A group had decreased ACTH and an increased corticosteroid/ACTH ratio. This fact shows that the adrenal gland was highly active and, in spite of a normal corticosteroid concentration, induced too much negative feedback inhibition.

scholarly journals Research on mechanism of the effect of charred hawthorn on digestive by SCF /c-kit pathway

2021 ◽  
Author(s):  
Qian He ◽  
Cui Liu ◽  
Ting Wang ◽  
Jingying Sun ◽  
Xia Zhou ◽  
...  
Keyword(s):  
Gastric Fluid ◽  
The Body ◽  
Flow Index ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Group Model ◽  
Calorie Diet ◽  
The Brain ◽  
High Calorie Diet ◽  
High Calorie

Abstract Background Hawthorn is the dry ripe fruit of Crataegus pinnatifida Bge in rose family which is a traditional Chinese medicine(TCM) for High-calorie-diet-induced dyspepsia (HC-DID). This study aimed to investigate whether the charred hawthorn coupled with its odor could alleviate HC-DID by brain-gut interaction and stem cell factor(SCF) /c-kit pathway. Methods Rats were randomly divided into 7 groups: control group, model group, cisapride group, hawthorn group (HT), charred hawthorn group (CHT), odor of charred hawthorn (OCHT), CHT + OCHT group. HC-DID rat model was established by high calorie diet for 10 days, hawthorn decoction was administered by gavage, and the self-developed solid drug odor delivery device was used for odor administration. The body weight, food intake, gastrointestinal motility, gastric fluid and gastric acid flow index were determined. HE staining was used to observe the pathological changes of rats. Electrophysiology was used to evaluate the effect of hawthorn combined with its charred odor therapy on electroencephalogram and Electrogastrogram. The SCF and c-kit levels or expressions by immunohistochemistry(IHC), enzyme linked immunosorbent assay (ELISA). Results We found that the odor of charred hawthorn (OCHT) affects the brain (central nervous system) of rats, and hawthorn decoction (raw hawthorn and charred hawthorn) coupled with the odor of charred hawthorn alleviated the symptoms of diet-induced dyspepsia in rats by modulating SCF/c-Kit pathway. Conclusion Thus, we concluded that odor of charred hawthorn has therapeutic effect on dyspepsia and hawthorn may alleviate dyspepsia related symptoms by affecting SCF/c-Kit signal pathway.

scholarly journals Characteristic of Connective Tissue Fibers and Myofibroblasts Interstitium of the Kidneys in Rats Offspring Born to Females with Experimental Metabolic Syndrome

2021 ◽  
Vol 6 (2) ◽  
pp. 37-43
Author(s):  
O. A. Hryhoryeva ◽  
◽  
Ye. V. Korotchuk
Keyword(s):  
Metabolic Syndrome ◽  
Smooth Muscle Actin ◽  
Histochemical Staining ◽  
Collagen Fibers ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Calorie Diet ◽  
Cortical Substance ◽  
High Calorie

According to a number of studies, it is known that a woman's diet during pregnancy may be one of factors in the violation of nephrogenesis. Obesity in pregnancy may increase markers of fibrosis and the accumulation of extracellular matrix in the kidneys. The purpose of the study is to investigate the dynamics of the content of collagen fibers and myofibroblasts in the cortical and medulla of the kidneys of rat offspring. Materials and methods. The research was based according to the international bioethical regulations, 120 albino rats were involved in the study. The first group is experimental (MC-1): rats born to females with an experimental metabolic syndrome and after the start of self-feeding received a high-calorie diet up to 120 days of age. The second group is experimental (MC-2): rats that were obtained from females with an experimental metabolic syndrome, but after switching to self-feeding received a standard diet. The third group is a control group of rats with a standard diet and water regime ad libitum. Mason-tricolor histochemical staining was used to determine collagen fibers, and immunohistochemical reaction using mouse Smooth Muscle Actin monoclonal antibodies (αSMA) was used to detect myofibroblasts. Results and discussion. It was found that the content of collagen fibers in the kidneys of the studied groups gradually increases with increasing of observation period, reaching maximum values on the 120th day of the study, with a statistically significant predominance of values in animals of group MC-1 over control animals in cortical and cerebral matter. Also, control and experimental groups are characterized by a gradual increase in the relative area occupied by αSMA+ cells from the total area of the organ, with maximum values at the end of the study. At the same time, there was a statistically significant predominance of the experimental groups MC-1 and MC-2 in the cortical substance in comparison with control animals. Conclusion. The gradual growth of collagen fibers in the cortical and cerebral substance is probably associated with an increase in the content of αSMA+ myofibroblasts, which according to research are markers of fibrosis

scholarly journals Options of modeling diet-induced and combined metabolic syndrome

2020 ◽  
Vol 19 (6) ◽  
pp. 346-354
Author(s):  
Anna V. Abramtsova ◽  
Natalia V. Efimenko ◽  
Valentina F. Reps ◽  
Agnessa S. Kaisinova
Keyword(s):  
Metabolic Syndrome ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Physical Factors ◽  
Metabolism Regulation ◽  
The Metabolic Syndrome ◽  
Rank Correlation Method ◽  
Experimental Group

Background. Due to the fact that it is not always possible to reproduce all known manifestations of carbohydrate and lipid metabolism disorders, for example, in rodents, it is necessary to verify individual pathogenetic links in animals when modeling the metabolic syndrome in order to select the most optimal natural biotropic factors for studying the effect on them. Aims: To analyze metabolic disorders in the modeling of metabolic syndrome of alimentary genesis in comparison with the combined effect of alimentary factor and chemical agents to select the most appropriate model in the study of natural and weak preformed physical factors. Materials and methods. Metabolic syndrome was modeled in 47 sexually mature outbred white male rats using a hypercaloric diet (HD) of various duration and medications. The first series was the 1st control group (CG1) and the 1st experimental group (EG1). They received HD during 180 days, they were withdrawn from the experiment 30 days after the cancellation of HD. The second series was the 2nd control group (CG2) and three experimental groups. They received HD for 60 days, where the 2nd group (EG2) in association with HD received Mercazolil (10 mg/kg of animal weight) intragastrically for 14 days starting from the 21st day; the 3rd group (EG3) had the same scheme of treatment as in EG2, besides, after taking Mercazolil, they were intramuscularly injected with Nitox 200 at 25 mg/kg once per day during 5 days. Animals of EG2 and EG3 were withdrawn from the experiment 60 days after ending of HD. Animals of the 4th experimental group (EG4) got HD and the same medicine as in EG3. They left the experiment 30 days after ending HD. The content of hormones and biochemical parameters of protein, fat, and carbohydrate in peripheral blood was evaluated using multiple inter-group comparisons and intra-group relationships by Spearmans rank correlation method. Results. There have been detected a large number of biochemical signs of dependence of metabolism regulation on leptin level after long-term HD (180 days), while there were fewer biochemical signs of protein exchange disorder less than with the combined use of HD (60 days) and drugs. Conclusion. It is more appropriate and promising to use a long-term HD as a model of MS close to the natural conditions of its formation, to study the influence of natural and weak preformed physical factors.

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